Re: [Histonet] Tissue Orientation Markers
Hello, Thabk you for the suggestion! Igor On Fri, Mar 26, 2021, 2:29 PM Paula Keene Pierce < pa...@excaliburpathology.com> wrote: > You could use pieces of fixed/processed plant leaf embedded on edge. > > Paula Keene Pierce, BS, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Drive > Norman, OK 73069 > PH 405-759-3953 > http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > > > On Friday, March 26, 2021, 01:23:39 PM CDT, Igor Deyneko via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hello fellow Histonetters, > > Has anyone ever worked with any type of a tissue orientation marker that is > not a dye, but rather some sort of a synthetic product that can be > processed and then sliced in a microtome? > > Previously, we have used plain gut sutures and dyed histogel, which we > processed and made paraffin punch biopsies and then placed it as a tissue > marker along with tissues of interest and processed again. It's quite > cumbersome, so I'm trying to see if there are better options out there? > > Thank you! > > Igor Deyneko > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Orientation Markers
Hello fellow Histonetters, Has anyone ever worked with any type of a tissue orientation marker that is not a dye, but rather some sort of a synthetic product that can be processed and then sliced in a microtome? Previously, we have used plain gut sutures and dyed histogel, which we processed and made paraffin punch biopsies and then placed it as a tissue marker along with tissues of interest and processed again. It's quite cumbersome, so I'm trying to see if there are better options out there? Thank you! Igor Deyneko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Temperature Checks
Hello, We are actually in transition from manual temperature reading to an automated system called CenTrak, managed by Infinite Leap. We are doing a test run with them for our Clinical lab(Hematology, Microbiology, Chemistry and Blood Bank). They installed sensors on freezers, fridges, water baths, incubators and ambient room sensors too. We establish the parameters, go through the validation process. Then we set up alarms/emails when certain equipment is out of range. Everything is centralized in a web page from which we can monitor all sensors. Igor Deyneko Pathology Manager Montefiore Medical Center Bronx, NY On Wed, Oct 7, 2020 at 1:27 PM Simoskevitz, Ricki via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Our facilities department is in charge of this. If there is a problem > they get an alarm from the company. It takes temperature readings every > hour on some equipment. On other equipment it is more often. You can log > in any time and see your temperatures for any day, time etc, > > Ricki Simoskevitz > Scientist Group Leader OI Lab | Eatontown Quality > > Medtronic > Osteoinductivity Lab > 51 James Way| Eatontown, NJ/07724 | USA > Office 732-578-6604|Fax 763-355-1224 > ricki.simoskev...@medtronic.com > Medtronic.com|Facebook|Linkedin|Twitter|YouTube > > > LET'S TAKE HEALTHCARE > FURTHER, TOGETHER > > -Original Message- > From: Amy Self > Sent: Tuesday, October 6, 2020 2:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temperature Checks > > Good Afternoon, > > Do any of you get your temperatures for your equipment and\or > refrigerators monitored by a temperature monitoring company and if so how > do you document this check? > > Thanks in Advance, > Amy Self > Senior Histology Technologist > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > Office: (843) 520-8711 > as...@tidelandshealth.org > Our mission: We help people live better lives through better health. > > > NOTE: > The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to this message and deleting it from your > computer. > Thank you. > [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email > is proprietary to Medtronic and is intended for use only by the individual > or entity to which it is addressed, and may contain information that is > private, privileged, confidential or exempt from disclosure under > applicable law. If you are not the intended recipient or it appears that > this mail has been forwarded to you without proper authority, you are > notified that any use or dissemination of this information in any manner is > strictly prohibited. In such cases, please delete this mail from your > records. To view this notice in other languages you can either select the > following link or manually copy and paste the link into the address bar of > a web browser: http://emaildisclaimer.medtronic.com > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Contamination & Floaters in sections
Dear Histonetters, I wanted to get your input in reconciling and reporting contamination and floaters (C &F) in your slides. Do you even track such instances, of you do, do you differentiate between C&F, what are the metrics that you use and what do you do with that data? I have implemented a very basic tracking system, I get the slides and enter the information on who embedded and sectioned the slide and also the clinician who has performed the procedure per Quality Control department’s orders. Any input regarding the usage of data and what to look for to reduce such instances would be appreciated. Thank you in advance, *Igor Deyneko* Quality Control & Compliance Manager NYU Langone Medical Center Department of Anatomic Pathology 560 First Avenue, New York, NY 10016 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for a Histotech positions in Massachusetts
Dear Histonetters, A professional histotechnologist with nearly 7 years of experience at a small pharmaceutical company is looking for a new position in Mass. I have started a lab from scratch and purchased equipment and trained coworkers on small animal necropsies and organ harvesting. I performed subsequent tissue fixation and tissue processing and paraffin/OCT embedding, I have experience suing a rotary microtome and a cryostat. I have developed biomarker testing panels using IHC, IF and in situ techniques both manually and on DAKO and Ventana autostainers. In addition, I have developed beta gal staining method for frozen tissues and performed specialty staining as well. I have implemented image analysis capabilities in our lab, Aperio and HistoQuest modules and performed image analysis with their respective softwares. If you happen to hire or know someone who is hiring, I would appreciate any leads. Thank you very much. Igor Deyneko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part/Full time positions in Boston
Dear Histonetters, I am looking for either part or full time histologist position in Boston/Cambridge ,MA areas. I have 8 years of lab experience working for 2 mid-size pharma companies. I started off in In-Vivo group and transitioned to strating up a histology core lab from scratch which slowly morphed into a Molecular Pathology dicision. Skills: - Carried out routine staff training on state of the art equipment and innovative staining methods - Performed tissue processing, paraffin and OCT embedding, microtome and cryotome sectioning - Executed standard histochemical, IHC and IF staining - Successfully streamlined procedures for biomarker screens of human and animal tissue samples - Implemented specialty staining procedures as well as a novel Beta-Gal staining method on frozen tissues - Performed qualitative and quantitative image analysis using Aperio ePathology and TissueGnostics modules jn I would appreciate any leads. T Thank you very much, IIgor Deyneko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jobs for MBAs
Dear Histonetters, I am wondering if there are any job opportunities for someone with MBA in Marketing & Operations? I'm a former scientists and was a Histotech, managed a lab and developed from scratch a histology core facility at a medium size pharma company. I went to get an MBA and finishing soon and want to return into science but from Business side. So, wondering if there are any potential opportunities in Histotechnology for MBAs. I would appreciate any leads and advice. Thank you very much. Igor Deyneko D'Amore - McKim School Of Business Northeastern University 360 Huntington Avenue, Boston, 02116 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems with Frozen tissues
I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reticulin Stain(Gomori)
Dear Histonetters! I was wondering if anyone has a good working Gomori stain protocol for Reticulin fibers. i understand that it's a pretty routine procedure; however no one in my lab has ever done it, so I'm wondering if there is a ready-to-use kit already or is it all from scratch. I'm trying to stain liver, spleens and bone marrows.Thank you very much in advance for any advise/help/protocols. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide and Cassette Labeling systems
Hello Everyone! I would like to get your opinion on a good slide and cassette labeling systems. We are trying to incorporate them in our routine histology lab. We are currently using Aperio system as well and maybe wanted to be able to link the slide labeling via bar code to Aperio database as well. Please comment on specifications and also the vendors. Thank you very much. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fibronectin AB for human tissues
Dear Histonetters! I've been looking for a good Fibronecting antibody to stain some human tumors for connective tissues. I've tried 6 from Abcam, all either not working or giving high background. if anyone knows of a good antibody, i would really appreciate it. Thank you. Igor Deyneko Infinity Pharmaceuticals Molecular Pathology Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reducing blood autofluorescence in IF
Hello everyone! I was wondering if anyone knows of an effective method of reducing/ eliminating autofluorescence of blood in IF procedures. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF Doublestaining
Hello Everyone! I'm planning to try some IF co-staining with 2 antibodies, one is a rat-anti-mouse and the other one is rabbit anti-human on a xenograft, each has an appropriate secondary, donkey anti rat and donkey anti-rabbit, conjugated to 488 and 593. Can someone advise the best way to perform such a procedure, I'm afraid the rules of sequential staining might not work due to fluorophore instability with washes. if anyone has performed such type of stain, i would appreciate any tips. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Murine and Human prenatal Fetuses
Dear Histonetters! I am looking for commercially available paraffin sections of the prenatal murine & human fetuses. I would appreciate any information. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decaling mouse joints
Dear Histonetters! I have a project coming up which would require to decalcify mouse hind legs, femur and tibia with joints. Can anyone suggest a good system, either an ion exchange or acid decal(formic acid I've heard works well) and also if anyone has a protocol on fixation and decaling, it would be much appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Labelers
Dear Histonetters! I was wondering if anyone out there is using a slide labeler. I would appreciate any info on models(serial numbers), and vendors. We have high throughput and need a more automated system with minimal time to input data. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HOPE Fixation technique
Dear Histonetters! Recently I cam across this new product offered by Polysciences, called HOPE Fixation( Hepes-glutamic acid buffer mediated Organic solvent Protection Effect). It claims that there is no retreival necessary for IHC and nor formalin either and that it makes even the hardest antibodies to work.So I was wondering if anyone out there has actually tried it out yet. Thank you. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Advice
Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Human VIMENTIN and SMA IHC
Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lymphocytes IHC
Dear Histonetters! I am wondering whether there is a universal IHC marker for different types of lymphocytes, both B and T??? I am trying to start working on inflammation models and have no previous experience with inflammation markers. If anyone knows any, that would be great if they work on paraffin embedded tissues since most CD markers work on frozen only (it's for archival samples). Thank you very much in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alpha Smooth Muscle Actin in Xenografts
Dear Histonetters! I was wondering if anyone out there is working with xenograft tumor models and knows of a good alpha smooth muscle actin antibody that will detect mouse tissue but would give minimal background in tumor cells??? I have tried 6 different ones, 3 from Biocare, 1 from Santa Cruz, and 2 from Abcam, but they all gave me very strong backgrounds. Any information would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Murine Desmoplasia Markres
Dear Histonetters! I was wondering if anyone is working with Desmoplasia markers? I am working with xenograft models (human tumors implanted in mice). So the result is human tumor cells and Murine stroma. I am trying to test various desmoplasia markers found in literature, but almost all of them are for human stroma. So far I got Collagen and Fibronectin working beautifully, but I have problems with the following proteins: alpha Smooth Muscle Actin *Desmin* *GFAP (glial fibrillary actin protein)* *CD 31 (so I'm switching to CD34)* *SPARC* I have a lot of cross-reactivity issues. If anyone knows of antibodies , which are capable of identifying the Murine structures, while keeping the human tissues clean, please let me know. Any information would be highly appreciated! Thank you. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Murine Pathologist Needed ASAP in MA area
Dear Histonetters! I was wondering if there are any or someone knows a veterinary pathologist who has experience working with mouse tissues and possibly Xenograft models? The pathologist we were outsourcing to has moved to a different state. We need one in Boston or nearby Massachusetts area. Any information would be greatly appreciated. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anatomy & Physiology
Hello Everyone! I was wondering if anyone can advise a good book on Mouse and/or Rat anatomy and physiology and also the histology atlas. I looked on-line did not find anything credible. Also, can anyone advise a good HUMAN Anatomy & Physiology Book(s). Tank you in advance. PS. If it's not too hard, pleas include the title, author(s) , and ISBN. Igor Deyneko. Infinity Pharmaceuticals. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Background
Hello everyone! I was wondering if anyone has ever pre-incubated the antibody with normal Mouse IgG to get rid of the unspecific staining. The antibody I use crosreacts with mouse , and both human and mouse tissues stain within a xenograft. I've heard that one can get rid of the mouse staining by incubating with murine IgG. Has anyone ever done that and if so, you mind sharing some info? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology or IHC conferences/workshops
Dear Histonetters! I was wondering where someone knows of any useful conferences or workshops , which deal with histological techniques, or Immunohistochemical techniques or even microscopy techniques( I heard about a place in Cape Cod), coming up in 2009??? Any information would be appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Metanil Yellow and Methyl Green
Dear Histonetters! I have a question regarding a dye called Metanil Yellow, can anyone suggest a good vendor for this dye (I know Sigma and Polyscientific sell it) and what are your impressions? Second question, I have asked long time regarding Methyl Green. I narrowed it down to DAKO ready to use, Polyscientific and also Vector. I did the usual stain for 5 mins and then quick rinse with 95% ethanol, and then 10 dips in series of 2 95%ethanols, 2X 100% and then 2 mins in 2 changes of Xylene and still have inconsistencies. Can someone suggest a good protocol? Thank you very much. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
