Re: [Histonet] Microtome alignment
Amos, indication it is the most practical answer. Histology it is a combination of skills, art, and science. Jaime E Plata MD. MT. HTL (ASCP) --- On Wed, 8/18/10, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Microtome alignment To: sharon.davis-dev...@carle.com, histonet@lists.utsouthwestern.edu Date: Wednesday, August 18, 2010, 6:57 PM Hi, My favorite tool works on all microtomes and is priced perfectly. The knobs on the block holder move the block so when it looks like you are going to cut too much of a block you turn them and, voila, a perfectly aligned microtome. Amos Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine Subject: [Histonet] Microtome alignment To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Licensing?
Contact State of Department of Health Division of Medical Quality Assurance. Be careful in your selection. I did move from Va down here and the housing (realstate) and pay hourly it is no as good we thought. Jaime E Plata BS. MT. HTL (ASCP) Clinical Lab Supervisor Supervisor State of Florida Department of Health --- On Wed, 10/7/09, Feher, Stephen wrote: From: Feher, Stephen Subject: RE: [Histonet] Licensing? To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, "Komal Gada" Date: Wednesday, October 7, 2009, 10:08 AM Here's the link to the Fla DOH Licensure Offices. http://www.doh.state.fl.us/mqa/med-boards.html Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 07, 2009 8:11 AM To: histonet@lists.utsouthwestern.edu; Komal Gada Subject: Re: [Histonet] Licensing? The best thing for you to do is to visit the Florida society for histotechnology website. Google the name. René J. --- On Tue, 10/6/09, Komal Gada wrote: From: Komal Gada Subject: [Histonet] Licensing? To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 6, 2009, 9:17 PM Hello all, I am an ASCP certified Histotechnician (HT), i also have NY state license.Can anyone suggest the best way to obtain a Florida license? Thanks, I appreciate your replies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing techniques
Hello Fellows, Anybody knows or recommend a book or manual for grossing tissue techniques ? please let me know. I am looking for a more practical one on gross description. Thanks you all Jaime E Plata MT.HTL (ASCP) enrri...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Staining vs. paraffin wax problems
Very good, Gudrun I agree with you. I have a seen this crack also on the blocks after embedding them on the cooling stage, and you separate the cassette from the mold. On the tissue and the paraffin, you will see this type of cracking description that you mentioned. In this case I will describe it as Ice (paraffin)-warming crack. Please correct me if I am wrong. This term that I used is some type of global warming reaction phenomenon in paraffin. --- On Sat, 3/28/09, Gudrun Lang wrote: From: Gudrun Lang Subject: AW: [Histonet] Staining vs. paraffin wax problems To: "'Jason Mcgough'" Cc: histonet@lists.utsouthwestern.edu Date: Saturday, March 28, 2009, 4:56 AM Does this look like desert-earth cracks? If yes, this could be a matter of. Water under the edges after sectioning and half-drying. Do you find the? Artefacts in the lower part of your sections related to the drying-direction. (upright)? The section dries and sticks from the outer paraffin-limits to the center. When the rest of the section sits down and shrinks, it could happen that it. tears. (Seen as a water-filled blister.) Be sure, that the water is thrown out before drying, and dry vertically. The other possibility is a too hot waterbath. The sections expand too much, But I think this would be seen all over the section. This can also be seen, if the paraffin infiltration wasn't sufficient. I hope my English is good enough to explain my thoughts. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@bounces@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsbounces@lists.utsboun...@lists.utsouthwestern.edui'mAuftrag von Jason. Mcgough Gesendet: Freitag, 27. März 2009 17:27 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Staining vs. paraffin wax problems We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look. fragmented/chattered. Our pathologists also thought the sections looked like. They might be too thick, but we checked all of our microtomes for thickness. Settings and all are set at 4 micrometers. There seems to be several. Blotches of light staining throughout the tissue. This seems to be only on. Our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is. appreciated. Jason Mcgough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] need controls
GI (small intestine) biopsies are other possibilities associated to skin as control. When you mention Grams, are you referring to Bact's positive and negative.? If this is the case you can make them with blood Agar (microbiology) and contaminate the tissue with them. Or use Large intestine ther will be many you jus need to look for them. --- On Fri, 2/13/09, Steven Coakley wrote: From: Steven Coakley Subject: [Histonet] need controls To: Histonet@lists.utsouthwestern.edu Date: Friday, February 13, 2009, 1:51 PM I work in a DermPath lab. Currently we purchase all our controls. Some are Ok but some, especially the grams are not. We'd like to start sectioning. Our own but all the specimens we get are skins. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology Cell block Preparation
Hello fellows. I am Cyto/ Histology Sup. If there is any one with Non-GN or GN procedure on cell block preparation or methodology please share with me your ideas or procedure. I need also feedback on Thrombin Clot method. Thanks Jaime Plata MT HTL (ASCP) [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
