[Histonet] Influenza A IHC
If anyone is still doing Influenza A IHC testing, could you share your vendor source and catalog number with me? I'm looking to find a better source than what I'm using right now. I don't do it very often (since we have a PCR test) but once in awhile it's requested, and my old antibody just doesn't seem to detect all that it should. Thanks in advance, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline
Hi Sandra, Being a mouse monoclonal, it may not cross-react with canines/felines; the epitope may be specific to human follicular cells only. The polyclonal Thyroglobulin from Dako/Agilent works fine on all the mammals I've tried it on so far (and a frog as well). Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:54 AM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > We had to switch sources (to Novus) for Thyroglobulin > TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet > suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and > pH9 EDTA AR, to no avail. Any suggestions? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Chromogranin A in dogs
Hi Lisa, I just worked up an antibody from abcam; cat. # ab85554 Rabbit polyclonal 1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml) Heat retrieval in pH 6.0 solution 45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen (AEC+) Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:20 AM Lisa Parsons via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Our CgA has stopped working (IHC/P). Does anyone have a source for a CgA > that works in dogs? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Control Slide Longevity
Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Feb 28, 2019 at 12:57 PM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all, > Do any of you have to cut any of you antibody controls > "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We > seldom do a Syanpt., and have found the fresh cut patient tissue has been > positive while the not-so-fresh positive control slide is negative. > Any comments are - as always, are appreciated. > Cheers, > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Not DAB IHC waste
Hello, It depends upon which 'red' chromogen you are using. There are several with different chemical compositions, so you will need to look at the Safety Data Sheets which pertain to the chemical you are using. In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered hazardous. It causes eye and skin irritation, may cause serious eye damage, and may cause cancer. We ship our waste off for proper disposal; it does not go down the water drain. Best regards, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Fri, Feb 22, 2019 at 9:34 AM Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Does anyone use only red or magenta detection for IHCs instead of DAB? We > do not use DAB and are trying to figure out if our waste is hazardous or > not. Any insight would be helpful. > > Thanks, > > Mehndi Helgren > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] guinea pig IHC
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5
Hi Sandy, I get mine from CosmoBio, catalog # KAL-KT022. Needs heat retrieval and I go 1 hr incubation in primary Ab at room temp. Good luck, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Sep 27, 2018 at 1:40 PM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all, > Does anyone know where I can get CD204, Anti-MSR1 > monoclonal, clone SRA-E5? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bordetella IHC
Are there any veterinary labs that do Bordetella IHC and can accept tissue from outside of the US? Asking for a colleague. Thanks in advance. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Adenovirus antibody?
Hello Paula, This isn't an answer to your question, because I do use the Millipore antibody (MAB805) and it works quite well on a Dako platform or done manually. It requires enzyme digestion antigen retrieval. I use it at a 1:400 dilution (45 min) with a polymer detection system (45 min). I'm not sure why it works on one platform but not another. Best of luck, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Aug 3, 2017 at 8:47 PM, Tony Henwood (SCHN) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Paula, > > We use Adenovirus antibody from BioSB (Diagnostic Technology) > Cat Number BSB 5040 > Clone 20/11 and 2/6 > Isotype IgG1/K > Dilution1:200 with citrate retrieval on the Bond 3 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -Original Message- > From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Friday, 4 August 2017 8:50 AM > To: HistoNet > Subject: [Histonet] Adenovirus antibody? > > Hello Listers, > > I am listing to starboard on this one. We are using the Ventana Ultra > staining platform and have had a bit of trouble with our Adenovirus > stains. We have used Abcam and Millipore to no avail. > > Do you have suggestions about another vendor to try? We are a clinical > lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK > too. > > Any suggestions are gratefully accepted. > > Thank you in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EMA/MUC1 IHC on dogs
Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is your vendor? You can reply to me in a private message. Thanks in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
