[Histonet] Cryostats
Hi Everyone, We are looking for a new cryostat and are wondering what everyone has, how they like them, what they recommend, etc! Thank you, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Marking pens coming off cassettes
Hey All! We have been having problems with our marking pens (Secureline/Superfrost). We have two processors, an old VIP and a Thermo Excelsior. The pens seems to smudge on both machines, but especially on the VIP. We tried some sample pens from other companies and they too have smudged. Any ideas whats going on? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histostar Embedding Station
Hi Everyone, Just curious if anyone is using the Histostar Embedding Station? And I am curious to know how you like/dislike it? We have one and have been using it for about 4 weeks and are already needing parts. Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cold plate temperatures on embedding station
Hello All, We just got the Thermo Histostar. Curious to know if there is a CAP requirement for taking the cold plate temperatures on an embedding station? Need to know for quality control purposes. Thank you. Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Napsin A
Hi Loralee, Thanks for your response. What did you use for the multi normal tissue block? Kidney? Also for the multi-tumor block did you just use tumors from organs that you knew were negative for Napsin or did you use both positive and negative tumors? Thanks, Jessica From: "McMahon, Loralee A" To: Jessica Piche ; "histonet@lists.utsouthwestern.edu" Sent: Mon, September 13, 2010 1:29:58 PM Subject: RE: [Histonet] Napsin A We are using a Lung Adenocarcinoma as a daily control. Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained only the expected things and not anything else. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche [jhist...@yahoo.com] Sent: Monday, September 13, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin A
Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Expired Antibodies for Research
Hello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare IntelliPATH Flex Immunostainer
Hi All, We are just wondering if anyone out there is using the Biocare IntelliPATH Flex Immunostainer? If so how do you like it? Any problems? How long have you had it? And any other information you could provide about it would be great. Thanks, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff Quik II
Just wanted to say thanks to everyone who shared their knowledge with me regarding the Diff Quik II. Have a great day! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DFA- Pneumocystis
Good Morning All, Just wondering where people are getting there positive control slides for DFA/Pneumocystis stain? We need to have smeared positive controls. We are doing the stain on BAL slides (cytospins). Does anyone know where we can get them? Thank you, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff Quik II
Good Morning! I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sensitivity and Specificity of Antibodies for IHC
Thanks to everyone who responded to my previous email regarding negative control tissue for antibodies. We are looking for tissue that will not stain with the antibodies I listed. I guess what I should have asked is what kind of procedure are all of you doing to stain for sensitivity and specificity of your antibodies? If anyone would like to share their procedure, that would be great! Hope everyone had a great weekend and thanks again! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Negative Tissue Controls for IHC Validations
Hi Everyone, We are having a hard time figuring out what tissue to use in testing for specificity and sensitivity for the following antibodies: CDX-2 Chromogranin A HCG NSE Synaptophysin H.pylori Factor XIIIa CK 5/6 If anyone wouldn't mind sharing with us what they know we would really appreciate it!! Thanks! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for all the formalin waste responses
Hi All I just wanted to say thanks to everyone who has responded to my question regarding formalin waste going down the drain. I appreciate it! Have a great day! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposal of Formaldehyde
Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure for Immunoperoxidase on a Frozen Section
Hi All, Does anyone have a procedure (using a Dako Autostainer or other Immunostainer) for Immunoperoxidase on Frozen Sections and touch preps, that you wouldn't mind sharing with me? Thanks! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Legal Question
Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Excelsior ES Tissue Processor
Good Morning: Our lab is looking into getting a new processor at some point! We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical? Is it saving you money? and also is anyone using the machine with the xylene free option? Thanks so much! Have a great day! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isopropyl
Good Morning, we are interested in replacing xylene in our lab. Is anyone using graded isopropyl alcohols in there processors instead of xylene and if so how is it working for you? Thanks. Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
