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Joe Nocito, BS, PACM, HTCM (ASCP) QIHC

Dept of Pathology/ 59LSQ/SGVLH

Lackland AFB, TX 78236

joseph.noc...@us.af.mil

 

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[Joe Nocito]

 

 

Joe Nocito, BS, PACM, HTCM (ASCP) QIHC

Dept of Pathology/ 59LSQ/SGVLH

Lackland AFB, TX 78236

joseph.noc...@us.af.mil

 

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RE: [Histonet] Devasting news on 88305TC component

[Joe Nocito]

I was working with a dermpath, who's friend is a dermatologist setting up
their lab. They stopped dead in their tracks. I mean 88305s would be their
bread and butter.

Joe

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, November 02, 2012 12:26 PM
To: Nails, Felton; 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

EXACTLY SO!
POLs started to offer "better prices" to interested colleagues and by doing
so work started to "drain" into POLs with the pointed out result = less work
for Reference labs.
Do you think that large ref. labs like Quest or Lab.Corp were going to "take
that drainage" sitting on their hands?
Sure not! They are business with billions at stake and money to lobby.
This is the result. Blame the power of the lobbyists!
Policies and even sometimes democracy is tainted by big money.
Tell that to the Supreme Court that has ruled that a large company is
a "person". 
René J.



From: "Nails, Felton" 
To: 'Jesus Ellin' ; 'Cristi Rigazio'
; Brendal Finlay
 
Cc: "histonet@lists.utsouthwestern.edu" ;
"Webster, Thomas S."  
Sent: Friday, November 2, 2012 1:01 PM
Subject: RE: [Histonet] Devasting news on 88305TC component

Before you holler political and blame the candidates, ask yourself who was
hurt most by POL's?
Large reference labs.
With this change they will get back the business because it will not be
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist,
most POL's don't.
Just my thought 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, November 02, 2012 11:52 AM
To: 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi
Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in
case anyone wondered both candidates for President are looking for the
middle class!  Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay"
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid
> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl
> t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
> 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> 
> 
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> 
> That is devastating! Do you have a link to this information?
> 
> Sent from my iPhone
> 
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> 
>> Devastating I meant
>> 
>> 
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RE: [Histonet] Background staining on H pylori

[Joe Nocito]

It might be the antibody itself. We are seeing the same problem. My doctor
also thinks that our antibody is picking up normal flora also. Now, when I
QC the slides I have to go by morphology. We have a couple of reps coming by
to boast about their HP. Let you know how that works out.

Joe

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris
Carter
Sent: Monday, November 05, 2012 5:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Background staining on H pylori

Hi,
I'm getting a lot of background staining on my HP's.  We use a Ventana
BenchmarkXT with ultraView DAB.  The problem seems to be escalating of
late, and I'm not sure why.  We use Hollande's on our GI biopsies, and run
them on a shorter run.  Nothing has changed in the processing of the
specimens, or the IHC procedure.  The antibody dispenser is a newer lot,
but not brand new, as we get the larger size due to the high volume of HP's
we run. Any ideas?

Deloris Carter HT(ASCP)
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[Histonet] Calling Lila Adams

[Joe Nocito]

Lila,

Please drop me an email. I have some information for you. Thanks

 

Joe Nocito, BS, PACM, HTCM (ASCP) QIHC

Dept of Pathology/ 59LSQ/SGVLH

Lackland AFB, TX 78236

joseph.noc...@us.af.mil

 

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RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit

[Joe Nocito]

We are having a lively discussion about having 10 known positives and 10
known negatives to validate new antibodies. Many years ago we set up 5 and 5
even before CAP thought of the idea. This year's checklist added the 10 and
10 part, but it is up to the medical director.
What is everyone else doing out there? We are using the Ventana UltraView
detection kits. Everyone who uses these kits know how  expensive they are.
Is 5 and 5 sufficient or should go by CAP recommendations?

Joe Nocito

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
Perez
Sent: Tuesday, September 25, 2012 2:37 PM
To: Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana
Ultraview kit

As far as lot to lot validation that's all we do. Use same control and
compare both.  

Now validating a new detection kit is a whole different story.  Here I just
made a checklist of all the antibodies we do and had the doc sign off on
each stain with the new kit.  
If you want you can do a slide of each with same control one with the iview
and one with the ultraview.
All depends on how your doc wants to validate it.

Vanessa 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, September 25, 2012 1:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana
Ultraview kit

We are trying to decide how to validate our stains when we switch from
Ventana's IView kit to their Ultraview Kit.

I have reviewed the CAP question on this and find the following wording:

The performance of new lots of antibody and detection system reagents are
compared with old lots before or concurrently with being placed into
service.
Note:   Parallel staining is required to control for
variables such as disparity in the lots of detection reagents or instrument
function.  New lots of primary and detection reagents must be
   compared to the previous lot using an
appropriate panel of control tissues.   This comparison must be made on
slides cut from the same control block.

Evidence:   Written procedure and records of verification of new reagent
lots.

For new lots of antibodies we have been running the new lot and comparing
with the previous lot by reviewing the control slide from the old lot to the
new lot.

Is this sufficient?   Wording that bothers me is "appropriate panel of
tissues"

Thanks for your input.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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RE: [Histonet] Picric Acid

[Joe Nocito]

When I was in Texas the second  time ( I was there from 1981-1986 and
returned in 1991. OK so I'm dating myself) , we were preparing a CAP
inspection. I found a brown bottle way in the back of the chemical cabinet.
Lo and behold it was the picric acid I saved from the previous go round. I
said to my supervisor (Hector for those of you who know us) "Hey man, look
what I found? Picric acid." We can't have this around, I'll go put in my
truck". I put more water in the bottle and I carried it around a couple of
days until after the inspection. Yep, miss those days. The things I do for
my buddy.

Joe

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie
O'Connor
Sent: Wednesday, September 19, 2012 3:20 PM
To: tgo...@mt.gov; cing...@uwhealth.org; mcaul...@umdnj.edu;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Picric Acid


There is a lot of published data on the hazards of picric acid - although I
don't think most histo labs have to worry about what they have in house for
trichromes and fixation.  It did cost me more disposal since we used to use
hundreds of gallons a year for fixation of testes.   Finding an alternative
fixative was a good move for us.   
Jackie O'


-Original Message-
From: Goins, Tresa 
To: Ingles Claire ; Geoff ;
histonet 
Sent: Wed, Sep 19, 2012 3:02 pm
Subject: [Histonet] Picric Acid


A WEB site just for historical interest:
http://en.wikipedia.org/wiki/Halifax_Explosion 

e continue to use picric acid in the lab, but only as an aqueous or
saturated 
olution.
The chemical safety guys came out and carefully removed the bottle of 
moistened" picric acid that we had on the shelf for several years - they
were 
ery excited as it was no longer "moist" - Montana is very dry.
Tresa

Original Message-
rom: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] 
n Behalf Of Ingles Claire 
ent: Wednesday, September 19, 2012 1:17 PM
o: Geoff; histonet@lists.utsouthwestern.edu
ubject: RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards
Yes, but why take the chance. There are also other chemicals in the lab the 
icric acid can interact with to make it even more volitile than it was to
begin 
ith. Dynamite other explosives have the same problem. The older it gets the 
ore degraded and unstable it becomes. One never knows if or when. I'd like
to 
void traumatically amputating my arms if possible, thank you.
laire

From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff
ent: Mon 9/17/2012 9:26 AM
o: histonet@lists.utsouthwestern.edu
ubject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards

I am with Wayne on this one. While I have not tried to make it explode it
does 
eem to me that the dangers are hyped beyond reason.
ears ago an old bottle of picric acid would be discovered in a high school 
hemistry lab. Horrors! Call the bomb squad! So it was taken out to a large 
ield, packed with explosives and BOOM! Of course it exploded, it was
surrounded 
ith explosives.
Geoff
On 9/14/2012 8:58 PM, E. Wayne Johnson wrote:
 What danger of Picric Acid are you concerned with?


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RE: [Histonet] ***News Flash***

[Joe Nocito]

My question is "are they being found with empty Lonestar beer cans. Inside
joke. Years ago in Texas, Lonestar Beer was running commercials with
armadillos running across the street carrying Lonestar Beer. See, down here
in Texas, armadillos are usually found as road kill. The commercials had
them belly up holding a Lonestar Beer bottle. I used to have a stuffed one
in my truck. Only in Texas!!!

JTT

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, June 12, 2012 4:24 PM
To: Jay Lundgren; histonet
Subject: Re: [Histonet] ***News Flash***

Epidemic update***
 
Seems the Critters have been tracked back to south florida. Witnesses have
described multiple critters on the sides of highways feet pointing up< small
children assumed the critters had passed from a heart attack>. No injuries
have been reported but one arrest was made. One man was caught surgically
removing the brain from one such critter. Homeland security has been
notified. Stay tuned for further details. 
 


 From: Jay Lundgren 
To: histonet  
Sent: Tuesday, June 12, 2012 2:14 PM
Subject: [Histonet] ***News Flash***
  
BREAKING NEWS
I. C. Critterz
The Associated Press

     Washington, D.C.-  Residents of northern D.C., along with Bethesda,
Silver Springs, and College Park are reporting a wave of armadillo
sightings.  The armadillos are allegedly covered in unusual skin lesions
and missing several toes.  Local zoologists are baffled, as armadillos are
native to the south-central and southeastern United States and are
not normally found in Maryland.  While no attacks have been reported,
authorities are urging residents not to approach any armadillos they might
encounter.
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RE: [Histonet] ***News Flash***

[Joe Nocito]

When I was at the AFIP and I had desk duty, I was afraid to walk through
that 5th floor at night where all the animals were. I always wondered what
they were doing up there. Now I know, adaptable armadillos with leprosy and
ever increasing intelligence scouring D.C. I wonder if they are trained to
attack politicians. Hmm.
I said to my wife with leprosy "hey honey, what's eating you?" 

Joe the toe

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, June 12, 2012 1:15 PM
To: histonet
Subject: [Histonet] ***News Flash***

BREAKING NEWS
I. C. Critterz
The Associated Press

 Washington, D.C.-  Residents of northern D.C., along with Bethesda,
Silver Springs, and College Park are reporting a wave of armadillo
sightings.  The armadillos are allegedly covered in unusual skin lesions
and missing several toes.  Local zoologists are baffled, as armadillos are
native to the south-central and southeastern United States and are
not normally found in Maryland.  While no attacks have been reported,
authorities are urging residents not to approach any armadillos they might
encounter.
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RE: [Histonet] Unregistered techs

[Joe Nocito]

Let me add more fuel to this fire. Will makes strong points. I have been in
this field over 35 years ( has it been that long) Any way. I have worked in
and have managed many labs. I have had registered and unregistered techs.
Some good, some not so good, some I told that their talents would be better
served in another career field. Some people in histology come for the pay,
others for a career. However, I have seen some clinicians, nurses and other
healthcare providers do the same. I went to a neurologist once (emphasis on
once). I was trying to explain to him my lengthy previous medical history,
which has been plagued by  heart problems for years. He was not interested
in that. He wanted to get me in and get me out within the 15 minute time
limit. 
My point is this: I don't care what job you do, there are going to be people
who look at it as a job, others look at as a career. My youngest sister had
some cognitive issues. She worked at minimum wage jobs all her life. One job
was at a laundry mat that had several large accounts. I met her for lunch
one day before I joined the Air Force.  I watched her fold sheets so tight
that they looked like you would cut your fingers on them if you ran them
across the creases. I asked her why she took so much care in folding the
sheets. She looked at me and said "Joey, I do it because anything you do,
you have to do it good. If you ain't gonna do it good, don't do it at all".
I still carry that notion and I hope I have passed that idea onto my
children. When I was in Basic Training, making my bunk, I would always think
of that day with my sister. Consider yourself lucky if you work with more
people who think Histology as a career rather than just a job. I always do. 
I'll get off my soap box now and return you to regular programing.

Joe Nocito

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Chappell
Sent: Thursday, May 24, 2012 6:03 PM
To: Davide Costanzo
Cc: histonet
Subject: Re: [Histonet] Unregistered techs

I have respected Jay's input in the past, but I too must say something.

Without realizing it, and by stating his opinion in a horribly crass way,
Jay has touched upon an important truism.  There are two types of
histologists, those that have a job that pays the bills, and those who have
a career in which they thrive.  Neither are better than the other, both are
needed.  I suspect, however, that the majority of Histonetters -- especially
avid contributors are in the latter group.  I know I am.

Histotechs who approach histology as a job, go into work, embed, cut, stain
and go home.  they are excellent techs, but are just not committed to
expanding the field or doing more than is needed to provide the pathologist
with a perfect slide.  Jay refers to these people as no better than trained
monkeys.  That is a horrible insult with a small (very small) grain of
truth.  One day those histologists will be replaced by a mechanical/robotic
process.  The march of progress is unstoppable.

The career histologist has a much longer life span however.  We analyze and
troubleshoot problems.  We understand or endeavor to learn the organic
chemistry of stains.  We know EXACTLY how a Rabbit Monoclonal antibody is
made.  We know more about the practice of histology than ANY pathologist.
We invent and develop antibodies and special stains.  And we conceptualize
and perfect the instruments that will replace the first group in the future.

Jay, that is why so many are offended.  We don't do this simply because it
is a good paycheck.  We are histologists because we are professionals who
choose this career.  You may be going to a job cutting slides (which is
great and necessary), but we are enjoying our life.

Will Chappell, HTL (ASCP), QIHC, MBA
and histologist by choice, not accident


On May 24, 2012, at 6:48 PM, Davide Costanzo wrote:

> I'm sorry - I cannot let this rest. The comment: "we are just as much
> needed as pathologists, blah, blah,
> blah..." is so upsetting I cannot sit back and listen to that without
> saying something!
> 
> Everyone, regardless of their lot in life, is a very worthwhile part of
the
> whole. Let me ask you a question, since you highly undervalue humans that
> are not MD's - let's say that you are a patient at Hospital X, and you go
> in to have your toenail removed. Who plays a more important role in your
> survival - the Podiatrist or the hospital janitor? I would argue that the
> janitor is more crucial in this instance, for if he/she fails to clean up
> the MRSA from the last patient you could conceivably die. The doctor
solved
> your fungal problem, but the janitor prevented you from getting a
> potentially life-threatening infection. Think before you speak like that -
> everyone involved in your care is critical - and, yes, sometimes th

Re: [Histonet] RE: Interviewing Histotechs...

[Joe Nocito]

yeah, I never did like that the "a monkey can do it" crap. A pathologist 
told me that he could teach a monkey to gross. When the grossing tech messed 
up a case, I was called in and got jumped on. He really didn't appreciate it 
when I told him he better  get that monkey trained quick.


JTT

- Original Message - 
From: "Kim Donadio" 

To: "Jerry Ricks" 
Cc: 
Sent: Tuesday, January 31, 2012 12:46 PM
Subject: Re: [Histonet] RE: Interviewing Histotechs...


Your comment about a monkey hits a nerve. There is a misconception I think 
in our field that yes any monkey off the street can do our job. Well they 
can't. It takes a good amount of knowledge to understand tissues, stains, 
chemical reactions and yes you will need to have some  amount of hand eye 
coordination Skill .


It's the monkey theory that has histotechs jumping up and down into these 
quick almost no hands on programs so they can get a good paying job with not 
much invested I'm afraid. Yes, I'm an expert at sticking my foot in my 
mouth. But if we as a group don't recognize why we are even having this 
debate about testing techs on the most basic of functions.  Then I worry 
about the future of our profession And even healthcare because by god if you 
want monkeys , think monkeys , you will get monkeys!


And darn it. I can't run and hide from this one can I lol

Have a great week!

Kim D
Sent from my iPhone

On Jan 31, 2012, at 1:13 PM, Jerry Ricks  wrote:






Hi Toysha

I think I'm just coming at it from "research mode" not clinical.  Hands on 
Histotechnology is a core part of our work, but just part, and it is 
focused on animal models of cardiovascular disease.  Depending on whether 
the researcher is a postdoc or an undergrad they will have more or fewer 
general lab skills including histo skills.  I haven't met anyone yet who 
did not need some training for embedding of brachiocephalic arteries of 
mice.


I doubt I would do well in a clinical lab.  I've become accustomed to docs 
saying "wow that's beautiful can you teach me how to do that?"  I gather 
in the clinical field it's more like "a monkey can learn how to section." 
Maybe a good monkey could but I doubt it could work up an IHC with a new 
antibody.



Jerry



From: tnma...@mdanderson.org
To: histonet@lists.utsouthwestern.edu
Date: Tue, 31 Jan 2012 10:38:58 -0600
Subject: [Histonet] RE: Interviewing Histotechs...

Jerry,
I agree with you somewhat.  I have met techs that misrepresented 
themselves and said that they could cut or embed, and knew how to operate 
the instruments, but could not produce quality work.  You are right when 
you said that it is different for clinical vs. research. I have almost 
always worked clinical, and noticed that when working with research 
techs, they had a difficult time adjusting to clinical with the time 
frames and quality.
When training new hires, depending on the position I am hiring for, I 
expect to train in the new workflow that they have learned, the new 
instrument they use, not the basic skills.  I only expect to do that with 
a student. Fresh techs are expected to know how to get a section, not cut 
the plastic on the block, embed skin, and set up the h&e stainer.  I 
should only have to go over and orient them on "our procedure" not teach 
the skill.
I have worked various part-time jobs over the years and the first thing I 
ask is 'how many microns do you cut at here'? While 3-4 is the standard, 
some labs want everything at 3, or some at 4.  I know how to cut, but 
like you it takes about 2 weeks to get used to the new instrument. That's 
fine, but I don't expect to have to teach the tech how to embed a skin or 
cut a kidney biopsy. Not for an experienced tech, unless they have never 
encountered it.  That has to be made known during the interview.
Yes, a cutting test is good, I have seen registered techs not make it 
past probation (90 days) because they could not cut. It would have saved 
the company time and MONEY if a test could have been given. Asking if 
they can cut a kidney biopsy, or embed a skin would be good as well. I 
can't go back and get more epithelia or ask for another pass through the 
kidney.




Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
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Message: 5
Date: Mon, 30 Jan 2012 11:13:11 -0800
From: Jerry Ricks 
Subject: [Histonet] Interviewing Histotechs...
To: 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


I gather it is different in clinical labs than in research labs.  In 
clinical labs there is an emphasis on quantity and speed.  In research 
the emphasis is on doing good experiments.  Our "patients" are almost 
always deceased or shortly about to be so there is no urgency of 
diagnosis factor.  For us, "diagnosis" means making precise measurements 
else some scientists looking at an image and asking each other "what 
the?"


Anyway I always

Re: [Histonet] Interview Questions

[Joe Nocito]

I would appreciate that Tony
- Original Message - 
From: "Tony Henwood (SCHN)" 
To: "'Joe Nocito'" ; "joelle weaver" 
; ; 
; ; "Histonet" 


Sent: Thursday, January 26, 2012 3:39 PM
Subject: RE: [Histonet] Interview Questions


Joe,

I would never wear a denim miniskirt!

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito

Sent: Thursday, 26 January 2012 11:14 AM
To: joelle weaver; trathbo...@somerset-healthcare.com; 
billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; Histonet

Subject: Re: [Histonet] Interview Questions

I used to give a 10 question test on general histology. I also had the 
expected answers written down and on my copy. Was accused once of being a 
racist. What saved me was having the answers in front of me. The person 
didn't get one answer correct. I had a couple of embedding questions, some 
cutting, special stains, immunos and some QC questions. I gave the 
interviewee the test while I was reviewing their resume. I would also see 
what their facial expressions were too. I had one person tell me they didn't 
do specials or immunos and didn't like embedding either. When I asked if 
they liked filing blocks and slides, they really would rather have a lab 
aide do it. This person didn't have to finish the test. Too make matters 
worse, she wore a denim miniskirt to boot. Just my three cents


Joe
- Original Message -
From: "joelle weaver" 
To: ; ;
; "Histonet" 
Sent: Wednesday, January 25, 2012 12:02 PM
Subject: RE: [Histonet] Interview Questions



Love this! I always want to do demonstration during technical interviews, 
but usually get "shot down" from managers and argued with in general,  as in 
people don't feel that they should have to "prove" they can do histology.
This perception,  I never got, because I always saw it as in a job 
interview-in what other situation are you more trying to "prove" or impress 
with your knowledge, attitude, skills and experience?  If you do bench work, 
you can tell in just a few minutes of observation much more information than 
you could get with quite a few questions. To be fair, I take into account 
nervousness, being closely observed, and lack of familiarity with equipment 
etc. I don't know, I think its fair if those are important skills to the 
position/role. Was not sure if Sara's job was mostly technical though, so 
thought I might keep it general.


Joelle Weaver MAOM, (HTL) ASCP

http://www.linkedin.com/in/joelleweaver

> From: trathbo...@somerset-healthcare.com

To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu;
histonet@lists.utsouthwestern.edu
Date: Wed, 25 Jan 2012 17:47:01 +
Subject: RE: [Histonet] Interview Questions
CC:

If your replacement will be doing actual histology, will your
institution permit the applicant to embed and cut? Can you sit down at
a multi-head scope and review slides with them?
What will the person be responsible for? Do they have experience with
all of these tasks? What would they do in a crisis situation (you can
make up one yourself that would be plausible).
People who volunteer in their personal lives, may do the same at work.
Ask how they juggle their schedule though, if there is a lot going on
in their personal lives. Be careful with how you ask these questions
though. Your HR department should be able to give you guidance in how to 
phrase things.

Good luck.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Wednesday, January 25, 2012 12:19 PM
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Interview Questions

It would seem that questions like "How do you feel about cannibalism?"
might also be out but might be far more helpful; than "phone" questions.


On the serious side, when I was much younger I hired a person who was
able to answer all the right "histo" questions and so I hired him. He
turned out to be a poser, who, shortly after I fired him showed up at
a local university with a lab coat that listed him as "Dr." He had
indeed worked in a histo lab, but as a lab assistant, and so the the
understanding of what a histologist does was well rehearsed. (BTW, it
topok me about two weeks to catch on, though the more experienced
techs in the department figured it out almost right away)

To be fair, it was during a time in hiring history when HR departments
we

Re: [Histonet] Interview Questions

[Joe Nocito]

I used to give a 10 question test on general histology. I also had the 
expected answers written down and on my copy. Was accused once of being a 
racist. What saved me was having the answers in front of me. The person 
didn't get one answer correct. I had a couple of embedding questions, some 
cutting, special stains, immunos and some QC questions. I gave the 
interviewee the test while I was reviewing their resume. I would also see 
what their facial expressions were too. I had one person tell me they didn't 
do specials or immunos and didn't like embedding either. When I asked if 
they liked filing blocks and slides, they really would rather have a lab 
aide do it. This person didn't have to finish the test. Too make matters 
worse, she wore a denim miniskirt to boot. Just my three cents


Joe
- Original Message - 
From: "joelle weaver" 
To: ; ; 
; "Histonet" 

Sent: Wednesday, January 25, 2012 12:02 PM
Subject: RE: [Histonet] Interview Questions



Love this! I always want to do demonstration during technical interviews, 
but usually get "shot down" from managers and argued with in general,  as in 
people don't feel that they should have to "prove" they can do histology. 
This perception,  I never got, because I always saw it as in a job 
interview-in what other situation are you more trying to "prove" or impress 
with your knowledge, attitude, skills and experience?  If you do bench work, 
you can tell in just a few minutes of observation much more information than 
you could get with quite a few questions. To be fair, I take into account 
nervousness, being closely observed, and lack of familiarity with equipment 
etc. I don't know, I think its fair if those are important skills to the 
position/role. Was not sure if Sara's job was mostly technical though, so 
thought I might keep it general.


Joelle Weaver MAOM, (HTL) ASCP

http://www.linkedin.com/in/joelleweaver

> From: trathbo...@somerset-healthcare.com
To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; 
histonet@lists.utsouthwestern.edu

Date: Wed, 25 Jan 2012 17:47:01 +
Subject: RE: [Histonet] Interview Questions
CC:

If your replacement will be doing actual histology, will your institution 
permit the applicant to embed and cut? Can you sit down at a multi-head 
scope and review slides with them?
What will the person be responsible for? Do they have experience with all 
of these tasks? What would they do in a crisis situation (you can make up 
one yourself that would be plausible).
People who volunteer in their personal lives, may do the same at work. Ask 
how they juggle their schedule though, if there is a lot going on in their 
personal lives. Be careful with how you ask these questions though. Your 
HR department should be able to give you guidance in how to phrase things.

Good luck.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
Bill

Sent: Wednesday, January 25, 2012 12:19 PM
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Interview Questions

It would seem that questions like "How do you feel about cannibalism?"
might also be out but might be far more helpful; than "phone" questions.


On the serious side, when I was much younger I hired a person who was able 
to answer all the right "histo" questions and so I hired him. He turned 
out to be a poser, who, shortly after I fired him showed up at a local 
university with a lab coat that listed him as "Dr." He had indeed worked 
in a histo lab, but as a lab assistant, and so the the understanding of 
what a histologist does was well rehearsed. (BTW, it topok me about two 
weeks to catch on, though the more experienced techs in the department 
figured it out almost right away)


To be fair, it was during a time in hiring history when HR departments 
were not willing to give useful reference data and there were only a 
handful of questions they would even ask when checking. None of them were 
particularly useful or telling. For inistance, they would not ask if the 
person was an histo tech, but would simply ask, did he indeed work at your 
institution?


The place where I worked required little or nothing for proof of 
experience. There was no background check either.


Today, however, reference checking is a lot easier and more reliable.

I guess my point here is that a good reference check needs to be done as 
well weeding them out by histo questions.  I'm sure your HR folks will do 
a fine job of this.


Also, once you have determined that they actually have the skills, or a 
realistic potential of gaining them, questions concerning dynamics of 
interaction are appropriate, though may lead to wrong impressions in the 
mind of the applicant.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, 
Sara

Sent: Wednesday, January 25, 2012 10:52

Re: [Histonet] time off

[Joe Nocito]

I start with the holidays. We rotate the major ones every year. When I set 
up the private lab I used to work at, I had it put in the H.R. policy that 
an employee could take 2 of 3 end of year holidays (Thanksgiving, Christmas, 
New Year's) on a rotating basis. If no one else put in for those times, 
then I would grant that request. I went out only 6 months at a time. We were 
expanding exponentially and I couldn't project what the work load would be 
like next week, never mind 6 months from now. All the other holidays were 
not a problem.


JTT
- Original Message - 
From: "Amber McKenzie" 

To: 
Sent: Tuesday, January 03, 2012 3:09 PM
Subject: [Histonet] time off



Those of you who are supervisors, how do you handle your co-workers asking 
for time off?  I have 2 employees that have asked off already (jan 3rd) for 
every day they want off for the entire year!  Do you grant them the days off 
since no one else has asked off yet, or tell them it's not fair to 
continuously get off around every holiday by asking off  5 - 12 months in 
advance?


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Re: [Histonet] Has anyone else noticed

[Joe Nocito]

I wonder if it works with Scotch. Hmmm, seems like an experiment brewing.
JTT

- Original Message - 
From: "Tony Henwood (SCHN)" 
To: "'Della Speranza, Vinnie'" ; 


Sent: Monday, December 19, 2011 4:04 PM
Subject: RE: [Histonet] Has anyone else noticed


I keep trying to verify this scientific discovery but everything is blurry 
by the time I get to the bottom of the bottle, Oh sorry I need to look in 
the glass?


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della 
Speranza, Vinnie

Sent: Tuesday, 20 December 2011 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Has anyone else noticed




From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!

-Original Message-
From: "histonet-boun...@lists.utsouthwestern.edu" 


Sent: 12/19/2011 10:31 AM
To: "histonet@lists.utsouthwestern.edu"
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water 
will turn a nice cabernet sauvignon residue blue (reminiscient of 
hematoxylin staining solution) ??



Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





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Re: [Histonet] pathology grossing stations

[Joe Nocito]

Bob,
try Mopec. When I was at this year's NSH in Cinci, they had a few different 
sizes and configurations.


Joe the Toe
- Original Message - 
From: "Bob Richmond" 

To: "Histonet@lists.utsouthwestern.edu" 
Sent: Thursday, December 08, 2011 2:19 PM
Subject: [Histonet] pathology grossing stations



A laboratory I'm doing some work for is planning to remodel within two
months. We see about 5,000 specimens a year, with a moderate number of
hospital major specimens including placentas. Currently we have no
grossing station, and this may be an opportunity to get one. It will
be installed in a windowless unventilated room, like our present sink
setup. I don't know the dimensions of the space available. I suspect
the decision will be made on the basis of price, with us having very
little input.

What currently available grossing station do you have? Do you like it?

Bob Richmond
Samurai Pathologist
Knoxville TN

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Re: [Histonet] RE: 15 years of Histonet

[Joe Nocito]

Can't agree more. I mean, where would Joe the Toe be without the Histonet?
By the way, just read the reviews from the lecture my buddy Hector and I 
gave in Cinci this year. One of the critiques stated that Hector and I are 
the biggest egomaniacs this side of space. Wow, I've been called a lot of 
things in my life, but an egomaniac? Really? That hurt, right to the core.


JTT
- Original Message - 
From: "Morken, Timothy" 

To: 
Sent: Monday, November 14, 2011 10:32 AM
Subject: [Histonet] RE: 15 years of Histonet


Linda Wrote "By the way,  Histonet is coming up on its 16th year anniversary 
(wow!) and  there are currently 3543 members on the list (from >30 countries 
as of my last tally)."


That is really great. I agree with John Kiernan that Histonet really opened 
up the histology community. Back in the "old" days you were lucky if you 
knew a few techs outside your own lab, and met a few at the occasional state 
or national meeting. Most histotechs were very isolated. Since Histonet that 
has all changed. Now we are all just an email away from getting just about 
any question answered. I am continually astonished at the breadth of 
knowledge out there AND the breadth of the field.


I think I heard about Histonet from NSH. In any case it was around 1997 and 
I was working in Saudi Arabia. Histonet was a fantastic resource and I even 
ended up getting a job back in the US through contacts I made on Histonet 
(remember that Jeannine?).


Since then I peruse it almost daily and occasionally post. In any case it is 
always interesting to see what the unofficial "topic of the day" is!


Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda 
Margraf

Sent: Friday, November 11, 2011 12:01 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Unsubscribing from Histonet

Dear Histonetters:

Hi, I haven't put out many messages on the list recently.  I have just been 
quietly administering the list (and occasionally removing people who ask to 
be unsubscribed as my time allows).   By the way,  Histonet is coming up on 
its 16th year anniversary (wow!) and  there are currently 3543 members on 
the list (from >30 countries as of my last tally).


As it keeps coming up,  if you wish to unsubscribe or modify your account 
options,  go to this link:



http://lists.utsouthwestern.edu/mailman/options/histonet

You will need to request your password if you don't know or remember it.  I 
tried to get this link added to the bottom of all the messages but 
apparently that is not possible with the current software.  If you have 
trouble getting off the list, let me know.If your email address has been 
changed at your organization, you may have trouble posting messages or 
unsubscribing. If so, let me know (but provide me with the old address that 
is on the membership list) and I'll try to help.

Thanks,
Linda M
Histonet administrator

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files or attachments transmitted with it contains
information that is confidential and privileged. This information is 
intended only for the use of the 
individual(s) and entity(ies) to whom it is addressed. If you are the 
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disclosures are prohibited without proper authorization. If you are not the 
intended recipient, any 
disclosure, copying, printing, or use of this information is strictly 
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violation of federal or state law and regulations. If you have received this 
information in error, 
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hereby claim all 

applicable privileges related to this information.



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Re: [Histonet] Where are you, JTT?

[Joe Nocito]

My son is heavily in high school activities now. I have to drive him to all 
these school events. I know I'll probably pay for this later down the road, 
but I'm actually pushing him to get his driver's permit so he can drive. by 
time we get home, it's time for my beauty sleep. If you saw me in Cinci, 
you'd understand that I need all the help I can get.


JTT
- Original Message - 
From: "Breeden, Sara" 

To: 
Sent: Wednesday, November 02, 2011 9:08 AM
Subject: [Histonet] Where are you, JTT?


This being Halloween Week, you must be lurking out there somewhere...



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



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Re: [Histonet] RE: Luciferase IHC!

[Joe Nocito]

I'm here.  I was asking my Priest the steps to perform an exorcism, although 
the Catholic Church doesn't perform these rituals any longer. I have all the 
materials, readings and "exorcism paraphernalia" Just let me know where and 
when. Just make sure there is a good bottle of Scotch available, just in 
case all else fails.


JTT
- Original Message - 
From: "Marsh, Nannette" 
To: "'Randolph-Habecker, Julie'" ; 


Sent: Wednesday, November 02, 2011 11:31 AM
Subject: [Histonet] RE: Luciferase IHC!


This is great.  I've enjoyed the story line and witty comments and remarks. 
Thanks,Nanne


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
Randolph-Habecker, Julie

Sent: Wednesday, November 02, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luciferase IHC!

Thanks for the help!
We actually tried antigen retrieval in Holy water in a pressure cooker for 
10 minutes. I even brought in an old priest and a young priest to perform 
the staining but no luck! Sorry, no vendor for the holy water - the old 
priest makes his own.


I enjoyed the laugh! This project has been hell!

Thanks,

Julie

Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: "Beckham, Sharon" 
Subject: RE: [Histonet] Luciferase IHC
To: "'O'Donnell, Bill'" , Kim Donadio
, "Randolph-Habecker, Julie"
, "histonet@lists.utsouthwestern.edu"

Message-ID:
<2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org>

Content-Type: text/plain; charset="iso-8859-1"

You know sometimes I wish these emails had a "LIKE" button on them.   I 
found this quite funny and wanted to "LIKE" it.  Too much facebook for me!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
Bill

Sent: Wednesday, November 02, 2011 8:19 AM
To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC

Actually, I have found that holy water reduces its sensitivity, greatly 
diminishes its signal and will destroy its reactivity. (Listen closely and 
you will hear the slide scream and growl and tell you no one likes you) 
(It's only Wednesday, what'll Friday look like?)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio

Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC

You have to soak it in holy water, then all H311 will break out

Sorry, I couldnt resist.





From: "Randolph-Habecker, Julie" 
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If so, 
what antibody did you use.




Thanks!!



Julie



Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabe...@fhcrc.org



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End of Histonet Digest, Vol 96, Issue 3
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Re: [Histonet] AFIP closing

[Joe Nocito]

I was stationed at AFIP from 1986-91.  I was in the Immuno lab. I thought I 
was doing "imunos" when I was stationed in San Antonio, TX (remember the 
Dako kits?) I wasn't even close to the things that AFIP was doing. We were 
making our own rabbit antibodies. Made up our own link and label from 
scratch. Our DAB was order in 5gm powder bottles and we had to prep it under 
the fume hood in full PPE. I really learned about immunos there. Even tried 
a new method using a lead-based solution in a microwave to open up binding 
sites in FFPE tissue. Worked with a new automated immuno stainer from 
Fisher. Think it was called the "Brigoti" machine.

I learned  a lot there, but was ecstatic to return to San Antonio.

Joe
- Original Message - 
From: 

To: 
Sent: Friday, September 09, 2011 10:04 PM
Subject: [Histonet] AFIP closing





Hi Histonetters, especially Joyce as it seems we may be the 'same vintage' - 
but I have to chime in as an old-timer and say how sad I am too about the 
AFIP. My histology career essentially started there. I was fortunate enough 
to have been trained there in Lee Luna's time, and knew the folks who 
literally "wrote the book" (you all know which one I mean, I'm sure, the 
Histo Bible). I was also at Walter Reed hospital - which also closed just in 
the past few days - which was then housed within the AFIP building, on the 
same floor actually. It sure makes me feel both blessed for such excellent 
training and at the same time "outmoded" for these institutions to be gone. 
There is little to add after that, but I enjoy reading the posts on Histonet 
just the same.


Brigit in CA
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Re: [Histonet] Congo Red control slides

[Joe Nocito]

Greetings Histoland,
in a pinch, we've also used mayonnaise. We opened one of the small packets 
you get with sandwiches and smeared a little of it onto a slide and there 
you go. Also, if you can obtain a fresh pierce of kidney with Renal Cell 
carcinoma, that will work also.


Joe
- Original Message - 
From: "mesruh turkekul" 

To: 
Sent: Tuesday, August 30, 2011 2:45 PM
Subject: [Histonet] Congo Red control slides



Hi,

I would like to know if anybody is purchasing positive control tissue 
slides

for congo red staining.
Can you suggest any vendor and catalog number?

Thanks,
Mesru Turkekul
mskcc.org
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Re: [Histonet] RE: New CAP question

[Joe Nocito]

Greetings all,
I contacted CAP just before my on-site inspection last year. I was told that 
all validation studies had to be on file until the antibody/ special stain/ 
or equipment was no longer used. Sad to say. Now, I understand for 2012, CAP 
has added another "lab specific" checklist. My understanding is that some of 
the General Checklist questions will now be department specific. It's like a 
subset of the General Checklist. I was told by a co-worker, I haven't seen 
anything in print yet, but come on people. A General  department checklist 
from the General checklist. Then why have a General Checklist? Let's keep 
adding checklists because we can? This is what happens when a monopoly is 
formed. I'm going to start my own inspection company. I'm going to start 
from the toes and work my way up.


JTT
- Original Message - 
From: "Carol Bryant" 
To: "'Vickroy, Jim'" ; 


Sent: Thursday, August 25, 2011 11:09 AM
Subject: [Histonet] RE: New CAP question


Please respond to all.  I would like the information also.
Thank you,
Carol

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim

Sent: Thursday, August 25, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New CAP question

One of the new CAP questions is ANP.22976 ER/PgR validation.

If the laboratory performs immunohistochemistry for estrogen receptor and/or 
progesterone receptor as a prognostic/predictive marker on breast carcinoma, 
the laboratory has documented appropriate validation for the assays.  In the 
note it says should include a minimum of 40 cases and validation should be 
performed by comparing the laboratory's results with another assay that has 
been appropriately validated.


We have been doing ER/PR's for over ten years.  Originally we compared our 
ER/PR testing with the old immunology method that used frozen breast tissue. 
We also compared our ER/PR results with another hospital.  Problem is that 
this has been over ten years and we do not keep quality control records that 
long.   Am I missing something?
I know we use the FDA approved protocol from Ventana on our Ventana 
Benchmark XT.
Should we do another validation study using Ventana or another hospital that 
is using the FDA approved method?   Anybody understand what CAP is wanting 
and how to accomplish this?


James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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Re: [Histonet] Sox 10

[Joe Nocito]

would that be the Red Sox or the White Sox. Sorry, couldn't resist. Had a 
very bad day


Joe
- Original Message - 
From: "Thomas Jasper" 

To: 
Sent: Wednesday, July 27, 2011 11:01 AM
Subject: [Histonet] Sox 10


General question -



Is anyone out there running Sox 10 on the Ventana Benchmark XT?  Any
dilutions or protocols would be appreciated.

Thanks,

Tom Jasper

Central Oregon Regional Path

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[Histonet] Denatured Alcohol

[Joe Nocito]

Greetings and Salutations Histoland,
I have a question about denatured alcohol. I work in a government facility 
and absolute alcohol (200 proof) is still considered a controlled substance. 
This requires a monthly inventory by someone from another department.  Years 
ago (ok many, many years ago) I remember that 200 proof had an IRS sticker 
covering the cap.
The alcohol we have is "denatured alcohol, 200 proof",  which according to 
the MSDS is cut with kerosene. There is no IRS sticker on the bottles.
Question #1- If the alcohol is cut with something other than ethanol, ( which 
usually it's cut with methanol) is it still 200 proof?
Question #2-  If it is "denatured", it is considered not suitable for drinking. 
If the substance is not suitable for drinking, then why would it be considered 
a controlled substance?
See my dilemma? We would like to get it removed from our "controlled" substance 
list, but we need a reliable source. The company (whom shall remain nameless 
because of my past history of inflaming vendors) was useless. I don't think the 
government accepts Wikipedia as an authoritative source. Thanks 

JTT
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Re: [Histonet] negative controls on immunos

[Joe Nocito]

yes. I'm in the beginning stages of validating a new immuno machine. Because 
we have over 120 antibodies, I'm guessing about 2500 slides. Guess who pays 
for that testing? Certainly not CAP. I hate monopolies. Even though I know 
labs who dropped CAP and went with JCAHO, CAP is still a monopoly. Let's 
revolt. Seems to be catching on in the Middle East.


Joe
- Original Message - 
From: "Angela Bitting" 
To: "Diana McCaig" ; "Greg Dobbin" ; 
; "Joyce Weems" 

Sent: Wednesday, March 02, 2011 10:42 AM
Subject: RE: [Histonet] negative controls on immunos


Fun no, impractical yes.


"Weems, Joyce"  3/2/2011 10:30 AM >>>

The CAP guidelines are pretty clear.  Copied from latest checklist..
Isn't this fun???! j:>)



ANP.22570   Phase IIN/A  YES  NO

Are appropriate negative controls used?

NOTE:  Negative controls must assess the presence of nonspecific
staining in patient tissue as well as the specificity of each antibody.
Results of controls must be documented, either in internal laboratory
records, or in the patient report. A statement in the report such as,
"All controls show appropriate reactivity" is sufficient.

A negative reagent control is used to assess nonspecific or aberrant
staining in patient tissue related to the antigen retrieval conditions
and/or detection system used.  A separate section of patient tissue is
processed using the same reagent and epitope retrieval protocol as the
patient test slide, except that the primary antibody is omitted, and
replaced by any one of the following:

■   An unrelated antibody of the same isotype as the primary
antibody (for monoclonal primary antibodies)
■   An unrelated antibody from the same animal species as the
primary antibody (for polyclonal primary antibodies)
■   The negative control reagent included in the staining kit
■   The diluent/buffer solution in which the primary antibody is
diluted

In general, a separate negative reagent control should be run for each
block of patient tissue being immunostained; however, for cases in which
there is simultaneous staining of multiple blocks from the same specimen
with the same antibody (e.g., cytokeratin staining of multiple axillary
sentinel lymph nodes), performing a single negative control on one of
the blocks may be sufficient provided that all such blocks are fixed and
processed identically.  This exception does not apply to stains on
different types of tissues or those using different antigen retrieval
protocols or antibody detection systems.  The laboratory director must
determine which cases will have only one negative reagent control, and
this must be specified in the department's procedure manual.

The negative reagent control would ideally control for each reagent
protocol and antibody retrieval condition; however, large antibody
panels often employ multiple antigen retrieval procedures. In such
cases, a reasonable minimum control would be to perform the negative
reagent control using the most aggressive retrieval procedure in the
particular antibody panel.  Aggressiveness of antigen retrieval (in
decreasing order) is as follows:  pressure cooker; enzyme digestion,
boiling; microwave; steamer; water bath.  High pH retrieval should be
considered more aggressive than comparable retrieval in citrate buffer
at pH 6.0.

It is also important to assess the specificity of each antibody by a
negative tissue control, which must show no staining of tissues known to
lack the antigen.  The negative tissue control is processed using the
same fixation, epitope retrieval and immunostaining protocols as the
patient tissue. Unexpected positive staining of such tissues indicates
that the test has lost specificity, perhaps because of improper antibody
concentration or excessive antigen retrieval. Intrinsic properties of
the test tissue may also be the cause of "non-specific" staining.  For
example, tissues with high endogenous biotin activity such as liver or
renal tubules may simulate positive staining when using a detection
method based on biotin labeling.

A negative tissue control must be processed for each antibody in a
given run.  Any of the following can serve as a negative tissue
control:

1.  Multitissue blocks.  These can provide simultaneous positive
and negative tissue controls, and are considered "best practice" (see
below).
2.  The positive control slide or patient test slides, if these
slides contain tissue elements that should not react with the antibody.
3.  A separate negative tissue control slide.

The type of negative tissue control used (i.e., separate sections,
internal controls or multitissue blocks) should be specified in the
laboratory manual (refer to ANP.22250).

Multitissue blocks may be considered best practice and can have a major
role in maintaining quality.  When used as a combined positive and
negative tissue control as mentioned above, they can serve as a
permanent record documenting the sensitivity and specificity of ev

Re: [Histonet] use of destained sections in IHC?

[Joe Nocito]

I wouldn't decolorize the slides. Just remove the coverslip and hydrate to 
water. Any antigen retrieval should take out the Eosin. You're going to 
counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an 
informal study. I took 10 antibodies, stained two sets of controls H & E. 
One set, I used 1% acid-alcohol to decolorize the slides, the other set I 
just rehydrated to water. The slides that were placed in acid-alcohol either 
had no staining or a marked decrease in intensity.


Joe
- Original Message - 
From: "Walter Benton" 
To: "Robin Dean" ; 


Sent: Wednesday, February 23, 2011 2:00 PM
Subject: RE: [Histonet] use of destained sections in IHC?


It will work and if you are performing antigen retrieval then you won't need 
to decolorize the slide, just rehydrate and perform per you usual protocol.


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean 
[robin_d...@compbio.com]

Sent: Wednesday, February 23, 2011 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?

Hi all,



Is it possible to de-stain H&E stained  paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if it won't work.
I would appreciate any suggestions anyone might have



Thank you,



Robin



Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com



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[Histonet] Thank you

[Joe Nocito]

Just wanted to thank you all for your advice, likes, dislikes and experiences. 
I am really honored to be part of this group. Seriously. It's refreshing.

Joe
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[Histonet] Tissue Processors

[Joe Nocito]

Greetings all,
if you had to purchase new tissue processors, which one would you choose? 
Microwave technology is out of the question. Are Sakura's still a good buy? 
We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help

Joe
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Re: [Histonet] Benchmark Problems

[Joe Nocito]

I would also see if Ventana can send you some test slides to test the 
heating. At my old lab, we had a problem with the heating plates. They 
repaired the entire platform. Just a thought.


Joe
- Original Message - 
From: "Mark Tarango" 

To: "Dessoye, Michael J" 
Cc: 
Sent: Wednesday, February 09, 2011 10:58 AM
Subject: Re: [Histonet] Benchmark Problems



Hi Mike,

Did you wash the bottles and rinse them and then pump the water through 
the

instrument before adding reagents and purging all again?  We had a problem
like this about 2 weeks ago.  I think someone loaded the wrong bulk 
reagent
onto the instrument.  Cleaning it, purging with water and adding newly 
made

bulk reagents fixed the problem for us.

Mark

On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J 
wrote:



Hello,

We started having a strange problem with our Ventana Benchmark XT.  Out 
of

the blue, our slides stopped staining.  With most antibodies we get no
reaction, some that were very strongly positive are now only very lightly
staining.  They do appear to counterstain OK.  Following Ventana's
recommendations we completely changed out all of our bulk fluids, purged 
the

lines, changed antibodies and DAB kits.  The last shot is some kind of
instrument issue that's not triggering an error.  Anyone ever see 
anything

like this?

Mike Dessoye
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 
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Re: [Histonet] IHC validation

[Joe Nocito]

fortunately, we don't perform prognostic markers. The doctors don't even 
want to venture down that road. When I started working here, that's the 
first ting I mentioned. We can save some much money by doing them in-house. 
That went over like a lead balloon. On a side note, the staff now were 
residents when I retired from active duty. Talk about feeling 
ldd.


Thanks guys for the tips. I will pass them on to the powers at be and let 
them decide.


JTT
- Original Message - 
From: "Morken, Tim" 
To: "'Liz Chlipala'" ; "Weems, Joyce" ; 
"Joe Nocito" ; "Histonet" 


Sent: Tuesday, February 08, 2011 5:37 PM
Subject: RE: [Histonet] IHC validation


When changing instruments you are validating the instrument, not the test. 
For each antibody you just need to run parallel tests in each instrument 
showing equivalence.


However, if you change the instrument and ALSO change the antibody and/or 
detection system, Antigen retrieval, etc, then you need to revalidate the 
test.


For most antibodies (Class I FDA exempt, ancillary tests) that involves 5-10 
positive and negative cases. A small tissue array can suffice. You should do 
reproducibility tests for a few antibodies - 5-10 identical slides on one 
run, 5-10 identical slides on 5-10 runs.


When changing Class II antibodies (ER, PR, Her2) and/or their detection 
systems you will need to run more extensive test. As Liz says, 40 positive, 
40 negative. Again, a tissue array is great for that.


Changing instruments these days is a big deal when a vendor ties you to 
their reagents.


Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala

Sent: Tuesday, February 08, 2011 3:20 PM
To: Weems, Joyce; Joe Nocito; Histonet
Subject: RE: [Histonet] IHC validation

Nope that's the recommendation for everything, in the paper they state
additional development is required for prognostic markers. Once you have
validated an antibody it only requires 3 tissues when you get a new lot
number:  1 strong positive, 1 weak to moderate positive and 1 negative.

From how I read the article its 25 tissues and then 3 tissues, it does

not talk about slides so it is possible to put multiple tissues on one
slide.  Again these are just recommendations; I do not think that there
is a set standard yet.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: Weems, Joyce [mailto:jwe...@sjha.org]
Sent: Tuesday, February 08, 2011 4:13 PM
To: Liz Chlipala; Joe Nocito; Histonet
Subject: RE: [Histonet] IHC validation

But that is for receptors, correct? Do you do that for everything?
Thanks, j

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Tuesday, February 08, 2011 17:58
To: Joe Nocito; Histonet
Subject: RE: [Histonet] IHC validation

Joe

If you are following the recommendations from the CAP paper on IHC
standardization then it would be 25 tissues (10 strong positive, 10 weak
to moderate positive and 5 negative).

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303)
682-9060 www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Tuesday, February 08, 2011 3:44 PM
To: Histonet
Subject: [Histonet] IHC validation

Greetings Histoland,
I need some help. We are about to switch IHC machines from the
Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per
antibody, do you run for the validation study? We have over 100 primary
antibodies. Normally, when we work up a new antibody, we  start with a
titer. Once that is established, we run 10 cases to check for
specificity. Hopefully we can obtain cases that are really positive,
some weakly positive and some flat out negative. Once that is completed,
we run 10 different tissue types to check for any unexpected
cross-reactivity.
   The ultra holds 30 slides and we are receiving two machines. If we
run 10 slides/antibody, that's going to take a while, not to mention the
number of detection kits that will be used. Do you think 5
slides/antibody is sufficient? I emailed CAP last week for their take
and they never returned my email (I told my medical director to hold
their check for the year and see how fast they respond

Re: [Histonet] High Complexity Testing

[Joe Nocito]

I was devastated to learn that IHC is not considered High Complexity 
Testing. I agree with Bonnie that we do not report results, therefore, IHC 
is not considered high complexity testing. Sad but true. Somebody pass the 
Scotch. I'm way past Tylenol


JTT
- Original Message - 
From: "Whitaker, Bonnie" 
To: "'Goins, Tresa'" ; "Horn, Hazel V" 
; "'Rene J Buesa'" ; 
; "Sheila Fonner" 

Sent: Tuesday, February 08, 2011 11:43 AM
Subject: RE: [Histonet] High Complexity Testing


Hi All,

There is a difference in performing a task (immunostaining) that is complex, 
and performing "high complexity testing" as the CLIA regulations govern.


Yes, staining is a complex task, and it requires knowlegable techs to ensure 
that it is properly done, and to troubleshoot difficulties when necessary.


It is "high complexity testing" because "testing personnel" in anatomic 
pathology are pathologists (and the non-physician people performing gross 
examinations, who must meet "high complexity testing personnel" 
requirements.


"Testing personnel" as defined by CLIA, are the people that report results 
of that test, not people who perform other related duties.


That's my explanation of the whole mess.

Bonnie Whitaker
AP Operations Director
Ohio State University Medical Center
Department of Pathology
614.293.5048


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa

Sent: Tuesday, February 08, 2011 12:22 PM
To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila 
Fonner

Subject: RE: [Histonet] High Complexity Testing

I must disagree with this assessment of what makes a test complex.  If the 
test is done properly [the responsibility of the technologist] then the 
reading to the test is a visual determination that requires experience on 
the part of the pathologist, but if the test is not done properly, will the 
pathologist be able to tell the technologist what to do to fix the problem?


Where's the Tylenol?


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel 
V

Sent: Tuesday, February 08, 2011 9:58 AM
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner
Subject: RE: [Histonet] High Complexity Testing

While the test is high complexity it is the READING of the test by the 
pathologist that determines its complexity.  Because histotechs do not 
report the results our part of this test is not high complexity.


Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa

Sent: Tuesday, February 08, 2011 10:32 AM
To: histonet@lists.utsouthwestern.edu; Sheila Fonner
Subject: Re: [Histonet] High Complexity Testing

When a "machine" is doing the test, there are stringent provisions as to the 
preparation and validations of the test.
Done manually, it requires a trained technologists and, yes, they are high 
complexity tests (both IHC and FISH, and their variations).

René J.

--- On Tue, 2/8/11, Sheila Fonner  wrote:


From: Sheila Fonner 
Subject: [Histonet] High Complexity Testing
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 8, 2011, 7:45 AM


Hello All,



I would really appreciate it if anyone has information on whether IHC/ISH 
are considered high complexity testing for histotechs. Our pathologist 
believes that ALL histology low complexity testing since a "machine" is 
doing the work. Can anyone help me out with some guidelines, literature, 
etc. that says otherwise? I would really appreciate it. We just want to know 
which one it is.




Thanks so much Histoland!







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[Histonet] IHC validation

[Joe Nocito]

Greetings Histoland,
I need some help. We are about to switch IHC machines from the Richard-Allen 
Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run 
for the validation study? We have over 100 primary antibodies. Normally, when 
we work up a new antibody, we  start with a titer. Once that is established, we 
run 10 cases to check for specificity. Hopefully we can obtain cases that are 
really positive, some weakly positive and some flat out negative. Once that is 
completed, we run 10 different tissue types to check for any unexpected 
cross-reactivity. 
The ultra holds 30 slides and we are receiving two machines. If we run 10 
slides/antibody, that's going to take a while, not to mention the number of 
detection kits that will be used. Do you think 5 slides/antibody is sufficient? 
I emailed CAP last week for their take and they never returned my email (I told 
my medical director to hold their check for the year and see how fast they 
respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are 
your thoughts? Thanks

Joe (JTT)


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[Histonet] Archives

[Joe Nocito]

Greetings all,
I'm going to show my ignorance (like that hasn't happened before). When 
accessing the archives, how do I do it? Do I search by topic or date? All the 
time I've been on here, I never had to use the archives. Thanks. Oh Oh, I feel 
another tear coming

Joe
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[Histonet] Consult fees

[Joe Nocito]

Just wanted to thank all of you for your responses. I may be a little 
sentimental and biased, but this is still a great forum. You all are great. I 
belong to another list server (which will left unnamed) and that "other" place 
doesn't even come close to the Histonet. Crap, I feel a tear or two coming on. 
Now I can't see the computer screen. Got to go and thanks again

Joe
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[Histonet] Consult Fees for IHC

[Joe Nocito]

Greetings histo land,
Hope everyone is having a great New Year. I have been asked to consult on IHC 
to a local firm. They asked me what my fees are. I told them we can talk about 
that later. I have no idea what to charge these people. I want to be fair, but 
I don't want to give this knowledge away (an y'all thought that this was just a 
petty face). Any ideas for the Texas area. This would be telephone and on site 
consulting for a research firm. Thanks in advance.

Joe
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Re: [Histonet] Hemoglobin A

[Joe Nocito]

thanks y'all for the info

Joe
- Original Message - 
From: "Feher, Stephen" 
To: "Joe Nocito" ; "Histonet" 


Sent: Monday, December 06, 2010 4:15 PM
Subject: RE: [Histonet] Hemoglobin A


We just got some from BioGenex order number is AR021-5R.


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Thursday, December 02, 2010 5:52 PM
To: Histonet
Subject: [Histonet] Hemoglobin A

Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any
more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc,
etc, etc, Biogenex. No luck. Thanks

Joe
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[Histonet] Hemoglobin A

[Joe Nocito]

Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any more and 
we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, 
Biogenex. No luck. Thanks

Joe
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Re: [Histonet] Current issues with Printmates and Checkmates

[Joe Nocito]

there is nothing to talk about. There was a problem, the company fixed it. End 
of story. I don't understand the fuss. But alas! I did receive another phone 
call at work today. Which prompted my supervisor to remind me that I must 
include a disclaimer. So here is my disclaimer

The expressed opinions and views in this transmission are those solely of the 
author. They are in now way, shape, or form representative views of the U.S. 
Air Force, U.S. government, the President of the United State, the Speaker of 
the House, the Minority Whip, my lawyers, their lawyers, your lawyers and their 
future off spring.

Unlike my other employment situation where I was on the verge of being fired 
(remember the good old days when I was forbidden from the Histonet?)  the 
current situation allows me to speak my mind, that is of course if I add the 
disclaimer. Then anything and everyone is open season. Which reminds me, those 
of you who live in a tourist town, how come when it's tourist season, you can't 
shoot them? There's deer season, bird season, crab season and tourist season. 
Just a thought.

- Original Message - 
From: "Mike Pence" 
To: "Joe Nocito" ; "Pamela Marcum" ; 
"Laurie Colbert" 
Cc: ; "Pamela Gholston" 

Sent: Thursday, November 18, 2010 11:33 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates


If things are as you stated you should want to talk with their upper
management.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Wednesday, November 17, 2010 5:54 PM
To: Pamela Marcum; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu; Pamela Gholston
Subject: Re: [Histonet] Current issues with Printmates and Checkmates


speaking of,
I guess my last posting rustled some feathers. I received a phone call
from 
my rep saying that upper management wants to talk to me. I told him that

there wasn't anything to talk about. Except that I was remiss. I want to

thank Chris for all her work in the lab. I'm sorry that I didn't mention

this before.
Any way back to the phone call- I told him that I said everything in my 
posting. No need to call me. At least this time I don't have to worry
about 
my CEO being called. He's in Washington D.C.
- Original Message - 
From: "Pamela Marcum" 
To: "Laurie Colbert" 
Cc: ; "Pamela Gholston" 

Sent: Wednesday, November 17, 2010 10:04 AM
Subject: Re: [Histonet] Current issues with Printmates and Checkmates




The ink also stays on through decal solutions which we needed. We do 30
bone 
marrows a day any system that could not stand up to decal was a no go
for 
us.



Pam Marcum

UAMS





- Original Message - 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?" Is the tape chemically resistant?

Histopathy


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- Original Message - 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot 

Re: [Histonet] Current issues with Printmates and Checkmates

[Joe Nocito]

speaking of,
I guess my last posting rustled some feathers. I received a phone call from 
my rep saying that upper management wants to talk to me. I told him that 
there wasn't anything to talk about. Except that I was remiss. I want to 
thank Chris for all her work in the lab. I'm sorry that I didn't mention 
this before.
Any way back to the phone call- I told him that I said everything in my 
posting. No need to call me. At least this time I don't have to worry about 
my CEO being called. He's in Washington D.C.
- Original Message - 
From: "Pamela Marcum" 

To: "Laurie Colbert" 
Cc: ; "Pamela Gholston" 


Sent: Wednesday, November 17, 2010 10:04 AM
Subject: Re: [Histonet] Current issues with Printmates and Checkmates




The ink also stays on through decal solutions which we needed. We do 30 bone 
marrows a day any system that could not stand up to decal was a no go for 
us.




Pam Marcum

UAMS





- Original Message - 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu

Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?"
Is the tape chemically resistant?

Histopathy


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- Original Message - 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu

Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?"
Is the tape chemically resistant?

Histopathy


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Re: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner)

[Joe Nocito]

we've had them for over two years and we still don't have the system up and 
running (for various reasons, some internal issues, some external issues). 
We had to stop automatically sending the slide queue from the cassette queue 
because of a lot of confusion with extra slides. A lot of slides were being 
wasted. We had to send the Checkmates back because the screens became dull, 
couldn't read the screen. They were fixed, I still don't know what the 
problem was. I even changed the battery on each machine. One time I called 
Thermofisher (or whatever they're called this week) because our cassette 
catcher wasn't working. I was told that they didn't have a manual for the 
Printmate and that it would cost $2000 to send out a rep to JUST look at it. 
I called my rep and asked what kind of crap that was. She told be to call 
the help desk, where I did get help and was able to fix it over the phone 
(thank you Cherryll).
Personally, I would not have purchased it. The Printmate has some quirks in 
it, even with the software up date. Sometimes, the machine looses it 
position. You have to close down the software, shut the machine off, turn 
the machine back on, wait for it to boot up, then open up the software. You 
have to use their cassettes because of the angle of the front of the 
cassette. We have tried other brands and only half the numbers were printing 
on the cassette. As some of you know, I really hate equipment that you have 
to USE their brand or it won't work. (remember my Ventana rants).
Wow, all that anger, frustration, just spilling out. not that I have anger 
issues. I've been to anger management classes 5 times.


Joe
- Original Message - 
From: "Damaris Beil" 

To: "histonet" 
Sent: Saturday, November 13, 2010 8:41 AM
Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide 
Mate (slide labeler with scanner)




Hello,

I'm interested in finding out if anyone is using ThermoFisher's  new 
PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and 
how they are working out for you.


Thanks in advance for your help,

Damaris
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Re: [Histonet] Cleaning molds

[Joe Nocito]

yeah, I'd be careful of this method. We used to do this at the U.S. Air 
Force hospital I used to work at when I was active duty. One time, the fire 
alarm went off and we had to evacuate the lab. Well, there must have been a 
real problem because we were out of the lab a long time. By the time we got 
the "all clear" to go back into the lab, I was the first one back and ran 
into some firemen. Seems the water boiled out of the pot and the paraffin 
was smoking up a storm.. No one said anything, but I bet that was why we 
were out of the lab so long. Now, those of you that have worked in a 
military or government hospital realize all the freaking paperwork that had 
to be filled out from that one incident. We were ordered by Safety to 
develop another method. So, we soak our molds in xylene for a couple of 
hours, covered of course as not expose the lab to the xylene fumes (that 
would required addition freaking paperwork) followed by a 100% etoh rinse, 
followed by air drying.


JTT
- Original Message - 
From: 
To: "Webb, Dorothy L" ; 
; 


Sent: Thursday, August 26, 2010 11:16 AM
Subject: Re: [Histonet] Cleaning molds


Back when I was using metal cassette lids and an autotechnicon (so no 
cleaning in the processor) I would throw all the lids and molds into a pot 
after embedding. Throw a bit of laboratory detergent in, fill with water, 
and boil on a hot plate. Once I got to a rolling boil, I would turn the 
heat off. By the end of the day it was cool.  Peel the paraffin off the 
top, then rinse the molds.


Worked great!  Didn't even need mold release.

Will

Sent from my Verizon Wireless BlackBerry

-Original Message-
From: "Webb, Dorothy L" 
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 26 Aug 2010 10:53:58
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cleaning molds

Does anyone in histoland clean their embedding molds in a dishwasher? 
Otherwise, besides placing in the cleaning cycle of your processor, how do 
sites clean their molds??  Simple, but plaguing question!!!  Thanks 
all!


Dorothy Webb




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[Histonet] Adenovirus controls

[Joe Nocito]

Greetings histoland,
does any one know of a company that sells adenovirus controls slides? I've 
tried NewComer's Supply, MasterTech, Biocare, Biogenex, ThermoFisherRichard 
Allen, or whatever they're called this week. Thanks

Joe
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Re: [Histonet] cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au

[Joe Nocito]

Happy Friday all,
try 10% sodium hydroxide for 1 hour prior to processing. It is important to 
let the slides drain really well before placing them in the oven. You want 
to make sure there is no water between the section and the slide. We use 
Plus slides with plain distilled water in our water baths. Hope this helps.

JTT
- Original Message - 
From: "Andrew Burgeson" 

To: 
Sent: Friday, August 13, 2010 3:57 PM
Subject: [Histonet] 
cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au




Joe "the toe" Nocito...are you out there?

Joe has good ideas about nails. Maybe he will send out his
procedure again.


I like using either potassium hydroxide 10-20% or Sodium
hydroxide 10-20% for softening nail fragments before
processing.

Also, keep in mind that soft tissues attached are equally as
important and sections of nail beds need to be of high
quality. A melanoma under a nail can be a bad situation.
Sometimes in addition to PAS or GMS stains for
onychomycosis, we have done melanin and iron stains for
areas of pigment or hemmorhagic depositions.

Joe...are you out there? lol

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Re: [Histonet] Just three months?????

[Joe Nocito]

I see that now, wasn't the first time I was wrong today, probably wouldn't 
be the last either


- Original Message - 
From: "Jeffrey Silverman" 

To: 
Sent: Wednesday, March 31, 2010 5:03 PM
Subject: [Histonet] Just three months?


CAP is now saying no more gross processing of small things that are entirely 
submitted- it's all gross examination now whether we mean straining 
currettings into a cassette or dissecting a complex cancer resection. Dumb 
as all get out if you ask me.


As for the three month training thing, Joe, I'm not so sure about that. They 
seem to spell out specific amounts of college education required IN ADDITION 
to training in the laboratory.

The requirement first spells out two education choices-
1: an earned associates degree in medical laboratory science

OR 2:Education/training equivalent to the above that includes at least 60
semester
hours or equivalent from an accredited institution. This education
MUST (caps mine) include 24 semester hours of medical laboratory technology 
courses, OR 24

semester hours of science courses that includes 6 semester hours of
chemistry,
6 semester hours of biology, and 12 semester hours of chemistry,
biology
or medical laboratory technology in any combination.

So in addition to all the college courses, which we all know you need to 
count and measure six endoscopic biopsies and put them in a cassette, much 
more training and experience needed than what an unregistered on the job 
tech needs to orient and embed them properly LOL!!! CLIA then requires that 
the
individual must have (additional) laboratory training including either 
completion of

a clinical laboratory training program approved or accredited by
the ABHES, NAACLA, or other organization approved by HHS (note that
this training may be included in the 60 semester hours listed
above),
OR at least 3 months documented laboratory training in each
specialty in which the individual performs high complexity testing.

Now there are grandfathering clauses in CLIA which may enable folks (like 
myself) to continue to gross. I haven't digested that yet, but I'm ready to 
get that job in Pathmark if necessary.


Sheesh, does it ever end?

Jeff Silverman
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Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

[Joe Nocito]

I've seen lab get accredited one month, only to have CLIA come through the 
next month and rip that lab apart. I have been through CAP inspections that 
consisted of the inspector looking only at slides while he checked off the 
checklist to inspectors carrying their own thermometers and testing the temp 
of the paraffin baths on the tissue processors. This is supposed to be a 
peer review, often it's a witch hunt. I had one CAP team leader tell me that 
my lab was the last lab he was going to inspect because he dropped out of 
CAP and went with JCAHO, which was approved by his hospital administrator.
Does anyone know why CAP split the "processing" and "grossing parts anyway? 
I thought it was a stupid idea in the first place. But then again, I've 
never been known to be politically correct


Joe
- Original Message - 
From: "Andrew Burgeson" 

To: 
Sent: Thursday, April 01, 2010 12:26 PM
Subject: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com



Sheesh is right, J.

CAP is all politics as far as I am concerned. It is all
about protecting the careers and paychecks of the general
pathology community.

I am thouroughly unimpressed with JCAHO, CAP et al.

If all you need to legally run a laboratory is to be CLIA
inspected, then WHY BOTHER with these subjective entities?


The BS I have heard over the last few months concerning MOHS
surgery specimens is one glaring example of the limitations
CAP has in understanding fully certain nuances of the lab
trade.

Ridiculous. Unless you want the marketing and potential
"perception" that you are better covered from a legal
standpoint, CAP certs are worthless.

The more I hear about CAP certifications, the more I see it
as a certain community of individuals who are protecting
their perceived "TURF."

In the end, the pathologists in the group and in the
facility in which you are working have to take
responsibility for these matters. If the docs think a CAP
cert is necessary, then do it and live with it. If not, then
consider yourself lucky to not have to see these people in
your lab.

I have been through MANY CAP inspections in and out of the
military. For the most part, though, I see people paying
this organization to inspect their lab as the same thing as
"burning a pinch of incense in honor of great Caesar, ruler
of Rome." It will get you some kudos, but tangibly not
change much at all if your pathologists or HR $ hiring hands
want to pocket more $ as a result of hiring "pregnant out of
wedlock 16 year olds" to gross tissue and cut slides.

Seen it.

AB

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Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines

[Joe Nocito]

just had a lively discussion at work. My take is that the only thing CAP 
changed was that they combined the "processing" and "grossing" pieces 
together again, which I don't know why they split them in the first place. 
But you don't have the entire CAP note and many people miss this.  The last 
item states OR three months of documented laboratory training in the high 
complexity area. Again, my take is that an unregistered histotech can have 
at least three months of documented training in grossing complex specimens, 
have the record signed off by the medical director and be ok. How far off am 
I?


Joe
- Original Message - 
From: "Mahoney,Janice A" 

To: "Histonet" 
Sent: Wednesday, March 31, 2010 2:44 PM
Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines


Is anyone concerned about the new (old) grossing personnel guidelines from 
CAP.  Many labs use people to "process " tissue.  No more!



ANP.11610 Phase II

If individuals other than a pathologist or pathology resident assist in 
gross examinations, do such individuals qualify as high complexity testing 
personnel under CLIA regulations?


NOTE: The laboratory director may delegate the dissection of specimens to 
non-pathologist individuals; these individuals must be qualified as high 
complexity testing personnel under CLIA regulations. The minimum 
training/experience required of such personnel is:


1.  An earned associate degree in a laboratory science or medical 
laboratory technology, obtained from an accredited institution, OR
2.  Education/training equivalent to the above that includes at least 60 
semester hours or equivalent from an accredited institution. This education 
must include 24 semester hours of medical laboratory technology courses, OR 
24 semester hours of science courses that includes 6 semester hours of 
chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, 
biology or medical laboratory technology in any combination. In addition, 
the individual must have laboratory training including either completion of 
a clinical laboratory training program approved or accredited by the ABHES, 
NAACLA, or other organization approved by HHS (note that this training may 
be included in the 60 semester hours listed above), OR at least 3 months 
documented laboratory training in each specialty in which the individual 
performs high complexity testing.


The CLIA regulations on high complexity testing personnel may be found at HC 
Testing Personnel.


In addition, the CLIA regulations include exceptions for grandfathered 
individuals; these regulations (42CFR493.1489 and 1491) may be found at the 
above Web address and at Grandfathered 
Exceptions.


It is the responsibility of the laboratory director to determine whether an 
individual's education, training and experience satisfies the requirements 
of this checklist question.

Jan Mahoney
Omaha, NE


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Re: [Histonet] Alcohol Source?

[Joe Nocito]

depends. A fine Scotch whiskey may be aged in old oak casks, while a 
delicate Tequila may not be
- Original Message - 
From: "Breeden, Sara" 

To: "histonet" 
Sent: Tuesday, March 02, 2010 10:44 AM
Subject: [Histonet] Alcohol Source?


I hate to even ADMIT this and I ought to use a disguise when asking -
but is alcohol a wood-based solution?  I should know this - I know...



Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576



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Re: [Histonet] Fire in the lab

[Joe Nocito]

Once upon a time in a far away land, we used to boil our embedding molds in 
boiling soapy water, over an open Bunsen burner, followed by an alcohol 
rinse then air dry. One time the fire alarm was activated and we had to 
evacuate the hospital. We were out there quit awhile. When we received the 
all clear to go back into the hospital, I was the first one back in the lab 
and the fire department was there, looking into our pot that had boiled out 
and was smoking up the lab. This wasn't the cause of the first alarm, but it 
did set off the second.


Joe
- Original Message - 
From: "CHRISTIE GOWAN" 

To: 
Sent: Friday, February 26, 2010 8:20 AM
Subject: [Histonet] Fire in the lab





Dear Histonet Friends,

I just wanted to share an incident we recently had with an old paraffin pot. 
One of my techs came in on Sunday to embed some tissues, went into the 
processor room and smelled something burning. He noticed our old paraffin 
pot had charred looking labels on the outside so he went over, opened the 
lid and poof!!! the pot went up in flames. The thermostat had gone haywire 
and heated the paraffin to flash point. Opening the lid gave it the oxygen 
it needed to ignite. He triggered the alarm, made the appropriate call and 
then put it out with an extinguisher. Of course it kept re-igniting because 
he could not get behind it to pull the plug. The fire dept finally was able 
to get it pulled out and unplugged. Needless to say the tech was shaken and 
the room was a mess. I applaud his courage and am not sure I would have done 
the same. There was enough xylene and alcohol on the 4 processors to cause 
quite an explosion but everything else was in a flammable cabinet. I was 
wondering if this type of thing had ever happened to anyone else?? Needless 
to say, we have de-comissioned all old paraffin pots and will order only 
those with over temp safety features. I guess I just wanted to remind 
everyone that fires can happen in the lab and do probably more often than we 
hear about. This was the first time for me and I have been in this business 
for over 20 years. Take care and be safe.


Christie Gowan HT (ASCP)
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Re: [Histonet] Crystal/Mount

[Joe Nocito]

Thermo Fisher or whatever they are called this week has it

Joe
- Original Message - 
From: "Rene J Buesa" 
To: ; " Ruth A (NIH/NIDCR) [E]Yaskovich" 


Sent: Friday, February 26, 2010 2:28 PM
Subject: Re: [Histonet] Crystal/Mount


Why you just don't Google it?
René J.

--- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] 
 wrote:



From: Yaskovich, Ruth A (NIH/NIDCR) [E] 
Subject: [Histonet] Crystal/Mount
To: "histonet@lists.utsouthwestern.edu" 
Date: Friday, February 26, 2010, 3:10 PM


I know this has been asked before. Where can I purchase Crystal/Mount 
mounting media?

Thanks
Ruth N.I.H.
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Re: [Histonet] Problems with Denatured Alcohol?

[Joe Nocito]

Pam is correct, I mean, look at me, I've been denatured for many years.
Happy New Year everyone

JTT
- Original Message - 
From: "Pamela Marcum" 

To: "Smith Wanda" 
Cc: 
Sent: Monday, January 04, 2010 9:52 AM
Subject: Re: [Histonet] Problems with Denatured Alcohol?




Wanda,



The first thing you need to find out is what did they denature it with. 
Denatured alcohol may not be the mixture of isopropanol and methanol in 
ethanol you were getting and could be chemically denatured.




This is a problem that comes up when we don't check all the variables in 
what is actually in the products we buy and the vendor doesn't tell us why 
it got cheaper. If it is a chemical denaturing of the alcohol it could be 
designed for use in one of many fields just not histology. The real 
requirement for reagent and denatured alcohol with the ATF is that the 
product be undrinkable or unfit for human consumption. Also different 
manufacturers use varying amounts of isopropanol/methanol/ethanol and that 
can change the way it processes and stains tissue. The best is 5% each of 
isopropanol and methanol with the remaining 90% ethanol.




Pam Marcum

UAMS


- Original Message - 
From: "Smith Wanda" 

To: histonet@lists.utsouthwestern.edu
Sent: Monday, January 4, 2010 9:25:08 AM GMT -06:00 US/Canada Central
Subject: [Histonet] Problems with Denatured Alcohol?

Happy New Year to Everyone!
We recently changed from Reagent Alcohol to Denatured Alcohol as a cost 
saving measure per the "bean counters". The cutting histotechs have started 
complaining that the tissue seems dry and they are having difficulty cutting 
sections. Has anyone else had this problem and are there any adjustments in 
processing times, etc that may help the situation???

Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax


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Re: [Histonet] Nail softner

[Joe Nocito]

20% sodium hydroxide works great. Hey, they don't call me "Joe the Toe" for 
nothing. Happy New Year


JTT
- Original Message - 
From: "DELIA GARCIA" 

To: 
Sent: Thursday, December 31, 2009 1:26 PM
Subject: [Histonet] Nail softner


Hi Everyone,

I have a question... What is a good nail softner to use at the grossing 
table prior to fixation? We were currently using KOHw/DMSO from Healthlink 
Healthcare. However I cannot find who sells it. Healthlink only distributes. 
Plus I dont have the time to keep hunting for it. I'm hoping there are other 
alternatives you all might be using that you can share with me. What we had 
has lasted us my whole time here and then some so I havent had to order it 
before. Any suggestions would be so greatly appreciated. Thank you in 
advance. You all have a Happy New Year


Delia
Lead Histologist



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Re: [Histonet] looking for KOH nail procedure

[Joe Nocito]

we use 20% sodium hydroxide. Works great
- Original Message - 
From: "Cheryl" 

To: 
Sent: Wednesday, December 16, 2009 4:08 PM
Subject: [Histonet] looking for KOH nail procedure


Hi Guys!

I've used a potassium hydroxide solution to 'soften' nails after fixation 
but before processing. I cannot find my procedure!!


Can anyone help?

Cheryl Kerry, HT(ASCP)

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Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports

[Joe Nocito]

I have a question similar to this one.
If I take 2 IVD antibodies say cytokeratin AE1/AE3 and 34BF12 and make these 
into one cytokeratin cocktail, is this considered an ASR because I combined 
them into another antibody?


Joe
- Original Message - 
From: "Morken, Tim" 

To: "Foshey, Annette" ; 
Sent: Friday, November 20, 2009 12:24 PM
Subject: RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology 
reports



The disclaimer is only for ASR antibodies. They don't have to be labeled 
"experimental" because a CLIA certified lab has full capability and 
authority to validate any antibody they want to use for any purpose. You do 
have to document your validation procedure and results.


You can also use RUO antibodies under CLIA regs as long as you do the full 
documented validation. Interestingly there is no suggested disclaimer for 
RUO antibodies.


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, 
Annette

Sent: Friday, November 20, 2009 9:55 AM
To: (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology 
reports


What is the current practice for meeting this regulation?  Is a general 
statement (disclaimer) placed on all pathology reports that have 
immunocytochemistry and/or
FISH and ISH or is the disclaimer placed only on the reports where the class 
1 ASR antibody or nucleic acid have been used?


Thanks for your input?
Annette Foshey
Charge Tech in Histology
Children's Hospital of Wisconsin
414-266-6580Fax 414-266-2779
afos...@chw.org


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Re: [Histonet] Lumpectomy question

[Joe Nocito]

Debbie,
as far as I know, there is no modifier, all you can charge is a 88307. 
That's the same case when you have a skin excision at a 88305. Even if it's 
a melanoma excision and it takes 20 blocks, you can only charge an 88305. 
The system is FAR from perfect.


JTT
- Original Message - 
From: 

To: 
Sent: Friday, November 20, 2009 1:17 PM
Subject: [Histonet] Lumpectomy question



Good Friday Afternoon,
Does anyone know if there is a modifier for 88307 for Lumpectomies when
20-30 blocks are submitted.
We are currently charging 88307 but the pathologist thinks there should be
a modifier.
Thanks.

Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical
Center I
200 Medical Park Boulevard I Petersburg, Va.  23805 I T: 804-765-5050 I F:
804-765-5582 I dkb...@chs.net





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Re: [Histonet] Oscar

[Joe Nocito]

Oscar? when I was the supervisor of the immuno lab at the AFIP, our rabbit 
that produced our anti-keratin died.  The civilian researcher and I were 
working up multiple cytokeratin cocktails to replace Fluffy. We wanted to 
call it the BOZO antibody, but our medical director refused. I can see it 
now, in print " 50 cases were stained with the Bozo antibody", now there's 
Oscar. Tell me this antibody didn't come from Hollywood.


JTT
- Original Message - 
From: "Maria Katleba" 
To: "Patti Loykasek" ; "histonet" 


Sent: Monday, September 28, 2009 3:33 PM
Subject: RE: [Histonet] Oscar


Patti,

Thank you for taking time to talk to me. I appreciate the help!

Kind regards,

Maria Katleba HT(ASCP) MS
Queen of the Valley Medical Center
Napa CA 94558

707-257-4076


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti 
Loykasek

Sent: Monday, September 28, 2009 1:28 PM
To: histonet
Subject: [Histonet] Oscar

Hi All. I understand that there was a recent question regarding the antibody
Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We
routinely work up Oscar using normal liver. There should be staining of the
bile ducts and the hepatocytes. I'd be happy to offer more info if the
person who originally posted will contact me. I'll try not to take vacation
again ( at least until the next available time!). Happy Monday.

Patti Loykasek
PhenoPath Laboratories



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Re: [Histonet] GMS-Fungus on nails

[Joe Nocito]

We soak our nails in 20% sodium hydroxide for at least an hour, rinse in 
running tap water for a few minutes before  we place the cassettes on the 
tissue processor. We use positive charged slides, heat slides in 80 degree 
oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot 
more gentler on the tissue that the GMS. We are the toenail lab for the U.S. 
Air Force ,


Joe "The Toe"

- Original Message - 
From: "jstaruk" 
To: "'Clare Thornton'" ; 


Sent: Friday, July 24, 2009 10:41 AM
Subject: RE: [Histonet] GMS-Fungus on nails



Prepare a 10% solution of Titebond II premium wood glue (found in most
hardware stores).  Dip the slide in the solution just before mounting the
section on the slide.  Let the slide dry and stain away.

Jim

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  www.nehorselabs.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Friday, July 24, 2009 11:31 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] GMS-Fungus on nails

We do a GMS for fungus on all nails we receive.  We often have a difficult
time keeping the nail tissue on the slide.  We've tried baking for long
periods, pre-treating in formalin, using silane slides, with no luck. 
Even

when the nails cut relatively easily we still lose it, and end up running
several GMS stains before we might get a speck or two of tissue we can 
look

at.  We use the Artisan stainer for our specials.  We are really not
interested in performing the GMS manually, due to volume and turn around
time restrictions.  Any suggestions?

Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com

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Re: [Histonet] What percent of HTL's do not have a BS degree?

[Joe Nocito]

I wouldn't say that histology is a dead end job, I mean, I jumped to a PA 
and I know that I couldn't have passed that exam without my histology 
experience. The idea of adding an HTL category was initially a good one. 
Trying to bring us up to the MT and CT levels, but honestly, I think it fell 
short. Even after I received my BS, people asked me if I was going for my 
HTL. I told them no because I saw no benefit from it. At that time, I felt 
that if I couldn't get hired as a supervisor with a BS and 20 years 
experience, with 12 as a supervisor, I wasn't going to get the job any way.
I'm not degrading HTLs by a long shot. I'm just saying for me, it wasn't 
worth it. After I retired from the Air Force, I was directly hired as a 
supervisor without the HTL. Just my three cents (inflation you know)


JTT
- Original Message - 
From: 

To: "Michael Bradley" 
Cc: 
Sent: Tuesday, July 14, 2009 4:18 PM
Subject: Re: [Histonet] What percent of HTL's do not have a BS degree?




Mike,



I couldn't agree with you more. I'm in the same position as you. In the 
Boston, Mass. area people are taken right off the street and work for a year 
as a lab assistant then promoted to a tech in training. Most have a hs 
diploma, no ambition and expect good pay for bad work and poor work ethic. I 
have been in the histology field for 20 years and don't consider it a 
profession or a career, just a job.




Ron Martin, BS, HTL (ASCP)


- Original Message - 
From: "Michael Bradley" 

To: "Joyce Weems" 
Cc: histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern
Subject: Re: [Histonet] What percent of HTL's do not have a BS degree?

HI all

I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the
early 90s and I guess I was misguided because I thought it would open more
doors for me than just an HT. I was sadly mistaken. After I passed my test
I waited 9 months for a raise and promotion (which was just a greater title)
and when I got my raise so did 2 other employees that didn't even have or
try for their certification. I spent many nights and weekends studying and
doing my stains for the test. I am proud of my accomplishments. It is a
shame that our industry does not reconize the difference between HT and
HTL. A few years back I was working as a traveling histotech and when I
tried to get a permanent position no one wanted to hire me because I was
over qualified by having over 15 years experience and a HTL certification.
I worked hard to no avail. The histology world doesn't look for well
qualified workers they look for cheap labor. I have heard more than one
pathologist state that "a monkey can do our job." I have also worked in a
lab where they would hire someone with a GED to cut slides. A career in
histology is for the most part a dead end and there is no future. As long
as our industry doesn't respect education and experience there will be less
and less histotechs and the quality of the slides will suffer which in turn
will bring down patient care.
Just my 2 cents.

MB proud HTL
On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce  wrote:



Honey... You are a mere child! There are some of us that have been in
the business for 40+ years. I missed the grandfather approach by 7 mo -
time that I didn't work moving from place to place with my military
ex-husband.

But I did finally get the degree and do the exam. But we're still
around. And I'll probably be working till I'm 100!!! J:>)


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas
Jasper
Sent: Tuesday, July 14, 2009 15:16
To: Feher, Stephen
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] What percent of HTL's do not have a BS degree?

Hi Steve,

I've got no statistics to offer you...just an observation. I would say
that finding an HTL, without a Bachelor's degree is akin to the
proverbial needle in a haystack. Anyone that obtained their HTL,
if/when they could be grandfathered in, is likely to be retired or close
to it. First of all, most folks that went the OJT route for
certification were eligible to sit for the HT only (to my knowledge).
I've never met anyone with an HTL that did not have a Bachelor's as a
pre-requisite. I've been doing histology for ~25 years. I've met
people from all over the country and various parts of the world. Truth
is there isn't an abundance of HTLs out there. Unlike the Medical Lab
world, with the basic differences between MTs and MLTs, anatomic path
does not exactly mirror that with the HTL and HT. It's true the MT and
HTL both require a Bachelor's, but responsibilities in most labs, etc.,
generally do not hinge on someone being an HT vs. an HTL.

A person like myself is probably more common (Bachelor's and an HT).
Unless you know of someone in particular; that you want to hire, with an
HTL without a Bachelor's, I wouldn't waste time trying to justify it. I
guess 

Re: [Histonet] misleading message on Histonet last Friday

[Joe Nocito]

let's stop monkeying around OK? (couldn't resist)

JTT


- Original Message - 
From: "Maria Katleba" 
To: "LINDA MARGRAF" ; 


Sent: Monday, July 13, 2009 1:48 PM
Subject: RE: [Histonet] misleading message on Histonet last Friday


OMG... I do not like primates (especially gorillas)... So I probably won't 
be buying anything from them any time soon.  Just kidding. Seriously, I 
probably would stay away from that kind of a marketing name unless you are 
appealing to primate research facilities.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA 
MARGRAF

Sent: Monday, July 13, 2009 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] misleading message on Histonet last Friday

Dear Histonetters:
I got the message below privately from one of the list members and (with 
their permission) wanted to pass it along to the membership.


Dear Linda:
Matthew Semovoski [histosea...@gmail.com] posted this on Histonet this AM:
Hey everyone,
I am looking at getting some supplies for a new histology lab. Has anyone 
ever purchased from Gorilla Scientific? www.GorillaScientific.com  I just 
got a flyer in the mail from them and it seems like they have some really 
good prices. I think I can save a lot on my slides if I purchase from them.

Has anyone else on here purchased anything from them?
Thanks,
Alex
histosea...@gmail.com
Well it was not Alex who posted it was Matthew Semovoski who posted this & 
he is the man who answers the phone when you call up Gorilla Scientific. I 
as a sales person play fair & do not try to do any slick sales adds on 
histonet & wanted to make you aware of this. (I really am such a geek & LOVE 
TO LEARN from Histonet too!) So I really do want to thank you for all of the 
hard work that you do & just wanted to make you aware of this instance.

Thank you so much & have a great weekend!


I don't want to turn this into a major discussion but would like to remind 
everyone that we run Histonet using University of Texas resources and they 
would not appreciate (or support) its use for commercial advertising even if 
it is disguised in a misleading message.

Thank you,
Linda M
Histonet administrator



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Re: [Histonet] Grossing techniques

[Joe Nocito]

 a.. Manual of Surgical Pathology by Susan Lester
 b..
 c.. Publisher: Saunders W B Co
 d.. Pub. Date: October 2005
 e.. ISBN-13: 9780443066450
 f.. Sales Rank: 56,744
 g.. 384pp
 h.. Edition Description: REV
 i.. Edition Number: 2
We use this one. It has good pictures with samples of gross descriptions.  I 
wish I knew about this book before I took my PA exam.

Joe Nocito, BS, PA, HT (ASCP)QIHC
San Antonio, TX
- Original Message - 
From: "Karin Groeger" 

To: "Jaime Plata" ; 
Sent: Friday, June 12, 2009 3:10 PM
Subject: RE: [Histonet] Grossing techniques


Surgical Pathology Dissection  (Second Edition) Written by William Westra, 
Ralph Hruban, Timothy Phelps and Christian Isacson  a good guide with a lot 
of illustrations.


Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaime Plata

Sent: Friday, June 12, 2009 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Grossing techniques

Hello Fellows, Anybody knows or recommend a book or manual for grossing 
tissue techniques ? please let me know. I am looking for a more practical 
one on  gross description. Thanks you all

Jaime E Plata
MT.HTL (ASCP)
enrri...@yahoo.com



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[Histonet] archive help

[Joe Nocito]

Greetings all,
I'm going to admit that I'm a dummy and need help with getting info from the 
archives. I'm specifically looking for postings dealing with using freeze spray 
while doing frozens. So, how do I get to the archives again? That sucking noise 
is me pulling my head out of my butt. Thanks

JTT
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Re: [Histonet] Grossing

[Joe Nocito]

Schaundra,
we have residents and  2 PAs perform all the grossing. In real tight 
situations, we have a couple of qualified grossing histotechs we can rely on 
in a pinch. we have histotechs set up the specimens, but the residents and 
PA's gross alone. We are responsible for annotating any special procedures 
that need to be ordered. Our grossing cutoff time is 5:30 PM because of the 
start time of the tissue processors (we start embedding at 3:00 AM).


JTT
- Original Message - 
From: "Schaundra Walton" 

To: "Histonet" 
Sent: Thursday, April 23, 2009 1:25 PM
Subject: [Histonet] Grossing


We are evaluating some of our grossing procedures and cut off times and I 
was wondering what other people are doing.


Who does your grossing, pathologist, PA, or other qualified individuals?
Who does you accessioning, histotechs, support staff, or other?
What is your cut off time for grossing tissues?

Right now our pathologists gross all tissues, the histotechs accession, and 
grossing is done up until 6pm (which is when the processor begins).


Thanks for the info!

-Schaundra Walton HTL(ASCP)
Histology Supervisor
Swedish American Hospital
Rockford, IL



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Re: [Histonet] commercial control slides

[Joe Nocito]

this is hard to do because of the frozen section requirement. However, we 
make smears out of mayonnaise when we have Oil Red O stain and it works 
well. Good luck


JTT
- Original Message - 
From: "Steven Joy" 

To: 
Sent: Thursday, April 23, 2009 1:38 PM
Subject: [Histonet] commercial control slides


Can anyone recommend a brand or line of commercial control slides for oil 
red O?


Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven@capitalhealth.ca

This communication is intended for the sole use of the recipient to which it 
was addressed and may contain confidential, personal or privileged 
information. Please contact the sender immediately if you are not the 
intended recipient of this information and do not copy, distribute or take 
action relying on it. Any communication received in error, or subsequent 
reply, should be deleted or destroyed. Thank you.


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Re: [Histonet] Gross Photography

[Joe Nocito]

In government facilities, we are now banned from using flash drives, memory 
sticks, and other portable devices because some knucklehead at some military 
installation downloaded a nasty worm that affected many military computers 
(glad I wasn't that person, probably digging latrines in Iraq or Afghanistan 
now). This puts us in a tough spot because I was able to shoot pictures in 
the grossing room. them emailing them to the sign out pathologist. Many 
times, the path I'm grossing for is out for one reason or another and it 
helped them see the specimen before I laid blade to specimen. Now, we have 
to wait for the path to come to the grossing area or put the specimen aside 
until they can come by.


JTT
- Original Message - 
From: "Michael Mihalik" 
To: "'kemlo'" ; "'Sate Hamza'" ; 


Sent: Saturday, April 18, 2009 7:46 AM
Subject: RE: [Histonet] Gross Photography



Just as another endorsement for this practice.  Digital images seem so
important to us that in our information system, a hyperlink to all images 
is

included in case query.  Hence, you can see the image at the same time
you're reading all the other details of the case.

It's just one more piece of information that helps provide a better
diagnosis.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kemlo
Sent: Saturday, April 18, 2009 3:01 AM
To: 'Sate Hamza'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Gross Photography

As a Biomedical Scientist I agree with you totally. One of the weaknesses 
of

Biomedical scientists performing the 'grossing' is that the original
evidence at dissection is lost to the Pathologist (that is until that Time
Biomedical Scientists carry out the interpretation). Taking digital photos
at all stages of dissection retains the evidence for the reporting
Pathologist.

I did this for many years when dissecting samples for my Pathologist; 
saved
drawing diagrams. I guess you'd agree that 80% of all interpretations 
could

be carried out by a Biomedical Scientist (Histotech) once competency is
attained and the envelope of responsibility is agreed. It's happened in
Cytopathology in the UK!

Kemlo Rogerson MSc MIBiol CBiol CSci DMS FIBMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sate Hamza
Sent: 18 April 2009 06:37
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Gross Photography

As a pathologist, I am a strong proponent of ample gross photography in 
the

cutting room. When I first started in my current place, I thought that not
much gross photography was being done. This has increased in recent years 
in

our center. I always encourage our residents to take digital gross
photographs. I recently bought an easy to use digital camera and gave it 
as

a gift to our cutting room to encourage more digital photographs.

I think that the availability of easy to use digital cameras has made 
taking

pictures much easier. A picture is a great tool for documentation and for
communication. No matter how skillful and expressive the gross description
is, a picture can make things much easier for sign out. If sections need 
to

be mapped for margins or other considerations, one can take a digital
picture, make a quick print out of it and map the sections on it. Such
pictures are so helpful, for example, for excisions of flat pigmented
lesions from sun-damaged skin. Gross-microscopic correlation can help so
much in assessing margin status (this can be so difficult with microscopy
alone). It also helps in excisions of vulvar lesions/tumors and in
irregularly shaped complex excisions from any site.

The digital photos can be taken quickly. They do not need to be textbook
quality. The goal usually is to facilitate communication and facilitate 
the

sign-out process.

The pictures that our PAs take are placed on a network server in folders
that are named with the accession number. The printouts with sections 
mapped

are kept in a binder in the cutting room where a pathologist can find them
when the need arises.

Sate

On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito  wrote:


like Mike,
we only photograph unusual specimens. Seems photographing specimens has
become less and less important. Kind of like autopsies.





--
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada
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Re: [Histonet] Gross Photography

[Joe Nocito]

like Mike,
we only photograph unusual specimens. Seems photographing specimens has 
become less and less important. Kind of like autopsies.


JTT
- Original Message - 
From: "Mike Pence" 
To: "Steven Joy" ; 


Sent: Friday, April 17, 2009 4:20 PM
Subject: RE: [Histonet] Gross Photography


I only photograph specimens that are not "routine" type specimens.
Something that you might see only a few times a year or that once in a
lifetime specimen. We also will get request from the surgeon to
photograph a specimen for them at gross.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven
Joy
Sent: Friday, April 17, 2009 4:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gross Photography


Is there a range of practice among centers as to what specimens are
photographed at gross, does anyone photograph pretty much all specimens?
Steve Joy, BSc. MLT Research and Development Technologist 5B2.03
Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton
Ab T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven@capitalhealth.ca

This communication is intended for the sole use of the recipient to
which it was addressed and may contain confidential, personal or
privileged information. Please contact the sender immediately if you are
not the intended recipient of this information and do not copy,
distribute or take action relying on it. Any communication received in
error, or subsequent reply, should be deleted or destroyed. Thank you.

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Re: [Histonet] Controls needed!

[Joe Nocito]

I second that. Marsha is the loveliest person I know.
- Original Message - 
From: "Denise Piontek" 

To: 
Sent: Wednesday, April 08, 2009 8:49 PM
Subject: RE: [Histonet] Controls needed!



Newcomer Supply has been a reliable control source, when necessary the have 
"customized orders".



Date: Wed, 8 Apr 2009 07:14:21 -0500
From: jennifer.l.hofec...@vanderbilt.edu
To: dknut...@primecare.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls needed!
CC:

Hi Deanne,
Have you checked with the NSH Control Tissue Bank? The form to request
tissue is online on the NSH web page (www.nsh.org). The bank is run by
the Quality Control committee in conjunction with the IHCRG. It is a
free service to NSH members.
You may contact the committee chair, William DeSalvo at
wdesalvo@hotmail.com with any specific questions.
Have a great week.

Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN
ph 615.343.0083
fax 615.343.7089
-Original Message-
From: Knutson, Deanne [mailto:dknut...@primecare.org]
Sent: Tuesday, April 07, 2009 3:06 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Controls needed!

We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We
have
good GRAM control blocks that we would be happy to exchange. Please let
me
know if you can help us out. Thank you!



Deanne Knutson

Anatomic Pathology Supervisor

St. Alexius Medical Center

Bismarck, N. Dak. 58506

701-530-6730

Fax 701-530-6735



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Re: [Histonet] Negative IHC controls

[Joe Nocito]

we're running one negative normal serum slide per block. For example, if 
we're performing mouse antibodies i.e. CD-45, HMB-45, pan Cytokeratin, we 
run one IgG mouse normal serum control. If we are running rabbit polyclonals 
(or rabbit monoclonals) and mouse monoclonals on the same case, we usually 
would run a IgG normal mouse and normal rabbit serum slide. Now, I know the 
purists out there are going to tell me what if I use an IgM mouse 
monoclonal, I should be running an IgM mouse normal serum. I agree, but my 
budget doesn't allow to run a negative for all scenarios. In that frame of 
mind, if we run a case with multiple different pretreatments, we run a 
negative on the harshest pretreatment. Again, not ideal, but I think it is 
better than just putting PBS or TBS buffer on a slide and calling that 
negative. That's my 3 cents worth and I'm sticking to that story.


JTT
- Original Message - 
From: "Sheila Haas" 

To: 
Sent: Wednesday, April 08, 2009 1:57 PM
Subject: [Histonet] Negative IHC controls


I have a question concerning ANP.22570 on the CAP checklist. Could you all 
tell me how you are handling
negative controls for IHC staining? The question actually states (and I've 
confirmed with CAP) that we should be running two types of negative 
controls. One for reagents and one for each antibody in a run. I'd like to 
know
what the practice is. This seems very costly and time consuming. Thanks in 
advance!


Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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Re: [Histonet] Benchmark XT

[Joe Nocito]

another issue could be the mixer blowing too hard. I've had that happen too.

JTT
- Original Message - 
From: "Kari Bradshaw" 
To: "Dana Spears" ; ; 
"Phyllis Thaxton" 

Sent: Wednesday, April 08, 2009 2:13 PM
Subject: RE: [Histonet] Benchmark XT


We just recently went through a similar problem with irregular staining,
light staining, sometimes no stain of any kind including
counterstain,tissue falling off, and a handfull of false negative
patient tissues, but positive controls (on the same slide). With two
XT's running continuously it taken several days and everyone's help
isolating the problemnot to mention several different lots numbers
of slidesyikes! Next time I'll blame the slides right off the bat.

Kari L. Bradshaw HT(ASCP)
Laboratory Manager
Lower Columbia Pathologists
(360)425-5620
kbrads...@lcpath.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana
Spears
Sent: Wednesday, April 08, 2009 9:24 AM
To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton
Subject: Re: [Histonet] Benchmark XT

I would definitely check your slides - we have found that when we get a
bad lot of charged slides, things start washing off on the XT -
regardless of detection, antibodies, whatever.  It was happening to us
on some tissue types and not on others, but a new batch of charged
slides did the trick.

I've never had to dry 60 min in the oven - 20 does it for us when we
aren't having slide issues.

Also, if you are using any Sta-on or any adhesive in your waterbath with
the charged slides it can cause the slides to sort of repel the tissue
and negate the "charged" effect again certain tissues will
fall off, others stay on

Dana Spears, HTL(ASCP)
Laboratory Manager
Methodist Medical Center
(309) 672-4930 (office)
(309) 255-7214 (cell)
(309) 279-3768 (fax)
dspe...@mmci.org


Phyllis Thaxton  4/8/2009 8:43:36 AM >>>


Is anyone out there having problems with tissue washing off the slides
using the Ultraview kit from Ventana?

Mainly we are having problems with  needle biopsies washing off
(prostate, liver). Fixation and processing is always the same 4-6  hours
fixation, 3 hour biopsy processing run on VIP processor, cut no thicker
than 4 microns, airdried at least 30 minutes, baked at 59-60 for at
least one hour prior to staining. The instrument's calibrations have
been checked and are fine.

We never had this problem with the iView kit using the Nexes, doing
pretreatments offline.

Any help, ideas, info will be appreciated.

Thanks,
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 35401



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Re: [Histonet] onalighternote

[Joe Nocito]

how about having my name: Giuseppe, Pasquale, Enzo Guido Nocito. I think I 
was named after a pizza. That's why I call myself Joe. (The toes came later, 
heh,heh,heh)


JTT
- Original Message - 
From: "Bernie Taupin" 
To: "Va Paula Sicurello" ; "Jeanine 
(CDC/CCID/NCZVED)Bartlett" ; "R.E.Edwards" 
; 

Sent: Wednesday, April 01, 2009 8:13 AM
Subject: Re: [Histonet] onalighternote


I don't know what makes some of you people persist in making fun of a 
complete stranger's name, but I'll have you know I was born before the 
person you are talking about.






From: Va Paula Sicurello 
To: Jeanine (CDC/CCID/NCZVED)Bartlett ;  R.E.Edwards 
; histonet@lists.utsouthwestern.edu; Bernie Taupin 


Sent: Tuesday, March 31, 2009 7:01:14 PM
Subject: Re: [Histonet] onalighternote


Hey, we can't help it if your parents were big fans of his.  He is a great 
song writer.


;-)


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Tue, 3/31/09, Bernie Taupin  wrote:


From: Bernie Taupin 
Subject: Re: [Histonet] onalighternote
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." 
, histonet@lists.utsouthwestern.edu

Date: Tuesday, March 31, 2009, 4:52 PM
Yeah, hilarious.. I swear, I haven't
heart THAT one before





From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" 
To: "Edwards, R.E." ;
histonet@lists.utsouthwestern.edu
Sent: Tuesday, March 31, 2009 10:18:36 AM
Subject: RE: [Histonet] onalighternote

I wanted to ask how Elton was but thought that was a bit
too easy...



Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
On Behalf Of Edwards,
R.E.
Sent: Tuesday, March 31, 2009 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] onalighternote


I have  just  had  s from  Bernie
Taupin and Paul Schofield, is
this  a  record??.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
On Behalf Of Robyn
Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ford Royer

I think Ford gets the message...GET ON WITH IT!
Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
On Behalf Of Bernie
Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been
having a VERY bad day
indeed.

Although I can empathize that this might be the case, I do
find it
curious that we've heard nothing from Mr. Royer since his
little
outburst.

I, for one, wouldn't mind an apology to the list for your
antisocial
behaviour on it, Mr. Royer.




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Re: [Histonet] Off topic posts

[Joe Nocito]

yeah, what she said

- Original Message - 
From: "Bernice Frederick" 
To: "'Bernie Taupin'" ; "'Bryan Llewellyn'" 
; "'Histonet'" 

Sent: Wednesday, April 01, 2009 3:10 PM
Subject: RE: [Histonet] Off topic posts


How would we survive without those witticisms of JTT f we disallowed off
topic posts?
Joe?


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie
Taupin
Sent: Wednesday, April 01, 2009 1:46 PM
To: Bryan Llewellyn; Histonet
Subject: Re: [Histonet] Off topic posts

Who are you calling rude, Bruce? The people who made fun of my name?





From: Bryan Llewellyn 
To: Histonet 
Sent: Wednesday, April 1, 2009 2:26:02 PM
Subject: [Histonet] Off topic posts

Off topic posts have been allowed on Histonet since the very beginning.  I
am not aware that they have ever been banned.  After all, what are the TGIF
posts but completely off topic silliness for weekend stress relief.

Being rude to someone is something else, and shoukl be avoided whether on or

off topic.  That is just common courtesy.

Bryan Llewellyn


- Original Message - 
From: "Bernie Taupin" 

To: 
Sent: Wednesday, April 01, 2009 10:35 AM
Subject: Re: [Histonet] Let's call a truce



Nothing like yet another off-topic post to remind us not to post off-topic



posts.

Ahhh, sweet irony ;-)



Hello Netters,

Time to stop the current off topic conversations and > get back to
histology.

I apologize to Bernie for my comment since I
contributed to this off topic topic.

It's a new month let's start fresh.

Happy slicing and dicing and may all your stains work > perfectly.

Paula   :-)





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Re: [Histonet] Please help! In dire need of user manuals

[Joe Nocito]

OUCH

JTT
- Original Message - 
From: 

To: "Jennifer Anderson" 
Cc: "Histonet" 
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re: [Histonet] Please help! In dire need of user manuals


Dear Jennifer and any other Histonetters that are of like mind,

It's not quite "Flaming Friday" yet, but it will be in just a few hours,
so I'll go ahead a little early with what I hope is a reply that may
enlighten, be consultative, and hopefully not too offensive to those that
may be sensitive to such replies. Here it goes...

My First Question: You state that your lab has "purchased all used
equipment".  What vendor did you purchase from that did not provide
operator's manuals with the equipment?  This is unheard of amongst us
reputable used/refurbished equipment dealers so if I were you, I would not
recommend this vendor to others.

Now let's make sure that I have the facts straight... You have posted a
request that is seeking charity from members of this list that also
includes vendors.  In addition, not only are you asking for free copies of
publications that have been copyrighted and are the proprietary
information of the manufacturers that, at great R&D expenditure developed
them, but you are asking that the donor(s) mail hard copies (if available)
to you ...at the donor's expense for the shipping & handling.  Should
hard-copies not be available, you request the donor(s) to provide these
lengthy documents in digital PDF format... again with a substantial
expense to the donor(s) of converting these publications into digital
form.  Surprisingly, you have made this burdensome and expensive request
without any offer to reimburse the charitable donor(s) for their time and
expense to do all of this work for you.  In your final statement you then
wistfully hope that some benevolent vendor should raise their hand and
offer you a (free) copy and then insult that same vendor buy stating that
you "won't hold your breath for that".

My Second Question:  Are you crazy?


From your signature address; it appears that you are employed by a company

called Halozyme Therapeutics.  A quick web search informs us that Halozyme
is a for-profit California company that had revenues in the tens of
millions in 2008.  It was also exciting to learn that Halozyme is in Phase
2 clinical trials of their innovative insulin-PH20 with individuals that
suffer from Type 1 diabetes.  Very impressive.

Here is some good news for you.  I happen to have ALL the operator manuals
for the equipment that you are requesting.  I will copy them for you and
send them to you at my expense under one condition.  You state that if
someone can do this for you that you "will repay the favor, if at all
possible".  I have a family member that is a Type 1 insulin-dependent
diabetic.  All I ask in return is that you arrange with your marketing
department that they send me a one-year's supply of PH20 when it is
released to the public...  all at no charge, including shipping & handling
of course.

My Third Question:  Do we have a deal?

I look forward to your reply,

~ Ford

Ford "the demon vendor" Royer, MT(ASCP)
Histology Product Manager
Minnesota Medical, Inc. (a for-profit corporation just like yours)
Golden Valley, MN



Hello Everyone.



I our lab we've purchased all used equipment.  None of these instruments
came with a user's manual.  I am in need of a manual (hard-copy or PDF)
for the following:



Sakura Tissue-Tek VIP 3000

Leica Jung Histo Embedder

Microm HM335E Microtome



I do realize that requesting a copy of these is a lot to ask of someone.
It takes a lot of time to copy a 50-page manual.  I'll repay the favor,
if at all possible.  I'm hoping that a vendor may raise their hand and
offer a copy?  I won't hold my breath for that...



Thanks everyone.



Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11404 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

jander...@halozyme.com 






The information transmitted in this email is confidential and is intended
only for the person(s) or entity to which it is addressed.  Delivery of
this message to any person other than the intended recipient(s) is not
intended in any way to waive confidentiality or any applicable privilege.
Any review, retransmission, dissemination or other use of, or taking of
any action in reliance upon, this information by individuals or entities
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Re: [Histonet] Offended

[Joe Nocito]

here we go and it's almost Friday. I received a call just like that at my 
last job. Me being me, I gave her a piece of my mind ( not too much, I don't 
have that much to go around). Told her she had some big testicles (not the 
word I used, but you get the idea) and hung up. I then called in the tech 
and had a talk with him. Asked him if he was looking for a job. He told me 
that he wasn't and didn't know how she got his name. I told him that if he 
was looking for another job to do it on his time and on his dollar. If I 
ever received another call from a headhunter naming him, he would have to 
talk to them because he would be shown the door.
There are other ways to go about this. Calling a lab is not one of them, but 
I'm sure some people give their work number for different reasons, trying to 
use it as leverage for a pay raise, to get the supervisors nervous, because 
the techs are that brazen, or belong to the id10t  club. Let's face it, we 
are becoming a scarce breed and people have quotas to meet. Some will stop 
at nothing. That's my story and I'm sticking to it.


JTT
- Original Message - 
From: "Michael LaFriniere" 

To: 
Sent: Thursday, December 11, 2008 9:37 AM
Subject: [Histonet] Offended


I Wanted to obtained opinions of Managers/Supervisors in the histo world and 
how you handle situations regarding recruiters calling directly into 
Pathology Labs to specific tech's and stating they are seeking referrals for 
job placements. I recently had an AP staffing solution company call one of 
my labs to talk to any "histologist"  I felt was to recruit staff.  I feel 
this is an unprofessional practice to call directly into the laboratory and 
to hide behind the wording "referrals" when actually it appears they are 
trying to get information from the tech on the phone seeing if they are 
looking for a "new job".  I feel that this is an intrusion as well as 
inappropriate in our line of business and we are not in the business to give 
referrals to companies in the staffing solution arena for their monetary 
gain as well as trying to entice  our staff. What the staff does with these 
types of companies on personal time is their business, however, I think it 
is offending for companies to demonstrate this behavior.  If anyone would 
like to know which company and person called into my lab I will personally 
inform you on private email!


Michael LaFriniere
Executive Director
CSM
Washington DC/Maryland/Virginia

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Re: [Histonet] JCAHO

[Joe Nocito]

can you say "GRINCH"?

- Original Message - 
From: "Ingles Claire " <[EMAIL PROTECTED]>

To: "Merced Leiker" <[EMAIL PROTECTED]>
Cc: 
Sent: Thursday, December 04, 2008 2:08 PM
Subject: RE: [Histonet] JCAHO


Yesterday was the closest I have ever come to going 'Histo'. After 2 pretty 
much full days of being the only one to decorate the clinic area. Making 
many of the garlands, not just throwing something on the wall. Buying some 
of the
stuff myself, and being off the clock for some of it (I am proud of all my 
work, not just slide production). The powers that be have stated that 
because of fire hazard concerns relayed by the JCAHO inspectors there are 
not supposed to be any decorations on walls, ceilings, or door frames. It 
took me an hour just to take everything down. I was so miffed (an 
understatement) I went home earlier than usual. I also boycotted the 
'holiday party' today. ooh, missed out on the cousins subs and sheet cake.
Sorry. Still trying to get ahold of the ?logic? involved with this. Not to 
mention all my wasted time and energy.  Thank heavens today is my Friday. I 
need to get back in the holiday spirit somehow.


Claire
HTL moonlighting as an unappreciated Interior Decorator



Sounds like you found out the hard way?


--On Wednesday, December 03, 2008 6:07 PM -0600 Ingles Claire
<[EMAIL PROTECTED]> wrote:



FYI Everyone:
If you are still waiting for your inspection, don't bother putting up
Christmas decorations.  Claire





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Re: [Histonet] Blue haze

[Joe Nocito]

lunch!?
- Original Message - 
From: "Edwards, R.E." <[EMAIL PROTECTED]>
To: "'Bernice Frederick'" <[EMAIL PROTECTED]>; "'Joe Nocito'" 
<[EMAIL PROTECTED]>; "'Histonet Alias'" <[EMAIL PROTECTED]>; 
"'Marshall, Kimberly'" <[EMAIL PROTECTED]>

Cc: 
Sent: Thursday, December 04, 2008 10:52 AM
Subject: RE: [Histonet] Blue haze


Hey  Joe, where  you  going  with  that  toe in  your hand??

-Original Message-
From: [EMAIL PROTECTED] 
[mailto:[EMAIL PROTECTED] On Behalf Of Bernice 
Frederick

Sent: 04 December 2008 14:06
To: 'Joe Nocito'; 'Histonet Alias'; 'Marshall, Kimberly'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blue haze

I'm sure you'll just charm them and they'll never catch on


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Joe Nocito
Sent: Wednesday, December 03, 2008 8:31 PM
To: Histonet Alias; Marshall, Kimberly
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Blue haze

I thought that was Purple Haze, by Jimi Hendrix. Oh dear me, is it Friday
yet? Forgive me, we are in the window for CAP and I'm just all in a haze.

JTT
- Original Message - 
From: "Histonet Alias" <[EMAIL PROTECTED]>

To: "Marshall, Kimberly" <[EMAIL PROTECTED]>
Cc: 
Sent: Wednesday, December 03, 2008 12:08 PM
Subject: Re: [Histonet] Blue haze



Hi Kimberly,
Are you using a clarifier step?

On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly <
[EMAIL PROTECTED]> wrote:


Howdy all,

  I have just changed over to Surgi Path's H & E system.  With the latest
fear of running out of hematoxylin and all my Pathologist wanted us to
change over.  Its a great stain but we have a bad "haze"  in the
background.
 It is not hurting the tissue or DX at all, but to ME it looks awful.  Is
there anyone else out there using this?   We are not using positive
charged
slides or anything added to my water bath.  Any suggestions  Thanks
in
advance

Kimberly Marshall HT (ASCP)





==

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confidential
and protected from disclosure.  If the reader of this message is not the
intended
recipient or an employee or agent responsible for delivering this message
to the
intended recipient, you are hereby notified that any dissemination,
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or copying of this communication is strictly prohibited.  If you have
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--
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States
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Re: [Histonet] Blue haze

[Joe Nocito]

I thought that was Purple Haze, by Jimi Hendrix. Oh dear me, is it Friday 
yet? Forgive me, we are in the window for CAP and I'm just all in a haze.


JTT
- Original Message - 
From: "Histonet Alias" <[EMAIL PROTECTED]>

To: "Marshall, Kimberly" <[EMAIL PROTECTED]>
Cc: 
Sent: Wednesday, December 03, 2008 12:08 PM
Subject: Re: [Histonet] Blue haze



Hi Kimberly,
Are you using a clarifier step?

On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly <
[EMAIL PROTECTED]> wrote:


Howdy all,

  I have just changed over to Surgi Path's H & E system.  With the latest
fear of running out of hematoxylin and all my Pathologist wanted us to
change over.  Its a great stain but we have a bad "haze"  in the 
background.

 It is not hurting the tissue or DX at all, but to ME it looks awful.  Is
there anyone else out there using this?   We are not using positive 
charged
slides or anything added to my water bath.  Any suggestions  Thanks 
in

advance

Kimberly Marshall HT (ASCP)


==
The information contained in this message may be privileged and/or
confidential
and protected from disclosure.  If the reader of this message is not the
intended
recipient or an employee or agent responsible for delivering this message
to the
intended recipient, you are hereby notified that any dissemination,
distribution
or copying of this communication is strictly prohibited.  If you have
received this
communication in error, please notify the sender immediately by replying 
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this
message and deleting the material from any computer.


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--
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States
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Re: [Histonet] Large Slide Boxes

[Joe Nocito]

have you tried Lab Storages Systems, New Comer's Supply or Surgipath?

JTT
- Original Message - 
From: "Pamela Marcum" <[EMAIL PROTECTED]>

To: 
Sent: Tuesday, December 02, 2008 3:40 PM
Subject: [Histonet] Large Slide Boxes




Good Afternoon All,



I am looking to purchase some 3 inch by 2 inch (3X2) slide boxes for large 
slides. We do some larger specimens and it seems these have become almost 
impossible to find. I have tried several sources and no one had them or knew 
where I could get them at this time. I have tried myneurolab and several 
other speciality vendors also to no avail. HELP




Pam Marcum
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Re: [Histonet] Ventana Benchmark XTs

[Joe Nocito]

I have to agree with Al ( can I call you Al, Alias?) on this one. Remember 
when I had to sign off of the Histonet a couple of years ago because a 
certain company contacted my CEO and filed a complaint. I almost lost my job 
because I posted an honest opinion. An opinion that was greatly shared if I 
remember correctly. In this era of financial uncertainties, foreclosures and 
all, I don't blame Al. I'm sure he/she has bills just the rest of us do. 
Bills that won't go away if he/she loses the job.

That's my story and I'm sticking to it.

Joe Nocito BS, PA, HT(ASCP)QIHC
San Antonio, TX

- Original Message - 
From: "Histonet Alias" <[EMAIL PROTECTED]>

To: "Della Speranza, Vinnie" <[EMAIL PROTECTED]>
Cc: ; "Tracey Lenek" 
<[EMAIL PROTECTED]>; "Burton,Lynn" <[EMAIL PROTECTED]>; "Joanna 
Bartczak-McKay" <[EMAIL PROTECTED]>; "Martin Trotter" 
<[EMAIL PROTECTED]>

Sent: Tuesday, December 02, 2008 1:19 PM
Subject: Re: [Histonet] Ventana Benchmark XTs


Mr. Della Speranza, I respect what you have done for the histology 
community
and we have had pleasant conversations in the past but you can not speak 
for
the Histonet members. You act as if I am hiding my identity to create 
havoc

and disruption. I have not done this nor will I do this. I have been on
histonet for many years and I will continue to do so. As I explained in my
previous posts my current employer does not want any opinions, help, or
questions associated with the laboratory. That is the only reason that I
have chosen to remain anonymous. Would it make anyone feel better if I 
made

up a fake name and lab as my signature?  I will continue to post and speak
freely without breaking any rules of this listserver. If I respond or ask 
a

question, those who find my years of experience not "credible" then
I apologize.

On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie 
<[EMAIL PROTECTED]>wrote:



I'm afraid your opinion will not be credible as long as you insist on
remaining anonymous. It has nothing to do with whether you like or 
dislike a

particular product or instrument.  Histonet members appreciate the
responsibility that comes with posting one's views or knowledge by
identifying themselves accordingly. In my opinion anyone who insists on
remaining anonymous should not be permitted to participate on the list.


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974


-Original Message-
From: [EMAIL PROTECTED] [mailto:
[EMAIL PROTECTED] On Behalf Of Histonet Alias
Sent: Saturday, November 29, 2008 11:03 AM
To: Mark Tarango
Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
Burton, Lynn; Joanna Bartczak-McKay
Subject: Re: [Histonet] Ventana Benchmark XTs

Sorry I don't work for Ventana and I have no beef with them. It
may disappoint some that I have not had a bad experience. So my opinion
does
not count because I have had a different experience with my XT than you? 
I
use the name of The Unknown HT so I can express my opinions freely 
without

my employer receiving phone calls because certain people don't like my
opinions.

On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango <[EMAIL PROTECTED]>
wrote:

> I don't know how much the opinion of "The Unknown HT(ASCP)" counts. 
> You

> aren't with Ventana I hope.
>
> Mark
>
> On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
[EMAIL PROTECTED]>wrote:
>
>> They are switching to a more reliable manufacturer. They are being
>> proactive
>> and switching them before they fail down the road. My XT has been a
>> workhorse but we know certain peoples opinions about the company on
here.
>> I
>> will take it any day over the rest.
>>
>> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn 
>> <[EMAIL PROTECTED]

>> >wrote:
>>
>> > We have had some trouble but they are planning to replace them. We
>> received
>> > a letter that they had changed vendors for parts and found they need
to
>> > switch back. They did replace one section earlier when it stopped
>> working
>> > completely.
>> >
>> > Lynn Burton
>> > Lab Assoc. I
>> > Animal Disease Lab
>> > Galesburg, Il 61401
>> >
>> > 
>> >
>> > From: [EMAIL PROTECTED] on behalf of Tracey
>> Lenek
>> > Sent: Wed 11/26/2008 10:59 AM
>> > To: histonet@lists.utsouthwestern.edu
>> > Cc: Martin Trotter; Joanna Bartczak-McKay
>> > Subject: [Histonet] Ventana Benchmark XTs
>> >
>> >
>> >
>>

Re: [Histonet] Histo humor :-)

[Joe Nocito]

is a casual histo tech like a  casual end table? What is it the rest of the 
time?


Oh, yeah, we not only have a great cents of humor, we're real cut ups to.

JTT
- Original Message - 
From: "Dianne Holmes" <[EMAIL PROTECTED]>

To: "Histonet" 
Sent: Tuesday, December 02, 2008 10:44 AM
Subject: [Histonet] Histo humor :-)


Not only do I get valuable info from this group but histologists have a 
GREAT sense of humor in common !!

I loved the 'casual histo tech' volley!!


Individuals who have received this information in error or are not 
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Re: Re: [Histonet] Ventana Benchmark XTs

[Joe Nocito]

nope, the hound of Tucson
  - Original Message - 
  From: Steven Hacker 
  To: [EMAIL PROTECTED] 
  Cc: [EMAIL PROTECTED] ; histonet@lists.utsouthwestern.edu ; [EMAIL PROTECTED] 
; [EMAIL PROTECTED] ; [EMAIL PROTECTED] ; [EMAIL PROTECTED] 
  Sent: Friday, November 28, 2008 10:13 PM
  Subject: Re: Re: [Histonet] Ventana Benchmark XTs


  The Hound of the Baskerville?


  Nov 28, 2008 08:51:46 PM, [EMAIL PROTECTED] wrote:

Yup, been through that before. I made sure that we switched to BioCare's
reagents on a dako-like stainer after that. I would think that'd be the
only reason to hide your identity (fear of Ventana sending the hounds after
you).

On Fri, Nov 28, 2008 at 3:19 PM, Joe Nocito wrote:

> no, they usually contact the CEO's of the labs to get their employees to
> stop ragging on the company. Then again, you never know who is lurking in
> the winds.
>
> JTT
> - Original Message - From: "Mark Tarango" 
> To: "Histonet Alias" 
> Cc: ; "Martin Trotter" 
> [EMAIL PROTECTED]>; "Tracey Lenek" ;
> "Burton,Lynn" ; "Joanna Bartczak-McKay" 
> [EMAIL PROTECTED]>
> Sent: Friday, November 28, 2008 2:10 PM
> Subject: Re: [Histonet] Ventana Benchmark XTs
>
>
> I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You
>> aren't with Ventana I hope.
>>
>> Mark
>>
>> On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias 
>> >wrote:
>>
>> They are switching to a more reliable manufacturer. They are being
>>> proactive
>>> and switching them before they fail down the road. My XT has been a
>>> workhorse but we know certain peoples opinions about the company on 
here.
>>> I
>>> will take it any day over the rest.
>>>
>>> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn 
>>> >wrote:
>>>
>>> > We have had some trouble but they are planning to replace them. We
>>> received
>>> > a letter that they had changed vendors for parts and found they need 
to
>>> > switch back. They did replace one section earlier when it stopped >
>>> working
>>> > completely.
>>> >
>>> > Lynn Burton
>>> > Lab Assoc. I
>>> > Animal Disease Lab
>>> > Galesburg, Il 61401
>>> >
>>> > 
>>> >
>>> > From: [EMAIL PROTECTED] on behalf of Tracey
>>> Lenek
>>> > Sent: Wed 11/26/2008 10:59 AM
>>> > To: histonet@lists.utsouthwestern.edu
>>> > Cc: Martin Trotter; Joanna Bartczak-McKay
>>> > Subject: [Histonet] Ventana Benchmark XTs
>>> >
>>> >
>>> >
>>> > Hi,
>>> >
>>> > We have had on-going thermal slide pad issues with our XTs since they
>>> were
>>> > installed
>>> > in February. The slide tray assemblies have been replaced not once but
>>> > twice and we are still having inconsistent
>>> > results with the temp verifier slides. Has anyone experienced the same
>>> > issues with this
>>> > instrumentation?
>>> >
>>> > Thanks
>>> > Tracey Lenek
>>> > Tech III - Anatomic Pathology
>>> > Calgary Laboratory Services
>>> > 403-770-3588
>>> >
>>> > 
>>> > This message and any attached documents are only for the use of the
>>> > intended recipient(s), are confidential and may contain privileged
>>> > information. Any unauthorized review, use, retransmission, or other
>>> > disclosure is strictly prohibited. If you have received this message 
in
>>> > error, please notify the sender immediately, and then delete the >
>>> original
>>> > message. Thank you.
>>> > ___
>>> > Histonet mailing list
>>> > Histonet@lists.utsouthwestern.edu
>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>> >
>>> >
>>> > ___
>>> > Histonet mailing list
>>> > Histonet@lists.utsouthwestern.edu
>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>> >
>>>
>>>
>>>
>>> --
>>> The Unknown HT(ASCP)
>>> ___
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>>>
>>> ___
>> Histonet mailing list
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>>
>
>
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Re: [Histonet] Ventana Benchmark XTs

[Joe Nocito]

no, they usually contact the CEO's of the labs to get their employees to 
stop ragging on the company. Then again, you never know who is lurking in 
the winds.


JTT
- Original Message - 
From: "Mark Tarango" <[EMAIL PROTECTED]>

To: "Histonet Alias" <[EMAIL PROTECTED]>
Cc: ; "Martin Trotter" 
<[EMAIL PROTECTED]>; "Tracey Lenek" <[EMAIL PROTECTED]>; 
"Burton,Lynn" <[EMAIL PROTECTED]>; "Joanna Bartczak-McKay" 
<[EMAIL PROTECTED]>

Sent: Friday, November 28, 2008 2:10 PM
Subject: Re: [Histonet] Ventana Benchmark XTs



I don't know how much the opinion of "The Unknown HT(ASCP)" counts.  You
aren't with Ventana I hope.

Mark

On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias 
<[EMAIL PROTECTED]>wrote:



They are switching to a more reliable manufacturer. They are being
proactive
and switching them before they fail down the road. My XT has been a
workhorse but we know certain peoples opinions about the company on here. 
I

will take it any day over the rest.

On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn <[EMAIL PROTECTED]
>wrote:

> We have had some trouble but they are planning to replace them. We
received
> a letter that they had changed vendors for parts and found they need to
> switch back. They did replace one section earlier when it stopped 
> working

> completely.
>
> Lynn Burton
> Lab Assoc. I
> Animal Disease Lab
> Galesburg, Il 61401
>
> 
>
> From: [EMAIL PROTECTED] on behalf of Tracey
Lenek
> Sent: Wed 11/26/2008 10:59 AM
> To: histonet@lists.utsouthwestern.edu
> Cc: Martin Trotter; Joanna Bartczak-McKay
> Subject: [Histonet] Ventana Benchmark XTs
>
>
>
> Hi,
>
> We have had on-going thermal slide pad issues with our XTs since they
were
> installed
> in February. The slide tray assemblies have been replaced not once but
> twice and we are still having inconsistent
> results with the temp verifier slides.  Has anyone experienced the same
> issues with this
> instrumentation?
>
> Thanks
> Tracey Lenek
> Tech III - Anatomic Pathology
> Calgary Laboratory Services
> 403-770-3588
>
> 
> This message and any attached documents are only for the use of the
> intended recipient(s), are confidential and may contain privileged
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> original

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--
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Re: [Histonet] Ventana Benchmark XTs

[Joe Nocito]

oh yeah, many times. I had three at my last place. One was always going down 
for one reason or another. I wanted them to replace the machine, but they 
kept putting bandages on a bleeding wound. I went up to the regional 
maintenance manager and never got an answer.
I guess the Lemon Law doesn't apply to Ventana. Of course, a lot of things 
don't apply to Ventana now, does it?


JTT
- Original Message - 
From: "Tracey Lenek" <[EMAIL PROTECTED]>

To: 
Cc: "Martin Trotter" <[EMAIL PROTECTED]>; "Joanna Bartczak-McKay" 
<[EMAIL PROTECTED]>

Sent: Wednesday, November 26, 2008 10:59 AM
Subject: [Histonet] Ventana Benchmark XTs


Hi,

We have had on-going thermal slide pad issues with our XTs since they were 
installed
in February. The slide tray assemblies have been replaced not once but twice 
and we are still having inconsistent
results with the temp verifier slides.  Has anyone experienced the same 
issues with this

instrumentation?

Thanks
Tracey Lenek
Tech III - Anatomic Pathology
Calgary Laboratory Services
403-770-3588


This message and any attached documents are only for the use of the intended 
recipient(s), are confidential and may contain privileged information. Any 
unauthorized review, use, retransmission, or other disclosure is strictly 
prohibited. If you have received this message in error, please notify the 
sender immediately, and then delete the original message. Thank you.

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Re: [Histonet] Processing problems

[Joe Nocito]

it might be your processor.  Depending on the type of processor, some 
processors have a rotary valve that rotates from reagent bottle to reagent 
bottle. When the rotary valves fails, it starts placing a reagent in the 
reagents bottles indiscriminately. For instance, our processor started 
spitting paraffin in our alcohols.


JTT
- Original Message - 
From: "Genest, Sharon SktnHR" <[EMAIL PROTECTED]>

To: 
Sent: Wednesday, November 19, 2008 3:15 PM
Subject: [Histonet] Processing problems


We had a problem with our recycler and xylene contamination so we now
test each batch  by adding water to a sample of recycled alcohol. If it
turns cloudy we assumed that we had xylene contamination.
We have lately tested commercial ethanols (no turbidity)before placing
them on the processor and after we processing with them just once we
have turbidity after adding a water to all the ethanols 70-100%.
This is happening to all the alcohols on all of our processors. I am
thinking that it is not a xylene contamination but something else maybe
a leaching of plastic from the cassettes? Has this happened to anyone
else?  Can anyone give me any suggestions.
Sharon
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Re: [Histonet] JCAHO question

[Joe Nocito]

Jessica,
in my experience, CAP trumps JCAHO. Every hospital that I've worked in that 
the lab was accredited by CAP, JCAHO left the lab alone, except for taking a 
walk through and maybe asking some safety questions.


JTT
- Original Message - 
From: "Vacca Jessica" <[EMAIL PROTECTED]>

To: 
Sent: Monday, November 17, 2008 1:05 PM
Subject: [Histonet] JCAHO question


I was presented by my lab manager this question when it comes to F.S. and 
FNA's, - The key word here is THE ORDERING OF: Does anyone monitor the time 
in which the specimen is  removed from the OR to the time it is called back. 
Our specimens are delivered to us and clocked in at the time of receipt by 
the lab to the time in which it is resulted back. In CAP it does not state 
the "start" in which the TAT is monitored. We are both CAP and JCAHO, Do you 
have 2 separate policies or do you just use your CAP policy?


NPSG.02.03.01
The [organization] measures, assesses, and, if needed, takes action to 
improve the timeliness of reporting, and the timeliness of receipt of 
critical tests and critical results and values by the responsible licensed 
caregiver.

Elements of Performance for NPSG.02.03.01
1 The laboratory defines critical tests and critical results and values.
2 The laboratory defines the acceptable length of time between the ordering 
of critical tests and reporting the results of these tests, whether normal 
or abnormal.
3 The laboratory defines the acceptable length of time for reporting the 
results of routine tests with critical abnormal values or findings.
4 The laboratory defines the acceptable length of time between the 
availability of critical tests and critical results and values and receipt 
by the responsible licensed caregiver.
5 The laboratory collects data on the timeliness of reporting critical test 
results and critical results and values from routine tests.
6 The laboratory assesses the data on the timeliness of reporting critical 
test results and critical results and values from routine tests and 
determines whether a need for improvement exists.
7 The laboratory takes appropriate action to improve the timeliness of 
reporting critical test results and critical results and values from routine 
tests and measures the effectiveness of those actions.
8 Critically abnormal test results are communicated quickly to a responsible 
licensed caregiver so that prompt action may be taken.
9 When the responsible licensed caregiver is not available, a back-up 
reporting system provides the information in a timely manner to another 
qualified responsible caregiver to prevent avoidable delays in treatment or 
response.


Jessica Vacca
Histology Supervisor
119 Oakfield Dr
Brandon Fl 33511
(813) 571-5193
(813) 571-5169 FAX



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Re: [Histonet] RA Lamb has been sold to the evil empire

[Joe Nocito]

great. Soon there will be only one booth at the states and the NSH 
conventions



- Original Message - 
From: "Bernice Frederick" <[EMAIL PROTECTED]>
To: "'Jack Cates'" <[EMAIL PROTECTED]>; 


Sent: Wednesday, November 12, 2008 2:46 PM
Subject: RE: [Histonet] RA Lamb has been sold to the evil empire


I keep telling everyone it's going to be "histosupplies are us" one of these
days!!!
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Jack Cates
Sent: Wednesday, November 12, 2008 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RA Lamb has been sold to the evil empire

I am very sorry to see this happen again to a great company who bent over
backwards to assist customers. I hope ThemoShandonFisherRichardRaymond (to
be continued) will start to keep the parts that made Richard Allen, Raymond
A. Lamb and others great to work with

I am also very sorry to see Tonia and Jerry go.

Tuesday, Nov. 11, 2008
Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with
Acquisition of Raymond A. Lamb
WALTHAM, Mass. - Thermo Fisher Scientific Inc., the world leader in serving
science, today announced it has acquired Raymond A. Lamb Ltd., a
manufacturer of
histology and anatomical pathology products based in Eastbourne, U.K., near
London.
In business since 1964, Raymond A. Lamb has grown to become a global
provider of
products for the pathology laboratory. Recently, its focus has been on
systems
that automate the labeling and tracking of slides and cassettes that carry
patient tissue samples, reducing the possibility of errors during their
processing, storage and retrieval. The company has had a longstanding
relationship with the anatomical pathology business of Thermo Fisher as a
supplier of labeling products for cassettes and glass slides, as well as
other
anatomical pathology equipment.
"Our anatomical pathology customers - in both clinical and research markets
-
are demanding better solutions for tracking the increasing number of samples
that they need to process and diagnose," said Marijn E. Dekkers, president
and
chief executive officer of Thermo Fisher Scientific. "This acquisition
brings
new-generation systems that can be integrated with our existing portfolio of
anatomical pathology equipment and consumables to create a more efficient,
and
reliable, workflow."
Raymond A. Lamb had revenues of approximately $9 million in 2007, and will
be
integrated into Thermo Fisher's Analytical Technologies Segment.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving
science, enabling our customers to make the world healthier, cleaner and
safer.
With annual revenues of $10 billion, we have more than 30,000 employees and
serve over 350,000 customers within pharmaceutical and biotech companies,
hospitals and clinical diagnostic labs, universities, research institutions
and
government agencies, as well as environmental and industrial process control
settings. Serving customers through two premier brands, Thermo Scientific
and
Fisher Scientific, we help solve analytical challenges from routine testing
to
complex research and discovery. Thermo Scientific offers customers a
complete
range of high-end analytical instruments as well as laboratory equipment,
software, services, consumables and reagents to enable integrated laboratory
workflow solutions. Fisher Scientific provides a complete portfolio of
laboratory equipment, chemicals, supplies and
services used in healthcare, scientific research, safety and education.
Together, we offer the most convenient purchasing options to customers and
continuously advance our technologies to accelerate the pace of scientific
discovery, enhance value for customers and fuel growth for shareholders and
employees alike. Visit www.thermofisher.com.
Advertisement
The following constitutes a "Safe Harbor" statement under the Private
Securities
Litigation Reform Act of 1995: This press release contains forward-looking
statements that involve a number of risks and uncertainties. Important
factors
that could cause actual results to differ materially from those indicated by
such forward-looking statements are set forth in the company's Quarterly
Report
on Form 10-Q for the quarter ended September 27, 2008, under the caption
"Risk
Factors," which is on file with the Securities and Exchange Commission (SEC)
and
available in the "Investors" section of our Website under the heading "SEC
Filings." Important factors that could cause actual results to differ
materially
from those indicated by forward-looking statements include risks and
uncertainties relating to: competition and its effect on pricing, spending,
third-party relationships and revenues; the need to develop new products and
adapt to significant technological
ch

Re: [Histonet] REMOVE FROM LIST

[Joe Nocito]

blood pressure medicine, geez, I want some of that Gold Seal alcohol. And I 
don't want to unsubscribe (did I spell that right?)


JTT
- Original Message - 
From: "Victor Tobias" <[EMAIL PROTECTED]>

To: "Ford Royer" <[EMAIL PROTECTED]>
Cc: 
Sent: Thursday, November 06, 2008 4:25 PM
Subject: Re: [Histonet] REMOVE FROM LIST



Ford,

I hope you took your blood pressure medication today?

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
[EMAIL PROTECTED]
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use of 
the intended recipients. If you are not the intended recipient, or if the 
message has been addressed to you in error, do not read, disclose, 
reproduce, distribute, disseminate or otherwise use this transmission. 
Instead, please notify the sender by reply e-mail, and then destroy all 
copies of the message and any attachments.




Ford Royer wrote:

You have GOT to be kidding! ... someone's just has to be pulling our leg.
;-)


(If not. Ellen, click on the link that is shown at the very bottom of 
this
page, and read what it says when you get to the web page that it is 
linked

to. I wish you the very best, and good luck to you.)

Ford M. Royer, MT(ASCP)

Minnesota Medical, Inc.


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Ellen
Pearlstein
Sent: Thursday, November 06, 2008 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] REMOVE FROM LIST



please remove me from the list.


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Re: [Histonet] 200 proof alcohol?

[Joe Nocito]

I'm allergic to blue cheese.

- Original Message - 
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <[EMAIL PROTECTED]>
To: "Joe Nocito" <[EMAIL PROTECTED]>; "Caroline Bass" <[EMAIL PROTECTED]>; 


Sent: Thursday, November 06, 2008 4:41 AM
Subject: RE: [Histonet] 200 proof alcohol?


I like mine with blue-cheese olives but to each his/her own.


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED] 
[mailto:[EMAIL PROTECTED] On Behalf Of Joe Nocito

Sent: Wednesday, November 05, 2008 7:23 PM
To: Caroline Bass; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] 200 proof alcohol?

200 proof is 200 proof, can't get any purer than that. Of course, I would 
take the taste test first. Green olives stuffed with Feta cheese goes good.


JTT
- Original Message -
From: "Caroline Bass" <[EMAIL PROTECTED]>
To: 
Sent: Wednesday, November 05, 2008 11:15 AM
Subject: [Histonet] 200 proof alcohol?


Hello,

I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically the same
as molecular biology grade? How about histology grade? I seem to recall
using hospital provided ethanol for molecular biology experiments in my
postdoc, so I think it should be ok.

Any advice is appreciated!

Caroline
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Re: [Histonet] 200 proof alcohol?

[Joe Nocito]

200 proof is 200 proof, can't get any purer than that. Of course, I would 
take the taste test first. Green olives stuffed with Feta cheese goes good.


JTT
- Original Message - 
From: "Caroline Bass" <[EMAIL PROTECTED]>

To: 
Sent: Wednesday, November 05, 2008 11:15 AM
Subject: [Histonet] 200 proof alcohol?


Hello,

I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically the same
as molecular biology grade? How about histology grade? I seem to recall
using hospital provided ethanol for molecular biology experiments in my
postdoc, so I think it should be ok.

Any advice is appreciated!

Caroline
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Re: [Histonet] Nails

[Joe Nocito]

it's a good thing you asked.
I let my nails (well not my nails personally, but the specimen nails) soak 
in 20% ammonium hydroxide or 20% sodium hydroxide for at least 1 hour 
(sodium hydroxide works better, but whatever you can get). Then I rinse the 
blocks in running water for 2-3 minutes, then place in formalin for normal 
processing. When cutting, I use positive slides with a waterbath filled with 
distilled water (if you use an adhesive, you will be defeating the purpose 
of the charged slides) cut at 4 microns and place in an 80 C oven for 15 
minutes. Make sure that the slides are completely dried before beginning 
staining (if the slides are still  a little wet, I put them in front of a 
small fan to blow off all the water). My lab performs at least 10 blocks per 
day and we don't have problems with the H&E or the PAS/fungus. Hey, that's 
why they call me Joe, the Toe.


JTT
- Original Message - 
From: "Godfrey Guerzon" <[EMAIL PROTECTED]>

To: 
Sent: Tuesday, October 14, 2008 4:54 PM
Subject: [Histonet] Nails


We are having problems getting nail sections to stick to the slide. They 
almost always fall off the slide during H&E staining. We use plus slides and 
various adhesives - sta-on, hairspray, etc. with no success.


Is there anybody that can help me by sharing their secret method to get nail 
sections survibe H&E staining without falling off?


Please help

Thanks.

Godfrey




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