Re: [Histonet] tissue cassettes

[Kurth, Virginia L via Histonet]

I am old school and prefer them dry, lol.  I agree with Thomas, that shouldn't 
have that affect.

Ginny


-Original Message-
From: Thomas Podawiltz via Histonet 
Sent: Friday, February 9, 2024 8:34 AM
To: Brazie, Jeneanne E *HS ; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] tissue cassettes

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Without seeing the blocks, that sounds more like cold molds being used, more 
Then, whether or not the tissues are kept in a dry, hot, well, or a wet well.


Sent from Yahoo Mail for iPad


On Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet 
 wrote:

Hello :) I am encountering push back in our lab when I fill the embedding units 
with melted paraffin in the embedding wells. The techs here like for the tissue 
cassettes  to sit dry (no wax) while in the  embedding units. I find that the 
tissue rolls out of the sections while cutting because of a layering effect 
between the tissue and the paraffin its embedded in. I have communicated this 
but they tell me I'm "old school". Does anyone have any thoughts or opinions on 
this topic??

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Re: [Histonet] Retic help

[Kurth, Virginia L via Histonet]

Same here, we moved to a new location and the PH of the water was too acidic, 
the DI water would remove the silver and leave it yellowed.   Some solutions 
that I was making that usually would take hours to go into solution on the stir 
plate went immediately.

Good Luck!

Ginny Kurth, HT, (ASCP)CM


-Original Message-
From: Bacon, Charles via Histonet 
Sent: Saturday, September 16, 2023 2:15 PM
To: Steven Crochiere 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retic help

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Hi Steven,

We ran into this issue and it was the pH of the water. We were using our 
sterile line from the facility but the pH was too high. We used tap and it 
worked fine. This will also effect your stock solutions if you make them 
yourself so be aware of that as well.

Sent from my iPhone

> On Sep 15, 2023, at 4:02 PM, Steven Crochiere  wrote:
>
> Any advice on getting my retic stain to work properly is appreciated.
> Currently coming out a washed out gray overall, with proper staining
> at the periphery of the section. Thanks
>
> Sent via the Samsung Galaxy, an AT&T 4G LTE smartphone Get Outlook for
> Android F%2Furldefense.com%2Fv3%2F__https%3A%2F%2Faka.ms%2FAAb9ysg__%3B!!P_vj-
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Re: [Histonet] Sections sticking to tweezers

[Kurth, Virginia L via Histonet]

You may try to turn your water bath down a few degrees and see if this helps 
and what is the melting point of the paraffin you are using?  Most likely a 
paraffin/heat situation.
 If I  run into this issue, a quick fix is I rinse my forceps into the  ice 
water, wipe, and the sections will not stick.

Good Luck.

Ginny Kurth
UWHEALTH

-Original Message-
From: Ken M via Histonet 
Sent: Saturday, May 6, 2023 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sections sticking to tweezers

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Can anyone tell me why my sections are sticking to my tweezers and turning into 
one long string of snot when I try to separate them? I have a good pair of 
curved stainless tweezers and I am trying both the front and the back and 
tapping sharply while allowing the tweezers to open at the separation point. I 
can separate one or two, then it sticks and pulls up 7 or 8 sections into one 
long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. 
Even when I go to 6 it still does it. Any ideas?

Ken
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Re: [Histonet] Mice/Rat brain

[Kurth, Virginia L via Histonet]

I remember adding a couple drops of ETOH to the water bath; it's been a while 
so I am not sure the exact protocol.  (it will spin the tissue).  

Virginia Kurth, HT, (ASCP)CM
UW Health Surgical Pathology
Madison, WI  53792




 

-Original Message-
From: Renee Fisher via Histonet  
Sent: Thursday, March 9, 2023 8:51 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mice/Rat brain

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We are having issues with mice and rat brain sections. There are folds in the 
tissue. We just can't seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
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[Histonet] anti-HGAL

[Kurth, Virginia L via Histonet]

Does anyone use HGAL on an Ultra and would be willing to share information?

Thanks!


Ginny Kurth, HT, (ASCP)CM
Developmental Specialist
UW Health Surgical Pathology
 Highland Ave, WIMR MB1707.6
Madison, WI  53792
vku...@uwhealth.org






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Re: [Histonet] IHC staining of cartilage

[Kurth, Virginia L via Histonet]

Make sure they sit at room temperature longer than normal and then dry them 
longer.  I found that cutting them thinner (3 microns) also helps. 



Ginny Kurth, HT, (ASCP)CM
UW Health Surgical Pathology
 Highland Ave
Madison, WI 






-Original Message-
From: Hobbs, Carl via Histonet  
Sent: Tuesday, March 01, 2022 1:30 PM
To: histonet 
Subject: Re: [Histonet] IHC staining of cartilage

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system.
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Yes...a big problem for me, tooif you are using HIER??
Cartilage and bone are major tissue retention problems I cut Pwax sections of 
mouse long bones/knee joints for anti GFP IHC I still get a % fall off but,  
using manual HIER at 90C I manage to get enough sections to get good results 
However, I imagine that your autoIHC stainer prob uses 90C HIER?
I find Trajan 3 series slides more reliable than Superfrost Plus slides.
Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we can't 
turn ours off/down) Then I place slides in a 60C oven for 2hrs.
Then dewax etc

Be interesting to read of other suggestions

Best wishes

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL



020 7848 6810
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[Histonet] ADH5

[Kurth Virginia L. via Histonet]

Does anyone know if there is a reference lab that performs ADH5.  (particular 
the BioCare multiplex stain?)

Thanks Ginny



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[Histonet] Prostate whole mounts

[Kurth Virginia L. via Histonet]

  1.  As standard of care, do you process prostates as whole mounts (i.e. mega 
cassettes)?
  2.  Are there a subset of prostate resection cases that are processed in this 
manner (i.e. based on dx)?
  3.  Do you have any specimen types that are processed as whole mounts as 
standard of care?
  4.  How are these slides/blocks stored in conjunction with routine 
slide/block storage?
  5.  Are you aware of how radiologic/pathologic correlation is performed on 
prostate resections?



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Re: [Histonet] Bleach Regs

[Kurth Virginia L. via Histonet]

I agree!


Ginny Kurth

Developmental specialist

University Hospital of Wisconsin

Surgical Pathology



From: Gareth Davis via Histonet 
Sent: Thursday, January 31, 2019 11:52:24 AM
To: Maryann Deathridge
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bleach Regs

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Hi Maryann,
Nosy Med techs.  I have bleach under my sink and never had a CAP inspector
say anything about it being there.


On Thu, Jan 31, 2019 at 8:31 AM Maryann Deathridge via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonetters!
> Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial
> bleach stored under the laboratory sink.  My lab routinely has a gallon
> container of dated/ bio-labeled commercial bleach stored under the sink for
> cleaning purposes. Daily use to clean special stain coplin jars,etc..  I
> had a suspicious med tech tell my pathologist that is was against
> regulations to have it in the lab anytime !
> HUH
> Responses welcomed.
> Have an awesome Thursday!
>
>
>
> Maryann  Deathridge, Lab Manager
> Pathology Assoc. of  St. Thomas
> 4220 Harding  Pike
> Bldg. SE,  Suite 504
> Nashville, TN  37205
> madeathri...@pastnashville.com
>
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--
*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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[Histonet] REPEATS IN IHC

[Kurth Virginia L. via Histonet]

Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit 
on how many times one would repeat a stain; for example but not
Limited too:  The tissue fell off, negative control, wrong block,etc.. for IHC 
DAB and/or ISH.  Thanks Ginny
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[Histonet] HPV ISH

[Kurth Virginia L. via Histonet]

Hello
I have seen question for KDL asking about HPV ISH (awhile back).   My lab would 
like to bring on the HPV ISH using the RNA ISH probes provided by Ventana using 
the silver detection, are you on board using this process for HPV, do you have 
any insight for me.
Thanks!  Ginny, UWHC-Wisconsin, vku...@uwhealth.org
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[Histonet] Silver ISH

[Kurth Virginia L. via Histonet]

Hello Jacqueline
I am going thru old histo net messages and was wondering if you had any new 
insight on the silver ISH for HPV testing on the Ventana ultras, my lab would 
like to validate it, but I am worried about the consistency of the silver 
staining.
Thank you, Ginny Kurth, UWHC, vku...@uwhealth.org
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[Histonet] Her2 dual ISH

[Kurth Virginia L. via Histonet]

Hello
I was reading the past posts of inconsistent her2 dual ISH results on the VMS 
Ultra.  I am wondering if anyone has unlocked the secret of getting consistent 
results?
Thank you advance!  Ginny
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[Histonet] PROX1

[Kurth Virginia L. via Histonet]

Hello
Does anyone send out for Prox1? Thanks.
Ginny
UW-madison
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Re: [Histonet] LFABP and PHH3 Abs

[Kurth Virginia L. via Histonet]

Hi Kiranjit
I saw a question you had out on histonet back in 2015 about LFABP antibody, did 
anyone respond?  One of our doctors want to bring this antibody on.

Thanks
Ginny
UW Hospital of WI
vku...@uwhealth.org
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[Histonet] PLA 2R

[Kurth Virginia L. via Histonet]

Hello
Does or will anyone perform PLA 2R on paraffin  Renal biopsies for validation 
studies.  We are currently sending them
to a reference lab but they will not perform validation slides.  Thanks ahead 
of time.

Ginny Kurth
University Hospital of Wisconsin
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[Histonet] Stat Specimens

[Kurth Virginia L. via Histonet]

What types of specimens do other institutions deem as appropriate for STAT 
processing?
Currently our institution allows biopsies for rejection, graft Vs host, r/o BK 
or CMV.
  Thanks for any input!
Jenny
University Hospital of Wisconsin
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Re: [Histonet] ATRX Antibody

[Kurth Virginia L. via Histonet]

Hi Pam
I was wondering if any reached out to you about ATRX?  Our neuropathologist is 
asking about it.

Thanks

Ginny Kurth
vku...@uwhealth.org
Surgical Pathology
Developmental Specialist
University Hospital Wisconsin

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[Histonet] ATRX Ab Recommendation

[Kurth Virginia L. via Histonet]

Hi Dave

Did you ever receive any information on the antibody ATRX?  Our 
neuropathologist is interested.

Thanks

Ginny Kurth
vku...@uwhealth.org
Surgical Pathology
Developmental Specialist
University Hospital Wisconsin

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