[Histonet] please remove me from Histonet

[McAnn, Sherrian via Histonet]

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RE: [EXTERNAL] [Histonet] GMS controls?

[McAnn, Sherrian]

I have experimented on some things and found Blue Cheese to be excellent source 
of not only fungus but yeast and some bacteria.   I did make a couple of smears 
with the blue cheese but seemed to do much better after just routine processing 
some of the crumbles of blue cheese in cassettes.
Sincerely, 
Sherrian McAnn

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, March 17, 2015 5:51 PM
To: HistoNet
Subject: [EXTERNAL] [Histonet] GMS controls?

Good Afternoon Netters,

I have a project to validate a special stainer and will be starting with GMS.  
What can I use as a good source of fungus?  Was it last week there was mention 
of orange peels and onions?

I need to get the OK of the attending pathologist, so I probably can't use 
anything too funky, like fruits and vegetables.

I don't have access to fresh lung tissue to smear cream cheese on (also 
mentioned last week) so I was hoping some type of easy to buy processed food 
(like Slim Jims from Gram stain) is out there to be used as a fungal control.

Ideas?

Thanks in advance.

Paula :-)
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RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI

[McAnn, Sherrian]

We routinely recycle both our alcohols and xylenes. They are checked for
purity and with the alcohol the extra step of ensuring that we are
getting the correct percentage (95%) recovered. We have never had any
issues in any of our processors or stainers since using recycled
reagents. We also have not had an issue with fumes. The recyclers
nowadays are much better than their older versions and I think that
sometimes prejudices come into play with the older techs like me who
were around for the older models.  P. S. We used to have to do ours on a
hotplate with a large round glass ball and would have to clean the ball
out.  Those were not the good ole days. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Thursday, June 26, 2014 9:43 AM
To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI

I agree with Tom.  With the exception of self-inflicted issues we also
have not had any issues with recycling our reagents.  We check each
batch as it is recycled.  
We also don't have a problem with fumes.  (And our pathologists are
fussy)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Thursday, June 26, 2014 10:34 AM
To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID);
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not? NO PHI

We have never had an issue with either our recycled xylene or alcohol
that was not self inflicted. When our system is running there are no
fumes. 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara
Tibbs
Sent: Thursday, June 26, 2014 9:06 AM
To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not?

While I can agree that recycling alcohol and xylene is both
environmentally and economically advantageous, technically it's awful.
There's no way to make used alcohol and xylene as pure as it was
originally.  There's also the issue of fumes from recycling a solvent.
The company I had used years ago swore that there were no fumes when
using their machine but the personnel working in the laboratory would
vigorously disagree. 

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu
 on behalf of Sanders,
Jeanine (CDC/OID/NCEZID) 
Sent: Thursday, June 26, 2014 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycled or not?

Morning All!

I have heard for years the general problems with using recycled alcohols
on H&E stainers, but do the same problems occur when using recycled
xylene?

Thanks!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology
Branch
404-639-3590
j...@cdc.gov


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RE: [EXTERNAL] [Histonet] Blocks cracking

[McAnn, Sherrian]

We too are having that problem.  I had heard that the paraffin had been
changed and now we get cracks.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erica
Canal
Sent: Wednesday, February 05, 2014 5:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Blocks cracking

Hello all, 
We are wondering harder paraffin makes blocks more fragile.
We have been using Leica's Formula R and on a few occasions & when we
have mailed blocks out, we receive them back in pieces. Could this be
because of type of wax we are using? The quality of our slides is great,
we just seem to be having a problem lately with our blocks breaking
after they have been sent out & we want to try to prevent that.

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RE: [EXTERNAL] [Histonet] VA LIS

[McAnn, Sherrian]

It's true and I do know that we are currently in process of upgrading to
barcode reading ability. I don't know if this applies to all areas of
our hospital but have heard rumors that it is happening.  It's just
seems to be a slow process. LOL

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Simms-Colon, Janine
Sent: Monday, February 10, 2014 9:38 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] [Histonet] VA LIS

Good morning all,

To those working in the VA system, what LIS are you using? We are
currently accessioning through Vista which if you work in the VA is used
for many other processes such as timekeeping and ordering supplies but
it is extremely archaic and not ideal for anatomic pathology, (in my
humble opinion). Our barcoding is not interfaced with Vista and I am
trying to convince management to upgrade to COPATH, WINDOWPATH, or
POWERPATH, really anything other than what we have now. I have been told
all VA Histology labs only use VISTA and I hope to God this is not true.
So if anyone works at a VA, used to work there or know someone who works
there, please contact me to let me know what you are using and how your
process works. Thank you in advance.
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RE: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides

[McAnn, Sherrian]

We once had this problem..We routinely filter our hematoxylin every
morning at startup.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burrage,
Shannon L.
Sent: Tuesday, February 11, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides

Are you using acid alcohol, and Harris Hematoxylin? Are you filtering
the heamatatoxylin every day? We still use a very small amount of
gelatin in the waerbath. And get no background stain. So many veriables
here.
Good luck,
Shannon Burrage
shannon.burr...@unitypoint.org


From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Campbell, Tasha
M. [tmcampb...@fmh.org]
Sent: Monday, February 10, 2014 7:37 PM
To: Huggins, Haley - MRMC
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Purple haze on H&E slides

What brand of reagents are u using and what is your protocol? What
slides r u using and r u using gelatin in ur waterbath?

Tasha

Sent from my iPhone

> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC"
 wrote:
>
> I am trying to get the histology lab up and running and I am having
difficulties getting the H&E Stain just right. I am nearly there, but I
keep getting a purple bleed or haze around the tissue. Any suggestions
on getting rid of this? I actually got rid of it at one point, but the
medical director wasn't happy with the stain. I appreciate any
suggestions from the histology world.
>
> Haley Huggins
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RE: [Histonet] Issue with Henry's Wonder Wax Enhanced

[McAnn, Sherrian]

 Sounds like it may be static...try giving the blocks a little water .
Put a little water in a small container on your cold plate and either
dip a gauze into the water and pat the block  just before cutting (
while in the chuck on the microtome) or place the block directly into
the cold water for a few sec./min. before putting on the chuck to cut.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sandra
Cheasty
Sent: Monday, November 25, 2013 9:59 AM
To: histonet@lists.utsouthwestern.edu
Cc: lca...@svm.vetmed.wisc.edu; langet...@svm.vetmed.wisc.edu; Barbara J
Reese
Subject: [Histonet] Issue with Henry's Wonder Wax Enhanced

Hello fellow Techs,

Has anyone noticed a change in the Henry's Wonder Wax,
Enhanced? (Used for embedding, not infiltrating.) We have noticed that
it is a lot "stickier" when we are sectioning. (To our picks, forceps,
and fingers.) We have checked our temperatures, and all is well. I have
asked the company, but they have not replied. 

Can anyone recommend another embedding paraffin? (We
switched from Paraplast Plus about a year ago when we noticed changes
after the original manufacturer was bought out.)

 

Thank you, and have a safe food and family filled Thanksgiving!

Sandy

 

Sandra Cheasty

Histology & Necropsy Supervisor

UW-Madison, School of Veterinary Medicine

 

 



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RE: [Histonet] Nails falling off slides

[McAnn, Sherrian]

What works for me is this.First I face the block (we don't use any
prepared softeners just regular processing) and then put the block in
ammonia water ( 1%) in a small container on my coldplate and let it soak
for a few minutes. I usually try not to go over 5 minutes but have gone
over that once or twice without problems. I then rinse about 10- 15
seconds.  I place the block back on my cold plate...allow it to get
really cold. I cut at 2.5 - 3 microns. USE POSITIVELY CHARGED SLIDES.
Once you cut the slides...(I usually cut about 3-6 slides so that I can
choose the best ones) put them in an oven for some time to DRY
THOROUGHLY.  We usually let them dry overnight for the best results .
We pick the  best ones the next day for H&E and PASF. Now... sometimes
we still have them wash off, but most of the time all or part stays on
at least a few slides.  I have been in this business for at least 25
years and this is an old method that we used "back in the day". It isn't
perfect but does seem to get pretty good results.  I wouldn't use  less
than pos. charged slides though. Hope that helps youP.S. We have to
let the pathologists know that they  have to be patient when it comes to
nails or else they will get washed off slides.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Friday, November 22, 2013 7:48 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Nails falling off slides

Hi All,
I know this has been mentioned a few times but need some help with nails
falling off charged slides for H&E and PAS stains. I use Statlab nail
prep softener and also use the good charged slides. I heat them 2 times
for 25 minutes each. Is there an adhesive I can put on slide? Albumin?
Any help would be much appreciated, thanks in advance..

Jill Cox HT, (ASCP)
Histology Manager
Arrowhead Dermatology
7747 W Deer Valley Rd Ste 250
Peoria AZ 85382
480-257-7222


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RE: [Histonet] Biopsy Problems

[McAnn, Sherrian]

Seems as though they may  be lifting off in spots? Or maybe perhaps
vibration?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Wednesday, November 06, 2013 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biopsy Problems


Hi Fellow Histonetters, we have started having this problem with our
G.I. biopsies, and not all of them, just the odd one here and there.
After staining, when looking at it on the scope, you can't get it to
focus in one plane.  It's not cutting artifact, because we have recut
the block making sure it is well cooled etc. before picking up and
staining the section.  The cells, when in focus, look fixed and properly
processed, and the staining is the way it should be.  I have no idea
what is causing this artifact.  Has anyone else seen this and can shed
some light on what is causing it?

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico




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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

AMEN TO THAT!

-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu] 
Sent: Friday, October 25, 2013 10:05 AM
To: McAnn, Sherrian; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

The rule here is" a blade is cheaper than a cut".  Anytime you are
walking away and returning within a few minutes to cut use the knife
guard otherwise throw the blade out.  It is an accident looking to
happen.  Recently we had a tech decide not use the knife guard and
seriously cut himself by misjudging the distance from his elbow reaching
an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn,
Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the "safety guard" up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

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On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
wrote:

> Hello all :-)
> I am doing a quick microtome blade safety survey, When you finish 
> work, do you leave your blade in the microtome behind the blade guard 
> or do you take it out?
> If you take it out and it is a new blade or a blade still useful for 
> trimming  where do you store it?
> Thank you for your feedback, I really appreciate it.
> Regards
> Leah Simmons
>
> ___
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>
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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the "safety guard" up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents
are covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended
recipient or an agent responsible for delivering it to the intended
recipient, you are hereby notified that you have received this document
in error and that any review, dissemination, copying or the taking of
any action based on the contents of this information is strictly
prohibited . If you have received this communication in error, please
notify us immediately by e-mail, and delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
wrote:

> Hello all :-)
> I am doing a quick microtome blade safety survey, When you finish 
> work, do you leave your blade in the microtome behind the blade guard 
> or do you take it out?
> If you take it out and it is a new blade or a blade still useful for 
> trimming  where do you store it?
> Thank you for your feedback, I really appreciate it.
> Regards
> Leah Simmons
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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RE: [Histonet] Tissue Tek VIP 6 and contaminated alcohol

[McAnn, Sherrian]

Almost sounds as though contaminated with xylene...you may need to have
a professional come out and do a pm and then make sure that it gets
proper daily , weekly and monthly maintenance done. We had the same
problem too and found that some people were not doing proper
maintenance.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria
Samaan
Sent: Wednesday, August 14, 2013 11:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Tek VIP 6 and contaminated alcohol

The 100% alcohol on our processor has been contaminated lately and I
have been unable to pinpoint the cause.

Our processor recently had a PM and checked out ok.

The alcohol was cloudy when tested with water, which is how we normally
test for purity before/after the recycler.  I was told by the
manufacturer that I should check the alcohol with a hydrometer, the used
100% alcohol from the processor was over 100% (which I have never seen
before).  I also checked a new bottle of alcohol and it also read over
100% so then I wondered if the hydrometer is calibrated only to a
specific alcohol or if it has been damaged.

So far our tissue seems to be processing fine but I would like to fix
this before it becomes an issue.

Thank you for any input,
Maria Samaan
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RE: [Histonet] Waterbaths

[McAnn, Sherrian]

Sorry I forgot to say that I like to use dish soap..namely dawn...but
almost any laboratory glassware soap would probably do the trick.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M.
Murphy
Sent: Monday, August 05, 2013 12:02 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waterbaths

Just wondering if everyone leaves the distilled water in their water
baths overnight or replaces the water first thing in the morning?  Also,
what do you use to clean your water baths?

LeAnn Murphy
Technical Specialist Aultman Hospital
lmurp...@aultman.com

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RE: [Histonet] RE: Waterbaths

[McAnn, Sherrian]

I  wash mine with hot soapy water and a soft green scrubby to remove the
day's paraffin and bacteria (I'm sure it grows some).  Then squirt a
little absolute in it and wipe around to help dry it .  We have a
morning person who fills all water baths every morning. Personally I
don't like the idea of letting water sit overnight as I feel that dust
can fall into it.  Also I feel like it could be a good  breeding ground
for bacteria. This is just my personal opinion

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Monday, August 05, 2013 12:05 PM
To: Leann M. Murphy; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Waterbaths

I empty mine each day and dry it. Mine does not have a glass insert or I
would run it through the dishwasher so instead I wash it every few days
with warm, soapy water.

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology
Branch
404-639-3590
j...@cdc.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M.
Murphy
Sent: Monday, August 05, 2013 1:02 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waterbaths

Just wondering if everyone leaves the distilled water in their water
baths overnight or replaces the water first thing in the morning?  Also,
what do you use to clean your water baths?

LeAnn Murphy
Technical Specialist Aultman Hospital
lmurp...@aultman.com

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RE: [Histonet] Recycled Xylene

[McAnn, Sherrian]

We do our own recycling of alcohol and xylene.  W e use the recycled
xylene for everything including cleaning the processors.. No
problems..been using it for years

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Monday, July 29, 2013 10:47 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Recycled Xylene

Does anyone out there have any problems with using recycled xylene to
clean the tissue processor?   I was just told that we should not use
recycled xylene to clean the tissue processor.  How about using recycled
xylene for either processing or staining?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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RE: [Histonet] Fatty Fixation

[McAnn, Sherrian]

We use Dissect-Aide seems to work really well especially with lymph
nodes too.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White,
Lisa M.
Sent: Thursday, June 20, 2013 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fatty Fixation

Does anyone have a method they will share to fix fatty specimens?  Does
anyone utilize a stir plate?  Any help greatly appreciated.  

We currently use Alcoholic Formalin but the results are not reliable.

 

 

Lisa White HT(ASCP)

Supervisory HT

James H. Quillen VAMC

Corner of Veterans Way and Lamont

VAMC Warehouse BLDG. 205

PO Box 4000
PLMS 113

Mountain Home, TN 37684

423-979-3567

423-979-3401 fax

 

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RE: [Histonet] Cutting paraffin sections on a cryostat operated at roomtemperature? Nope.

[McAnn, Sherrian]

Thank You!  If you have ever had to break one down and clean and put it
back together you would know it is difficult (at best) to even turn the
handle. These are precisely machined and don't operate well ...if at all
when warm. I suppose though that you could cut your sections cold ,
however seems like it would be awkward.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Harrison, Sandra C.
Sent: Monday, June 17, 2013 8:30 AM
To: Johnson, Kevin; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cutting paraffin sections on a cryostat operated at
roomtemperature? Nope.

Ever tried turning the handle of the cryostat, when it's at room
temperature?
Cryostats are tooled & manufactured to operate at a low temperature.
Since metal contracts at the low temperature, you'll find that you can't
operate the microtome at the higher temperature.  The handle will barely
move.  

Sandy Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VAHCS
612-467-2449

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson,
Kevin
Sent: Friday, June 14, 2013 3:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cutting paraffin sections...on a cryostat?

Hi, all.  A bit of an odd question: a colleague knows of someone wanting
to cut paraffin sections who has a cryostat, but no microtome. Since a
cryostat's basically a microtome in a freezer chamber, I thought that it
may be awkward, but theoretically doable once it was brought to room
temp and dried out thoroughly. However, I wondered if lubricants
formulated for the cold might become too thin for use at room temp,
possibly causing damage to moving parts.  Any thoughts?

Kevin Johnson
University of Miami
Diabetes Research Institute
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RE: [Histonet] Mohs histology hourly rate

[McAnn, Sherrian]

Here in San Antonio, Texas..I hear it is a lot more than that .  Maybe it is a 
supply and demand thing.. starting wages for a bench Histology Tech here can 
run between 19.00 and 21.00 dollars.  I have heard that it is a lot more for a 
MOHS tech..like maybe around 30.00 dollars and up.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
rook...@comcast.net
Sent: Tuesday, May 14, 2013 8:11 PM
To: .
Subject: [Histonet] Mohs histology hourly rate

Hello, I was wondering if anyone knows what the going rate is for a Mohs 
histologist (Hourly). I have 12 years experience as a histologist doing routine 
histology and just recently got a job doing Mohs histology. Thanks in advance 
for any feed back, Christina ___
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RE: [Histonet] Blades

[McAnn, Sherrian]

 I have been in histology and cutting for about 26 years now.  I have
used many types of blades, high and low profile.  My favorite and I
think the best ones are Surgipath Teflon coated high or low profile
blades.  I believe the high profile blades are the best but I do realize
that not everyone has that option. I think if you tried them you
wouldn't be disappointed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hale,
Meredith
Sent: Monday, April 15, 2013 12:39 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades

I would like some feedback from you on the types of blades those of you
who cut GI biopsies prefer ? Do you see differences with chatter on
different blades. Any feedback is appreciated . Thanks !

Meredith Hale HT  (ASCP)cm
Director External Sales Support

Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mh...@miracals.com>

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[Histonet] (no subject)

[McAnn, Sherrian]

I agree and would like to add.  This is one scenario that I have seen
many times, where hospitals  or wherever will hire histotechs without
certifications .  I am thinking that saves them money and they still
have a "histotech".  I have seen good histotechs  that have no
certification and likewise some bad ones with certification.  Lately I
have seen these  schools turn out histotechs   ,  it seems with little
encouragement to get certified.  If places will hire them without being
certified,  there seems little incentive (unless you are self motivated
for more money) to move on up to certification.

 

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