[Histonet] Equipment available to the highest bidder
Hi list, On 21 of February we will have the following equipment available to the highest bidder: 1. Tissue processor - Tissue Tek VIP-E (still in use) 2. Tissue processor - SHANDON Citadel 2000 One paraffin tank missing 3. JUNG AUTOSTAINER LX (still in use) By the end of the month we will also have available 4. Embedding center - SHANDON Histocentre2 (still in use) If interested please send me an offer. Michelle Aloni MS HTL Research Specialist USC Keck School of Medicine Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For H&E staining - TBS SHUR/Stain or Leica Autostainer XL
I have and love my old Autostainer XL. Now we are offered a new TBS SHUR/Stain unit, which is attractively small, but I have never used nor seen it in person. We are a small University based lab and our space is limited. We rarely run few racks at a time. However, we must be able to run only deparaffination and only dehydration for our immunos. If any one has experience with the TBS unit, or even better, used both of them, please let me know what do you think. I would appreciate this very much Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Research and Clinical Labs
For 10 years I ran Histology lab for a large University. We used the same equipment and lab for both animal and human tissue. I had to certify the lab with CLEA for human tissue at one point (and this took serious work!). If you are already certified for human, using the more strict regulations which apply to human tissue, won't hurt the rest, nor increase in any way your time or effort involvement. You do it anyway. I wouldn't leave anything marked "for research" unless it has proper label with exp date etc. We received everything already fixed and most of the time in cassettes, so there was not much grossing. The processor ran the same night and everything was finished the following day. No one, during any of the inspections had trouble with this. As far as I remember, there was no question about this in any of the check lists sent to me. It was 11 years ago when I parted with my Histo lab and I am not current on the latest regulations, but as far as the technical part... there shouldn't be a problem. Michelle Aloni MS, HTL ASCP USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Measure productivity by skill level
THANKS! It worked J Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Measure productivity by skill level
I would also be interested to know that. I am not even familiar with Welcan units. Would someone please elaborate? Michelle Aloni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need to compare prices
Hi all, We do Histology and Immunology primarily on liver tissue and only for research. I need to check the prices of other cores and present numbers for comparison as soon as possible. I remember that some time ago there was a question about prices for Histology in a research environment. Most of the responses, however, must have been directed to a personal mail. I would be very thankful if the person who did this research (already) gets in touch with me ( macve...@usc.edu or 323 442-1188). At the same time, I would appreciate very much all the information that anyone of you can share with me. You can just scan and e-mail your pricelists if this is easier, and please mention the city and state of your location. If you would rather fax it, please write back and I will give you our fax number. Have a nice day and thank you ahead of time Michelle Aloni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sirius red stain - picric acid substitute
Hi Mia, I tried few different sources of Picric acid. Unfortunately, not all of them worked. The one that is working is from Rowley Biochemical Institute - www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You can probably find it from one of the large vendors. Just a helpful thought Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O for FS on Muscle
Few more things: 1. The mixed/working solution is good for 1-2 hours only. If you filter it for a long time, this might use the good working time and then you have trouble, because it just won't work well for you. I just poor the stain and the water in a glass cylinder and shake it. That's it. 2. After coverslipping, do not press on the coverslip to chase air bubbles away or this might displace the lipid droplets from their original place. 3. Look at the slides as soon as possible. In few days, you will see that the stain is changing into ugly black crystals and the longer you wait the larger the crystals = have to repeat it all over again J 4. To clean the glassware, spray All Purpose Cleaner, use a brush and warm water Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O for FS on Muscle
Hi Akemi, Order the stain from Electron Microscopy Sciences - cat # 26503-02. It comes as 250 ml solution and is a lot less expensive than some other sources. I guess you can probably get it through VWR. Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10 min. Filter through coarse filter paper. I have even used paper towel with no problem. Anything finer will take forever to filter. Fix the sections with regular 10% NBF for few min. 3-5 Rinse slides in dist. Water few times Working Oil -red-O for 10 min. I use the plastic containers for shipping slides, because the stain will color the plastic and I can easily and cheaply replace those, but you can wash them and reuse them multiple times. 70% alcohol - quick rinse to clear background Dist. Water - 5 min Mayers Hematoxylin 5 min Tap water 10 min Dist. water rinse or up to 1 min Mount with Aqua Mount Never had any problems. Normal muscle will have very, very tiny droplets. We used it on rodent muscle, but it should work the same for human. Good luck Michelle Aloni Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin
Absolutely not!!! In California. Safety office picks it up and dispose of it. Michelle Aloni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O control
Once you mix your stock solution with water, it is good for only about an hour. Then you have to discard it. Michelle Aloni MS HTL (ASCP) USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need maintenance contract in LA
Hi all, We have a very small University lab. We don't work with human tissue, so we don't actually need to show record of maintenance. We would like to cover our histology equipment with a maintenance contract just in case because it is mostly old. Does someone in LA area offer maintenance for histo equipment and how much would this be. Beside all the old stuff we also have a new Leica Bond, which we would like to cover with someone different then Leica. Please contact me off the list. Michelle Aloni Research Specialist macve...@usc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histomorphometry
We use MetaMorph software. It comes with a digital camera for taking the pictures. You can get the software only if you have already the micrioscope and the camera. You can easily measure anything you want, but it is costly. Michelle USC School of Medicine LA CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HM 500 M cryostat
Hi Andrew, I worked on different cryostats and I have memories of chuck going up. The section is cold and sturdy and I would just let it follow the chuck up as it is still attached to it. Then pull down, touch it to the knife so it sits there (it gets attached at the bottom) and pick it up. It is a bit annoying, but you get used to it. In your case, you will pay a fortune for service with no guarantee that the unit will work the way you want it. Who knows what lese will go wrong in the process... Michelle Aloni USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is anybody using TBS ATP1 - 120 tissue processor?
Hi all, Would you please let me know off line what do you think about this unit? macve...@usc.edu Thank you very much in advance Michelle Aloni MS HTL ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology stainers
Hi histoneters, We have an old Jung Autostainer XL, which has been great, but it is very old and on its way out. What kind of newer stainers are you guys using? Should I even look at something diferent? I also would like to hear from the people using Exelsior tissue processor. Do you guys like it and how does it compare to the good old VIP? Michelle Aloni MS HTL ASCP Research Specialist USC Keck School of Medicine LA, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TUNEL
We also use Roche kits. The cat # 11 684 795 910 is for fluorescent and cat# 11 684 817 910 is for loight microscope. There are aditional chemicals to purchase, beside the kit. Call Roche for more info. Good luck! Michelle USC School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How do I subscribe?
Hi all, Can someone tell me - how does one subscribe to Master Tech Flyer? Thank you in advance Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mouse kidney frozen sectioning
Hi Andrew, You must freeze fast to avoid the needle like crystals forming from the water during freezing. Best - freeze in OCT in liquid Nitrogen. It gets worse if you keep changing the temp of the block. For example keep at -80 then bring to -20 cut than bring the temp down to remove the block from the chuck and put back at -80. Few of those will make your tissue hard to even recognize. Good luck Michelle HTL USC Keck School of Medicine LA, CA - Original Message - From: "Andrew Burgeson" To: Sent: Monday, May 03, 2010 12:31 PM Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does anyone have and use Shandon Excelsior EX
Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Liver Tissue
Soak the faced block in room temp. water for few minutes. This will speed the intake of water. I can usually cut it at room temp if I am careful. If your paraffin is soft then you will need to soak it in room temp water first and then put ice on it and cut it. Pick up the ribbon and place on room temp dist water. While you are picking up and stretching the sections, put piece of cotton soaked in water on the face of the block as it is attached to the microtome. This way it will be soaking in place and you won't be loosing nice sections while adjusting the angle for the next ribbon. By the time you pick up all good sections, the block will be ready to be cut again. If you have good size liver, you can soak it for much longer - 5-10 min, before you start sectioning. This way the water will penetrate dipper and you will be able to easily get a very long ribbon. Good luck Michelle Aloni BS HTL Research specialist USC Keck School of Medicine, LA CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Licensing for Massachusetts?
Hi all, I live in Los Angeles and have HT and HTL from CA. I would like to move to Cape Cod in couple of years. I know that one needs a special Florida license to work in Fl, but do I need a special license from Massachusetts to be able to do histology there? I would appreciate any info Michelle Aloni USC Keck School of Medicine Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Frozen Myocardium sections
The OCT cracks if the block remains in liquid N too long. I use a plastic mold, which holds my tissue in the OCT. Float the mold on the surface of the liquid N but pull it out of there while there is still a little liquid/clear OCT (about 6-7mm in diameter) in the middle of the forming block. Put it on the counter (room temp.). As the block sits on the counter, for a minute or two, the freezing of the center takes place. The block will never crack if it is pulled out of the OCT on time. Michelle Aloni MS HTL ASCP USC Keck School of Medicine Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Overstained Sirius Red...
Hi everybody, I have been doing Sirius Red for a while with good luck. Today we made fresh solutions and after the stain was done, the sections (rat liver) are too red. They are positive, the fibrosis is bright pink, but the background is pink as well. I got a slide back to water and distained for 2 more minutes with no luck. The only difference is the source of the saturated picric acid. On the old bottle the manufacturer is listed as Rowley Biochemical Institute (worked great) and the new one is Ricca Chemical CO. Could this be the problem? What can I do to correct this in the future? I have now 500ml of stain which is not optimal. Michelle Aloni USC School of Medicine GI Liver Department LA, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: DAB and Nuclear staining
Hi Melissa, We use Mayer's hematoxylin for 5 min and then rinse in tap water for at least 5 min (to blue the stain). If your DAB stain is weak, you can counterstain for even shorter time. It works great! The other option would be 1% Methil Green in 0.1M Sodium Acetate. My Methil powder is extremely old and probably this is the reason the stain is very weak, but it makes great contrast with the brown of the DAB. Good luck Michelle - Original Message - From: "Melissa Mazan" To: Sent: Thursday, September 03, 2009 6:18 AM Subject: [Histonet] DAB and nuclear staining > Hi all, > I have been having a problem with counterstaining for nuclei - I'm > looking at mouse lung tissues and staining macrophages with F4/80 (rat > anti-mouse). I use a negative serum control and a rat IgG isotype > control. I see very clear staining of macrophages - but when I > counterstain with hematoxylin or nuclear red, everything becomes muddy > and I can no longer discern the DAB staining. Can anyone offer advice? I > am using the Vector hematoxylin, and have tried as little as 5 seconds. > Melissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What do you use for decolorizing after Hematox.
Hi all, In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it? What do you use in your regulat H&E stain? I am using it on an old Jung AUTOSTAINER. Michelle Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: working with mouse liver
Hi Teri, We have been discussing this option and left it at that. I have noticed that the livers, with more lipids look much closer to the textbook images. Michelle, Could it be what you are seeing is normal healthy (actively feeding) liver in mice and the missing cytoplasm is where the glycogen stores are/were? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] working with mouse liver...
Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for different length of time with no luck. The processing time is 1 hour in every station. Did anybody working with liver has any suggestions? Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: from PLEASE HELP
Hi Delia, Some time ago I used to work in a lab where I had to cut skin punch biopsies. They used to come floating in a special preservative. Unfortunately I don't remember its name, but then I had to wash them in a special wash solution for a minute. The wash solution was made by Zeus Scientific and was just citrate buffer. I am not familiar with the Michael's fixative and don't know do you have to wash after fixing. The trick was to pat dry the biopsies with Kimwipes and immerse them right away in the OCT. Then let the biopsy sit there for few minutes. You can even move the tissue in the OCT from time to time and then transfer it to a new mold with fresh OCT. Freeze in liquid Nitrogen. This way, somehow the OCT starts infiltrating the tissue and the tissue cuts together with the OCT. I used to cut it at 5 microns. Hope that this will work for you too Michelle Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Poly Slides
Hi Diane, I used poly coated slydes (which we made in our lab years ago to save money). Then the plus slides came along but are pricey. I just tested and liked a new source for my plus slides which are extremely well priced and worked just like the FISHER and VWR slides... only for half the price :-) Try www.LabMed.biz or call LabMed 877 504-7960 Michelle Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Immunocytochemistry for Systems biology
Hi Vinay, I am not an expert in this field... We use this protocol for frozen sections and it works. For cells grown on coverslips you skip step #6. Try this : Fix 1. PBS 2 x 3min 2. NH4Cl 5 min (50mM in PBS) (Removes the autofluorescence from the aldehyde groups of the fixative) 3. PBS 2 x 3 min 4. 0.1% Tx-100 5 min (Permeabilises the cell walls) 5. PBS 2 x 3 min 6. SDS 5 min (1% SDS in PBS) (works like antigen retrieval) 7. PBS 3 x 5 min 8. Block 1% BSA in PBS10 min or longer 9. Primary Ab40min in 37oC or 1 to 2 hours at room temp 10. PBS 2 x 5 min 11. Secondary Ab 40min in 37oC 12. PBS 2 x 5 min 13. Coverslip with Anti fade medium Let dry overnight Good luck with it Michelle Research specialist USC Kecl School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Citadel Tissue Processor
Hi Atoska, We do only research and have low volume. We purchased a second hand one and used it for about a year. It works and does the job. It is much better than having to process manually but: The alcohols evaporate like crazy! You loose a lot. It must be in a hood. There is no heating nor vacuum. I was under the impression that there is vacuum while reading the brochure, but when the Citadel arrived, I found that it is a separate free standing unit that can be connected only with the paraffin tanks. We didn't have the room for it so we never used it and sent it back. There is no purge/clean cycle. You have to either purchase 2 extra buckets or find some other containers for the Xylene and the 100% which you will be using to manually wash the basket after the cassettes are out of it. They should also be in the hood. In a year, one of the paraffin buckets burned. The service guy told me that a repair was not worth it. A new paraffin bath is about $1200.00. Something that was good is that you only need about 1/2 gallon per station. When we had only few cassettes, we didn't even fill completely the buckets. Infiltration was fine. Hope this helps Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fat histology
Hi Karen, I had the same problem recently. I got very good advise trough the histonet for working with fat. You might want to check the arhives. In my case, I was in a big time crunch with this dog fat, so after all the fat was collected and stored for a while in 10% NBF I did'nt have any time to post fix it, I just had to put it in the processor. It came out great! I used 1 hour in all steps and the steps are: 1Formalin1 hour 280% 1 hour 395% 1 hour 495% 1 hour 5100%1 hour 6100%1 hour 7100 % 1 hour 8Clear Rite 1 hour 9Clear Rite 1 hour 10 Clear Rite 1 hour 11 Paraffin 1 hour 12 Paraffin 1 hour 13 Paraffin 1 hour 14 Paraffin 1 hour I did it again few days ago (the same way) and again had great result. We also did IF on this fat and it worked great as well. Good luck with it Michelle Research Specialist USC Keck School of Medicine Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Working with fat
Thank you very much! This sounds perfect! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Working with fat
Hi Cheri, Thank you very much for the formula. I will give it a try. Do you just put the fat into it or it is a post fix? After the hour into this solution, what do you do? Do you move it to NBF or 70% ETOH until you start the processor? How long can the fat stay in this fixative? This is a research project and it is just fat that this group is looking at. Thank you for your help Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Working with fat
Hi all, I used to postfix in PenFix the fat specimens and had good luck. This time we don't have any PenFix :-( The fat is floating in 10% NBF. What can I do to help the fixation, so I can infiltrate well enough, so I can cut the fat and get good morphology? Any suggestion is welcome Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome vibration:
Hi Ana, First, I would remove the knife and the knife holder and clean everything from even the smallest debris. After it is all spotless, I would put it together, make sure all screws are tight and try it again. More likely this will solve the problem. Second - look carefully at the knife holder. We have had a problem with a notched knife holder that had to be polished patiently with fine sand paper for metal (on flat surface) until we removed the damage/notch from it and got it to be very smooth again. Hope this helps Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond Max - any opinions?
Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glicerol jelly with anti-fading agent
We use the ProLong Antifade Kit (P7481) from Molecular probes. It comes as: 1. Powdered ProLong antifade reagent (Component A) 20 vials of powder. 2. ProLong mounting medium (Component B) in 2 bottles - 15 mL each. 1mL of component B should be mixed well in a vial of powder. This should be used in 24 hours. It works great. Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need any info for JUNG HISTOSTAINER Ig
Hi all, We do research. We got a hand me down JUNG AUTOSTAINER XL which now does all the H&E and it is a blessing! Now I am thinking about purchasing a second hand Immunostainer to run some immunos, so we can use our time for something better than babysitting the slides. There is a very inexpensive JUNG HISTOSTAINER Ig available, but I have no experience in this area. (I couldn't even find a picture of it on the Internet...) For now we only do a TUNEL stain for apoptosis, but the need for more immunos is rising. (Our students use a lot of fluorescent markers if this makes any difference.) What do you think? Do you recommend it or am I just investing in a pricey incubation chamber? I would appreciate any input Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
