[Histonet] Slides for Tissue microarrays

[Murphy, Valerie via Histonet]

Does anyone make their own sticky slides for cutting TMA blocks? Is there an 
adhesive substance we could apply to our regular plus slides?


Valerie Ratliff BS HT(ASCP)

Wayne State University


McLaren confidentiality statement: "The information contained in this 
communication, including attachments, is confidential, may be privileged, and 
is intended only for the use of the named recipient(s). Unauthorized use, 
disclosure, forwarding or copying is strictly prohibited and may be unlawful. 
If you have received this communication in error, please notify me IMMEDIATELY 
at the phone number or pager listed above."
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Automated IHC instrument

[Murphy, Valerie via Histonet]

Hello Histonetters,
Our tissue core is interested in purchasing an IHC instrument. It would  be 
used for the more routine assays such as ER/PR, Her2, Ki67 etc.
Can anyone make a recommendation? Leica, Ventana, Dako?

Thank you,

Valerie Ratliff  B.Sc HTL(ASCP)
Research Assistant
Department of Oncology
Karmanos Cancer Institute
4100 John R
Detroit, MI 48201

Telephone: (313) 576 8282
Fax: (313) 576 8306
E-mail: 
murp...@karmanos.org

Better treatments. Better outcomes.


---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Processing human brain xenografts

[Murphy, Valerie via Histonet]

Hello Histonetters,
I was wondering if anyone could offer advice for the  processing of our  human 
brain xenografts harvested from mice. The tumor specimens are approximately 0.5 
x 0.5 x 0.5cm.  We have been putting them on our animal program ( 30 min 
alcohols) but once embedded, the tissue looks brittle and some shrinkage 
occurs. The morphology looks okay. S hould we put them on our routine program ( 
1 hour ethanols and xylenes) or is brain tissue too delicate for that run?
Would appreciate any advice.

Thank you,

Valerie

---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue Processors

[Murphy, Valerie]

We are looking to purchase a new tissue processor for our tissue core. The 
workload is quite light.
Can anyone recommend a small, reliable processor ?

Thank you,

Valerie Ratliff BS HT(ASCP)
Wayne State University
Detroit, Michigan


---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Brain tissue coming off slides

[Murphy, Valerie]

Thank you all for the great tips!
 I will try your suggestions.

Valerie Ratliff
Karmanos Cancer Institute


---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Brain tissue coming off slide

[Murphy, Valerie]

We are staining human FFPE brain sections for Her 2 Neu and we're having a 
problem with tissue coming off the slide during the HIER step.
We are currently using Vectabond coated plus slides and using a decloaking 
chamber for HIER.
Are there any other steps we can take to prevent  tissue coming off the slide?

Thank you,

Valerie Ratliff
Karmanos Cancer Institute


---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: antibody vials

[Murphy, Valerie]

I use cardboard cryovial boxes and they work quite well. You can remove some of 
the dividers to make space for the larger vials.
http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Freezer-Racks/Cryovial-Boxes-Cardboard/




Valerie Ratliff  B.Sc HTL(ASCP)

Research Assistant

Department of Oncology

Karmanos Cancer Institute

4100 John R

Detroit, MI 48201



Telephone: (313) 576 8282

Fax: (313) 576 8306

E-mail: murp...@karmanos.org



Better treatments. Better outcomes.


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Clare Thornton 
[cthorn...@dahlchase.com]
Sent: Tuesday, May 20, 2014 10:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibody vials

Does anyone have any good ideas for keeping the little vials of concentrated 
antibodies neat and organized in the fridge?  Right now we keep ours in empty 
pipette tip boxes (without tip holders) but they're always falling over and 
mixed up.  We use several different vendors for our concentrated antibodies, so 
our vials are all different sizes.  Thanks for any ideas!



Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Processing frozen brain samples

[Murphy, Valerie]

Hi,

I am new to Histonet. Here is my question.

We have brain samples that were fixed in 4% paraformaldehyde then placed in 30% 
sucrose (cryoprotectant) prior to freezing on dry ice. These samples have been 
stored at -80 for a couple of years. We want to thaw these samples then process 
them to make paraffin embedded blocks. What is the best way to remove the 
sucrose prior to processing? Should we just  rinse with PBS?



Thank you,



Valerie



Valerie Ratliff  B.Sc HTL(ASCP)

Research Assistant

Department of Oncology

Karmanos Cancer Institute

4100 John R

Detroit, MI 48201



Telephone: (313) 576 8282

Fax: (313) 576 8306

E-mail: murp...@karmanos.org



Better treatments. Better outcomes.

---
Confidentiality Notice: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and/or 
privileged information. If you are not the intended recipient(s), you are 
hereby notified that any dissemination, unauthorized review, use, disclosure or 
distribution of this email and any materials contained in any attachments is 
prohibited. If you receive this message in error, or are not the intended 
recipient(s), please immediately notify the sender by email and destroy all 
copies of the original message, including attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet