Re: [Histonet] plants in the lab

2015-05-29 Thread Norton, Sally
We have them, but have not documented the benefit, other than our enjoyment of 
them, especially when the violets and orchids bloom!

Sally Norton
Seattle Children's Hospital


-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com]
Sent: Friday, May 29, 2015 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] plants in the lab

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda
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RE: [Histonet] Kidney Stone Histology?

2015-04-03 Thread Norton, Sally
The only thing we do with stones is send them to the Mayo clinic for chemical 
analysis.

Sally Norton
Seattle Children's



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
Sent: Friday, April 03, 2015 11:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kidney Stone Histology?

How the heck do I process and section kidney stones?  And what kind of stain do 
you like for them?


Thanks

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
  
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RE: [EXTERNAL] [Histonet] GMS controls?

2015-03-18 Thread Norton, Sally
We used Brie that we let grow mold for fungus.

Sally Norton
Histo Tech
Seattle children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Wednesday, March 18, 2015 9:58 AM
To: Paula Sicurello; HistoNet
Subject: RE: [EXTERNAL] [Histonet] GMS controls?

I have experimented on some things and found Blue Cheese to be excellent source 
of not only fungus but yeast and some bacteria.   I did make a couple of smears 
with the blue cheese but seemed to do much better after just routine processing 
some of the crumbles of blue cheese in cassettes.
Sincerely,
Sherrian McAnn

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, March 17, 2015 5:51 PM
To: HistoNet
Subject: [EXTERNAL] [Histonet] GMS controls?

Good Afternoon Netters,

I have a project to validate a special stainer and will be starting with GMS.  
What can I use as a good source of fungus?  Was it last week there was mention 
of orange peels and onions?

I need to get the OK of the attending pathologist, so I probably can't use 
anything too funky, like fruits and vegetables.

I don't have access to fresh lung tissue to smear cream cheese on (also 
mentioned last week) so I was hoping some type of easy to buy processed food 
(like Slim Jims from Gram stain) is out there to be used as a fungal control.

Ideas?

Thanks in advance.

Paula :-)
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[Histonet] RE: Mushrooms for GMS fungus control

2015-03-06 Thread Norton, Sally
We have used orange rind and brie - left to grow mold  - as controls in a 
pinch.  Our docs preferred the cheese.

Sally Norton
Seattle Children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Friday, March 06, 2015 9:38 AM
To: 'Piche, Jessica'; Jeffrey Robinson; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Mushrooms for GMS fungus control

As your fungal control?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, March 06, 2015 11:32 AM
To: Jeffrey Robinson; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Mushrooms for GMS fungus control

I will say I hacked a mushroom off of a tree once and processed it and it 
stained nicely for GMS. I also used some chicken that was in the fridge too 
long and we actually use that for our GMS control!!

Jessica Piche, HT(ASCP)
Waterbury Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Friday, March 06, 2015 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mushrooms for GMS fungus control

How about mushrooms?  Has anyone had any success using mushrooms as a GMS 
fungus control?

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA


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[Histonet] RE: biopsy "bags" for processing - alternatives

2014-08-13 Thread Norton, Sally
We wrap our specimens in End Wrap papers (cut in half).  Takes more time I 
suppose than putting them in bags.

Sally Norton, HT
Seattle Childrens Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Wednesday, August 13, 2014 08:37
To: Timothy Morken
Cc: Histonet
Subject: Re: [Histonet] biopsy "bags" for processing - alternatives

Our kidney and liver biopsies are placed in BX bags (tea bags).  The 
pathologist feel the nylon bags leave a pattern on the tissue and sponges are 
even worse.  The Gross Room staff and residents also dislike the nylon bags as 
they feel they are harder to handle and stiff.  Then we in Histology feel 
exactly the same as Tim's description.  We have tried various things and keep 
going bag to "tea bag" style biopsy bags.  If anyone has come up with a better 
idea or product please let us all know.
Thank You,
Pam Marcum
UAMS

- Original Message -

From: "Timothy Morken" 
To: "Histonet" 
Sent: Wednesday, August 13, 2014 10:24:00 AM
Subject: [Histonet] biopsy "bags" for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to "pop" open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.org


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RE: [Histonet] GI biopsy slides

2013-09-10 Thread Norton, Sally
Are your specimens large?  We cut two levels, with two ribbons on each slide.

Sally Norton
Seattle children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, September 10, 2013 08:48
To: 'Elizabeth Chatfield'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] GI biopsy slides

We put 3 levels on the same slide when specimen size allows.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chatfield
Sent: Tuesday, September 10, 2013 11:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biopsy slides

Hi folks,

We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 
levels with 3 sections on each slide.  Is anyone out there putting all 3 levels 
on the same slide?  We are receiving cases with a large number of samples and 
some our pathologists would like to see fewer slides.


Thanks,
Elizabeth
Charlottetown, PE

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RE: [Histonet] Travel Histology Technician Jobs

2013-09-10 Thread Norton, Sally
Hahaha, I'm not interested in speedboats or supermodels!!  I will retire in two 
years and thought about this as something to do for a year or two after.  Since 
I will have social security I can't make too much anyway.  My only 
comment/question regarding Jay's post is - cheap motel?  I've visiting nurses 
whose places don't look like cheap motels.

Sally Norton
Seattle children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jeff lowen
Sent: Tuesday, September 10, 2013 08:11
To: Morken, Timothy; Jay Lundgren
Cc: histonet; Christopher Jacobs
Subject: RE: [Histonet] Travel Histology Technician Jobs

Me too?   umbrella girls and champagne for all

> From: timothy.mor...@ucsfmedctr.org
> To: jaylundg...@gmail.com; lowenj...@hotmail.com
> CC: histonet@lists.utsouthwestern.edu; cjac...@clinpath.com
> Subject: RE: [Histonet] Travel Histology Technician Jobs
> Date: Tue, 10 Sep 2013 15:08:03 +
>
> "  It ain't all speedboats and supermodels, if that's what you had in 
> mind."
>
> Bummer, That's exactly what I had in mind!!
>
>
> Tim
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
> Sent: Monday, September 09, 2013 5:07 PM
> To: jeff lowen
> Cc: histonet; Christopher Jacobs
> Subject: Re: [Histonet] Travel Histology Technician Jobs
>
>  I honestly wouldn't recommend it unless you are financially able to 
> undergo 6 month plus periods of unemployment.  In 2008 I didn't work all 
> year, only maybe the last 3 weeks.  The wages have never come back to the
> pre- 2008 levels. The largest staffing agency in the country declared 
> bankruptcy because of the downturn.
>   In today's economy, there is always going to be an unemployed person 
> who is willing to work for a couple of dollars less, and this affects the 
> agencies as well.  Most of the HR depts I've dealt with lately, ONLY care 
> about cost. As long as the applicant is JCAHO compliant and registered, 
> they'll take the one who is 50 cents cheaper.
>   So, right now, the agencies are able to get people for ridiculously low 
> wages.  I won't be as crass as to post any, but they are basically asking you 
> to subsidize your own working vacation.  Usually in a semi-rural, isolated, 
> or climatically blasted location (there's a reason they can't get a full time 
> tech), living in the cheapest motel in town for
> 3 months.  When you ask the agency why they put you in that particular motel, 
> they reply, "Because it's the cheapest place in town. (DUH)"  Some agencies 
> are better than others, but they are all SUPER cheap.  The better ones are 
> just honest about it.
>   I see full time jobs advertised regionally (Texas) that are offering 
> registered techs  jobs, right out of school,  $4-5 more than what the 
> agencies will offer you just starting out as a traveler, regardless of 
> previous experience.  Also, travel = NO BENEFITS.  Some of the agencies 
> pretend to have benefits, but as I said before, it's near impossible to work 
> with enough continuity to remain covered.
>  Also, big warning:  A lot of the "agencies" aren't really agencies at 
> all, but someone with an 800 number and a website and some hold music.
>   It ain't all speedboats and supermodels, if that's what you had in mind.
>
>   Sincerely,
>
>   Jay A. Lundgren, M.S., HTL (ASCP)
>
>
> On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen  wrote:
>
> > Thanks, this is very timely as I'm looking into temp/travel HT.
> > Any suggestions where to look, which agencies?
> >  Thanks, Jeff Lowen  HT/MLT(ASCP)
> >
> > > Date: Mon, 9 Sep 2013 17:52:34 -0500
> > > From: jaylundg...@gmail.com
> > > To: lpw...@sbcglobal.net
> > > Subject: Re: [Histonet] Travel Histology Technician Jobs
> > > CC: histonet@lists.utsouthwestern.edu; cjac...@clinpath.com
> >
> > >
> > > I've been traveling for the past 16 years! Not everyone who wanders
> > > is lost.
> > >
> > > Sincerely,
> > >
> > > Jay A. Lundgren,
> > > M.S., HTL (ASCP)
> > >
> > >
> > >
> > >
> > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk
> > > > >wrote:
> > >
> > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in
> > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a
> > > > workshop on "Work and Play Across the USA - A Guide to Being a 
> > > > Traveling Tech".
> > > > http://www.histoconvention.**org/
> > > > 
> >
> > > > Click on Schedule
> > > >
> > > > Peggy A. Wenk, HTL(ASCP)SLS
> > > >
> > > > -Original Message- From: Christopher Jacobs
> > > > Sent: Monday, September 09, 2013 1:30 PM
> > > > To: 'histonet@lists.**utsouthwestern.edu<
> > histonet@lists.utsouthwestern.edu>
> >
> > > > '
> > > > Subject: [Histonet] Travel

RE: [Histonet] Embedding

2012-05-25 Thread Norton, Sally
We make sure to clean the wells also.  Little flecks of tissue are almost 
always in there after embedding.

Sally Norton
Seattle Children's

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, May 25, 2012 10:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding


We are having a problem with floaters in our blocks which occur during 
embedding.  We have multiple forceps which are placed in heated wells and each 
cassette is embedded with a new forcep.  We also wipe with a gauze, but we are 
still getting floaters embedded in the cassette from time to time.

Does anyone do anything else to prevent this?
Thank you, Ann
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RE: [Histonet] (no subject)

2011-10-17 Thread Norton, Sally
Hi Miha, we do many muscle bxs here as well, & the information Becky has sent 
you is exactly how we do it.  This should help you!

Sally Norton
Seattle Children's Hospital 
Seattle, WA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky
Sent: Monday, October 17, 2011 1:41 PM
To: 'Miha Tesar'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

I am forwarding this response from a fellow worker, D Kaylor
(The techs here have consistently good results when freezing fresh muscles
for enzyme histochemistry.

The small holes are freeze artifact.  Usually they occur when the muscle is not 
frozen fast enough.  We use methyl butane floating in a liquid nitrogen bath.  
Either way, the temp is critical.  It needs to be at least -155 degrees C.   
The muscle must be submerged and held under for about 45 seconds to one minute. 
 Do not start or dip the muscle.  Once started it must be held submerged.  We 
have never had any issues with humidity.   Are the muscle bx swimming in 
saline?   We have better results when they are submitted on saline moistened 
gauze.
We use positively charged slides to pick up our sections.  We have some 
wrinkles but that is due to the sectioning of frozen tissue not the type of 
slide.


Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, October 15, 2011 4:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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[Histonet] Jones stain boo boo

2011-05-23 Thread Norton, Sally
I accidentally put my renal slides in the Methanimine Silver  (after
Periodic Acid) before putting them in the Thiosemicarbizine.   Slides
take on a yellow hue which makes the H&E counterstain muddy.  Is there a
way to correct this?

 

Thank you,  

 

Sally Norton

Histotech

 



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RE: [Histonet] LabVision line of instrumentation, antibodies and reagents

2009-03-24 Thread Norton, Sally
Hello,

We do IF's on renal biopsies and skin.  We were getting our antibodies
and Protein Block from ThermoShandon which is now Lab vision.  We are
now not getting our supplies when needed.  We have Protein Block on
order and were told we would not get any until end of April, which was
then amended to the end of March.  In the meantime we've run out and
can't do a renal biopsy until we get some.  Does anyone out there do IF
on Renal's and who is your supplier.  We are looking to find someone
new.

Thank you,

Sally Norton, HT
Seattle children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
rg...@bmnet.com
Sent: Tuesday, March 24, 2009 12:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LabVision line of instrumentation,antibodies and
reagents

I was wondering if there are any clinical labs that use the LabVision
line
of instrumentation, antibodies and reagents.
We are a small hospital that does about 30 predilute antibodies and are
considering changing from our current vendor/instrument.

I would appreciate good, & bad experiences.  I don't want to go into any
change without knowing.

Thanks,

Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax


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RE: [Histonet] Re: Log Book

2009-03-03 Thread Norton, Sally
Kathy

We use the same method as Michelle described.  We mark next to the patient name 
if there is more that one specimen.   We do not take the specimen if there is 
any discrepancy.  The OR has to fix it first and sign a form taking 
responsibility for the specimen.

Sally
Seattle Children's Hospital


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shelly Coker
Sent: Tuesday, March 03, 2009 4:22 PM
To: histonet@lists.utsouthwestern.edu; gorh...@verizon.net
Subject: [Histonet] Re: Log Book

Kathy,
 
I worked in a hospital at one time where we were expected to pick up the 
specimens from the OR.  We actually created a log book that stayed in the OR.  
The nurses simply placed a patient label in a spiral notebook and noted the 
specimens beside it.  When we went to pick them up, we checked each specimen 
off and put our initials, date and time in the book. We would not pick up any 
specimens with a discrepancy until the nursing staff corrected it.  In the 
instance you are describing, the specimens for said patient would not have been 
picked up until the nursing staff corrected the log book or found the 
specimen.  We did also date/time stamp the requisitions when we got back to the 
lab as well.
 
Good luck and I hope you find your specimen :(   
 
Michelle

Good Monday Morning,  We had a serious incident Friday with O.R.  My aide went
down to get the specimens from O.R. about 9am. (which were left overs from the
night before).  She did not stamp in the specimens before she left.  When I had
time to stamp them in and record them in the log book I discovered that the
colon was not there.  Two other specimens from that patient where in the bag but
no colon.  So I went down to O.R. to see where it was.  Of course no one knows
what happened to the colon.  The doctors are furious by all means.  Now the O.R.
thinks the path lab screwed up.  So my questions is how do others log in the
specimens as they come into the lab.  We have 2 couriers that brings specimens
when we are not in the lab from other hospitals.  How do you make sure that whom
ever brings the specimens actually brings the ones they say they do?  Do you
have a log book that every specimen that is brought into the lab is written down
by the person who brings it in?  Right now we have a log book but it is written
in as we are accessing  the specimens.  So the specimens may have been there
overnight. We are a very small lab and we do almost everything by hand including
writing in the log book.  Someday we want to be able to scan by bar codes but
right now we can not do that. Thanks for any help you can give me. 
Kathy Gorham, H.T


  
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Children's Hospital and Regional Medical Center is now Seattle Children's.
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[Histonet] Alcoholic Saffron

2008-11-25 Thread Norton, Sally
Hello Histonetters,

 

Does anyone use the Alcoholic Saffron Solution pre-made from Rowley
Biochemical Institute?  We would like to know how the long the
expiration period is for it.  We either tossed or didn't receive an MSDS
sheet on it.

 

Thank you,

 

Sally Norton, HT

Seattle Childrens Hospital

Seattle, Wa

Children's Hospital and Regional Medical Center is now Seattle Children's.
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RE: [Histonet] Be careful, be very careful when you Vote!

2008-11-04 Thread Norton, Sally
Think that the Histonetters site is the wrong place to be giving your
political views.  

Sally Norton, Seattle, Wa

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Sam
Histology
Sent: Tuesday, November 04, 2008 6:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Be careful, be very careful when you Vote!

Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein
Obama
Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann
Dunham
of Wichita, Kansas. (white atheist ).

When Obama was two years old, his parents divorced and his father
returned
to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to
Jakarta
with Obama when he was six years old. Within six months he had learned
to
speak the Indonesian language. Obama spent "two years in a Muslim
school,
then two more in a Catholic school" in Jakarta. Obama takes great care
to
conceal the fact that he is a Muslim while admitting that he was once a
Muslim, mitigating that damning information by saying that, for two
years,
he also attended a Catholic school.

Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who
migrated
from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a
white
atheist from Wichita, Kansas-at the University of Hawaii at Manoa.
Obama,
Sr. and Dunham divorced when Barack, Jr. was two.

Obama's spinmeisters are now attempting to make it appear that Obama's
introduction to Islam came from his father and that influence was
temporary
at best.

In reality, the senior Obama returned to Kenya immediately following the
divorce and never again had any direct influence over his son's
education.

Dunham married another Muslim, Lolo Soetoro who educated his stepson as
a
good Muslim by enrolling him in one of Jakarta's Wahabbi schools.
Wahabbism
is the radical teaching that created the Muslim terrorists who are now
waging Jihad on the industrialized world.

Since it is politically expedient to be a Christian when you are seeking
political office in the United States, Obama joined the United Church of
Christ to help purge any notion that he is still a Muslim.
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