Re: [Histonet] Antibody dilution help
Charles, The easiet way to remember it is this: you want a 1:100 dilution - so you want 1 part antibody in 100 parts of diluent. I was taught that meant add 1 part antibody to 100 parts of diluent. However, there are some who add 1 part anitbody to 99 parts diluent.Thus you end up with 1 part antibody in 100 parts total. Some folks read 1:100 as 1 part in 100, some folks read it as add 1 part to 99 to make 100 parts. confused yet? I found this Let's see what others have to say. Sincerely, Paula Sicurello On Friday, August 30, 2024 at 07:57:36 AM PDT, Charles Riley via Histonet wrote: Hello everyone, Not sure if it's just burnout or Covid brain but I am having issues with remembering the right way to dilute my antibodies, and all the calculators online seem to give different responses. I have 100 microliters of 1mg/mL antibody and I want to make a 1:100 dilution. How much diluent do I add to do this? And if anyone has any calculators that they like using that would be awesome too ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histo haiku
Pink and purple stainThe slide pink and not too blue.Benign, very good news Sincerely, Paula Sicurello On Sat, Jul 20, 2024 at 1:29 PM, Bob Richmond via Histonet wrote: Saffron, eosin, Stamens of autumn crocus: So yellow! So red! This actually is a haiku, with the obligatory seasonal reference (kigo) and the break (English punctuation equivalent of Japanese kireji, "cut-word"). Whether it adequately expresses wabi-sabi is a question this Episcopalian will leave to the Buddhists among us. Bob Richmond Retired samurai pathologist Maryville, Tennessee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] De-mummification procedure?
Jay, Groaning and eye rolling. Sincerely, Paula Sicurello On Wednesday, May 15, 2024 at 12:06:24 PM PDT, Jay Lundgren via Histonet wrote: The Mummy's major weakness is fire, a common weakness among the undead. Since mummies tend to be dry and coated with various oils and resins, the revenant tends to burn very well. Thus fire is the only way to destroy the Mummy forever (aka de-mummification). Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, May 14, 2024 at 4:21 PM Cheryl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi and help? A tech on another forum is dealing with a catastrophe. > > Does anyone have the glycerin de-mummification procedure? Haven’t used it > in decades but with what those samples went through it may be their last > chance as any sort of readable slides. > > TIA!! > > Cheryl > > > Please excuse typos-sent from a phone. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXT] Reagent alcohols and tissue processors
Hi Kara, I do like the VIPs. Besides getting rid of the NBF, the alcohols are getting rid of the water as well. I learned EM first, so my processing thoughts are probably biased by that. Paula Yahoo Mail: Search, Organize, Conquer On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 p: 918-281-9060 w:www.unthsc.edu/corelabs From: Paula Sicurello via Histonet Sent: Thursday, March 28, 2024 8:04 AM To: HistoNet Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70% but I favor a lower %. One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent alcohols and tissue processors
Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70% but I favor a lower %. One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A Question for DOD Histology Labs
Good Morning and Happy Monday, If you work at a Department of Defense (DOD) Histology lab, can you tell me what make and model of cassette printer and slide writer you use? Do they have an ATO? We are in the market for both and want to know what y'all are using. Plus how do you like whatever it is you are using? Thank you, Paula Yahoo Mail: Search, Organize, Conquer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue cassettes
Why use a different wax for embedding? Cost. Those who don't fully understand the art & science of histology think a wax is a wax is a wax. Bon-bon, Paula Sent from Yahoo Mail on Android On Sat, Feb 10, 2024 at 11:23 AM, Carl Hobbs via Histonet wrote: Why would anyone use a different wax for infiltrating and embedding? Yeskeep them specimens molten until embedded Thanks for your input, Paula Time flies like an arrow Fruit flies like a banana BonBon-illy Carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin pH
Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Logging everything you do.
I'm glad to hear that the staff suggested the tracking of tasks. If I had to guess the items causing the most trouble are: Poorly grossed specimens if someone other than a Histotech grosses - all of which cause poor processing: too big filling the entire cassette; too thick squishing out the top and bottom causing waffling; not decalled long enough needing extra long surface decal to cut. Microtomes: messed up knife holders with all sorts of dings and dents so the blade doesn't clamp right causing thick & thin sections or chatter; block clamping head getting out of alignment or full of wax resulting in poor sections. Cruddy blades - just because it costs less doesn't make it a better blade; knife marks from hitting staples that the grosser swore they took out. Waterbath: too cold and getting wrinkles; too hot and sections poof into non-existance. Floaters from not cleaning the surface between blocks; floaters from someone's skin cells because they don't like wearing gloves when sectioning. Supplies: or lack of, it seems like there may still be supply chain issues. Everyone's favorite: clinicians calling asking why it's taking so long to get results. This week I had one tell me she got her flow results in a day and Histo has had the specimens for two days - so what's the hold up? Please share what items are troubling after the lists are compiled. It will be interesting to see what they have to say. Sincerely, Paula Sicurello On Sat, Jul 22, 2023 at 1:39 PM, Samantha Golden via Histonet wrote: Thanks for taking the time to respond. It was actually staff who suggested we see what tasks are being performed, when, and how long. No punitive action is being taken. As I indicated, we, as in the team, want to find the pain points. I do continually ask for ideas and feedback; staff repeatedly tell me they cannot see any other way to do things. I am on the bench daily and I know what the problems are. But like you said, I want staff to feel involved in the discussion, and this was their idea. Sent from my iPhone > On Jul 22, 2023, at 4:15 PM, Terri Braud via Histonet > wrote: > > As someone who has been a supervisor in 3 institutions for 35 years, this is > not that way to improve productivity. To log in every minute of activity > feels very punitive and I can't imagine that it would be well received by > staff. The best way to improve productivity is to start by asking each tech > on a daily basis what problems do they feel impacts productivity. Some items > already have general standard established such as embedding, cutting, > staining. Ask your techs for their ideas. I'm sure they will have some > valid ones. Don't let the talks dissolve into complaining. Ask for concrete > ideas for improvement that can be tried. Look for duplicity in work, records, > and labeling. Make sure they have the right tools to do their job. Get on > the bench and see it for yourself. There is nothing like first had > experience to find the weak spots. > They worst way to improve productivity is to require such an onerous demand > such as a task log. All you are doing is slowing productivity, not > improving. > Respectfully, Terri > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-2021 > Honesty > AccouNtability > AgiLity > CoLlaboration > CoMpassion > > > Message: 5 > Date: Fri, 21 Jul 2023 21:42:20 -0400 > From: Samantha Golden > Subject: [Histonet] Productivity log > I have asked staff to start logging all the tasks they perform and the amount > of time it is taking them to complete. We would like to identify pain points > and waste in an effort to improve our overall productivity. Rather than > reinventing the wheel, does someone have a form they?ve used in the past that > they would be willing to share? > Thank you for sharing your experience. > Samantha > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Logging everything you do.
Is this documentation for a work/labor study to determine pricing or show upper leadership how hands-on and time consuming histology is? If not, then what you are asking is punitive. Not to mention that it will kill any comradery that your histotechs may have. This will be seen as either a competition or a way to point out those that are not as fast/skilled/etc. I can hear it now (where I used to work had the histotechs write stuff down for all to see (I stopped that practice right away) "Betty is picking and choosing her blocks" "Bob is leaving all the bone blocks for me to do" "What are YOU going to do about Joe being so slow?" "Why am I the only one who cuts the prostate biopsies?" That's only a few of the nicer complaints I heard. Plus it will be a quick way to make for an over all negative environment. I agree with Terri - ask them for to improve, streamline, etc. Asking the staff for help to improve the lab goes a long way to making a good working environment. With fewer and fewer people becoming histotechs - the work environment is crucial. Sincerely, Paula Sicurello On Saturday, July 22, 2023 at 01:02:21 PM PDT, Terri Braud via Histonet wrote: As someone who has been a supervisor in 3 institutions for 35 years, this is not that way to improve productivity. To log in every minute of activity feels very punitive and I can't imagine that it would be well received by staff. The best way to improve productivity is to start by asking each tech on a daily basis what problems do they feel impacts productivity. Some items already have general standard established such as embedding, cutting, staining. Ask your techs for their ideas. I'm sure they will have some valid ones. Don't let the talks dissolve into complaining. Ask for concrete ideas for improvement that can be tried. Look for duplicity in work, records, and labeling. Make sure they have the right tools to do their job. Get on the bench and see it for yourself. There is nothing like first had experience to find the weak spots. They worst way to improve productivity is to require such an onerous demand such as a task log. All you are doing is slowing productivity, not improving. Respectfully, Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion Message: 5 Date: Fri, 21 Jul 2023 21:42:20 -0400 From: Samantha Golden Subject: [Histonet] Productivity log I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? Thank you for sharing your experience. Samantha ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QIHC
Well..I did not pass the exam on my second attempt. Waa! Anyway, it got me wondering: are there statistics out there that give a break down of the pass rate of the QIHC exam? What's the percentage of HTLs that are QIHC? Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sections sticking to tweezers
Hi Ken, A few questions: 1. What is the melting point of the paraffin you use? 2. Is there a specific reason the temperature of your water bath is 38.5 degrees? 3. Do your forceps have any type of imperfections? Bumpy, or ridged? Something that could snag on the sections? If there isn't a specific reason for the water bath temperature to be that low (cutting brain sections), I'm thinking that is playing a large part in your sticky sections. It is common to have the water bath 5 - 10 degrees below the melting point of the wax. It helps the sections stretch out from the compression that happens when being cut. Let's see what others have to say about your sticky situation. Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote:Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote: Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cutting with glassknifes
Gurtrude, It's not a common practice anymore to use glass with a microtome. If you are using a methacrylate you have to use glass, a razor blade isn't strong enough to handle the shear forces. The art of hand breaking the kniveswell that's becoming a lost art. I can't do that anymore. I have to use a knifebreaker. Paula Sicurello On Sun, Apr 30, 2023 at 9:40 AM, Ross Langston via Histonet wrote: Hello- I do not work in a conventional histology lab, but we exclusively use resin (JB-4) Embedding and glass knives on MT-1s to do histology on gonadal tissue for fisheries research. Although the MT-1 is an ultramicrotome, we use it more like a conventional microtome. The reason we do this rather than wax histology is that our "lab" travels to some pretty remote areas (Papua New Guinea, Micronesia, etc) and you need less stuff to do resin histology (really only a JB-4 Kit, microtome, and some glass knives plus whichever staining method you prefer) and can even do it without power. We actually have a workshop coming up soon in Guam, if you are interested. Ross On Sun, Apr 30, 2023 at 6:19 AM Gudrun Lang via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Dear histonetters! > > I have a question for the experienced histo-people. > > Is it still in practice to use glassknifes for cutting on rotary- or > sliding-microtomes? For plastic-embedded tissue in light-microscopy? > > Or ar glassknifes only found in EM-technique? > > > > I am just curious, because I assume, it is a rather difficult skill to > handle. > > I made a short internet-search, but got no hints on this issue. > > > > Thank you and kind regards > > Gudrun Lang > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor Schedule Validations
The one thing that bothers me about the preloaded programs is the paraffin temperature is set at 65 degrees C. That's close to 10 degrees higher than the melting point of our paraffin. Does anyone know why the temp. is so high? To me, a temp that high will cause problems with IHC and small biopsies. Has anyone changed it? Or do folks go along with it just because it's already there? I inherited that temp, to change it now would be pain since it is the one we use the most. If I validated our current Peloris 3, I would have set the wax temperatures at 60 degrees. Sincerely, Paula Sicurello On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet wrote: Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted. I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mice/Rat brain
Hi Renee, Brains are notorious for being difficult. Processing has to be just so, embedding has to be just so, and sectioning? Well, just plain tricky. It sounds like your water bath may be a smidge too cool. Brains are picky that way. Water bath too warm? Poof! The section explodes. Too cool? Wrinkles. Can you use vibratome sections or do you need FFPE sections? Paula Sicurello On Thu, Mar 9, 2023 at 6:51 AM, Renee Fisher via Histonet wrote: We are having issues with mice and rat brain sections. There are folds in the tissue. We just can’t seem to get a flat section without folds. Any suggestions? Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.
Ted Pella, Inc. (Support small business) they sell EM, and Histology items. Paula Sicurello On Thu, Mar 2, 2023 at 5:19 AM, Karen Heckford CA-San Francisco via Histonet wrote: I got mine through Thermo Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Wed, Mar 1, 2023 at 3:04 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu> wrote: > USE CAUTION - EXTERNAL EMAIL > > .. > Who sells that block deparaffinizer doohickey that everyone has in their > lab. What brand is it? Leica? I don't have one in front of me right now. > > Thanks, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > Histotechs are the foot soldiers in the battle against Cancer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GHohhm5t61fx5WIqk10A1j1agEowfqUHV4ta3OZ5US9GoWlK5ZYYYzLLhsql0_vxxCDE4T0OtBxGKbplHxK4mah8O7khxSPV-w$ > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette printers
Good Morning Listers, We are in the market for a new cassette printer. Has anyone used the General Data LaserTrack FLEX system? I'm interested in how well it actually prints on 3 sides of the cassettes. Any input about any other vendors printers is helpful. I have quotes from General Data, Sakura, and Leica. Please provide your opinions and related experiences with each of the vendor's cassette printer. We currently have a General Data CL12 that is dying a slow, painful death. Thanks oodles for your help. Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Epon Resin Embedding Question
Hi Brian,Unfortunately, no. The most you can do is to put it back in the oven and let it bake forva very long time. You won't be able to section it. It will gum up the knife edge. If they are using a diamond knife they need to clean it right away. To add insult to injury, the poorly polymerized blocks are a safety hazard. Epoxy resins are all toxic, carcinogenic, mutagenic, a reproductive hazard, or all of the above combined. It’s only when the resin is fully polymerized that it becomes an inert plastic. Perhaps someone else out in Histoland (Tim Morkin, are you out there?) has had luck dealing with blocks like those. Sincerely, Paula Sicurello On Wed, Sep 28, 2022 at 12:27 PM, Cooper, Brian via Histonet wrote: Good afternoon Histonet, Asking this question for a friend: Is there a way to correct an improperly prepared Epon resin embedded block? Following embedding, the block is soft and "tacky" to the touch. This block is proving VERY difficult to section. Any assistance from those of you doing EM in your labs would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QIHC certification
Hello Listers, Happy Saturday, it's a beautiful day here in San Diego. I have a question: Is there an alternative route for the experience requirement for the QIHC? I need to get certified but I haven't been doing IHC at my new job. I have been doing IHC since the early 1990's (remember the VectaStain ABC kits?). At my prior gig I did manual IF on skin, kidneys (direct), and heart biopsies (indirect). I don't have enough time to work the required amount at my new job. I can't get a letter from my previous supervisor (she left for greener pastures). Suggestions anybody? Thanks oodles. Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part Time Histologist Needed In San Diego, California
Hello Listers, UC San Diego Health has an opening for a part-time Histologist. HT/HTL required. Experience in a high volume academic setting preferred. We need someone with strong sectioning and trouble shooting skills. Familiarity with IHC or Electron Microscopy a plus. Go to jobs.ucsd.edu and look for job #84828 Sincerely, Paula PaulaSicurello, HTL (ASCP)CM InterimSupervisor: EM, Histology, IHC UCSan Diego Health 200Arbor Drive SanDiego, CA 92103 (P):619-543-2872 psicure...@ucsd.edu Confidentiality Notice: The information transmitted in this e-mailis intended only for the person or entity to which it is addressed and maycontain confidential and/or privileged material. Any review,retransmission, dissemination or other use of or taking of any action inreliance upon this information by persons or entities other than the intendedrecipient is prohibited. If you received this e-mail in error, pleasecontact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] UC San Diego Anatomic Pathology Histology Labs is Hiring
Hello, We are hiring a histotechnologist for our growing histology lab. UCSD is adding a new hospital and soon our volume will increase. We are looking for an experienced histotech. Please go to: https://jobs.ucsd.edu/bulletin/job.aspx?cat=health&sortby=post&jobnum_in=81586. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Start up Vet/Animal histology laboratory
Hi Linda, I've set up three histology labs for animal research: one in an academic setting, one for a pharmaceutical firm, and one for a research foundation. 1. Having money to buy the proper equipment is tricky, start with used equipment and make due until you can afford to buy new. As long as you buy the equipment from somewhere that either provides a warranty or the equipment is so inexpensive that you don't care if it breaks (as long as it gets you going). 2. Having the investors (or whom ever is supplying the majority of the funding) understand that often times histology labs that are providing a service to researchers do not make money. In the early stages they are black pits you pour money into. 3. Building up a clientele requires a lot of advertising and offering low prices (undercutting the competition even if it means you suffer a loss). 4. Have a method of billing, invoicing, and collecting on those invoices can be a challenge. Simple bookkeeping software like Quickbooks or FileMaker will help you considerably. 5. Write your SOPs as soon as you can. Having the methodology of every technique, stain and procedure you will be doing is invaluable. When you get around to be able to hire staff these will be a lifesaver. Having established SOPs allow you to make sure your staff also follow the same procedure. Reproducability and consistency is a must when establishing a histology lab. There is no shame in borrowing heavily from SOPs you have come across in the past. Rely on colleagues to provide SOPs and pointers. 6. Make sure that the place you select for the business allows for proper ventilation of all the reagents and chemicals. You want to limit your exposure to the chemicals in the lab to the maximum point possible. Safety should be of the utmost importance in the new lab. Those are just a few things I can think of. Most important is to enjoy the ride-it's going to be hard and sometimes frustrating work-but building the business is part of the fun. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health On Saturday, July 9, 2016 5:08 PM, Linda via Histonet wrote: Hello Histoland, I am inquiring if anyone has started their own lab to do histology services for veterinarians or animal research tissues? Or anyone who has started a private lab? What are your experiences? What didn't you expect? Thank you in advance. Regards, Linda Dee, BGS,HT(ASCP) lmd...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP 6 owners manual
Hello Netters, Does anyone have an electronic copy of the owners manual for a Tissue Tek VIP6? If you do can you send it to me? Sakura charges $129 for one!! Thanks, Paula UC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 10% NBF Question
Hello Listers, Has anyone had any problems with fixation using Richard-Allan Scientific 10% NBF? Some of our gastric biopsies are looking a little off. The rest of the biopsies fixed in the same NBF and processed on the same processor look fine. This issue would have come up in the last couple of weeks. I know that Richard-Allan recalled their NBF a couple of years ago because the % was 0-3 not 10%. Please let me know if any of you are experiencing something similar. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Results of My Histology Survey-Finally!
Well Listers, I finally get around to posting my survey. Only 35 people took it but it still provided useful information and I got an A in my class. Thank You to the 35 who participated All the data: 23 HT's, 8 HTL's, 4 with other certification (2 with HT/HTL) took my survey 24 years of experience average. Range 5 to 45 years. 49 blocks per hour, average cutting. Range 19 to 120 69 slides per hour, average. Range 35 to 160/hour 7 average number of staff. Range 1 to 22 278 blocks per day per lab, average. Range 10 to 1000 467 H&E slides per day, per lab, average. Range 10 to 1500 92 IHC slides per day, per lab, average Range 2 to 300 19 special stain slides per day, per lab, average Range 1 to 100 My take away from this was that we are getting older, we are working faster with fewer staff and we use automation to our advantage. I think none of this is surprising to you. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Traveling Histologists?
Good Morning Listers, Are there companies out there that work with traveling histologists? If so, can you send me their contact information? Companies dealing with traveling histologists are free to contact me as well. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Workload and Productivity Survey closing this week
Good Morning Listers, I will be closing my survey this week on Friday. If anyone else would like to take it, please follow this link: Histology Questionaire Survey | | | | | | | | | Histology Questionaire SurveyWeb survey powered by SurveyMonkey.com. Create your own online survey now with SurveyMonkey's expert certified FREE templates. | | | | View on www.surveymonkey.com | Preview by Yahoo | | | | | Thank you for helping me with my project. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Questions part 2
Good Evening Netters, I've created a short survey using Survey Monkey. Please take my survey and help me gather information I can use in my class and to help update the productivity standards and staffing benchmarks for our Histology labs. https://www.surveymonkey.com/r/8HFSY3V Thank you and thanks to those who took my first survey. The results look interesting. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Lab Survey Questions
Hello Netters, Thank you for taking my survey. I will use your replies for my research project and I will also let everyone on the list know the results as well. From what I've found, the surveys performed in the past asked similar questions but more at the institution level. I would like to get information at the individual level. There are 16 questions. Please reply to this email offline with your answers. Thank you for helping me. Let's hope I get an A! :-) Here we go:Questions aboutyou: 1. Education (highest achieved): a. High school b. Associates degree c. Bachelor’s degree d. Master’s degree e. PhD or MD 2. Certification: a. None b. HT c. HTL d. Other, please describe 3. How did you learn histology? a. On the job b. Histotechnology school 4. How many years of experience in histology? 5. How many blocks can you embed in an hour? 6. How many of the following can you produce in an 8 hourday? (You can calculate from howmany of each task you can perform in one hour: 20 blocks with one slide each per hour = 160 blocks and 160 slides) a. Blocks b. Slides Questions aboutyour place of work: 7. How many blocks does your lab embed a day? 8. How many staff does your lab have? a. Technical (HT, HTL, etc.) b. Non-technical (lab assistants, etc.) 9. How is IHC handled in your lab? a. Separate staff (if so how many?) b. All staff perform 10. Howmany IHC slides does your lab stain per day? 11. Howmany of the following can your lab run? a. Antibodies (total number available for testing) b. Special Stains (total number available for testing) 12. Doesyour lab use automation? Yes or No Ifyes, please describe: 13. Whoprepares the slides prior to release to the pathologist? 14. Whofiles the blocks and slides? 15. Whattype of institution do you work at? a. Hospital based Histology lab b. Clinic based Histology lab c. Independent lab (such as a referral lab) 16. BonusQuestion: Has your family stopped askingwhy you come home with blue or pink-orange fingers? OPTIONAL: Please add your comments, suggestions,or thoughts. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Related Questions?
Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions. I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in a reply email? I hope to keep it between 15-20 questions. I even promise to share my results once I compile all the information. Suggested questions are also gratefully accepted. Toodles! Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Related Questions?
Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions. I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in a reply email? I hope to keep it between 15-20 questions. I even promise to share my results once I compile all the information. Suggested questions are also gratefully accepted. Toodles! Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Test email
Good Morning Netters, This is a test to see if I can get an email through using this email account. My posts haven't been making it through and I know you miss me. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Source for ultra low temperature immersion thermometer
Good Morning Histolanders, Does anyone have a source for an immersion thermometer that can be used to measure the temperature of the isopentane or 2-methylbutane or methylbutane ( you choose) ;-) used for freezing muscle biopsies? I have searched using Dr. Google and cannot find one that goes below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP Question
Good Morning Netters, How many antibodies are y'all testing to meet the CAP guideline (COM.04250) that requires comparison testing of equipment performing the same test (autostainers, for instance) twice a year? I read it as requiring all antibodies are tested on all platforms, but that seems a bit much. Enquiring minds want to know. Thanks a bunch! Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H&E Stainer Question
Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Per Diem Positions at UC San Diego Health System
Good Afternoon Listers, UCSD Medical Center Department of Anatomic Pathology has two per diem positions open in Histology. Histology experience is a must. If you are interested email your resumes to: Craig Fisher at *cfis...@ucsd.edu * and Mike Mathhew at *mimatt...@ucsd.edu * Thank you and have a nice day. Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A question about a new CAP requirement
Hello Netters, How are you all interpreting this new requirement (COM.4) with regards to multiple autostainers for IHC? Thank you in advance, Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] COM.40000 (new)
Hello Netters, How are you all interpreting this new requirement with regards to multiple autostainers for IHC? Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BS in Histotechnology
I tried to teach the starving graduate students the same things, with similar results :-) And it's not even Friday. Paula On Tue, Mar 24, 2015 at 5:05 PM, William J. O'Connor III wrote: > I have monkeys where I work, cynos. We tried to train them to not bite > our fingers off - I wouldn't even try to teach them to use a microtome. > > > -Original Message- > From: Michael Ann Jones > To: Stedman, Nancy ; Mark Turner > ; Paula Sicurello > Cc: histonet ; Timothy Morken < > timothy.mor...@ucsf.edu>; Jennifer MacDonald ; > Marcum, Pamela A > Sent: Tue, Mar 24, 2015 4:30 pm > Subject: Re: [Histonet] BS in Histotechnology > > Thanks Dr. Stedman! Good to hear! > Michael Ann > > > > > On 3/24/15, 3:22 PM, > "Stedman, Nancy" > wrote: > > >As a pathologist > I'd like to apologize for all the pathologists who have > >made comments like > this.. forget trained monkeys and dogs, most (all?) > >pathologists cannot cut > slides either, at least not slides they'd want to > >try to read. I know I > can't. > > > >-Nancy Stedman > > > > > > > > > >-Original Message- > >From:histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] > On Behalf Of Mark > >Turner > >Sent: Tuesday, March 24, 2015 4:26 PM > >To: Paula > Sicurello; Michael Ann Jones > >Cc: histonet@lists.utsouthwestern.edu; Timothy > Morken; Jennifer > >MacDonald; Marcum, Pamela A > >Subject: RE: [Histonet] BS in > Histotechnology > > > >I once worked with a Pathologist who said she was in a > group meeting of > >other pathologists when one of them blurted out that a > trained monkey > >could cut slides. My pathologist, having had the opportunity > to review > >some cases from the offender's laboratory, promptly replied "Yes, > and > >with the quality of your slides it looks like you did just that." > She > >shut down the other pathologist really quickly, and as far as I know, > we > >never received another case to review from him. My pathologist was > not > >about to let that kind of arrogance stand. She was one of the > best > >bosses I ever had! > > > >Mark Turner, Ph.D., HT(ASCP)QIHC > >Manager, > Histology/IHC > > > > > >-Original Message- > >From:histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] > On Behalf Of Paula > >Sicurello > >Sent: Tuesday, March 24, 2015 3:47 PM > >To: > Michael Ann Jones > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; > Marcum, Pamela > >A; Timothy Morken > >Subject: Re: [Histonet] BS in > Histotechnology > > > >I've had more than one pathologist tell me a monkey could > do my job. > >Though one of them said it with a smile and added "a very highly > skilled > >and well trained monkey", he was one of the few who knew > better. > > > >How many of us monkeys have trained the whining and complaining > residents > >how to do things correctly? > > > >Paula > > > >On Tue, Mar 24, 2015 at > 12:29 PM, Michael Ann Jones > >wrote: > > > >> OMG Pam~ our > pathologist said the exact same thing to us when we > >> started our Grossing > training. > >> Sheesh. . . > >> Michael Ann > >> > >> > >> > >> > >> On 3/24/15, 11:52 > AM, "Marcum, Pamela A" wrote: > >> > >> >That was nicer than > the pathologist who told me years ago, "any > >> >monkey could be trained to do > my job". I basically did not take the > >> >job I was interviewing for at the > time. At least the next interview > >> >went a lot better and I did take the > job. > >> > > >> >Pam > >> > > >> >-Original Message- > >> >From:histonet-boun...@lists.utsouthwestern.edu > >> > >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > >> >Sanders, > Jeanine (CDC/OID/NCEZID) > >> >Sent: Tuesday, March 24, 2015 12:30 PM > >> >To: > Sue; Timothy Morken > >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer > MacDonald > >> >Subject: RE: [Histonet] BS in Histotechnology > >> > > >> >I agree, > BUT>>>>>>>>>>>>>as long as many pathologists think you can > &
Re: [Histonet] BS in Histotechnology
It is an art! That's what I try to get across to those that I am teaching an mentoring. Think of every slide as an example of your skill and artistry. Also to think of every photo you take in EM as something that will be published. Besides, I like pretty colors much more than a blue line on a gel. :-) Paula On Tue, Mar 24, 2015 at 3:33 PM, Garreyf wrote: > I am trying to find (hire) a histotech and will make sure I don't use the > words "trained monkey" in my interviews. . Ha ha. > I truly value and appreciate a skilled and motivated histotech. I've had > to train myself to cut my own sections in order to understand the process > better. It is a great field; it's like art to me.There is no room for > monkeys in my book. > Garrey > > Sent from my iPhone > > > On Mar 24, 2015, at 5:22 PM, Stedman, Nancy < > nancy.sted...@buschgardens.com> wrote: > > > > As a pathologist I'd like to apologize for all the pathologists who have > made comments like this.. forget trained monkeys and dogs, most (all?) > pathologists cannot cut slides either, at least not slides they'd want to > try to read. I know I can't. > > > > -Nancy Stedman > > > > > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner > > Sent: Tuesday, March 24, 2015 4:26 PM > > To: Paula Sicurello; Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer > MacDonald; Marcum, Pamela A > > Subject: RE: [Histonet] BS in Histotechnology > > > > I once worked with a Pathologist who said she was in a group meeting of > other pathologists when one of them blurted out that a trained monkey could > cut slides. My pathologist, having had the opportunity to review some > cases from the offender's laboratory, promptly replied "Yes, and with the > quality of your slides it looks like you did just that." She shut down the > other pathologist really quickly, and as far as I know, we never received > another case to review from him. My pathologist was not about to let that > kind of arrogance stand. She was one of the best bosses I ever had! > > > > Mark Turner, Ph.D., HT(ASCP)QIHC > > Manager, Histology/IHC > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > > Sent: Tuesday, March 24, 2015 3:47 PM > > To: Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, > Pamela A; Timothy Morken > > Subject: Re: [Histonet] BS in Histotechnology > > > > I've had more than one pathologist tell me a monkey could do my job. > > Though one of them said it with a smile and added "a very highly skilled > and well trained monkey", he was one of the few who knew better. > > > > How many of us monkeys have trained the whining and complaining > residents how to do things correctly? > > > > Paula > > > > On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones < > mjo...@metropath.com> > > wrote: > > > >> OMG Pam~ our pathologist said the exact same thing to us when we > >> started our Grossing training. > >> Sheesh. . . > >> Michael Ann > >> > >> > >> > >> > >>> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > >>> > >>> That was nicer than the pathologist who told me years ago, "any > >>> monkey could be trained to do my job". I basically did not take the > >>> job I was interviewing for at the time. At least the next interview > >>> went a lot better and I did take the job. > >>> > >>> Pam > >>> > >>> -Original Message- > >>> From: histonet-boun...@lists.utsouthwestern.edu > >>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > >>> Sanders, Jeanine (CDC/OID/NCEZID) > >>> Sent: Tuesday, March 24, 2015 12:30 PM > >>> To: Sue; Timothy Morken > >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >>> Subject: RE: [Histonet] BS in Histotechnology > >>> > >>> I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >>> teach any trained dog how to section histology will never have the > >>> recognition those of us that have studied and trained deserve. >
[Histonet] Another question: C4d and preeclamptic placentas
Hello Netters, plus Matt and Keith ;-) I'm picking the collective brain of the Histonet... I've found some literature that suggests that up to 50% of preeclamptic placentas that resulted in a negative outcome have diffuse C4d staining when using IHC. Has anyone tried C4d IHC or IF on preeclamptic placentas? Tomorrow's Fridayso have a nice weekend everyone! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C3d?
Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Positive Control for IF?
Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering. Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BS in Histotechnology
I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we started > our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any monkey > >could be trained to do my job". I basically did not take the job I was > >interviewing for at the time. At least the next interview went a lot > >better and I did take the job. > > > >Pam > > > >-Original Message- > >From: histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, > >Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>as long as many pathologists think you can teach > >any trained dog how to section histology will never have the recognition > >those of us that have studied and trained deserve. > > > >-Original Message- > >From: histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. Even > >though there is an increase in automation in pathology the hands on of a > >histologists is most important. The fact that hospital still consider a > >lower entry job is the reason there are not more of us. It is quite > >frustrating. > > > >Sue > >TJUH > >___ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >-- > >Confidentiality Notice: This e-mail message, including any attachments, > >is for the sole use of the intended recipient(s) and may contain > >confidential and privileged information. Any unauthorized review, use, > >disclosure or distribution is prohibited. If you are not the intended > >recipient, please contact the sender by reply e-mail and destroy all > >copies of the original message. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS control
Thank you to all who volunteered ideas and control blocks. Of course, the control must be of human origin, so they are digging through their files to find a nice fungusy patient tissue for me. Darn those CAP regulations, they take all the fun out of it. I still want to try an orange or Slim Jim or hotdog. Have a nice day, Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS controls?
Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. Ideas? Thanks in advance. Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding Question
Thanks to all who submitted their input. Since the "experts" were going for lean in the new histology space, more than likely the embedding stations will go in there. It *is* more efficient to have it set up this way. I just worried about the itsy bitsy GI biopsies not embedding well. A majority of people who replied had experience with this type of lab layout and the tissue samples did not suffer any as a result of getting a tour of the hallway. Many had to travel much farther than 200 feet. As suggested by many, I will pass along the very good idea of using a sturdy bin with a nice fitting lid for transport. I will think of it this way: better to have the chance of dropping the basket and cassettes and getting wax on the floor, than to drop the basket and cassettes in NBF while traversing the hallway. Hazmat, here we come, if that were to spill. Happy Thursday! Paula On Thu, Mar 12, 2015 at 2:07 PM, Jeffrey Robinson < jrobin...@pathology-associates.com> wrote: > This certainly does not sound ideal to be moving tissue blocks around the > building. If this situation cannot be changed then perhaps a plastic bin > with a snap on lid could help minimize the risk of losing blocks during > transport. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 9:47 AM > To: Hannen, Valerie > Cc: HistoNet > Subject: Re: [Histonet] Embedding Question > > Why the change? Space. There is a larger space available for histology. > > It would be optimum to either move the processors into the new space or > leave the embedding centers where they are now. > > I mentioned did bring up about the samples/wax freezing in transit and the > reply was "the experts didn't mention anything about that" > > The experts may not have been experienced histotechs. > > Paula > > On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie < > valerie.han...@parrishmed.com> wrote: > > > I do not think it to be a good idea. > > > > 1) What is the possibility of a "trip and fall" in transit? > > Cassettes flying everywhere, possibility of losing any? > > > > 2) Dripping paraffin on a hallway floor-- a slip and fall scenario > > for other people using the "much used" hallway. > > > > 3) As Tom stated.. not a lean system/process. > > > > Just my two cents! > > > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > > Section Chief, Histology > > Parrish Medical Center > > 951 N. Washington Ave. > > Titusville,Florida 32796 > > T: (321)268-6333 ext. 7506 > > F: (321) 268-6149 > > valerie.han...@parrishmed.com > > www.parrishmed.com > > > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, > > Thomas > > Sent: Thursday, March 12, 2015 10:32 AM > > To: Paula Sicurello; HistoNet > > Subject: RE: [Histonet] Embedding Question > > > > Not what I call a lean system. Why the change? > > > > Tom > > > > > > Tom Podawiltz HT (ASCP) > > AP Section Head > > LRGHealthcare > > 603-524-3211 ext: 3220 > > > > > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula > > Sicurello > > Sent: Thursday, March 12, 2015 9:29 AM > > To: HistoNet > > Subject: [Histonet] Embedding Question > > > > It has been proposed to move the embedding centers to a room about 210 > > ft away from the tissue processors. > > > > The trip from processor to embedding center would take over 2 minutes > > and require the histotechs to carry the baskets full of cassettes down > > a much used hallway. > > > > Opinions? > > > > Do you feel this is a good idea-yes or no and why? > > > > Thanks in advance, > > > > Paula > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and > > confidential information intended only for the use of the recipient(s) > > named above. If you are not the intended recipient, you may not > > print,dist
Re: [Histonet] Embedding Question
Why the change? Space. There is a larger space available for histology. It would be optimum to either move the processors into the new space or leave the embedding centers where they are now. I mentioned did bring up about the samples/wax freezing in transit and the reply was "the experts didn't mention anything about that" The experts may not have been experienced histotechs. Paula On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie < valerie.han...@parrishmed.com> wrote: > I do not think it to be a good idea. > > 1) What is the possibility of a "trip and fall" in transit? Cassettes > flying everywhere, possibility of losing any? > > 2) Dripping paraffin on a hallway floor-- a slip and fall scenario for > other people using the "much used" hallway. > > 3) As Tom stated.. not a lean system/process. > > Just my two cents! > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.han...@parrishmed.com > www.parrishmed.com > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas > Sent: Thursday, March 12, 2015 10:32 AM > To: Paula Sicurello; HistoNet > Subject: RE: [Histonet] Embedding Question > > Not what I call a lean system. Why the change? > > Tom > > > Tom Podawiltz HT (ASCP) > AP Section Head > LRGHealthcare > 603-524-3211 ext: 3220 > > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 9:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 ft > away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes and > require the histotechs to carry the baskets full of cassettes down a much > used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication > in error, please notify the sender by return e-mail and delete this message > and any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > == > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > == > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding Question
Hi Tim, There are several embedding events through-out the day, though mostly in the wee hours of the morning. The embedding centers would be in the same room as the microtomes (another question about those tomorrow). I worry about the small (GI, needles, etc) biopsies freezing before they reach the embedding stations. In my experience, once they freeze they get this outer wax coating (like a permeability barrier) which doesn't melt when placed in the dry (no paraffin inside) but hot, holding bin. They just don't seem to embed that well and have a tendency to drop out of the sections when cutting. Has anyone else had that happen? Paula On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy wrote: > Paula, > How many times per day? > Is the embedding close to the cutting area? > > Of course any extra walking is a problem, especially in busy areas. Is > this a non-patient area (hopefully!)? Any restructuring should be to move > things closer together, not further away! > > Having said that, If it comes to that I would be more concerned about > embedding proximity to the cutting area since having embedding near cutting > enhances workflow and cross coverage. If you don't unload processors very > often then having them distant might not be too bad. Not ideal, but not a > necessarily a deal killer. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 6:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 ft > away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes and > require the histotechs to carry the baskets full of cassettes down a much > used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Question
It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How dark is dark enough?
Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And other crazy stuff.
I forgot to mention: dirt Happy Friday Paula On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell wrote: > Orangutan testicle macro section and alligator jawbones, not my best work, > very humbling, after 52 years in the business. > Shirley > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had > to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte > that was nearly microscopic. I'll just say it took a LOT of thick sections > too face down to it without actually cutting through it. > > > Open the floodgates > > Tim Morken > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol > methacrylate (gma) it is much harder and would give better sections, we > have done zebra fish and several other harder tissues including calcified > bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > prueg...@hotmail.com > > > > > From: r...@psu.edu > > To: classic...@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Sat, 3 Jan 2015 23:15:33 + > > Subject: RE: [Histonet] cutting honey bees > > CC: > > > > I sectioned and stained honey bee and yellow jacket stingers years ago. > They wanted to show the difference between the stingers. I wasn't sure > what to do so I processed and handled like everything else. I was able to > get some good sections. I put 6 stingers in each block and cut several > sections figuring there should be at least one good stinger in each block > and it worked. > > Roberta Horner > > Penn State University > > Animal Diagnostic Lab > > > > From: histonet-boun...@lists.utsouthwestern.edu > > [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg > > [classic...@gmail.com] > > Sent: Saturday, January 03, 2015 6:08 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] cutting honey bees > > > > Has anyone had experience embedding and cutting honey bees. I am sure > > there are some issues with the harder exoskeleton. Would that have to > > be dissected away first. I am considering helping a student with a > > science fair project on bees. > > > > Douglas Gregg > > Veterianary pathologist > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones wrote: > We did a goldfish once, interesting microscopically and difficult for > peeling (lots of keratin?) > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > mjo...@metropath.com > > > > > On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: > > >You crazy research people...OK, so what is the craziest thing you ever > >had to cut, or were asked to cut? > > > >For me, not too bad, but embedding for EM and sectioning a single oocyte > >that was nearly microscopic. I'll just say it took a LOT of thick > >sections too face down to it without actually cutting through it. > > > > > >Open the floodgates > > > >Tim Morken > > > >-Original Message- > >From: histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy > >Ruegg > >Sent: Tuesday, January 06, 2015 11:13 AM > >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > >Subject: RE: [Histonet] cutting honey bees > > > >for the whole bee I probably would process and embed it in glycol > >methacrylate (gma) it is much harder and would give better sections, we > >have done zebra fish and several other harder tissues including calcified > >bone in GMA. > > > >Cheers, > >Patsy > > > >Patsy Ruegg, HT(ASCP)QIHC > >Ruegg IHC Consulting > >40864 E Arkansas Ave > >Bennett, CO 80102 > >H 303-644-4538 > >C 720-281-5406 > >prueg...@hotmail.com > > > > > > > >> From: r...@psu.edu > >> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu > >> Date: Sat, 3 Jan 2015 23:15:33 + > >> Subject: RE: [Histonet] cutting honey bees > >> CC: > >> > >> I sectioned and stained honey bee and yellow jacket stingers years ago. > >> They wanted to show the difference between the stingers. I wasn't sure > >>what to do so I processed and handled like everything else. I was able > >>to get some good sections. I put 6 stingers in each block and cut > >>several sections figuring there should be at least one good stinger in > >>each block and it worked. > >> Roberta Horner > >> Penn State University > >> Animal Diagnostic Lab > >> > >> From: histonet-boun...@lists.utsouthwestern.edu > >> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg > >> [classic...@gmail.com] > >> Sent: Saturday, January 03, 2015 6:08 PM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] cutting honey bees > >> > >> Has anyone had experience embedding and cutting honey bees. I am sure > >> there are some issues with the harder exoskeleton. Would that have to > >> be dissected away first. I am considering helping a student with a > >> science fair project on bees. > >> > >> Douglas Gregg > >> Veterianary pathologist > >> > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >___ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
Registered 4 years Doing histology 30 years. On Thu, Aug 7, 2014 at 5:19 PM, Jennifer MacDonald wrote: > 31 years > > Jennifer MacDonald > > > > From: "Douglas Porter" > To: > Date: 08/07/2014 11:36 AM > Subject:[Histonet] On the lighter side... > Sent by:histonet-boun...@lists.utsouthwestern.edu > > > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > <mailto:doug.por...@caplab.org> doug.por...@caplab.org > > <http://www.caplab.org/> www.caplab.org > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clinical EM Labs?
Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC imaging systems
Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Second Question of the Day: -80 degrees rationale
Hello Again, Gayle and Bill have provided the most succinct answers to the question of the day. Second Question: Is there a lower end, say -70 or -75, that provides the same protection as -80? I'm asking all this because I've inherited an old -80 freezer and would like it to last as long as possible. The lab that had this freezer previously had an acceptable range of -70 to -90. Thanks in advance, again, oh wise ones. Paula -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. On Wed, Nov 20, 2013 at 10:58 AM, WILLIAM DESALVO wrote: > Here is my stab at why -80 C. > > Temperatures between 0°C and -25°C, the enzymatic activity of cells is > only slowed but remains active. Below -40°C physiochemical exchanges are > frozen. Cellular morphology is preserved at -80°C. Shelf life of tissue > increases as the temperature drops. Once you get below -80°C you will > need cryoprotectors and when you use cryoprotectors, temperatures must be > below -130°C and will go as low as -196°C (liquid nitrogen). Antibodies > and proteins in solution are stable at -20°C. > > *William DeSalvo,* *BS HTL(ASCP)* > > > Date: Wed, 20 Nov 2013 09:25:54 -0500 > > From: pat...@gmail.com > > To: histonet@lists.utsouthwestern.edu; microsc...@microscopy.com > > CC: > > Subject: [Histonet] -80 degrees rationale > > > > > Hello My Fellow Listers, > > > > The question of the day is: What is the rationale for storing frozen > > biopsies at -80 degrees? > > > > I have seen protocols that range in temperature from -40 to -80 degrees. > > > > Was -80 selected because that was the lowest freezers could go back in > the > > day? > > > > Awaiting your chilly responses! > > > > Thanks in advance, > > > > Paula > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] -80 degrees rationale
Hello My Fellow Listers, The question of the day is: What is the rationale for storing frozen biopsies at -80 degrees? I have seen protocols that range in temperature from -40 to -80 degrees. Was -80 selected because that was the lowest freezers could go back in the day? Awaiting your chilly responses! Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] -80 freezer question
Dear Netters, What is the rationale for storing frozen patient samples at -80? Is there a range that keeps the sample viable but not as cold? Our -80 (which we just inherited) is struggling and I would like to raise the temperature a smidge to ease the stress on the compressor. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Remove OCT from frozen tissue block
Hi Benoit, You can just let the OCT thaw at room temperature then blot away the melted OCT. To speed up the thawing process carefully cut away as much of the OCT as you can with a scalpel or razor blade and then let it thaw. I hope this information is helpful. Paula On Thu, Oct 31, 2013 at 3:57 AM, Benoît Delatour wrote: > Dear histoneters, > We got mice brains that were initially sucrose-cryoprotected and then > embedded in OCT - flash frozen and stored at -80°C. These tissues were > initially prepared for subsequent cryostat sectioning but due to technical > considerations we need to cut them using a sliding microtome to get thick > (40µm) floating sections. We therefore would like to remove OCT before > cutting. As OCT is water soluble it is expected that placing the OCT blocks > in buffer would help removing the embedding media but thawing the tissue > and then re-freezing it on the microtome stage might not be the best way to > proceed (expected formation of ice crystals with known associated > histological artefacts)... > We would appreciate any alternative solutions. Thanks! > Benoît > > > __**_ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome Blade safety, in or out when not in use?
Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, > When you finish work, do you leave your blade in the microtome behind the > blade guard or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] External UV for a Leica 1850?
Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] More about the good old days..
I had a co-worker who would drink CocaCola mixed with the sealed, glass flask 100% ETOH every Friday. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Fri, Sep 20, 2013 at 2:43 PM, Victor A. Tobias wrote: > I gave plenty of thought to the ETOH going down the drain. > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtob...@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > = > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com > Sent: Friday, September 20, 2013 11:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] More about the good old days.. > > And the stuff we poured down the sink without a second thought > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: bunsen burner at the embedding center
I worked for the Navy Hospital in San Diego, way back in the day, and we did an extraction with fuming HCl (straight, 100% HCl for folks who didn't know what fuming meant). Several people would get nosebleeds every time they performed the extraction. I finally got one of those fans that looks like an airplane propellor in a cage and pried open the painted shut windows. I dared anyone to stop me, no one did! 1 was quite gutsy for a 22 year old. The Petty Officers had me make 10N NaOH and forgot to tell me how hot it got. I almost dropped it when I picked it up. Plus I had to swim to work, both ways in a rip tide-battleing sharks and jelly fish, while sloshing around in an earthquake! It is San Diego after all. Happy Friday! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen wrote: > yet here we are..how much of what they tell us is hyperbole? > > > Date: Fri, 20 Sep 2013 14:50:24 + > > From: mucra...@comcast.net > > To: rsrichm...@gmail.com > > Subject: Re: [Histonet] Re: bunsen burner at the embedding center > > CC: histonet@lists.utsouthwestern.edu > > > > > > > > I actually knew the person you are speaking of and it was his favorite > trick. We should be careful these younger people are using gloves for > everything now to protect themselves. W hen I started in Histology even > the pathologists cleaned the paraffin off their hands with xylene and > encouraged everyone to do the same . We used so many things that are now > not even allowed to be open on this planet and did know how dangerous or > serious the possible issues could be over time. > > > > > > Pam Marcum > > > > > > > > - Original Message - > > From: "Bob Richmond" > > To: "Histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > > Sent: Friday, September 20, 2013 9:35:55 AM > > Subject: [Histonet] Re: bunsen burner at the embedding center > > > > I distinctly remember when I was a resident at Johns Hopkins in the late > > 1960s that the histotechs would smoke while staining and coverslipping > > without much ventilation. When I suggested to the chief technologist (who > > later died of smoking related disease at 65, but at least he hadn't set > > himself afire) that this wasn't such a good idea, he responded by > stubbing > > out a lighted cigarette in a Stender dish full of xylene (apparently you > > can do this trick with gasoline also, but don't try it at home please). > > > > Buffering formalin was prohibited back then, and they removed the > formalin > > pigment by passing the sections through a concentrated solution (20 or > 30%) > > of picric acid in acetone. I'm glad he didn't try the cigarette trick in > > THAT Stender dish. > > > > Fast-forward nearly half a century, and in the three labs I'm working in > > I'm still grossing formalin-fixed tissue with minimal ventilation, but at > > least people aren't allowed to smoke in the lab any more. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Bunsen Burner
No open flames allowed. Bill, If you use nitrile gloves, the sticky buns don't stick! -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Sep 19, 2013 at 12:17 PM, jeff lowen wrote: > yeah and these darn gloves get in the way of drinking my coffee and > enjoying my sticky bun. > > > From: billodonn...@catholichealth.net > > To: valerie.han...@parrishmed.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 19 Sep 2013 15:56:24 + > > CC: > > Subject: [Histonet] RE: Bunsen Burner > > > > No open flames - which makes it hard to light our cigars. (Just > kidding but I remember when...) > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > > Sent: Thursday, September 19, 2013 10:04 AM > > To: Histonet Post (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] Bunsen Burner > > > > Hi all.. > > > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > > > What is the consensus?? > > > > > > Thanks, > > > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > > Histology Section Chief > > Parrish Medical Center > > 951 N. Washington Ave. > > Titusville, Florida 32976 > > Phone:(321) 268-6333 ext. 7506 > > Fax: (321) 268-6149 > > valerie.han...@parrishmed.com > > > > > > = > > "This email is intended solely for the use of the individual to whom it > is addressed and may contain information that is privileged, confidential > or otherwise exempt from disclosure under applicable law. If the reader of > this email is not the intended recipient or the employee or agent > responsible for delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or copying of this > communication is strictly prohibited. If you have received this > communication in error, please immediately delete this message. Thank you" > > = > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EPIC Beaker
See if you can get your Beaker people to talk to the Beaker people who are working on the Duke University Health System-ClinLabs AP build. We are taking it one step at a time and the AP module is being built separately from the CP build. Beaker is taking over 18 months to build, we have tentative go-live for July 2014. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Wed, Sep 4, 2013 at 10:15 AM, Pam Marcum wrote: > > > > I know asked this a couple of months ago however; we are just getting > ready to work with the EPIC people on the Histology/IHC/Slide & Block/Gross > Room issues we need in AP. I am looking for any advise or recommendations > anyone who is running Epic Beaker now or is in the process as we are to > implement it in the next 6 months or so. I would prefer this is off line > if anyone resonding is OK doing it that way. If others want the info then > please to Histonet will work too. > > > > We are having issues getting our IT group and Epic to understand AP is not > the same as CP. > > > > Thank You, > > Pam Marcum > > UAMS Histology Supervisor > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome aligner
I used an even cheaper version of the aligner. My microtome service engineer suggested an "L level" or a "T" level that you can buy at Home Depot. The L looks just like an L, it is actually a right angle measuring device, used to make sure the corners or something are straight at 90 degrees. You take the knife holder out and place one side of the L on the knife holder place and the other against the block face. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) < algra...@email.arizona.edu> wrote: > They do work. I saw one at NSH some years ago and came home and had the > guys in our shop make one for me exactly like the one I saw and it cost > next to nothing. We bought the little round level at home depot. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gross Room cleaner
Dispatch works well too. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < amber.mcken...@gastrodocs.net> wrote: > > What do you guys use to clean your Gross Room utensils? I bought Lem'in > Lift from Mantek in the past to soak the rulers, scissors, tweezers, > etc...Thanks! > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
Hi Julia, Thanks for the information. Can you tell me why you store it in the refrigerator? Paula Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, Apr 16, 2013 at 1:39 PM, Celebre Julia wrote: > We store our open bottle in an explosion proof fridge and unopened at room > temp in a flammable storage cabinet > > Julia Celebre Sr MLT > Anatomic Pathology > Hamilton General Hospital > 905-527-4322 ext 46179 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, April 16, 2013 1:35 PM > To: HistoNet > Subject: [Histonet] Isopentane storage-the polling is open > > Hello HistoNetters, > > I have been having a discussion with my boss about whether or not to store > the isopentane (used for freezing muscle biopsies) in the refrigerator. (An > expensive, explosion proof one that I would have to buy.) > > Her only experience is with the isopentane stored in the refrigerator, my > experiences are with storing it at room temperature. > > What do you all do? Room temp or refrigerated? > > Any and all comments are greatly appreciated. > > Thanks! > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory Duke University Health > System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 > P: 919.684.2091 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This information is directed in confidence solely to the person named > above and may not otherwise be distributed, copied or disclosed. Therefore, > this information should be considered strictly confidential. If you have > received this email in error, please notify the sender immediately via a > return email for further direction. Thank you for your assistance. > -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isopentane storage-the polling is open
Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Possible CJD and decontaminating Leica ASP300s
Lori, You need to contact the National Prion Disease Center at http://www.cjdsurveillance.com/ They will tell you if there is a way to decontaminate anything that may have come in contact with the suspected CJD case. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Apr 5, 2013 at 1:37 PM, wrote: > We processed a cell block last week and we are now just being notified > that it MAY be a CJD case. Leica tells me there isn't a way to > decontaminate my tissue processor. Has anyone else been thru this, and > isn't there anything to do besides buy a new Tissue Processor? > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Temperatures
We have NIST traceable digital thermometers that can have the min/max reset. We have been told that if you reset on Friday and check on Monday morning the min/max & it is in range-put an OK by the date. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Feb 8, 2013 at 9:30 AM, wrote: > We have the clinical lab check our friges and freezers on the > weekends..everything else is off. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, > Beatrice > Sent: Friday, February 08, 2013 7:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temperatures > > I'm looking for a fix to our problem of no temperatures being taken on the > weekends. We are closed and this is creating an issue. Our processors are not > running until Sunday night but the paraffin in both the processors and > embedding center are kept molten. Any help would be greatly appreciated > > Beatrice L. Sullivan HT(ASCP)HTL > Corporate Histology Manager > Virtua, Voorhees > 856-247-3144 > > > This message, and any included attachments, are from Virtua Health or its > related affiliates and is intended only for the addressee(s). The information > contained herein is privileged, proprietary or may include confidential > information and/or protected patient health information. Any unauthorized > review, forwarding, printing, copying, distributing, or otherwise > disseminating or taking any action based on such information is strictly > prohibited. If you have received this message in error, or have reason to > believe you are not authorized to receive it, please delete this message > promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. > > Thank you > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 4% Paraformaldehyde
Rhonda, What Tim says is true, it all depends on what the tissue is going to be used for. Provide more detailed information to get a more detailed answer. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Feb 1, 2013 at 1:17 PM, Garlick, Rhonda wrote: > > I got one response from my question below. Does anyone else have hard and > fast rules with regards to the length of time tissues should/can be stored > in 4% Para? > > Thanks, Rhonda > > > > > On 1/22/13 1:08 PM, "Garlick, Rhonda" wrote: > >>I've been doing some on-line searching with regards to the length of time >>tissues can remain in 4% Paraformaldehyde, but can't seem to get any >>definite answers. Can anyone help. >> >>Thanks, Rhonda >>Univ. of Denver >>Denver, CO >>___ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No expiration dates on chemicals
We keep ours no longer than 5 years. If there is no expiration date on the bottle when we receive it, we mark it as expired 5 years from date of receipt. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Thu, Jan 31, 2013 at 12:36 PM, PicheGrocki, Jessica wrote: > Hi All, > > Just wondering what everyone is doing in regards the ANP question involving > expiration dates on chemicals and reagents? Is everyone getting rid of old > chemicals that have been opened for 10 years or more? > > Please advise. > > Have a good day and thank you, > > Jessica Piche', HT (ASCP) > Waterbury Hospital Histology Lab > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential > information that is legally privileged. This information is intended only for > the use of the individual or entity named above. The authorized recipient of > this information is prohibited from disclosing this information to any other > party unless required to do so by law or regulation. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution or action taken in reliance on the contents of these documents > is strictly prohibited. If you have received this information in error, > please notify the sender immediately and delete these documents. Copyright > (c) Waterbury Hospital > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ATPase without barbital buffer... info
Hi Tim,
Thanks for finding those protocols for us!
Perhaps we can all move away from using a controlled substance (what a
nightmare to purchase).
Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: 919.684.2091
On Wed, Jan 30, 2013 at 2:08 PM, Morken, Timothy
wrote:
> Well, I finally found the original papers on the subject of non-barbital
> buffers for ATPase from the Histonet posting below from Tony Henwood:
>
> The reference given for the procedure was : "Loughlin, M. (1993). Muscle
> biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79."
>
> But that is a book and one not available through our store. So I went online
> and was able to get the book for $2.00. yes, Two Dollars!
>
> Now I can give everyone the original references in the book. We have not
> tried this yet but plan to this year.
>
> The book actually has several ATPase methods that do not use barbital buffer
> on pages 77 - 82 (1993 edition).
>
> (One note. The page reference given by Tony's posting below seems to be the
> Brooke and Kaiser pH 4.3 and 4.6 method on page 81-82 combined with the Round
> pH 9.4 method on pp 80-81
>
>
> 1) Tris incubating buffer pH 9.4 (Louglin pp 77-79)
>
> a. Hayashi and Freiman, An Improved Method of Fixation for Formalin
> Sensitive Enzymes with Special Reference to Myosin Adenosine Triphosphatase,
> J Histochem & Cytochem, 1966 14: 577-581
>
> 2) Glycine incubating buffer pH 9.4 (Laughlin pp 80-81
>
> a. Round, et. Al. Quick, Simple and Reliable Method for ATPase in Human
> Muscle Preparations. The Histochemical Journal, Nov 1980, Vol 12, Issue 6, pp
> 707-710
>
> 3) Acetate incubating buffer pH 4.3 and 4.6 ("reverse" ATPase) (Laughlin
> pp 81-82
>
> a. Brooke, M.H. and Kaiser, K.K., Muscle Fibre Types: How many and what
> kind? Archives of Neurology, (Chicago), 1970, 23, 369-379
> Another non-barbital method is:
> Khan, M.A., et al, A calcium-citro-phosphate technique for the histochemical
> localization of myosin ATPase, Stain Technology, 1972, Vol. 47, No. 6, pp.
> 277-281.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> ATPase with sodium acetate buffer
>
> Tony Henwood AnthonyH <@t> chw.edu.au
> Sun Aug 20 18:14:40 CDT 2006
> Here is our method using acetate buffer in place of barbiturate buffer:
>
> Adenosine Triphosphatase (ATPases) [Note: this is the Brooke and Kaiser 4.3
> and 4.6 method
> combined with the Round 9.4 method. tm]
>
> Use
>
>Demonstration of muscle fibre types.
>
> Underlying Principle
>
> The principle relies on the ability of the enzyme to remove the terminal
> phosphate from the ATP,
> which then combines with calcium in the incubation solution to form an
> insoluble calcium
> phosphate. Cobalt is then exchanged for the calcium, which, after reaction
> with ammonium
> sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme
> activity.
>
> Fixation and Sectioning
>
>Air dried unfixed 8µm cryostat sections
>
> Reagents
>
> 1.1% calcium chloride
> a.Calcium chloride5.0 g
> b.Distilled water
> 500ml
>
> 2.2% Cobalt chloride
> Warning: Suspected Carcinogen - see MSDS
> a.Cobalt chloride
> 10.0 g
> b.Distilled water
> 500 ml
>
> 3.1% Ammonium sulphide
> Warning: Flammable liquid, Irritant, Toxic stench - see MSDS
> a.20% ammonium sulphide 0.5 ml
> b.Distilled water
> 9.5 ml
>
> 4.Acid Pre-incubation medium
> a.0.2M Sodium Acetate
> i. Sodium Acetate Anhydrous 0.82 g
> ii. Distilled water
>50 ml
> iii.
> b.0.2M Acetic Acid
> i. Glacial Acetic Acid
> 0.6 ml
> ii. Distilled water
>50 ml
>
>
> 5.0.2M Acetate Buffer: pH 4.3 pH 4.6
> a.0.2M Sodium Acetate 11 ml 18 ml
> b.
Re: [Histonet] RE: Permanent Fluorescent Mounting Media
James, When I was at MCV we used Crystal Mount for our frozen IF's (skin, kidney, etc.) You put a few drops on, place it in the oven for a little bit (I do not recall the temperture of the oven) and you had a nice hard cover over your sections. I hope this helps. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, Jan 15, 2013 at 1:25 PM, James Watson wrote: > Has anyone used hardmount mounting medias for fluorescence in a high volume > situation (up to 120 slides a day at times) when the slides are scanned? We > have tested BioCares, Thermofishers, and Vectashields. We found that we > liked the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant > worked, but with scanning we saw some stains were out of focus. The > VECTASHIELD HardSet Mounting Medium with DAPI gave us a higher percentage of > fading and out of focus images. > > Our issue with the hardmount and scanning is that we see one edge of the > slide in focus and the other out of focus frequently. We are working on our > mounting technique to see if this can be corrected by modifying our > coverslipping technique. > > Any suggestions would be appreciated. This does not make your fluorescence > permanent since most fluorochromes fade with age. > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Tel858-332-4647 > Fax 858-812-1915 > jwat...@gnf.org > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suresch, > Donna L. > Sent: Tuesday, January 15, 2013 10:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Permanent Fluorescent Mounting Media > > Hello Histonet, > Anyone know of a non-aqueous permanent mounting media for use with > fluorescent IHC? > > Donna L. Suresch > Senior Imaging Research Scientist > Merck Research Laboratories > Department of Imaging - West Point Campus > Mail Stop: WP44KOffice: WP44-H129 > 770 Sumneytown Pike > PO Box 4 > West Point, PA 19486-0004 > Phone: 215-652-7349 > Fax: 215-993-6803 > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely for > the use of the individual or entity named on this message. If you are not the > intended recipient, and have received this message in error, please notify us > immediately by reply e-mail and then delete it from your system. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 88305TC starting to hit the fan...
Sure they want more physicians. My husband was forced to leave medical school when he became sick (they wouldn't give him a medical leave of absence). No other medical school would touch him after that. They thought something was fishy, his transcript reads "medical leave of absence" which wasn't true. The other shcools can't figure out why the school he was in wouldn't take him back. If he wanted to retake the MCAT and apply cold, they told him he could do that. Medicine is a self limiting profession. The AMA determines how many doctors are availabe to keep a limited resource, limited. Thus the $280/hour radiologist. You leave with close to $200,000 in debt if attending a private school. Primary care pays, in most regions, under $100,000/yr. When you have that kind of debt, lower paying primary care jobs aren't enticing. My bitter two cents. Paula On Mon, Nov 19, 2012 at 4:25 PM, Webster, Thomas S. wrote: > CAP had a webinar last week about the cut. These are some very scary times. > For some reason the government has decided to shift wealth from specialists > to family practice. I am becoming more angry with the affordable care act > everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin and Operating Rooms
I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsi...@statlab.com | www.statlab.com > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Beaker module in EPIC
Hello all Listers, I've already asked the Histonet but I'm wondering about a different thing this time. Does anyone have experience with Beaker in conjunction with Anatomic Pathology, Surgical Pathology or the multiple other clinical labs that are in hospitals today? I work in Anatomic/Surgical Pathology which encompasses all the Clinical Laboratory services here at Duke. We have been told to determine what we need for the Beaker module to be useful to us. I've seen a brief demo. as to how it will work for the ordering clinicians (they can order every test STAT!). Please, send me any of the pluses or minuses that you've experienced using Beaker. Thanks, Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Epic Beaker module
Good Morning One and All, Does anyone out there have any experience with the Beaker module of the LIS system EPIC? We have tests that require tables and charts to be inserted into the final report, pathologists need to have a case queue and other items. If you are using Epic/Beaker- please let me know if your experiences and what if anything have you had Epic build into it for you. Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diamond Knives
Hi Philip, Diatome is the brand we use. Lately they have been having resharpening issues. We have had to send several back after resharpening because the knives left scratch marks on the thin sections. That said, they are friendly and helpful and have never complained about us returning one for additional resharpening. There are other brands on the market, I have not used them in a while so I cannot comment about those vendors. If you have anyother questions, feel free to ask. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Wed, Mar 14, 2012 at 10:42 PM, Philip Slakmon wrote: > Good Afternoon, > > I was interested in knowing people's opinions on the different diamond knives > on the market. Opinions could be based on quality, design, workmanship, > delivery, customer service/support, pricing, > > > Thank you, > > Philip > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] broken chuck holder
You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > Only when I used to work with the Incredible Hulk. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > yes...@comcast.net > Sent: 17 November 2011 11:41 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] broken chuck holder > > Does anyone have any experience with broken chuck holders? I have a tech > that has snapped 3 chuck holders in little over two years. I have never > seen this, has anyone else?? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This email is covered by the Kingston Hospital NHS Trust email disclaimer > http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Retirement
Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks wrote: > Agarose Gels! > ... Listen you whipersnapper Agarose is the easy way out. When I learned > it we used to have to make up our own polyacrylamide gels. That was after > having to walk to work up hill both ways in 30 feet of snow! > > (No nearer retirement) > Crotchety Amos > > > > Message: 7 > Date: Fri, 17 Jun 2011 13:24:12 -0400 > From: Emily Sours > Subject: Re: [Histonet] Retirement > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=UTF-8 > > Retirement? I think by the time I get to that point, social security will > have run out. > Then again, technology will be so advanced, I can tell stories about the old > days, where I logged on to the bbs by modem to post messages to my friends > and typed in my own html coding. > We didn't have google when I was young!! Our cameras used film! And you > couldn't see how bad your pictures were until you developed that film!! > There was no PCR to sequence your DNA, you ran an agarose gel and hoped for > the best!! You could drink the 100% ethanol, there was no denaturing! (okay > that was before my time) You could smoke in the lab while you sectioned > without gloves!! (okay that was too) > > Emily > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OT: How do you call...
This reminds me of a joke I learned as a kid. How to use analyze and anatomy in a sentence (song actually). "My analyze over the ocean, my analyze over the sea, my analyze over the ocean, so bring back my anatomy." :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Jun 13, 2011 at 6:18 PM, Sebree Linda A wrote: > Anatomic Pathology here. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Monday, June 13, 2011 1:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT: How do you call... > > I've figured out how to fund my approaching retirement! A poll! So... > is the correct term "Anatomical Pathology" or "Anatomic Pathology"? > Send $5.00 with your email reply and I'll have the down payment for > that tropical island with internet. Seriously - what is the consensus > and/or the correct usage? Figure I better get all these Deep Questions > answered while I have the chance. (P.S., I'm only kidding about the > $5.00 but I do take PayPal). > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] colloidion
Is there any reason why the pathologist can't used agar? It's very simple to use and non-toxic. -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, May 27, 2011 at 11:22 AM, Michele Carr wrote: > Hi everyone, I am just wondering if any of you out there use this chemical > called colloidion. Our pathologists want to use it for making cell blocks, > but > looking over the msds, it's not very safe. If you use it or have used it > could > you tell me how exactly you stored it and disposed of it. Also if you have any > procedure on how to use it to create cell blocks. > Thank you all for your help, > Michele Carr > Medical Laboratory Services > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Section position on slides
Hi Tanya, It's always best to train people to place the section in the middle of the slide. It depends if one has to put multiple sections on a slide. Learning how to do proper placement will help them when they have to cut controls for IHC that are often times placed on the top of the slide. Sections placed too close to the very bottom or top sometimes don't get stained or coverslipped. Too close to the edges and you can suffer wrap around or overlap, causing the section to be difficult for the pathologist to read. Boy howdy! They will let you know if they don't like the placement. Therefore, train for centering the section as best as possible. I hope this helps, Paula :-) On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem wrote: > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement of > the actual sections on the slides. These are the objectives I am looking to > answer. Is this information found in any publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm x > 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. “x” mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more widely > used in IHC, are there published guidelines / documentation on the usable or > diagnosable staining area on a 25mm x 75mm glass slide? For instance, would > you define that as the area under a traditional coverslip? Would this be > defined as the entire slide below the label? Or is this some distance from > all the edges of the slide? With some automated systems, it is near > impossible to get edge to edge staining. Is this acceptable? > > Thanks for any ideas. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome alignment
A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this:l\ l \ l\ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wrote: > > Since you have older microtomes, I suggest using an "alignment block" at > each microtome instead of purchasing the alignment tools. The tools can be > found on the web ttp://www.grale.com.au/products/view/804 , but they can > be expensive (as much as $700.00 each). If you have more than one > manufacturer for your microtomes, you will need to purchase one for each > brand. > > Try using your largest embedding mold and make a blank block for each > microtome. This can bee done first thing each morning. Use the block to > align the chuck each morning before cutting. If you see drift throughout the > day, add one or more checks during the day. Making a fresh block each day > gives you a good standard and keeps the variation down. > > I also suggest you look at your embedding method and make sure you have a > standardized procedure for all tissue types for orientation of tissue and > exact placement in the mold. Embed your tissue on one plane with as little > paraffin as possible on the bottom of the mold. Reducing variation at > embedding will greatly assist you in reducing the amount of "facing" > required to start producing sections and also reduce the need to align the > chuck to the block/tissue. > > William DeSalvo, B.S., HTL(ASCP) > Chair, NSH QCC > Prodcution Manager, Sonora Quest Laboratories > > > > > > From: sharon.davis-dev...@carle.com > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 17 Aug 2010 14:16:26 -0500 > > Subject: [Histonet] Microtome alignment > > > > We are having a continuing issue of too much tissue being cut off when > facing off a block for recuts. We have tried a couple of different methods > for aligning our microtomes without much success. Does anyone out there have > any advice on how to properly align them and what tool to use? Also, how > often do you perform this re-alignment? The majority of our microtomes are > older so more wear and tear and things move out of place more often. Any > help or suggestions will be greatly appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-dev...@carle.com > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!!
at i...@bangslabs.com for additional details! Phone: >> 800-387-0672, 317-570-7020. >> >> >> >> * >> >> _ >> >> From: JERRY SANTIAGO [mailto:sant...@bellsouth.net] >> Sent: Tuesday, August 17, 2010 1:53 PM >> To: undisclosed recipients: >> Subject: NEEDS URGENT HELP !!! >> >> >> >> >> >> Help! I'm sorry to be emailing you like this, but I am in need of some >> urgent assistance. As you have know, I've been traveling through Europe. >> But last night in Scotland, my worst fears came true. As we were headed >> back >> to the hotel, we were robbed but a crazy man with a knife. He took our >> bags, >> wallets, and phones. We have no credit cards or cash.Thankfully, we had >> left >> our passports in the safe at the hotel. We need to get back home, but we >> have no way to get to our flight in Germany that leaves for home next >> week. >> >> Right now our only option is to fly home from here. We need some money so >> we >> can purchase tickets to get to JFK, and then fly home from there. As soon >> as >> we get back home, I can repay you, I just need to get some cash to get >> there. If you can help, send me an email back, we have internet access >> from >> the lobby in the hotel. I'm sorry to bother you, I just didn't know how >> else >> to contact everyone quickly Thanks! >> >> >> >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Breezy lab/flyaway ribbons!
Hi Josie and Chris, I've worked in labs with similar problems. Breezes are nice outdoors but cause havoc with ribbons. If you can't get them to put in a door (which you'll probably have to lock since folks will walk in, shut the door and cause a breeze that way) then how about those fuzzy cubicle walls? The ones that are used in prairie dog farms (I mean open office situations ;-) ). Also, you'll need to install some type of baffle or other item that hangs from the air ducts and deflects the air across the ceiling instead of straight down. Good luck! Paula :-) On Tue, Jun 22, 2010 at 6:22 AM, Josie Britton wrote: > We have been having trouble with big breeze blowing our precious ribbons > out of our hands while cutting. We would like a door on the Histology > lab to cut down on the breeze of people walking by through the hall. > Our facilities want to find out what other people are doing to stop this > problem. We also have air ducts blowing down from above, which is not > helping the problem. We would like as many labs solutions as possible. > Our facilities have come up with all these crazy barriers that we would > have to move to walk around when we need to put our racks on the > stainer, answer timers, print more slides, use the oven, etc... > > > > Any input would be appreciated! > > > > Breezy girls, > > > > Josie Britton and Chris Braaten > > Cheshire Medical Center > > Keene, NH 03431 > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Best books for the HTL?
Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BSL-2 question
Hello Everybody out there in virus land, I am getting a room ready for BSL-2 level virus imaging. I can't get any information regarding how safe the cells are once the virus has been transfected (?) in to them. I have a BSL-2 level bio-hood, I have a room with a door and I have a brand, spanking new deconvolution with FRET and an incubator for live cell imaging. I need to know if the people in the room are safe from the virus once it's inside the cells. I can't get the air pressure changed in the room so I need to know what other precautions we might need to take. Thanks to all who can give me any pertinent information. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: OT: Early Friday Hour of Fuming
I also feel your pain. I have lab where I do electron microscopy (clinical {if
they'd send me any} and research), confocal microscopy and histology. I also
have added cryosectioning and will be adding a micro-CT and a deconvolution
microscope (working at a BSL-2 level) with no increase in hours. I'm 80% and
my lab will be shut down at the end of the year because I do not bring in
enough revenue.
Clinical or research-it appears there aren't enough of us and we aren't
appreciated. Have the CEO of your company send out a blanket e-mail mentioning
a reduction in staffing when everyone knows that I'm the only one, talk about
lack of job security.
Grrr, hey at least the sun is shining and there are blue skies!
Paula :-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
C-MI for your imaging needs.
--- On Mon, 9/21/09, Roberta Horner wrote:
> From: Roberta Horner
> Subject: [Histonet] RE: OT: Early Friday Hour of Fuming
> To: "Breeden, Sara" ,
> "histonet@lists.utsouthwestern.edu"
> Date: Monday, September 21, 2009, 7:36 PM
> That's alright whenever I take
> vacation I come back to all the work sitting here waiting
> for me. I just took 2 weeks off and it took 3 weeks to
> catch up so I feel your pain.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Breeden, Sara
> Sent: Monday, September 21, 2009 2:23 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] OT: Early Friday Hour of Fuming
>
> I have just found out this morning that I will be unable to
> attend NSH
> this year. This alone is unfortunate news for me but,
> interestingly
> enough, is not due to budget issues. The reason I am
> "fuming" this
> morning is not because there is no funding for my
> attendance (that was
> already in place), but because there is such a serious
> shortage of
> histotechs in this country that there is no one to cover
> me! A
> "traveler" is out of the question (that's a budget issue!);
> anyone that
> might be hiding and is not known to me to be available in
> our city would
> have to have about a month's lead time to get vetted for
> the position
> through the State. Anyone that thinks that histology
> is not important
> should try to fill a position when there is such a shortage
> of trained
> and qualified technicians. If we, as
> histologists, do not begin to
> make ourselves more visible and promote our profession as a
> worthy,
> fulfilling and job-secure life, more and more of us will
> find ourselves
> in this position. Thank you - I feel better for having
> Fumed. There is
> no need to reply as I know many of us are in the same
> boat. I was in
> the boat and lost my paddle!
>
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM 87106
>
> 505-841-2576
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EM processing
I have never heard of an EM lab making their own resin for embedding. We almost all buy the resin kits or the separate components to make either a Epon 812 equivalent or Spurrs. The amount of time it takes to process is usually tissue dependent. On average, it takes one day to process (if the tissue is 1mm cubed or less) and 2 days in the oven for the resin to polymerize. You can speed things up if you microwave process. If you have any questions, please feel free to contact me. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Sat, 8/29/09, histopa...@aol.com wrote: > From: histopa...@aol.com > Subject: [Histonet] EM processing > To: histonet@lists.utsouthwestern.edu > Date: Saturday, August 29, 2009, 3:36 AM > Does your lab make their own plastic > resin to process and embed specimens or do you buy it > commercially? > What is the average time it take to process a specimen? > > Thanks in advance to all who respond. > P.Eneff > OU Medical Center > Oklahoma City, OK > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Organizing of cassettes for processing
When I batted clean up and loaded the processors, the residents grossed in the specimens and placed them in a basket in a container of formalin with a lid. The grossing was done and the containers were kept on the downdraft table. When it came time to load the processors I put the cassettes in numerical order and in separate baskets (smalls like GI biopsies and needle biopsies went together in one basket, fatty samples like breast tissue in another basket, etc). I did this on the downdraft table and placed the cassettes in numerical order. This helped us to find out if samples were mislabelled (2 cassettes with the same number) or missing (samples labelled A-F and D was missing). This saved our bacon many times since I would make notes or ask the resident where the sample was or which was which. It also made it easier for the embedders since I placed the notes regarding the missing cassettes by the embedding stations. Plus they could embed the block in numeric order so the slides that needed to get out first were cut and stained first. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/26/09, ba...@gundluth.org wrote: > From: ba...@gundluth.org > Subject: [Histonet] Organizing of cassettes for processing > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, August 26, 2009, 8:38 PM > > Hi all - > > For those that do batch overnight processing, how do you > organize the > cassettes? > > Currently we have 2 path assistants that gross throughout > the day, and each > puts their cassettes as they are grossed into a bucket of > formalin. At the > end of the day a histotech drains the formalin off, rinses > the cassettes in > water, then manually puts the cassettes into order > according to our > worklist, with rush cases being put up front. The > baskets are then loaded > onto the tissue processor (Sakura VIP5 and VIP6). > > We are wondering if there are some other ideas of how to > streamline this > process. One thought was to have the cassettes > loaded/organized into the > tissue processing basket as they are grossed, but have a > concern about > formalin exposure while doing this. > > Any thoughts would be greatly appreciated. > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] water on slides?
I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting wrote: > From: Angela Bitting > Subject: Re: [Histonet] water on slides? > To: histonet@lists.utsouthwestern.edu, "Tina J. Hayes" > Date: Monday, August 24, 2009, 6:00 PM > We are having a very similar problem > too. We change our alcohols and Histoclear like mad, but are > having eosin bleeding out of the sections. I'm starting to > wonder if it is the mounting media on our glass > coverslipper. I ordered new bottle gaskets, so I guess I'll > see what happens then. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Hayes, Tina J." > 8/24/2009 12:41 PM >>> > We are having a problem with what seems to be water on our > slides. We > have had very high humidity levels in the air. It > seems that we look at > the slides before taking them to the pathology office, one > pathologist > reviews them and sees little or no water droplets, but as > they sit and > another pathologist reads them later, like the following > day, the > droplets are very apparent. > > > > We use Clearite-3. We have no xylene in our > lab. We also coverslip > with Permount. > > > > Is it possible that the clearite-3 and/or Permount is > absorbing moisture > from the atmosphere to the slides? > > And if so, do you have any suggestions for combating this > issue? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the > documents attached to it, if any) is confidential and may be > legally privileged. It is intended solely for the addressee. > Access to this message by anyone else is unauthorized. If > you are not the intended recipient, any disclosure, copying, > distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you > have received this message in error, please delete all > electronic copies of this message (and the documents > attached to it, if any), destroy any hard copies you may > have created and notify me immediately by replying to this > email. Thank you. > -Inline Attachment Follows- > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cleaning oil off objectives
I use a dog chewed old stick. On another note, how to clean off dried mounting media. I have several objectives where some goombahs dragged the objectives through wet mounting media. If y'all have any suggestions about removing dried mounting media. Send them my way. 4 of my 5 objectives are no longer objective after having their lenses clouded by the media. ;-) Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Fri, 8/21/09, Pamela Marcum wrote: > From: Pamela Marcum > Subject: RE: [Histonet] Cleaning oil off objectives > To: "'Rittman, Barry R'" , > histonet@lists.utsouthwestern.edu > Date: Friday, August 21, 2009, 12:43 PM > Really like the taser idea! I > could have used that for several people over > the years. > > Pam Marcum > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] > On Behalf Of Rittman, > Barry R > Sent: Thursday, August 20, 2009 10:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cleaning oil off objectives > > Adam > Hi > I would strongly recommend that the best approach is to > train the people > using the microscope, everyone is trainable although for > some this may be a > long learning curve. > The use of a taser with the later individuals is strongly > recommended! > > Several years ago the Zeiss representative in Iowa used the > expanded plastic > packing beads to wipe off the excess oil as he said this > was much more > absorbent for oil that lens tissue. > We have also seen the use of soft wood tips with oil that > is encrusted on, > on the understanding that the wood is much softer than the > lens.. Never > completely happy with that concept. > A lot depends on the type of lens that is being used. > Some lenses, especially older ones may have a coating that > is easily damaged > even by Q tips. > I would use lens paper first (don't be cheap skate with the > lens tissue) > then repeat using a small amount of lens cleaner. The > most difficult and > usually the most contaminated seem to be the 40 due to its > working distance. > Most of the lens cleaners have isopropyl alcohol and some > acetone. > If it really does not get all the oil after repeating a > couple of times then > can use acetone but don't flood the lens just use small > amounts and wipe > across the face. Follow this with lens cleaner and lens > paper. > Has always worked for me. This sounds a lengthy procedure > but only takes a > couple of minutes. > Hope that this helps > Barry > > > > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] > On Behalf Of Adam . > [anonwu...@gmail.com] > Sent: Thursday, August 20, 2009 7:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cleaning oil off objectives > > Hi all, > > You guys were so helpful on my last question, I'll ask > another. We have a > microscope shared by the floor with several objectives, and > it's pretty > common for the non-immersion objectives to get contaminated > with oil. I > asked the guy who is responsible for the scope about this. > He said that they > call someone from some company who carefully cleans the > objectives with > acetone and a Q-tip, which if done right works wonders but > if done wrong it > can damage the lenses. But he mentioned that the lenses are > usually > re-contaminated within a few weeks since so many people use > the scope, so > it's sort of a pointless endeavor. This system seems pretty > silly to me... I > feel like there must be an easier and cheaper way to clean > the lenses > without damaging them; I certainly don't want to be > responsible for damaging > a microscope that costs more than my yearly salary. What do > you recommend? > > Thanks, > Adam > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Support for core units
Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Core unit support
Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3
Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all "pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
You might still have your fingers, but are your fingerprints still the same? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: From: Bartlett, Jeanine (CDC/CCID/NCZVED) Subject: RE: [Histonet] Start Up Lab To: "Smith, Allen" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 4:32 PM Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > > > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > __
