[Histonet] Prion Scrapie IHC
Does anyone do the Scrapie Prion IHC testing on the Leica Bond? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vet lab accreditation
NAHLN Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -Original Message- From: Johnson, Carole via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 23, 2015 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vet lab accreditation Hello and Happy Almost Friday, This question is for the veterinary labs: Who is your accrediting body? Is it AAVLD, NAHLN and/or are you CLI, CAP, AABB, JCI ISO 15189 or Joint Commission? I appreciate your input. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica bond
So far so good. Been using it for a year now. No issues or grips that couldn't be worked out with Leica. They have been very supportive. Their field staff comes to assist you from start to finish if need be. Leica even has classes to train on their machine in IL; all expenses paid. Oh one thing, their annual PM costs is ridiculously high. Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -Original Message- From: Miller, Wendy [mailto:miller.we...@mhsil.com] Sent: Wednesday, July 08, 2015 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lab math: ihc dilution
To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabies tissue Fixed in Formalin.
Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Edit protocol for Shandon Gemini Varistainer
Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list. I'm not sitting in front of my stainer right now to walk you through screen by screen but that is the whole idea behind it. Delete it, edit it, then add it back. (When you delete it, it's not deleted from the stainer its only deleted from being an active stain protocol.) I hope this helps a little. If you need further help, let me know I'll go to my machine and get the step by step info for you. You can also call their customer service # and they should be able to walk u thru it. Thanks, Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
Thank you All! Sheryl Stephenson | Histology Technician -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, October 03, 2013 9:43 AM To: Watson, Linda; Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-Original Message- >From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- >boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-Original Message- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT HistoDeck question...
Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Possible continuing automatic staining problem
What do you mean by "muted"; can you give a little more description? And what type of tissue, and is this H&E? Sheryl Stephenson | Histology Technician -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Sent: Saturday, March 16, 2013 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Possible continuing automatic staining problem Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope..Please any suggestions out there, Dr & histotech's very frustrated. Lisa A Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ASCP HT exam
C*O*N*G*R*A*T*U*L*A*T*I*O*N*S!! woohoo. Sheryl Stephenson | Histology Technician -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar Sent: Thursday, June 14, 2012 6:03 PM To: Histonet Archive Subject: [Histonet] ASCP HT exam Hello there histonetters. Some of you may remember some previous posts I've put up in regards to employment and taking the HT exam. I sat for my exam on Monday and..drum roll please I PASSED :):):):) I'm on the move to the bay area specifically Berkeley, and will be there by Sunday. So any info on jobs in that area would be greatly appreciated!!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome
If you want to stay with Leica; they have a newer model called RM2255 it has the manual and automatic rotations. Sheryl Stephenson | Histology Technician -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, June 08, 2012 10:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome Hi histonetters! We are a molecular biology lab with a histology facility (we do whole FFPE tissue sections to build TMAs and then TMA sectionning). We currently have a Leica RM2125 in the lab, but want to acquire a new manual microtome, mainly as a safe because we depend a lot on tissue sectioning. Could anyone give me some advice/suggestion on which microtome to buy? We only use the regular cassettes. Best regards, Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent rotation
We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct: 908.947.1624 sstephen...@lifecell.com 732. 939. 3037 Cell www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leitz 1512
Yeah, a brand new Leica RM 2255.:D Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct: 908.947.1624 sstephen...@lifecell.com www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Smallwood Sent: Sunday, May 27, 2012 4:46 PM To: Histonet Submissions Subject: [Histonet] Leitz 1512 Does anyone have a resource for parts for this 40 year old microtome ?? Thanks John Smallwood ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding
That was my initial thought, check your paraffin. I could be coming from the paraffin.. or whatever you are cleaning with make sure its dust free/debris free. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct: 908.947.1624 sstephen...@lifecell.com www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Sunday, May 27, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Hi, Any chance it is from your reservoir? Sometimes the paraffin is not filtered very well and there is some junk that collects in the chamber. (Yes I'm looking at you Paraplast!) Also is the reservoir you keep your cassettes in prior to embedding clean? Perhaps junk on your tampers? Amos On Sat, May 26, 2012 at 1:00 PM, wrote: > Message: 1 > Date: Fri, 25 May 2012 13:02:29 -0400 (EDT) > From: Ann Specian > Subject: [Histonet] Embedding > To: histonet@lists.utsouthwestern.edu > Message-ID: <8cf08af4cf811bc-c74-9...@webmail-m025.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > We are having a problem with floaters in our blocks which occur during > embedding. We have multiple forceps which are placed in heated wells and > each cassette is embedded with a new forcep. We also wipe with a gauze, > but we are still getting floaters embedded in the cassette from time to > time. > > Does anyone do anything else to prevent this? > Thank you, Ann > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassett Labeler
We use PrintMate by Thermo Fisher. Kinda loud by its pretty neat. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, May 08, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassett Labeler We have to purchase a cassette labeler ASAP, Any suggestions?? Appreciate any help? Behnaz Sohrab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how often do you change your hematoxylin?
Hi Meryl, What stain are you using your Hematoxylin for? We use Harris Hematoxylin for H&E staining and we filter it each day that we need to use it.We change it once per month. We are also not a high volume lab. We are a histology department in a biotech company. Your suspicions are right that it could be getting diluted by water dripping off the slides if they are not properly drained after the water rinsing step. Sheryl. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meryl Roberts Sent: Sunday, May 06, 2012 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
