[Histonet] Looking for suggestions, ideas

2014-08-26 Thread Thomas Jasper
Hi Folks,

Here is our situation.  In the not too distant future we will be receiving 
additional specimens from 3 dermatologists.  What they need is to have these 
specimens processed, embedded, cut and H/E stained.  Once we've got the slides 
coverslipped they are to be sent back the dermatologists.  We are not doing the 
interpretation.  What I'm looking for is an idea about getting these into our 
LIS (PowerPath) and designating them for return to this group.  We should be 
able to capture the TC and need to account for the workload.

I'm thinking we could prefix them differently at the time of accessioning and 
then create something in the LIS attached to that designation which only 
generates a TC.  I'm wondering if anyone else does anything like this?  I'm 
also interested in having this differing designation for efficient workflow.  I 
don't want any of these making their way to our pathologists.  I've thought of 
identification by color of block and a new prefix.  Just not sure if/what 
others are doing, how difficult to implement for an LIS standpoint (separation 
of TC and PC) and any other considerations.

Thanks, I know I've got all the superior minds out there.  Your thoughts are 
greatly appreciated.

Tom Jasper


Thomas Jasper HT (ASCP) BAS
AP/CP Supervisor
Deaconess Hospital
600 Mary Street
Evansville, IN 47747
thomas.jas...@deaconess.com
812-450-2485


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RE: [Histonet] Blade Rationing Follow-up

2013-06-19 Thread Thomas Jasper
You could start with cost comparison of blades (since this whole thing started 
as a blade issue).  Reps are willing to let you demo some before committing and 
you might save money if everyone likes a less expensive blade.  You can do the 
same for paraffin and slides as well as reagent alcohols and xylene.  Again, 
these are things the manager should be getting after.  Does your lab adhere to 
Lean principles?  There may be some cost savings there if you can accomplish 
tasks more efficiently; get the work done in less time or cutting back on 
overtime.  It's a bit difficult to say as much depends on the nature and scope 
of your service.  All situations are unique - do you send a lot of work out?  
Could you take it on in-house?  Or are you trying to do things in-house that 
could be sent out and have a cost positive effect?  Lots of questions for the 
manager...

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: Tuesday, June 18, 2013 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blade Rationing Follow-up

I really appreciate everyone's constructive comments regarding my post on blade 
rationing.  Lots of you said there are many other ways to cut costs in the lab. 
 I would like to hear some of your suggestions so I can take them back to my 
manager.  I'd like to give her some legitimate alternatives to her proposal. 
Would like to contribute to solving the problem of cutting costs.

Thanks again



Teresa Moore, HT
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FW: [Histonet] Blade Rationing

2013-06-18 Thread Thomas Jasper


-Original Message-
From: Thomas Jasper
Sent: Tuesday, June 18, 2013 8:52 AM
To: 'Teresa Moore'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blade Rationing

Teresa,

I concur with the all the responses.  It seems your lab manager is not grasping 
the technical reality you must work under.  If you are being asked to save 
money on blades why not try some different brands or negotiate some better 
pricing?  That is something the lab manager can work on.  Also, I would think 
you are doing your best optimize the use of each blade.  You should be able to 
get 3 good cutting areas per blade before they're spent.  Another consideration 
is having some blades for facing in only.
I'm guessing the manager is being pressured to cut cost.  I would look in other 
areas and at other items.  Blades are of too critical importance to mess around 
with much.
Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
AP Supervisor
Deaconess Hospital
Evansville, IN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: Monday, June 17, 2013 4:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blade Rationing

I work in a hospital, there are three of us on this particular shift and we cut 
approx. 200 blocks, give or take a few.  Our histo lab manager is telling us we 
should only be using one pack of blades (50 per pack) a month.  I'm wondering 
what other techs think of this especially lab managers and supervisors.

tmoor...@gmail.com
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RE: [Histonet] Blade Rationing

2013-06-18 Thread Thomas Jasper
Teresa,

I concur with the all the responses.  It seems your lab manager is not grasping 
the technical reality you must work under.  If you are being asked to save 
money on blades why not try some different brands or negotiate some better 
pricing?  That is something the lab manager can work on.  Also, I would think 
you are doing your best optimize the use of each blade.  You should be able to 
get 3 good cutting areas per blade before they're spent.  Another consideration 
is having some blades for facing in only.
I'm guessing the manager is being pressured to cut cost.  I would look in other 
areas and at other items.  Blades are of too critical importance to mess around 
with much.
Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
AP Supervisor
Deaconess Hospital
Evansville, IN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: Monday, June 17, 2013 4:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blade Rationing

I work in a hospital, there are three of us on this particular shift and we cut 
approx. 200 blocks, give or take a few.  Our histo lab manager is telling us we 
should only be using one pack of blades (50 per pack) a month.  I'm wondering 
what other techs think of this especially lab managers and supervisors.

tmoor...@gmail.com
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[Histonet] Unsubscribe

2013-05-21 Thread Thomas Jasper
 

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[Histonet] FW: Automated Microtome question

2013-02-12 Thread Thomas Jasper
See below.
Thanks,
tj


From: Thomas Jasper
Sent: Tuesday, February 12, 2013 8:56 AM
To: 'histonet-requ...@lists.utsouthwestern.edu'
Subject: Automated Microtome question

Hi Everyone,

Question - Does anyone know if there is a regulation in place (OSHA, i.e.) 
about having automated microtome(s) available to histologists if requested?  In 
other words; I was under the impression that after 2005 (I think...) if someone 
wanted an automated microtome the institution they work at is mandated to 
provide it to them.

This is not to say that folks must use an automated microtome; just that it's 
there or would be obtained for them.  I thought I'd heard something about this 
a few years ago.  With the repetitive motion issues today I was thinking this 
was a regulation of some sort.

Thanks in advance for the input.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
AP/CP Supervisor
Deaconess Hospital
Evansville, IN

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RE: [Histonet] certification of histotechnologists

2012-05-25 Thread Thomas Jasper
Janet and Richard,

To answer your question...to my knowledge this has not been given
consideration in the U.S.(I could be wrong).  Perhaps you could explain
this concept a little bit.  I personally have a hard time understanding
how an AP discipline (histology) is taught, mastered well enough and
certified alongside clinical lab disciplines (microbiology, hematology,
blood banking and general lab).  To me this seems a daunting task.  Am I
to understand that in Canada and the UK someone certified in your
medical laboratory programs is able to bounce around and work in the
clinical labs and then be expected to show up in histology and work at a
competent level there as well?

I worked in a lab were an MLT came and helped us out now and then
because she knew a little about histology.  She did not cut sections too
well and one day severely filleted her finger.  Also, in a previous
supervisory position I was asked by the general lab for help in flow
cytometry from my tech specialist.  He spent time down there helping out
and worked in my lab as well (IHC specialist).  His mind was about fried
after 6 months or so and I felt it was an unfair and unrealistic
expectation for him to perform at a high level in both areas.

Maybe I'm not understanding this correctly.  I believe the standard view
in the US is that Histology and Cytology are close relatives in the
world of anatomic path.  The other medical lab disciplines,
microbiology, hematology, BB, etc., have people floating around that
understand the instrumentation and objectives of those areas.  Seems
it's fairly common for histologists here to assist and do testing for
cytology (a bit of heme as well).  Again maybe I just don't get the
concept.  Especially as our discipline develops and we find ourselves
moving into the world of molecular pathology; not to mention the other
areas of histology outside of human clinical applications.

Thanks,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology
Bend, Oregon 97701

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards,
Richard E.
Sent: Friday, May 25, 2012 5:36 AM
To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] certification of histotechnologists

Same in UK, I have never understood why  the separation in the  Land of
the Free.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet
Keeping
Sent: 25 May 2012 12:38
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] certification of histotechnologists

Just curious if  any consideration has been given to including
Histotechnology in your medical laboratory programs as we do in Canada?
our
graduates are certified for 5 different careers and shortages in one
particular laboratory does not seem to be a problem.
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RE: [Histonet] Unregistered techs

2012-05-24 Thread Thomas Jasper
Holy buckets, that's a shot below the belt!  Must say, I'm quite
surprised to see a comment like that from someone with 19 years
experience.  By the by, I understand the registered HT thing, what is a
"Florida licensed HTL"?  Is that something new?  But I digress.

I can see your point about the scam/bleed money thing, but that's
another discussion.  You think monkeys can be trained to do
histology...well, you're entitled to your opinion.  However, the
validity of an opinion depends on its basis.  In my opinion monkeys
cannot be trained to do our job.  I'm quite certain that everything I
did in the lab today (before returning to my office, reading this post
and writing my response) would be challenging for a lot of
folks...pathologists included...let alone a smart monkey.

I'm a bit confused seeing the name Scott Lyons in the post below, so I
don't want to direct my response to the wrong person.  If this is indeed
you, Jay, I've read many of your posts in the past.  In consideration of
that, I'm thinking maybe you're exaggerating to make a point, maybe
having/had a bad day or both.  I agree there is no substitute for
experience.  And I agree that many people with advanced degrees can be
all thumbs in a lab, or maybe have a hard time transitioning book
learning into hands on action.  Come to think of it monkeys are pretty
dexterous...so maybe we're taking this all wrong.

I'm not responding to "light someone up" or get into a war or words
with.  I'll just say that I hold those of us doing this work in high
regard, monkey or not.  And that includes you too Jay...I've not met you
personally, but honestly you're no monkey.

Regards,

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay
Lundgren
Sent: Thursday, May 24, 2012 2:02 PM
To: Kim Tournear
Cc: histonet
Subject: Re: [Histonet] Unregistered techs

Scott Lyons sln...@yahoo.com

 Give me a break, HTs and HTLs do not make diagnoses or treat patients.
I
am a registered HT and a Florida licensed HTL with 19 years experience,
I've done it all in the lab. I believe the certification and licensure
of
techs is a scam to bleed more money from people. Honestly, you can train
a
monkey to do our job. And I don't want to hear from everyone saying it's
an
art form, we are just as much needed as pathologists, blah, blah,
blah... I work where they are hiring people from a masters degree
program for histology with certification, THEY KNOW NOTHING. Experience
it
where it's at, whether certified or not, get off your high horse.






















> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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[Histonet] Pro-Par

2012-02-07 Thread Thomas Jasper
Hi Folks,

 

Just curious...anyone using Pro-Par care to share an opinion?  Have
considered it off and on over the years and now wondering what folks
think.  Also, anyone using it with tape coverslippers?  Maybe this has
been asked and I wasn't keeping up with the thread.  

 

Thanks,

Tom Jasper

 

Thomas Jasper HT (ASCP) BAS

Histology Supervisor

Central Oregon Regional Pathology Services

Bend, Oregon 97701

541/693-2677

tjas...@copc.net

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RE: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested

2012-01-28 Thread Thomas Jasper
Ray,

Took the time to read your post.  You make excellent points.  Getting at the 
gist of your "wannabee" comments.  What boggles my mind is - how or why someone 
would try to pull something off like that.  Sooner or later (hopefully 
sooner...like before actually hiring them) the charade will be discovered.  
Misrepresenting oneself and false or misleading information given on an 
application is generally grounds for dismissal.

Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can".  In the 
end you are right about finding ways to determine if an applicant is "legit".  
I've come to believe that in the Histology world - if you meet or hear of 
someone you don't know...someone you do know...knows them.  At least that seems 
to be true almost all the time.

Kind regards,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
koelli...@comcast.net
Sent: Saturday, January 28, 2012 10:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] interview cutting-OT-disarmingly long for 
deletiondisinterested








Or as Gayle wisely pointed out it might be interview sectioning to 
differentiate those who "cut out" on an interview. 


While there is no right or wrong to this question, I'm still not convinced that 
it is a useful tool for you or HR to just have a routine "can cut (section) on 
rotary microtome" check box on application the same as you do for a "current 
address" or "reference contact" check box on a form. As I pointed out in my 
original stupid reply, willfully breaking my own internal rule to avoid taking 
up these gray (not black and white scientific) discussions, it would depend on 
the circumstance (unknown person from unknown parts vs. someone from part of 
the "histology community" well known). If I call "x" who I've known for years 
about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" 
worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who 
sectioned (#) blocks a day. Devastated to see him/her go but know they had to 
move along with husband/wife. Great cutter and everyone liked him/her". Having 
him/her sit down to now cut 10 blocks to see "if they can cut" as a routine 
question accomplishes WHAT?" If someone mysterious with no background walked 
in, sure have them cut although there have been numerous fantastic options 
already posted how to weed them out prior to sectioning a finger off. A 
(purposely) mis-processed block with tissue now shrunken in from block face and 
a question of "we need a recut, what would you do for this block" will let you 
know in about 2 seconds whether or not this is a histotech impostor. Or looking 
at a blandly stained, necrotic section under microscope and asking "interpret 
this section" will tell you something of who or what this person is. 
Personally, I'd far rather have a person who is energetic, scientifically and 
intellectually confident and talented, personable, works well within the 
"symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new 
environment set-up than a (female or male) diva who cuts 10 perfect blocks but 
who has that nearly imperceptible tint of not a complete team player or dubious 
personality. A routine check box "can cut" I think is just a waste of time and 
resources unless a particular circumstance warrants it. 


Someone asked "would you hire a secretary without a wpm typing test". 
Absolutely, beyond any doubt. If the transcriptionist next door wants a 
secretary position and routinely types 3 times faster than is required as a 
secretary; why a wpm test? If I call someone I know across state where this 
applicant worked for last 10 years and "she's an immaculate and fast typist 
beyond anything we've ever had and so sorry she had to move", I'd rather then 
concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 
years ago and has been a house-husband or house-wife for 25 years and starting 
back now or if someone walks in off the street to apply then beyond any doubt; 
they take a typing test. 


Someone pointed out that all musicians play their instrument in application to 
test for the orchestra. Of course but for a completely different reason. You 
could give an "oral test" to 1,000 musicians of which 999 would know how to 
transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify 
the composer if listening to an excerpt from the Overture-Midsummers Night 
Dream. That's not what the interviewee is l

RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin

2011-08-30 Thread Thomas Jasper
Matthew,

I am in agreement with you. I appreciate Peggy's comments and understand the 
wherefore and the why regarding this.  Unfortunately, something has been lost.  
We also serve as a clinical internship site for students coming out of Clover 
Park in the Seattle area.  This is a 9 week stint and a challenge considering 
all we'd like to expose the student to in such a short time.  As you mention in 
your post: speed, accuracy and quality are paramount and we do our best to 
prepare folks for the real world.

I too, am glad to have taken the practical.  Again, it's unfortunate that the 
practical isn't practical anymore.

Thanks,

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta
Sent: Tuesday, August 30, 2011 10:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin

Hey all,

I found Peggy's comments on why the practical was discontinued to be
very interesting. Of late I have had some experience with a new HT that
graduated from a program and passed the current HT exam. 
So, as they say in Great Britain, to stick a pin in the ASCP reasons.

This new fresh and shiny HT has all the book knowledge we needed them to
have. What they did not have was any technical skills.
1) never used a microscope or centrifuge.
2) no special staining experience
3) no embedding experience
4) no cutting experience

When they cut or embed they are no were near the speed, accuracy or
quality that is needed in our industry. While they can answer any
question you ask them they just do not have the technical skills one
would expect from a new graduate.

I have learned several lessons from this experience.

1) I am so very glad I was one of the last HT's to have taken the
practical
2) Any new HT's will be taking a practical if I am involved in the
selection process.
3) I will question they quality of any new HT from this particular
program

While I am sure that there are many new HT's that do have the skills
needed, this one experience has caused me to be more cautious.

Respectfully,

Matt Lunetta 
BS, HT (ASCP)




Message: 2 
Date: Tue, 30 Aug 2011 18:09:46 +0200 
From: "Gudrun Lang" 
Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical 
To: "'Bob Richmond'" 
Cc: histonet@lists.utsouthwestern.edu 
Message-ID: <8b7976b131854abc8db236fab5026...@dielangs.at> 
Content-Type: text/plain; charset="iso-8859-1" 

Dear Dr. Richmond 
Here in Austria we have a job open for a pathologist with 5 years 
experience. ;) 
Please, think it over to come. Lovely mountains, lovely techs... 

It sounds, like you are from that sort of pathologist techs dream of. 
Gudrun 


-Ursprüngliche Nachricht- 
Von: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Bob 
Richmond 
Gesendet: Dienstag, 30. August 2011 04:43 
An: histonet@lists.utsouthwestern.edu 
Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical 

I really appreciate Peggy Wenk's analysis of the practical examination 
and why it had to be dropped. I never really understood the issue 
before. 

I must confess I always enjoyed helping the prospective examinee 
obtain exactly the right tissue. 

"No, this endometrium is poorly preserved. We'll arrange with surgery 
for a completely fresh specimen - I'll block it initially for the 
diagnosis, then we'll fix it overnight and then block it exactly to 
specifications. - Ick - this one's been curetted - we'll get another 
one". 

"I'll block the margins of this colon resection specimen, then we'll 
pin a portion of tissue onto paraffin and fix it flat overnight." 

"Next time I do an autopsy we'll get a lumbar spinal cord in the 
intact dura. I'll open the dura dorsally and ventrally with iridectomy 
scissors, then we'll hang it in neutral buffered formalin for two 
days. Then I'll tie the dura and dependent nerves with a cotton 
string. When you embed you'll remove the string, taking care that dura 
and nerves remain in position. After that it's all yours. If it 
doesn't work the first time, we've got three more levels in the jar." 

OK, I'm a geek, I'm 72 years old, I got a right. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

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[Histonet] Sox 10

2011-07-27 Thread Thomas Jasper
General question -

 

Is anyone out there running Sox 10 on the Ventana Benchmark XT?  Any
dilutions or protocols would be appreciated.

Thanks,

Tom Jasper

Central Oregon Regional Path

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RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-28 Thread Thomas Jasper
Hi Stella,

I would be more than happy let you take this response to your lab
management.  First of all, just a bit of background - I have been a
histologist for over 26 years.  I have an Associate Degree in
Histotechnology and a Bachelor's Degree in Applied Science.  I am a
registered HT (1986) ASCP certified #12664.  I have worked in;
university and VA clinical settings, pharmaceutical research, major
medical and now independent clinical service.  I have been in
supervision for over 10 years and function as a working supervisor. I
have been responsible for Cytology, Autopsy and Transcription as well as
Histology.  I served for a number of years as a safety officer.  I have
significant experience with immunohistochemistry from manual kits, using
concentrated antibodies in multiple species application to running the
latest in automated IHC.

Having said that, the notion that a single histotech should be able to
cut 100 blocks an hour is sheer lunacy!  Expecting anyone to even
attempt such an unrealistic goal is dangerous, irresponsible and
ridiculous.  I seriously question "their own research".  I'm sure you
couldn't sell it in a deli as it sounds like nothing more than bad
baloney.  According to my calculations, that would be 1 block every 36
seconds...let that sink in.  You mention you are dealing with med techs.
These med techs apparently have no concept about the realities of
Histology.  I am going to assume this is the case and you (Stella)
obviously know better.  I will lay out the basic problems and hope you
are able to drive home the point.

~ Volume - 100 blocks per hour equals 1 block every 36 seconds...really?
Can you make change for a dollar in 36 seconds, find your car keys and
start your car?  Now do this over and over and over again, hour after
hour.  Even 50 blocks an hour is insane.

~ Variety - Histologists cut blocks from every part of the human body
(or animal or plant).  The specimens can be big or small, thick or thin,
hard or soft.  They can be dry and brittle, full of sutures and staples,
under-fixed and poorly processed.  When sectioning you are subject to
humidity, air currents, quality of the knife edge and specimen
orientation (and you just gave me a whole 36 seconds).

~ Quality - This is the number one consideration in my lab and any lab
worth its salt.  Quality is not achieved in one block every 36 seconds.
I just mentioned a list of variables and out of that a histologist has
to produce a microscopic work of art, one slide at a time, every time.
Any pathologist worth his or her salt will tell you that.  If you aren't
giving a good picture to that doctor, he or she is not going to be
happy.  You will want to figure in some additional time beyond 36
seconds for all the rework you're going to get.

~ Patient Care - Every histologist knows that a specimen/block/slide is
a patient.  That patient could be your mom, dad, sister, brother or some
other loved one and must be treated as such (regardless of who it is).
Trying to force histology work through at an impossible rate is
practicing bad medicine.  Is that how you would want your biopsy
handled?  If there is nothing more important than the patient, I think
the patient is worth more than 36 seconds.

~ Safety - Safety is easy to practice and easy to ignore.  What are we
dealing with here...extremely sharp blades for one.  The occasional
histologist may be known to skirt a safety rule now and then.  Don't get
your fingers too close to the blade.  With automated microtomes there
are new and exciting technical features to consider from a safety
perspective.  Regardless of the situation, speed factors into safety.
Existing stress factors combined with new ones for unrealistic speed is
an accident waiting to happen.  And there are other mental health
considerations from undue stress.

~ Special Testing - Not only are quality sections required for standard
hematoxylin and eosin staining.  Quality sections are required for
straight chemical, special staining, immunohistochemistry and other
special procedure applications labs may run.  For example, some
pre-treatments or other protocol steps involved in IHC may be a bit
harsh.  To rush and produce less than desirable sections for any of
these various procedures, due to unrealistic quotas is a bad idea.  Once
again all of the above apply to special testing.

Well Stella, I don't want to write a book and I'm sure I've left out
some valuable information.  I used to have unionized techs working for
me at my previous position.  I don't know if that's the case for you.  I
can guarantee the union steward would've had a field day with this one.
Also, this is the type of thing that OSHA loves to get wind of, along
with any state agency that regulates labor.  Please contact me if you'd
like to speak about this further.  Not to sound extreme, but there are
other jobs and nice, reasonable people to work for.

Kind regards,

RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-25 Thread Thomas Jasper
Well Joanne, someone on the Histonet probably has a documented average.
It is a difficult thing due to the amount of variables that exist and
the differences from lab to lab.  Again, in my humble opinion (and not
knowing anything about your workplace) to me, working in a mid-sized
clinical service and dealing with the variety of specimens common to a
lab such as ours...if someone is cutting 25-30 blocks per hour, the
sections are high quality and the errors are negligible (a subjective
statement) I would consider that more than acceptable.  Others may be of
a different opinion, however I would be surprised if someone would think
that one block per minute is reasonable and realistic (except for your
employers).  

I'm sorry to hear that it took you 10 months to secure employment at
your current "Roman galley" ship of a service.  There are jobs available
and not enough of us to go around.  I'd seriously consider breaking the
leg iron, abandoning ship and taking your chances in the wider world.
There are a lot of nice, sane people out there and I'm sure you could
find a place that would appreciate and treat you fairly.
tj

-Original Message-
From: Joanne [mailto:joanne0...@comcast.net] 
Sent: Saturday, June 25, 2011 6:31 PM
To: Thomas Jasper
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut
per

Tom,

Thank you for your response.   As it took me almost 10 months to secure
a 
position, I'm not likely to be able to jump ship.

Is there a documented average for a histotech's performance to be judged

against?

Again, thank you.

Joanne
----- Original Message - 
From: "Thomas Jasper" 
To: "Joanne" 
Cc: 
Sent: Saturday, June 25, 2011 9:01 PM
Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut
per


Joanne,

In my humble opinion and without knowing anything about where you work,
this expectation is ludicrous.  I have a hard time believing that
anyone, regardless of experience could attain the goal you've mentioned
(including answering phones and running instruments). There are a
multitude of reasons why this is absurd.  Suffice it to say, I would be
suspect of any work coming out of such an operation, not to mention
those in charge and the poor souls trying to meet this goal.

I seriously doubt you'll be able to have much say about things since you
are so new.  My advice...get out and find a job working for realistic
people.

Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Sent: Saturday, June 25, 2011 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How many tissues an histo tech is suppose to cut per



i've only been working 2 months.  although older, i am new as a
histotech (graduated in may 2010, found a job in april 2011).  seems
management is setting a goal of a block per minute as far as cutting
goes for me.  i have until october to attain this goal. this minute for
cutting is to include facing, writing out slides, cutting, and putting
tray into symphony stainer (not to mention getting up to answer the
phone, fielding questions regarding send-out cases, and other slight
"cutting interruptions).   this seems an extreme, possibly unattainable
goal.  i'm up for a challenge  at age 53, but any advice would be
SWONDERFUL :)

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RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-25 Thread Thomas Jasper
Joanne,

In my humble opinion and without knowing anything about where you work,
this expectation is ludicrous.  I have a hard time believing that
anyone, regardless of experience could attain the goal you've mentioned
(including answering phones and running instruments). There are a
multitude of reasons why this is absurd.  Suffice it to say, I would be
suspect of any work coming out of such an operation, not to mention
those in charge and the poor souls trying to meet this goal.

I seriously doubt you'll be able to have much say about things since you
are so new.  My advice...get out and find a job working for realistic
people.

Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Sent: Saturday, June 25, 2011 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How many tissues an histo tech is suppose to cut per


 
i've only been working 2 months.  although older, i am new as a
histotech (graduated in may 2010, found a job in april 2011).  seems
management is setting a goal of a block per minute as far as cutting
goes for me.  i have until october to attain this goal. this minute for
cutting is to include facing, writing out slides, cutting, and putting
tray into symphony stainer (not to mention getting up to answer the
phone, fielding questions regarding send-out cases, and other slight
"cutting interruptions).   this seems an extreme, possibly unattainable
goal.  i'm up for a challenge  at age 53, but any advice would be
SWONDERFUL :)   
 
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RE: [Histonet] IHC pos. & neg. control question

2011-05-19 Thread Thomas Jasper
Pete,

Can't argue with that.  I think for the sake of expediency most clinical
services run a "known" positive and a patient slide for negative.  In
the case of H. Pylori, for instance, we may cut a box of control slides
and it's possible to go through the area where the organisms were.  This
also happens with controls that demonstrate positivity by other means
epithelium, tumor, etc.  We may have to re-run tests in these
situations. I believe we are similar to many clinical labs in our
reliance on known positives.
tj

-Original Message-
From: pete.peder...@healthonecares.com
[mailto:pete.peder...@healthonecares.com] 
Sent: Thursday, May 19, 2011 2:54 PM
To: Thomas Jasper; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

Thomas,

Agreed, however, how can you say with certainty that the control is
still good, or the antibody is still performing optimally?
Hypothetically speaking, if you had a known positive control and ran it
like a patient specimen (positive and negative) and had staining in the
negatively stained control that you had been only running as a
positively stained control prior, how would you proceed? What good is a
positive control without if it is not treated identically as patient
tissue. If you had none specific staining in a patient negative but is
was also there in your known positive control which you stained
negatively as well, then you could mark up to nonspecific staining to
reagent or IHC user error. If the negatively stained positive control
stains truly negative and the patient negative has nonspecific staining
then you would know patient tissue is compromised or has been mistreated
somewhere along the way because your positively stained and negatively
stained positive controls demonstrate the staining was done correctly,
correct? 

Pete Pedersen   B.S. HTL (ASCP)
Anatomic Pathology Supervisor

-Original Message-
From: Thomas Jasper [mailto:tjas...@copc.net] 
Sent: Thursday, May 19, 2011 1:39 PM
To: Pedersen Pete; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

Pete,

When you run a positive control.  The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling.  It would be impossible for this not to be so.  However,
with a negative, the concern is seeing how the patient tissue turns out
when subjected to all the same conditions, minus the antibody.  
tj

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pete.peder...@healthonecares.com
Sent: Thursday, May 19, 2011 12:31 PM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

Glen,

If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn't that mean the same thing towards
the use of a positive control? How can you guarantee the positive
control tissue was treated the same as the positive stained patient
tissue? According to your logic it cannot. Therefore, without the use of
a negative control how can you say the staining seen in the positive
control is truly positive and not artifact? Best practice says use
positive and negative patient and control tissue. Please enlighten me if
you know anything to the contrary? 

Pete Pedersen   B.S. HTL (ASCP)
Anatomic Pathology Supervisor

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense.  Because it would not be from
the patient tissue being tested, how do you know if it was handled the
same as the patient tissue?  For example:

1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that
could cause non-specific staining.
5) Was the patient abducted by aliens?

My point is that running a piece of tissue as a negative control that is
not even from the patient being tested throws all of the conditions that
the patient tissue was exposed to prior to and during processing out the
window.  This makes NO sense.

Glen Dawson  BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject

RE: [Histonet] IHC pos. & neg. control question

2011-05-19 Thread Thomas Jasper
Pete,

When you run a positive control.  The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling.  It would be impossible for this not to be so.  However,
with a negative, the concern is seeing how the patient tissue turns out
when subjected to all the same conditions, minus the antibody.  
tj

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pete.peder...@healthonecares.com
Sent: Thursday, May 19, 2011 12:31 PM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

Glen,

If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn't that mean the same thing towards
the use of a positive control? How can you guarantee the positive
control tissue was treated the same as the positive stained patient
tissue? According to your logic it cannot. Therefore, without the use of
a negative control how can you say the staining seen in the positive
control is truly positive and not artifact? Best practice says use
positive and negative patient and control tissue. Please enlighten me if
you know anything to the contrary? 

Pete Pedersen   B.S. HTL (ASCP)
Anatomic Pathology Supervisor

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense.  Because it would not be from
the patient tissue being tested, how do you know if it was handled the
same as the patient tissue?  For example:

1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that
could cause non-specific staining.
5) Was the patient abducted by aliens?

My point is that running a piece of tissue as a negative control that is
not even from the patient being tested throws all of the conditions that
the patient tissue was exposed to prior to and during processing out the
window.  This makes NO sense.

Glen Dawson  BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question

I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.



Email:

"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control.  The patient's own tissue cannot be used as a
negative
control.  The tissue that stained positively must serve as the negative
control without the antibody.  This is critical and you need to correct
that
immediately."





Curt



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RE: [Histonet] RE: Procedure for making Gram Control

2011-03-17 Thread Thomas Jasper
Some sort of small, snack sausage of questionable quality.  I'm not familiar 
with anything like that in Europe, but maybe you could determine that somehow.
Good Luck!
Tom Jasper
Bend, Oregon


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J.
Sent: Thursday, March 17, 2011 1:47 AM
To: Walter Benton; Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Procedure for making Gram Control

What would be the equivalent of a Slim Jim in Europe? The Netherlands to be 
more precise?
 
 



Van: histonet-boun...@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] RE: Procedure for making Gram Control



Making you own...not sure how to do that, but Slim Jim's work in a pinch.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi 
(HorizonNB) [randi.ha...@horizonnb.ca]
Sent: Wednesday, March 16, 2011 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Procedure for making Gram Control

Hello out there in Histoland,

I'm looking for a procedure for making your own Gram controls.  Any
assistance would be appreciated.

Thanks, Randi
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[Histonet] California Histology Society

2011-03-15 Thread Thomas Jasper
Hello Histofolks,

 

Am wondering if someone from the California Society would be kind enough
to contact me regarding the state convention this year?  I'd like to
send one of my staff.  I was under the impression that it commences
sometime in May.  I've checked the website and haven't found any
information on this.  My contact information is below.

 

Thank you kindly,

Tom Jasper

 

Thomas Jasper HT (ASCP) BAS

Histology Supervisor

Central Oregon Regional Pathology Services

Bend, Oregon 97701

541/693-2677

tjas...@copc.net

 

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RE: [Histonet] High Complexity Testing

2011-02-08 Thread Thomas Jasper
Completely agree with you Glen.  However, do take exception to the initial 
comment on this thread about "a machine does all the work".  The "machine does 
all the work" comment is very telling about what this pathologist (or group of 
pathologists) knows and does not know about running IHC.  If the "machine does 
all the work" what do you need the IHC staff for?  To me, that's like saying 
when a pathologist using image analysis, that the "machine does all the work".  
Granted you're going to have people with various skill sets and knowledge of 
histochemical processes, but comments (and attitudes) like that are degrading 
in nature.  If the "machine does all the work" why doesn't this pathologist go 
to the nearest McDonald's and recruit from they're application files?  Just 
trying to make a point here and tip my hat to everyone working diligently and 
intelligently even though you may have a "machine doing all the work".

Tom Jasper

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Tuesday, February 08, 2011 10:40 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] High Complexity Testing

Alright, if IHC is not high complexity testing, CAP should cut that massive 
part of their inspection in half and concentrate more on the pathologists' 
ability to accurately interpret the staining.  Too much CAP regulation, 
Proficiency Testing & validation requirements involved if all IHC is is part of 
"Processing".

My Opinion,

Glen A. Dawson
Milwaukee, WI

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Tuesday, February 08, 2011 12:17 PM
To: Whitaker, Bonnie
Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] High Complexity Testing

I should not have included CLIA in my e-mail as it would seem it has clouded
things a little.  I do apologize.  Initially when these issues and
guidelines came about CLIA and CAP dovetailed as far as Histology  was
concerned.

Shelia you were looking for contacts that would help you with getting a more
solid base to meet these regulations.  If you go to the CAP website and
click on the IHC link you will find links and publications to assist you.  I
would recommend that you contact the Applied Immunohistochemistry society as
well.  NSH or your state/regional society may also have additional
information.

Should I see something else in my searches I will most willingly forwarded
them to you.

Vikki

On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie  wrote:

> Hi All,
>
> There is a difference in performing a task (immunostaining) that is
> complex, and performing "high complexity testing" as the CLIA regulations
> govern.
>
> Yes, staining is a complex task, and it requires knowlegable techs to
> ensure that it is properly done, and to troubleshoot  difficulties when
> necessary.
>
> It is "high complexity testing" because "testing personnel" in anatomic
> pathology are pathologists (and the non-physician people performing gross
> examinations, who must meet "high complexity testing personnel"
> requirements.
>
> "Testing personnel" as defined by CLIA, are the people that report results
> of that test, not people who perform other related duties.
>
> That's my explanation of the whole mess.
>
> Bonnie Whitaker
> AP Operations Director
> Ohio State University Medical Center
> Department of Pathology
> 614.293.5048
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
> Sent: Tuesday, February 08, 2011 12:22 PM
> To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu;
> Sheila Fonner
> Subject: RE: [Histonet] High Complexity Testing
>
> I must disagree with this assessment of what makes a test complex.  If the
> test is done properly [the responsibility of the technologist] then the
> reading to the test is a visual determination that requires experience on
> the part of the pathologist, but if the test is not done properly, will the
> pathologist be able to tell the technologist what to do to fix the problem?
>
> Where's the Tylenol?
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
> Sent: Tuesday, February 08, 2011 9:58 AM
> To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner
> Subject: RE: [Histonet] High Complexity Testing
>
> While the test is high complexity it is the READING of the test by the
> pathologist that determines its complexity.  Because histotechs do not
> report the results our part of this test is not high complexity.
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
> 1 Child

RE: [Histonet] Stupid, stupid static!!

2011-01-05 Thread Thomas Jasper
Hi Sarah,

One of our staff uses the static spray now and then.  It works pretty
well but the "fragrance" isn't the greatest.  We also have a couple of
humdifiers in the lab.  These seem to help as our air in central Oregon
is a bit dry on this side of the Cascades.  Don't know if dry air is an
issue for you in Austin, but we love our humidifiers here.  Actually one
is this cool blue penguin that shoots mist out of his beak...makes me
smile every day.

Later,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor 
Central Oregon Regional Pathology Services
Bend, Oregon 97701
tjas...@copc.net
541/617-2831

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, January 05, 2011 11:15 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stupid, stupid static!!

So where the microtome is here that I have to use we have to wear those
blue hospital booties and disposable lab coats (the white paper type
ones).  With me and several other people walking around in those booties
the amount of static electricity is to say the least frusterating!!
Does anyone know of anything I can do to get rid of the static?

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

 

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RE: [Histonet] Locking up formalin

2010-10-15 Thread Thomas Jasper
Hi Victoria,

I've never heard of this, of course it could exist somewhere.  I wonder
what would prompt this.  There are obvious safety issues to consider.
Perhaps an unauthorized party, somewhere, got into some formalin and
caused problems?

Locked up or not, proper spill containment is mandated by OSHA (I
believe) so, again perhaps an unauthorized personnel issue?  

I've worked in the Upper Midwest and Pacific Northwest and have not
heard of this.  I regularly attend the NSH and have not heard anything
at the meetings either.

Thomas Jasper
Histology Supervisor
Central Oregon Regional Path

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Spoon,
Victoria
Sent: Thursday, October 14, 2010 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Locking up formalin

Is anyone aware of regulations stating that formalin has to be locked
up-  put in locked cabinets when not under direct supervision?

Applying to either clinics where specimens are collected into formalin
containers or in the pathology lab?

Thank you


Victoria Spoon
Anatomic Pathology Manager
Bassett Medical Center
Cooperstown NY 13326
victoria.sp...@bassett.org
Tel(607) 547-6357
Fax(607) 547-3203



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RE: [Histonet] RE: Cutting, Processing, etc

2010-09-17 Thread Thomas Jasper
Hi James,

I would take it a step farther with the continuing ed.  I think it's beyond the 
supervisors it gets up into lab administration (clinical lab world).  I 
personally know of a group of great folks that work hard and run a quality 
service.  In the last 3 years they've had a major drop off in their continuing 
ed.  And it, of course, is tied to the budget.

Unfortunately, in this case (my view) those making the money decisions are 
missing the value.  It seems they're unwilling to make the investment.  I fear 
that in 5 years or less (if it continues) this service will suffer.  I suspect 
there are other folks out there in the same boat.  My hat is off to everyone 
out there working hard in our field and to the "enlightened" administrators and 
physicians that advocate continuing ed.

Have a great weekend.
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of james leroux
Sent: Friday, September 17, 2010 10:55 AM
To: 'Nails, Felton'; histot...@imagesbyhopper.com; 'mohamed abd el razik'; 
Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Cutting, Processing, etc

Felton,

  I would have to disagree with your assessment of the emails.  Our field is 
very strong and is not in decline.  Unfortunately, some "supervisors" around 
the country are relying on archaic methods and do not want to see or welcome 
change within the histo lab. We call ourselves professionals and yet not all of 
us are required to do continuing education?  I read emails everyday and laugh 
at some of the bloviating that goes on inside this forum.  I am glad the 
questions are asked, but I am also amazed at some of the responses that are 
shared with everyone.  I choose to respond one on one with the person asking 
the question.  Basic histology deals with didactics and this particular inquiry 
dealt more with OJT.  There are many ways to get the same job done; are there 
more efficient ways?  Probably, but this does not mean we all do our job the 
same way.  I am not concerned about the future of Histotechnology. I embrace 
the opportunity to teach the young technicians about a field that sees a change 
almost daily.  I am not here to offend either, but rather to defend an 
occupation that is as fascinating as it is frustrating.

Respectfully,
 

James Leroux, AAS, BA, HTL
Histology Supervisor
Petroglyph Pathology
640 Quantum Rd. 
Rio Rancho, NM 87124
(505) 924-0219

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Friday, September 17, 2010 11:03 AM
To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; 
Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Cutting, Processing, etc

As I look through and monitor questions, it is apparent that our field is 
declining. These are very basic questions not about special stains or IHC 
stains but basic histology that should have been taught in histology 101. My 
fear is that as we get older and leave the field, who and what will be left to 
carry the torch. Those of you who ask, don't take offense to my thoughts but 
take action and pick up a book and read. You will improve yourself and the 
field.
Just my thoughts, if I offended you it was not my intent. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Friday, September 17, 2010 11:42 AM
To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Cutting, Processing, etc

My first reaction to the "what is happening to our field", was WOW.  It seemed 
unkind to me, as if they original poster should not have asked these questions. 
 With further reading of the replies to this post, I am not so sure it was an 
unkind response, but one of potential shock and dismay to the idea that labs  
might not be producing the quality work that most of us employ on a daily basis.

Amy, in answer to your questions, I will echo some of the sentiments that I 
have read here.

1. Facing of blocks.  We use one blade to face blocks and another, new blade 
when we do our actual sectioning.  In my case, I face as many as I can, knowing 
I am going to toss that knife when I am done facing.

2.  Soaking of blocks.  After facing my blocks, I will put them on a cold, 
damp, ice cube tray.  This will achieve two purposes for me, a) to chill the 
block and b) to introduce moisture into the faced tissue.  If I get a block 
that is particularly dry or hard (some calcified tissues for example), I will 
face them, put them face down on my water

RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions?

2010-07-30 Thread Thomas Jasper
Hi Tanisha,

I think the question might be...who's requirements are you referring to.
I see by the header you reference NY state.  I see by your signature
that you are in Indiana.  I am not aware of any CAP reg referencing this
and, to my knowledge most labs have their own job descriptions written
with their own qualifications listed.  You say "...right now I'm being
told..." so again, by who (whom).

You also reference a BA, I'm assuming this means BS or BAS works as
well.  I personally know very good supervisors that are associate
degreed and hold HTs.  I also know supervisors that have bachelor's
degrees and I wouldn't necessarily say that they are any better because
of it, however I think that's a "truism" across a lot of occupations.

Just trying to understand your question.

Have a good day.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neely,
Tanisha
Sent: Friday, July 30, 2010 7:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Lab Supervisor Requirements - New York
State/CAP Regula tions?

Hi Histonetters:

Our HR/QA team is struggling to understand exactly what the requirements
are for Histology Technicians/Technologists to become laboratory
supervisor. It is my understanding that the requirements are not exactly
the same as those for the traditional clinical lab. And until recently,
the regulations were vague regarding our field. 

Right now, I am being told that to be supervisor requires a BA degree
and 6 years of experience subsequent to receiving that degree. I am not
sure that is accurate. 

If anyone has any information they can share, I would greatly appreciate
it.

Thanks,

Tanisha N. Neely, HT (ASCP)
Global Histology Technical Liaison
Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214
<http://www.covance.com/> 


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RE: [Histonet] New CAP question ANP.22760

2010-06-26 Thread Thomas Jasper
c Specificity
>Accuracy on tests of known positive and negative controls
>Controls of known concentration
>Determine what could "Interfere" to confound the result
>
>
>Diagnostic Specificity
>Ability of a test to determine true diagnostic negative verses
falsepositives (Higher % FP = less specific)
>Requires comparison to a previously validated antibody
>
>
>IHC Specificity
>Ability of an antibody to bind exclusively to its particular
antigenin the absence of staining of other molecules
>Or, staining of other structures in addition to target  
>structures/cells
>
>(Sensitivity and Specificity adapted from: Theoretical and Practical 
>Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005)
>
>Tim Morken
>Supervisor, Histology / IPOX
>UCSF Medical Center
>San Francisco, CA
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu 
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
>jmye...@aol.com
>Sent: Tuesday, June 22, 2010 6:51 PM
>To: tjas...@copc.net
>Cc: histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] New CAP question ANP.22760
>
>Tom:
>
>As much as I agree with your acknowledgment that its seems a bit odd 
>for the CAP to have a blood-banker responding to AP-related issue, I'm 
>actually not surprised.  The folks in the 'clinical' lab have been 
>performing more comprehensive and complex validation procedures for a 
>very long time, and they wonder why IHC isn't expected to follow the 
>same requirements as chemistry, immunology, etc. -- IHC is, after all, 
>an awful lot like ELISA.  And rightfully so, because IHC is, under CLIA

>(which supersedes CAP), considered highly-complex, non-waived testing 
>-- and is, therefore, subject to the same Quality Systems regulations 
>(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing
performed in other areas of the lab.
>
>Could it be that, because AP produces qualitative results that are 
>interpreted by a pathologist and CP produces quantitative results that 
>are interpreted by an analyzer, we somehow think that CLIA rules don't 
>apply to IHC?  I certainly don't have the answer to that, but it make 
>me wonder what the future holds.  As witnessed by some of the newest 
>CAP 'standards' (including the question in question...no pun intended),

>e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens 
>must be tested, and where 10 of the positives must be weakly positive 
>-- an acknowledgment that validation specimens must be carefully 
>selected in order to obtain appropriate results), it certainly doesn't 
>appear that the regulation of IHC testing is going to become more
relaxed.
>
>Joe Myers, M.S., CT(ASCP)
>
>--
>
>Message: 12
>Date: Fri, 18 Jun 2010 12:38:07 -0700
>From: "Thomas Jasper" 
>Subject: RE: [Histonet] New CAP question ANP.22760
>To: "Mark Tarango" 
>Cc: _histo...@lists.utsouthwestern.edu_
>(mailto:histonet@lists.utsouthwestern.edu)
>
>Mark,
>
>Did you notice the credentials from this CAP representative? MT with a 
>Blood Bank specialty I believe.  What I glean from that is...more than 
>likely this person does not grasp the logistics of "contemporaneously"
>staining identical Abs from separate lots.  She also likely does not 
>understand the logistical application for detection and automation 
>either.
>
>I'm not trying to be overly critical of this person.  I'm sure she is 
>quite intelligent and would not have the MT/SBB if she wasn't 
>intelligent.  It comes down to a lack of understanding Anatomic 
>Pathology testing application re: automated IHC.  I believe this is a 
>common problem in and out of CAP. Many lab directors and other folks in

>positions of authority without AP/Histology/Cytology backgrounds seem 
>to believe that broad clinical lab modalities apply to Anatomic Path 
>scenarios.  I used to refer to this in my former position as - "Trying 
>to put the yoke of clinical lab onto anatomic path."  We are 
>laboratorians, but in many instances do not fit the general clinical 
>lab mold.
>
>It's unfortunate that CAP has put this person in the position to 
>respond.  It is apparent to me that she's not grasping the particulars 
>here.  She probably never will unless she decides to go into a working,

>automated IHC "tissue" lab and take the time to ask questions and 
>understand (learn) what we're all about.
>
>Thanks,
>Tom Jasper
>
>Thomas Jasper HT (ASCP) BAS
>Histology Supervisor
>Central Oregon Regional Pathology Se

RE: [Histonet] New CAP question ANP.22760

2010-06-18 Thread Thomas Jasper
Mark,

Did you notice the credentials from this CAP representative? MT with a
Blood Bank specialty I believe.  What I glean from that is...more than
likely this person does not grasp the logistics of "contemporaneously"
staining identical Abs from separate lots.  She also likely does not
understand the logistical application for detection and automation
either.

I'm not trying to be overly critical of this person.  I'm sure she is
quite intelligent and would not have the MT/SBB if she wasn't
intelligent.  It comes down to a lack of understanding Anatomic
Pathology testing application re: automated IHC.  I believe this is a
common problem in and out of CAP. Many lab directors and other folks in
positions of authority without AP/Histology/Cytology backgrounds seem to
believe that broad clinical lab modalities apply to Anatomic Path
scenarios.  I used to refer to this in my former position as - "Trying
to put the yoke of clinical lab onto anatomic path."  We are
laboratorians, but in many instances do not fit the general clinical lab
mold.

It's unfortunate that CAP has put this person in the position to
respond.  It is apparent to me that she's not grasping the particulars
here.  She probably never will unless she decides to go into a working,
automated IHC "tissue" lab and take the time to ask questions and
understand (learn) what we're all about.

Thanks,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark
Tarango
Sent: Friday, June 18, 2010 11:47 AM
To: McMahon, Loralee A
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] New CAP question ANP.22760

That's what I thought at first too.  It might be helpful to post this
letter that I got from the CAP about this.  I tried to argue with them,
but this is the answer I got.


Dear Mark,

Your questions were forwarded to me for response.



During the Audio-conference, the idea of comparing a previously stained
slide (that had used the "old" lot) to one stained with the new lot was
deemed acceptable, but not optimal. Doing a simultaneous staining using
old and new lots, better demonstrates the performance characteristics of
the reagent.  The reason parallel staining is considered best practice
is that all other variables, such as variations in the lot of detection
reagent or instrument function, are eliminated from consideration when
the slides are stained contemporaneously.



The antibody "getting weak over time" should not happen to a significant
degree if the antibody is used within its expiration date.  If the lab
is having this kind of trouble, it should look carefully at its storage
conditions.



Demonstrating acceptable performance of the new lot, before being place
into service, is *required* for all accredited laboratories.



To answer the last question, the key is to order the new reagent well
before you run out of the old lot so that the parallel stain can be
performed before the old lot is consumed. One multi-tissue slide control
slide would suffice to evaluate a primary antibody lot in most cases,
which helps to minimize the impact on the lab.



I hope that this information is helpful.  Thank you for your
participation in the Laboratory Accreditation Program.



Sincerely,



*Kathy Passarelli, MT(ASCP)SBB*

*Technical Specialist*

*Laboratory Accreditation Program*

*College** of American** Pathologists*

*Phone: 1-(800)-323-4040 ext 7486*

*e-mail:  **kpas...@cap.org* 



On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A <
loralee_mcma...@urmc.rochester.edu> wrote:

> I think that CAP means that you need to save the slide that you ran 
> from the previous lot and compare it to the slide that you have 
> stained with the new lot number.  To see if they are sufficient 
> diagnostic quality.  Not put both lot numbers on the machine at the
same time and then compare the
> slides?   We run Dako machines and it would be tricky to put both
numbers on
> the same machine.
>
> Although this is my interpretation.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
> 
> From: histonet-boun...@lists.utsouthwestern.edu [ 
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ 
> mpe...@grhs.net]
> Sent: Friday, June 18, 2010 12:41 PM
> To: Ellen Yee; Laurie Colbert
>  Cc: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] New CAP question ANP.22760
>
> I don't think I can do this with the automated system we are currently

> using. Ventana. Does any other Ventana users know if you can do this 
> in &qu

[Histonet] FW: test

2010-06-18 Thread Thomas Jasper
test

  _  

From: Thomas Jasper 
Sent: Friday, June 18, 2010 11:17 AM
To: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: test


Thanks, checking on connection.
tj
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FW: [Histonet] Are Histotechs considered "exempt" employees?

2010-05-11 Thread Thomas Jasper
 

-Original Message-
From: Thomas Jasper 
Sent: Tuesday, May 11, 2010 3:45 AM
To: 'Anthony Sandoval'
Cc: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

Anthony,

Don't know where you live in Cali or where you work.  But if you are an
HTL and have any decent skill/experience, I would think you are being
totally ripped off.  The cost of living in most parts of Cali alone
makes me wonder about this salary.  And when you ask about being
exempt...I'm assuming you are exempt?  If this is the case again a total
rip off.  I think you need to look for work elsewhere and check into
wages.  New students that are registry eligible start out much higher
than that to my knowledge.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
CORPS
Bend, Oregon 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony
Sandoval
Sent: Monday, May 10, 2010 9:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Are Histotechs considered "exempt" employees?

Hello fellow Histotechs, I have recently become certified as an HTL and
was wondering if anyone out there is an 'exempt' employee? I live in
California and feel that I am being taken advantage of.  I make 16.15$
per hour and frequently work 50 hour weeks. Am I off base? should I just
be grateful that I have a job, as my employer so frequently reminds me?
Thank you Histonet! 
you have been an invaluable resource in my career and assisting me in
passing the HTL! 


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RE: [Histonet] studying for ASCP certification exam

2010-04-14 Thread Thomas Jasper
Jennifer,

I think all the suggestions you've gotten so far are good.  I'd add -
The Theory and Practice of Histological Technique - by Bancroft and
Stevens.  I also believe there are NSH study materials available, maybe
the BOR guide you mentioned is the same thing, not sure.

Good luck!

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor 
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/617-2831
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: Tuesday, April 13, 2010 4:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] studying for ASCP certification exam

  I am in the process of studying for my HT certification exam and was
wondering if anyone had any recommendations on text books or study
guides they found to be helpful.  I am currently studying
"Histotechnology: A Self-Instructional Text", by Carson and just
recieved the "BOR study Guide for Histotechnology".  Are there any other
sources you would recommend?  I have taken a look at the suggested
reading list on the ASCP website but, there are quite a few books
listed.
 
Thanks in advance,
 
Jennifer Campbell
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RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Thomas Jasper
I'm inclined to agree with you Andrew.  Seems to me that CAP has become,
unintentionally (I'd like to believe) something of an unsavory, bullying
sort of entity.  I'm not certain about all the factors involved, but I
think a few things have definitely contributed to all of this CAP
negativity.

First of all - CAP was (is?) considered the accrediting "gold standard".
That's pretty heady stuff...possible ego inflation potential...high
horse attitude...elitism?  Not saying that was the plan, just an
unfortunate and unintended consequence.  I'm sensing a power trip here,
it's partly human nature (I guess) and it can definitely suck!

Secondly - as I recall, CAP got egg on their face a few years back in
the DC/Baltimore area I believe.  Someone can correct me if I'm wrong or
provide more specific details.  Anyway, the gist being - someplace
passed their CAP inspection and someone (an employee I believe)
contacted another regulatory agency because there was NO way this place
should've passed CAP.  When this all came to light CAP had to respond,
and as can often be the case, the response was overly compensatory.

I'm sure a lot of folks out there know what I mean.  Now CAP issues
confusing and sometimes unnecessary regulation changes and additions.
And of course we're all aware of the "super secret surprise"
inspections.  I'm not even sure of half the other hidden agendas and
possible ulterior motives.  Control issues, tarnished pride, bruised
egos and all conveniently cloaked in a drive for the best possible
patient care...who could argue with that standard?

During my previous employment, we "sweated" the details and worked
diligently to achieve our 1st CAP accreditation circa 2000.  I have to
admit, I did/do like the regulation format.  Having a question asked,
determining if it applies to your service, and then answering yes or no.
By taking it from there and doing things on the up and up, most any lab,
that's honest and conscientious should have the realistic expectation of
passing CAP.  That was then, it seems to longer be that way.  As I
mentioned, confusing language and reg. additions/changes, along with CAP
inspectors and their agendas have all been to the detriment of the
accreditation.

I was trained to look at CAP as "peer review".  In my experience, many
times this was not the case.  Many CAP inspecting "teams" wants to make
the "inspectees" (if that's a word) something of a clone, carbon copy or
version of the inspecting team's service.  This is another huge problem
and causes a lot of strife, hard feelings and red tape at and after the
summations.  The regulations, ideally, should be interpreted in the most
objective way possible.  Again, maybe it's human nature, but it seems
that people can't help being overly subjective re: interpretations of
any number of CAP regs.

I used to work with a pathologist that regularly attended the CAP
committee meetings.  At times I would bring issues to him I thought
relevant to CAP.  I don't recall the specifics but I do know they were
of a practical nature from a technical viewpoint.  I basically got the
brush-off and was led to believe that CAP wasn't interested in the
"technical" viewpoint and he wasn't going to bother with it.  This may
be a stretch in logic on my part...however, I can't help but think if
CAP would listen to technical folks as well as MDs, they'd be in a
better position right now.

I'm not inclined to throw the baby out with the bath water.  I think CAP
accrediting was established with good intentions. Somehow things have
gotten out of hand, and some have gone horribly wrong.  I think things
like QIP are good, although I've heard complaints about that as well.
I'm feeling lucky these days because CAP isn't in my life.  But my
attitudes and mind-set have most certainly been shaped by my CAP
experiences.  Please remember this is my opinion only, I am not perfect
and am only interested in practical application of sensible regulations
for optimal patient care.

Regards,
Tom Jasper
Histology Supv.
CORPS


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew
Burgeson
Sent: Thursday, April 01, 2010 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com

Sheesh is right, J.

CAP is all politics as far as I am concerned. It is all about protecting
the careers and paychecks of the general pathology community.

I am thouroughly unimpressed with JCAHO, CAP et al.

If all you need to legally run a laboratory is to be CLIA inspected,
then WHY BOTHER with these subjective entities?


The BS I have heard over the last few months concerning MOHS surgery
specimens is one glaring example of the limitations CAP has in
understanding fully certain nuances of the lab trade.

Ridiculous. Unless you want the marketing and potential "perception"
that you are better covered from a legal standpoint, CAP certs are
worthless. 

[Histonet] California Histology Society

2010-03-26 Thread Thomas Jasper
Hi There,
 
Anyone out in histoland know why there's no access to
www.californiahistology.org ?  I've tried a few times this morning with
no luck.  I want to send one of my techs to the symposium and need to
access the event site.  Thanks in advance for any help or explanation.
 
Tom Jasper
 
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
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RE: [Histonet] Contamination..processor?

2010-03-24 Thread Thomas Jasper
Hi Brandi,

Don't know if I can solve your problem...but here's a few questions.

1)You've determined that the floater (tonsil cells in this case) are in
the block.  Did you determine this because you consistently see the same
floater in the same spot, level after level, slide after slide?

2)Do you use a forceps warmer at your embedding station?  If so, have
the wells of that warmer been cleaned out lately?  It can be a source of
floaters.

3)Do you have 2 processors?  If so, are you running separate programs
(on separate machines of course) for large and small specimens?  If you
can do this and you are not, you might want to consider it.

4)While it's possible you could be picking something up from your
processor, I would not be initially suspect of it.  Are you running
clean runs after processing runs?  If you are this should basically take
care of any residual tissue floaters that may have gotten out of a
(tonsil) block, or any other block for that matter. 

You embedded the GI biopsies first, so I would not suspect the embedding
center work surfaces to be a source of your tonsil floater.  Some
machines have little grooves to allow waste paraffin to drain off,
sometimes things can be trapped there.  Also, you say that the
pathologist grossed the tonsils after the GI's.  I believe you and
him/her, but was anything else grossed before the GI's?  Some type of
lymphatic tissue?  I tend to look to the grossing bench 1st for the
source of floaters because once it's done there, it shows up everywhere
else.  Also, when techs cut and embed, they have no choice but to cut
and embed whatever they're given.  There is no way to determine if what
you might be looking at is a floater.  And more often than not, when
cutting and embedding you've inherited a floater as opposed to
introducing one.


Good luck, hope this helps.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
tigger...@aol.com
Sent: Wednesday, March 24, 2010 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Contamination..processor?



Hello everyone.

 Today we have a problem with contamination.  The pathologist notes
cells from tonsil specimens here and there on our GI biopsy slides.  The
cells are in the block.  I'm trying to ascertain the source of the
contamination.  
 The grossing pathologist grossed the tonsils AFTER all GI specimens
yesterday (not source of contaminant).  We (the techs) embedded all GI
specimens first, trimmed, cut, floated and stained ALL GI specimens
BEFORE the tonsils (not source of contaminant).  The only other source
of the contamination I can think of is from the tissue processor.  We
have a Tissue Tek VIP closed processor.  Has anyone ever experienced any
problems like this?  We had a similar issue a few weeks ago.  I thought
the contaminant cells may be from a bladder tumor, which had multiple
sections submitted.  In this instance the cells showed up days work of
the bladder tumor, and in the following days work also (though the
pathologists could not say for sure the cells were from the bladder
case).  We changed our formalin solutions in the processor and the
problem did not present the next day.  We also started putting all
bladder tumor specimens in the microcassettes, to prevent tissue from
escaping.  Has anyone had any problem like this, or does anyone have any
ideas on how to prevent this in the future?  We had not seen this
problem until these past two incidences, and this tonsil problem is
particularly strange to me because we process tonsils and GI specimens
in the same workload on a regular basis and have never had this issue
before.  Any help is appreciated!  

Thanks!
Brandi


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FW: [Histonet] GI, Uro, or Derm Path Lab Set Up

2010-02-05 Thread Thomas Jasper
For all.
tj 


-Original Message-
From: Thomas Jasper 
Sent: Friday, February 05, 2010 1:34 PM
To: 'Blazek, Linda'
Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up

Dear Linda,

I have received private responses similar to yours.  Seeing that you've
posted for everyone I am compelled to respond.  Let me start out by
saying my issue, complaint, concern - whatever you want to call it
obviously is not aimed at an operation such as yours.  As a matter of
fact my service is a stand-alone Histo/Cyto practice.  We are full
service, accredited and located 2 blocks from our main med center.  Our
situation is like a path department in a hospital, minus the politics,
etc.  I'm saying this because I am not against, private, above board,
accredited operations, which are run ethically and free of ulterior
motives.  I apologize if anyone took offense, but I think it should be
clear if your not what I've described, then your not it.

Secondly, it was not my intention to vent in an uneducated, juvenile and
unprofessional manner.  Quite the opposite as a matter of fact.  Here's
what I know about so called "pod" labs, which by the way does not lump
everyone into one group.
I interviewed a candidate 2 years ago that came from a "pod" lab.  I was
appalled as she described the working conditions, facilities, lack of
equipment and support she received with this service.  Improper
ventilation and plumbing, inadequate space, under-qualified assistance,
unreal expectations from those in charge.  This was an unethical
practice at best and I'm at a loss to understand how it was allowed to
legally operate.

Through professional contacts I've been made aware of more and more
"fly-by-night" ventures which are based on a lucrative financial reward
for a few at the top. This is at the expense of facility and technical
support.  Along with this comes patient care risk.  Nine months ago I
lost an excellent tech to a "pod" lab.  Within 2 weeks he was calling
and e-mailing back, regretful of ever getting involved with this
operation.  He was lied to, overworked, underpaid and totally mis-lead
by a poorly conceived and financially unbalanced venture.  Fortunately,
he will be rejoining us next week and he basically cannot get away from
these people quickly enough.

Now in our area plans for a "pod" lab are in the works.  This facility
does not have the space, ventilation, plumbing or staff.  The group
behind this venture has a history of upsetting behavior in the medical
community.  Let's just say they're controversial at times.  As I
mentioned previously they've advertised for a tech but I have no idea
how or what they think they're going to pull off.  I'm sorry but there
are a ton of red flags here.  The only reason I can think of that these
operations are allowed to exist is that they skirt regulations somehow.
And this has been alluded to by others posting on this list.  My point
is that I personally cannot abide subpar operations, that exist only to
line the pockets of unscrupulous parties.  Which in turn potentially
leaves patients and well meaning staff in it's wake.  

If this is not you, great, more power to you and carry on.  If this is
what you're involved in consider yourself put on notice and remember
karma can be a real bitch.

Hopefully clear and not misunderstood,
Thomas Jasper

-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com]
Sent: Friday, February 05, 2010 12:33 PM
To: Thomas Jasper; Nails, Felton
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up

Dear all that are blatantly lumping all private or office specific GI,
GU or Derm labs into one lump.  It is very uneducated and juvenile to
vent in such an unprofessional manner.
You are not very well informed and/or ticked off at losing revenue from
your hospital facility.
Our facility complies with and exceeds all standards set by CAP, CLIA,
OSHA and COLA.  
We are inspected at the required intervals by both COLA and CLIA.
We do the required air quality validations. 
All of the staff is certified and participates in annual continuing
education.  
Our main goal is patient care.  Our pathologist interact with our
clinicians on a regular basis.
Our Quality Improvement, Quality Assessment and Quality Management is
excellent.  
We have received the Laboratory Excellence Award from COLA and high
praise by CLIA.  
All of our equipment is state of the art and regular maintenance is
performed.
There isn't a member of our team that would not either themselves or
refer a member of their family to our facility.  
I don't think there is an employee here that wants to leave here and go
back to the hospital facility and have to deal with the ever increasing
drive to put out more and more work in less and less time.  Our goal
here is quality not quantity.
Th

RE: [Histonet] GI, Uro, or Derm Path Lab Set Up

2010-02-04 Thread Thomas Jasper
I am in total agreement with both Richard and Felton here.  Sorry
Timothy if you are reading this but to me the bottom line is patient
care.  And in the end set-ups like this hurt patients.  These "labs" and
I'm using the term loosely are put in place to line the pockets of a
small number of people at the top of a pyramid.

I guess someone will always work to put things like this together if
there is a demand.  But please realize, if this is what you're involved
in, the reality is...

~ Questionable facilities - Cramming professional, OSHA legal and
ergonomically correct lab operations into small office spaces is a poor
idea at best.  Proper plumbing, ventilation and lab space are serious
considerations and fall by the wayside when these operations are put
together.

~ Qualified personnel - All of us posting on this list understand the
fact that qualified, competent Histology Lab personnel are in demand and
difficult to find.  The wage for qualified, competent technical
expertise does not fit into the plans of the originators of these
operations.

~ Proper equipment and instruments - While the aim of these operations
is limited in scope, basic functional equipment and instruments are
required.  In the overall scheme of things, pathology is still a
bargain.  However, money must be spent to properly equip a competent
working lab with modern instruments.

I could go on but that makes the basic point.  And Timothy, lest you
think that I'm shooting from the hip I assure I am not.  Like most
people on this list, I am a serious, diligent professional.  I have
interviewed people from "pod" labs that have worked under horrible
conditions.  I am also aware of a "pod" lab trying to get off the ground
that shouldn't have an ice cube's chance in hell.  The plans for this
lab do not have proper plumbing, ventilation or space and there is no
equipment.  While a position has been advertised I cannot think of a
single tech, worth his or her salt, that would consider associating
themselves with such an ill-conceived venture.

One last point - don't forget Timothy, that one day you may be that
patient.  Or it may be your mother, sister, daughter, wife, son,
friend...I'll stop there.  Every block, every slide, every piece of
tissue that I or any of my staff encounter is a precious patient...a
human life.  So trying to dumb everything down, cut corners and make a
small number of people wealthy in the process is an irresponsible and
utterly ridiculous risk.  The professionals in our field have worked
hard for a good number of years to come out of the basement spaces and
after-thought little nooks and crannies and other poor facilities to do
the highly skilled work this profession demands.  I for one will never
abide going backwards and I don't believe people of good conscience and
humanity will either.

Sincerely yours,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails,
Felton
Sent: Thursday, February 04, 2010 12:57 PM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay
Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up

Especially when these physician hire unqualified people to run these
labs and flood the histonet with their uneducated questions.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Thursday, February 04, 2010 2:29 PM
To: histonet@lists.utsouthwestern.edu; Timothy Jay
Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up

We don't need anymore pathology laboratories.  What we need is support
of existing laboratories, especially hospital-based labs.  GI and GU
physicians are "Cherry-picking" the technical revenue that should be
going to hospital labs.  Let's reform health care; make it more
efficient and less expensive.  We don't need to be putting more money in
clinicians' pockets.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> Timothy Jay  2/4/2010 1:28 PM >>>
For those needing help putting an in-office path lab together whether
you are GI, Urology, or Derm please send me an email at tja...@yahoo.com
or call me at 775-830-1591. I have a consulting business that
specializes in putting these labs together. References provided upon
request. 
 
Timothy Garcia-Jay, MHA



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RE: [Histonet] Staffing for IHC

2009-12-17 Thread Thomas Jasper
I would totally agree Angela.  Without knowing all the details, I'd bet
these people are plenty busy all day everyday.  I'd worry about burning
them out.  And what happens when one or the other is sick or needs a
vacation?

Tom J

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, December 17, 2009 9:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staffing for IHC

I'm hoping some of the larger hospitals out there will share their data
with me.
I'm curious as to how labs are staffed for doing IF/IHC/CISH. 
A small portion of our staining protocols still require pretreatment by
hand, but we run instruments that do the pretreatments on board for the
majority of our stains.
We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are
cutting and staining between 200-350 slides per day. I think this is a
high volume for only two people. What do you all think?



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FW: [Histonet] VENTANA AUTOSTAINERS PLS

2009-12-11 Thread Thomas Jasper
One more time! 

-Original Message-
From: Thomas Jasper 
Sent: Friday, December 11, 2009 3:24 PM
To: 'Jimmy A'
Subject: RE: [Histonet] VENTANA AUTOSTAINERS PLS

Jimmy,

What the...!!?  I don't think anyone is going to be able to help you.  This is 
a vague request and I'm beginning to wonder about the sincerity and credibility 
of it.  

My suggestion - Either get real or please get off this list and leave folks be.

Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services Bend, Oregon 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jimmy A
Sent: Friday, December 11, 2009 3:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] VENTANA AUTOSTAINERS PLS











 
  Hi,
    I am looking for a lab that can allow me to spend one or two days to 
properly see how the ventana autostainer works.
 I am an histotech working here in the US. I am interested in learning  the 
operation of the ventana immunostainers. I will appreciate it, if one of 
the histology/ihc labs could grant me this great favour.
  Hoping to hear from you guys asap.
 
 Jimmy.




  
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RE: [Histonet] Bachelor's Degrees

2009-12-05 Thread Thomas Jasper
One more reason to consider "carefully" before throwing support to state
licensure where it does not exist.  I feel sorry for you Nathan and I'd
like to have someone explain the upside of licensing to you.  It seems
it's not about having a license (like a driver's license) to practice
histology.  I fear it's just more about fattening state coffers and
adding another level of bureauracracy to things.

If you are educated (as you are Nate) and if you are academically
eligible to sit for the registry exam.  And if you can satisfactorily
pass the exam, what has state licensing got to do with it?  Are you a
better histologist in New York because you're "licensed" as opposed to
your neighbors in PA, for example who aren't?  I think not.

Does licensing prove something that science degrees and registry
certifications do not?  Maybe I just don't get it.  And I'm not trying
to pick a fight here with the supporters of licensing.  I just haven't
heard a good convincing argument for it yet.  I'm also quite certain
that even though monetary compensation has improved somewhat, the last
thing most Histologists need is another payment.  The privilege to work
in a certain state, which is paid for (by you) nothing more?!  

I suppose some kindly employers out there somewhere could pay for
it...good luck with that.  Here's an idea, let's say you're degreed and
registry eligible and/or have passed your board exam(s) and are
certified.  How 'bout the state says you've met the qualification for
licensing, here you go!  Nate you are degreed and certified and in my
book and in the book of the current state I live in - Oregon - and the
states I've worked in - Wisconsin, Michigan and Minnesota - you are more
than qualified to work.  I for one would not hesitate in the least to
consider a person such as yourself for employment.  Again you are more
than qualified, even though you are "unlicensed". 

I guess I just don't understand how credentialing - degrees and
certifications - aren't enough, but licensing is the magic ticket to
better science/medicine/patient care/whatever.  I'm sure some folks out
there will bring on the firestorm, but again Nathan I feel sorry for you
and I don't see the reasoning behind this.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan
Jentsch
Sent: Saturday, December 05, 2009 11:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bachelor's Degrees

Paula,
Let me tell you that this is an extremely frustrating point for me not
for getting a job but for getting a license in New York State (which is
related because I'm technically supposed to have a license to work).
Despite the fact that I have a B.S. in a science field and have been
working competently at my job for almost two years now, the state wants
me to have an A.S. in histotechnology to get my license.  They won't
even consider HT certification as sufficient.  If a collective group of
experts in the fields of laboratory science and pathology say I'm
qualified, why isn't that good enough for a bunch of beurocrats who
can't even manage the pocket book of our state.

Nate
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RE: [Histonet] (no subject)

2009-12-03 Thread Thomas Jasper
Hi Jeri,

He should be fine.  Passing the HTL demonstrates considerable knowledge
which is applicable to the clinical (human) lab world.  Also, I'm of the
opinion that animal tissue - in general - is more difficult to section
than human tissue. 

Not actually knowing the person - which makes a difference - and with
the information you've provided, again, he should be fine.

Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
j...@opssearchgroup.com
Sent: Thursday, December 03, 2009 11:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Histonetters,

I am hoping you folks can provide an opinion.  I am assisting an
individual who has a Biology degree, HTL certification and for the past
3 years worked in the histology section of a private lab where they cut,
embedded and stained animal tissues. He would like to transition to a
hospital lab.  Is there any reason his skill set and knowledge would be
incompatible with his desired new ambition?
 
Jeri Vitello
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RE: [Histonet] a basic question about immunohistochemistry

2009-11-28 Thread Thomas Jasper
Dear Salim,

As you have been informed, doing immunohistochemistry is possible on
this tissue.  After all it's possible to do IHC on any tissue whether
the conditions you want to test under are ideal or not.  

Being chastised on this list and calling your work "bad science" is
totally out of line and certainly does not help you out.  I think some
people would do well to reserve judgment, particularly when there's no
way they can fully understand what's going on with your project.  Having
worked in research myself, I completely understand that animals will
die, at the most inconvenient times, during a study.

First of all you should incorporate the data about the animal dying into
your study notes.  Secondly, there's no harm in running the IHC on this
animal's tissue.  You can use the results comparatively with results
from some perfused tissue later on.

I don't know Salim, some people might call it damage control, or making
the best of a less than ideal situation.  Again, I don't know exactly
what you're working on but it seems there's information worth gathering
despite the circumstances.  I also understand that it's probably next to
impossible to carry out experiments and research alone.  Having reliable
staff assist you is not unusual either.  

Good luck to you,

Tom Jasper


Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Salim
Yalcin Inan
Sent: Friday, November 27, 2009 2:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] a basic question about immunohistochemistry

Dear All,

Because I am new in immunohistochemistry, I have a basic question about
it.
What if your rat dies in the evening or in the weekend, which you are
doing a chronic experiment and need to collect brain tissue for
immunohistochemistry? And let's say, the staff did not noticed it to
inform you on time. Several hours passed since your rat died. There is
no way to do perfusion. Is it still possible to do immunohistochemistry?
Thank you very much in advance.

Best regards,

Salim Yalcin Inan, Ph.D.
(post-doctoral fellow)
Department of Clinical Neurosciences
University of Calgary
syi...@ucalgary.ca


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[Histonet] Floors

2009-10-24 Thread Thomas Jasper
My thanks to everyone for their input about cleaning lab floors.  Much
appreciated.
 
Tom J.
 
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
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FW: [Histonet] Floaters in Waterbath

2009-10-23 Thread Thomas Jasper
One more time.
tj 

-Original Message-
From: Thomas Jasper 
Sent: Friday, October 23, 2009 10:41 AM
To: 'Stella Mireles'
Subject: RE: [Histonet] Floaters in Waterbath

Others may mention this to you...and it can get a little political.  Do
not discount the grossing bench.  Whether it's the work of a PA,
Pathologist, or other qualified lab staff, the grossing bench should be
kept as clean as possible between cases/specimens.  I mention the
political side because sometimes it gets a bit touchy...histologists may
not be in the best position to broach the subject with certain higher
level personnel.  Especially when the grossing may very well be done
under the supervision of said higher level party.

>From a patient care standpoint, etc., this definitely should not be an
ego-bruiser, as we are all human and make mistakes.  But I'm sure most
of you know what I'm talking about and probably have experienced
something similar at sometime in your careers.

One tip I learned from a pathologist, was to keep a clean sponge handy
while grossing.  This helped a lot, especially with keeping forceps
etc., clean in between cases/specimens.  Lastly, there are pathologists
and PAs out there that keep their egos in check and we are thankful to
them.

Tom J.

Thomas Jasper HT (ASCP) BAS
Central Oregon Regional Pathology Services Bend, Oregon 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella
Mireles
Sent: Friday, October 23, 2009 7:11 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floaters in Waterbath

I know we have all had some problems with floaters in our waterbath at
some point in our microtomy career.
Our doctors are very picky and I need some tips on keeping an immaculate
clean waterbath, but not sacrificing the speed in a regular routine lab.
We use the pyrex waterbath and paper towels for wiping our area.

Thanks
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RE: [Histonet] Inspection question

2009-10-22 Thread Thomas Jasper
The reason for that being? Pray tell...

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, October 22, 2009 9:08 AM
To: histonet; Patti Loykasek
Subject: Re: [Histonet] Inspection question

Yes!
René J.

--- On Wed, 10/21/09, Patti Loykasek  wrote:


From: Patti Loykasek 
Subject: [Histonet] Inspection question
To: "histonet" 
Date: Wednesday, October 21, 2009, 6:15 PM


Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that 
plants in the histology laboratory are a possible contamination hazard & must 
be removed? Just wondering.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the 
intended recipient, please contact the sender by e-mail and destroy all copies 
of the original message, or you may call PhenoPath Laboratories, Seattle, WA 
U.S.A. 
at (206) 374-9000.


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RE: [Histonet] Inspection question

2009-10-21 Thread Thomas Jasper
Furthermore, most any plant (unless I'm missing something here) is
beneficial for air quality...plants want CO2 and we appreciate their
oxygen.  I sure hope some inspector somewhere hasn't taken issue with
plants your in the lab.

Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor 
Central Oregon Regional Pathology Services
Bend, Oregon 97701  
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, October 21, 2009 3:17 PM
To: Patti Loykasek; histonet
Subject: RE: [Histonet] Inspection question

No... 
And spider plants and some others help remove formalin fumes. That was
published somewhere. j 


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Wednesday, October 21, 2009 18:15
To: histonet
Subject: [Histonet] Inspection question

Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector
note that plants in the histology laboratory are a possible
contamination hazard & must be removed? Just wondering.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any
unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call
PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


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It may contain information that is privileged and confidential.  Any
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[Histonet] Floor Cleaning

2009-10-21 Thread Thomas Jasper
Hi Folks,
 
Got a floor cleaning question for you.  We are transitioning to a new
floor cleaning crew in our histo lab.  I've been asked by the building
manager to elicit some opinions about products to use and/or techniques
to best get paraffin up off of linoleum.  We were fine with our previous
cleaners, however, the thinking around here is maybe it could be done
better?  Is a machine required or not?  Could we do it at a lesser cost,
and in a more bio-friendly way?  Is there an easier and simpler method?
 
Anyway this certainly is not the hot issue of the day for Histo-net.
However if anyone would care to share anything on this it would be
appreciated.
 
Thanks,
Tom J.
 
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
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RE: [Histonet] MUM 1 antibody

2009-09-29 Thread Thomas Jasper
Hi Martha,

We run MUM 1. We get it from Cell Marque and run it on our Ventana
Benchmark XT.  We use a tonsil control and our hemepath likes it just
fine.  I realize you're running the Bond.  Don't know the particulars
about protocols for the Bond.  We incubate for 32 minutes at 37 degrees
(if that helps).  You could start there anyway. 
Good luck,
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Tuesday, September 29, 2009 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MUM 1 antibody

I have been asked by my hematopathologist to work up MUM 1  antibody.
Does anyone have any suggestions as far as vendor?  Any additional
advice for the Bond stainer would also be appreciated.
Thanks in advance for your help.
 
Martha Ward
Wake Forest University Baptist Medical Center
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RE: [Histonet] What percent of HTL's do not have a BS degree?

2009-07-14 Thread Thomas Jasper
Hi Steve,

I've got no statistics to offer you...just an observation.  I would say
that finding an HTL, without a Bachelor's degree is akin to the
proverbial needle in a haystack.  Anyone that obtained their HTL,
if/when they could be grandfathered in, is likely to be retired or close
to it.  First of all, most folks that went the OJT route for
certification were eligible to sit for the HT only (to my knowledge).
I've never met anyone with an HTL that did not have a Bachelor's as a
pre-requisite.  I've been doing histology for ~25 years.  I've met
people from all over the country and various parts of the world.  Truth
is there isn't an abundance of HTLs out there.  Unlike the Medical Lab
world, with the basic differences between MTs and MLTs, anatomic path
does not exactly mirror that with the HTL and HT.  It's true the MT and
HTL both require a Bachelor's, but responsibilities in most labs, etc.,
generally do not hinge on someone being an HT vs. an HTL. 

A person like myself is probably more common (Bachelor's and an HT).
Unless you know of someone in particular; that you want to hire, with an
HTL without a Bachelor's, I wouldn't waste time trying to justify it.  I
guess the bottom line is if you want an HTL, that person will almost
assuredly have a Bachelor's.  If you want to hire someone without a
Bachelor's that is certified (HT) you'll have better luck.  I think
having an HTL is a great thing.  I honestly have never pursued it
(though eligible) as the circumstances of my career would not have
rewarded me for doing so.  As a matter of fact some employers may look
at it as an over-qualification, or at least no justification for better
pay, perks or responsibility.  Again, no slam to HTLs just the way
things are, at least in my experience.

If you want to hire people without a Bachelor's I would definitely
pursue HTs.  HTs have been doing a great deal of very good work for
years in this field.  And it sounds like you're viewing the Bachelor's
thing as limiting factor more than the HTL itself.

Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Monday, July 13, 2009 9:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] What percent of HTL's do not have a BS degree?

I'm trying to find some solid statistics to justify being able to hire
HTL (ASCP) candidates who do not have a Bachelor's degree.  I am
contending that requiring the candidate to have a Bachelor's degree will
eliminate a substantial number of very qualified people.  Does anyone
have any solid references to support my position.
 
Thanks,
 
Steve
 

Stephen A. Feher, MS, SCT (ASCP)

Pathology Supervisor

Catholic Medical Center

100 McGregor Street

Manchester, NH 03102

603-663-6707

sfe...@cmc-nh.org <mailto:sfe...@cmc-nh.org> 

 
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RE: [Histonet] OSCAR Antibody

2009-07-08 Thread Thomas Jasper
Hi Maria,

We run OSCAR on Ventana Ultraview - CC1 Mild, 37 degrees C, 16 minute
incubation.  We get the antibody from Covance - product #SIG 3465-16.
This comes as a 6ml predilute.  Our docs like it quite a bit, and order
it on a regular basis.
Hope this helps.

Regards,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria
Katleba
Sent: Wednesday, July 08, 2009 1:58 PM
To: Histonet
Subject: [Histonet] OSCAR Antibody


Can anyone tell me where I can get a really good OSCAR antibody that
works with Ventana Ultraview detection kits?

I need a pre-dilute if possible.

Thanks,

Maria Katleba HT(ASCP) MS
Pathology Dept. Mgr
Queen of the Valley Medical Center
Napa CA 94558
707-257-4076

Notice from St.Joseph Health System:
Please note that the information contained in this message may be
privileged and confidential and protected from disclosure.


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[Histonet] Histologist Position in Bend, Oregon

2009-06-09 Thread Thomas Jasper
Fellow Histonetters,
 
A position has opened for a Histologist here in beautiful Bend,
Oregon...
 
Central Oregon Regional Pathology Services - Bend, Oregon seeks a
full-time Histologist, HT, HTL or registry eligible preferred.
 
Responsibilities include - Embedding, Microtomy, Routine Staining,
Automated Special Staining and Immunohistochemistry.  Excellent
benefits, including health insurance, retirement plan and competitive
salary.  Potential sign-on bonus as well.
 
Bend is located in central Oregon at the base of the Cascade Mountains.
World class outdoor activities abound, including biking, hiking, rock
climbing, fishing and camping.  Phenomenal alpine and nordic skiing
available October through May.  Bend is uniquely situated with the
Cascades to the west and the high desert to the east.  Although in the
Pacific Northwest, Bend enjoys warm, clear days and cool evenings most
of the year.  Winters are mild with a clean environment and accessible
wilderness.
 
In about 30 minutes you can reach the scenic beauty of canyons and rock
formations in the high desert.  Crater Lake national park is
approximately 100 miles south and the Pacific coast can be reached in
just over 3 hours.  The lush Willamette Valley lies between the Cascades
and the coast and is stunningly beautiful as well.
 
Many wonderful restaurants of varied cuisine are located all over town,
outstanding wine is produced locally and 4 microbreweries produce top
notch beer.  Bend has a community symphony orchestra, a diverse musical
scene and many art and cultural events.  The Univ. of Oregon and Oregon
State Univ. are in close proximity, and Portland is only a few hours
away.
 
We are a progressive, friendly lab and a great place to live.  Contact
us at -
 
Central Oregon Regional Pathology Services
1348 NE Cushing Dr.
Bend, OR 97701
Attn. Pam Sylvester or fax resume to (541)693-2648
 
or, you may contact me as well, Tom Jasper at (541)693-2677
 
Thank you,
 
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
tjas...@copc.net
541/693-2677
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RE: [Histonet] Disposal of Formaldehyde

2009-03-13 Thread Thomas Jasper
You are correct Nanette.  Waste water treatment is under the jurisdiction of 
local regulations from place to place.  What's allowed in one place may not be 
in another.  Seems it depends on what various waste treatment facilities have 
the ability to handle.  It also seems, historically what has happened to water 
in a certain regions and what folks will tolerate.  I'm not trying to excuse 
pollution, I'm just stating what I understand exists.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette
Sent: Friday, March 13, 2009 10:24 AM
To: 'rjbu...@yahoo.com'; histonet; Jessica Piche
Subject: RE: [Histonet] Disposal of Formaldehyde

I have to disagree.  Although it certainly is not environmentally friendly to 
dispose of formalin down the drain, it is not prohibited.  At the hospital 
where I worked, a city inspector came to the lab and and designated in writing 
how many gallons of formalin could be put in the sewer system per day and we 
had to keep a written log of how many gallons we could dispose of and could not 
go over our amount or we would be fined.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, March 13, 2009 11:27 AM
To: histonet; Jessica Piche
Subject: Re: [Histonet] Disposal of Formaldehyde


Jessica:
It is absolutely prohibited, "verboten" to dump formalin into the sewer system. 
Try to check out other recommendations by this "consultant" and take them with, 
at least, a grain of salt. That guy does not know what is talking about and 
could get your lab in serious trouble. René J.

--- On Fri, 3/13/09, Jessica Piche  wrote:

From: Jessica Piche 
Subject: [Histonet] Disposal of Formaldehyde
To: "histonet" 
Date: Friday, March 13, 2009, 10:11 AM


Hi All,
We have a question regarding the disposal of formaldehyde. We were told at our 
hospital that a consultant said it was okay to dump formaldehyde down the 
drain. I believe they said it was okay to dump 15 gallons or so a day! We are 
not to fond of this idea and would like to know what everyone else is doing. 
How is everyone disposing of their formaldehyde? We would be especially 
interested in what other hospitals in CT are doing.
Thanks,
Jessica Piche-Grocki, HT(ASCP) ___
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[Histonet] Dako

2009-03-12 Thread Thomas Jasper
OK,
 
There've been plenty of shots taken at Dako...and from what I can tell
rightly so.  I agree with the statements about the "good old days" and
all, and I would still like to believe Dako is a decent antibody
company.
 
So here's my question - How 'bout it Dako?  Are you going to take all of
this lying down?  Do you have any response(s) at all?  You (collectively
as a company) probably owe a lot of these folks some sort of
explanation.  I find it hard to believe that Dako has opted to adopt a
business model, which (from all appearances) has taken a path of
self-destruction.  And I don't care who the parent company is now
(Danaher?) these anecdotes exemplify irresponsible handling of business.
It certainly isn't necessary to have attended Harvard (insert your
favorite) business school to draw this conclusion.
 
I also believe that competition is good for the marketplace.  By having
Dako, a one-time leader in the field, basically "tank" isn't good for
anyone.  I'd like to see something salvaged here, but it's not up to me.
 
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology 
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
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RE: [Histonet] question of the day - embedding

2009-02-17 Thread Thomas Jasper
Tracy,

I used to work at the same (nameless) place with Mr. Saby and I concur.  In my 
opinion your colleagues are potentially causing harm to the tissue specimens 
(especially small, delicate ones) while you are not.  Hot paraffin, specimens 
of various tissue types (particularly animal) and the resultant heat transfer 
is less than optimal.  Consider...why is it so important to get your specimens 
off the processor(s) shortly after the run(s) have been completed?  
Answer...you don't want to "cook" them in liquid paraffin on the final station. 
 So why cook them in a holding tank full of liquid paraffin while you embed?  
Also, the tissues (ideally) have been properly fixed, processed and 
infiltrated.  No harm will befall them without liquid paraffin, until they are 
embedded.

Good luck,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Tuesday, February 17, 2009 3:04 PM
To: Tracy Bergeron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] question of the day - embedding

Tracy-

Where I used to work (at a place that shall remain nameless), we always kept 
our tissue being embedded in hot paraffin in the holding chamber.  Most of my 
work has been with animal tissues.

Where I work now, we don't.  And I do bellieve you are right.  If the tissues 
remain in hot paraffin, the heat transfer rate is very high, and the tissues 
continue to "cook" even when the chamber temperature has been reduced (as close 
as feasible) to the melting point of the paraffin.  I have seen little effect 
on the tissues of longer-than-I-would-like time in the holding chamber without 
paraffin.  Without the paraffin, the tissues do not get that direct heat from 
the melted paraffin and survive delay much better.

In short, I agree with you.  Not keeping the tissue in hot paraffin does not 
only not damage those tissues, it allows more flexiblity in your embedding 
times.

Joe Saby, BA HT





From: Tracy Bergeron 
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, February 17, 2009 4:14:46 PM
Subject: [Histonet] question of the day - embedding

Hi all question/dilemma of the day.

        I have been of the view that the longer tissue sat in melted paraffin 
the harder it got, especially animal tissue.  So with that said, for the past 
nearly 10 years I have not used melted paraffin in the holding chamber of the 
embedding center.  I just keep the chamber warm, and work that way.  Thus 
keeping the tissue from continuing to cook and harden in the wax.

        Everyone else I am currently working with has never seen the method I 
use, and firmly believe that this causes harm to the tissues if they are not in 
paraffin.

        Thoughts ideas etc.  I am dying to know if I am the only one that 
worries about length of time that animal tissue sits in paraffin.

Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct:  617-914-1115
Fax:  617-679-3208
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RE: [Histonet] Problem with Schiff's reagent

2009-02-14 Thread Thomas Jasper
Hi Paul,

Some people may think this is taking the "easy" way out, but...you might
consider purchasing some commercially prepared Schiff's.  It will
(should) be consistent product (consistently produced) and the
manufacturer has the responsibility of QC'ing the product before it is
shipped and sold.

There are any number of reliable companies handling Schiff's (I would
not favor one over another).  You could type "Schiff's" into your search
engine and I'm certain you'd be able to contact a supplier.  This may be
an answer for you.

Good Luck,

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor 
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
tjas...@copc.net 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pbru...@siue.edu
Sent: Saturday, February 14, 2009 9:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problem with Schiff's reagent


Good Morning!

I'm a newcomer to the list, and I'll start by pointing out that I'm not
a histologist by any means.  I am a freshwater ecologist, and we're
trying to study mucus secretion behavior in freshwater snails.

Last Spring and last summer, we developed a process whereby we could
visualize snail mucus trails on glass slides using a periodic
acid-Schiff's reagent staining technique.  But now, in following the
same protocol as used last year for making our own Schiff's reagent, I
cannot get the final solution to filter out clear.

Recipe I'm using:
900 ml boiling water
10 grams basic fuchsin
25 ml concentrated HCl acid (12 M)
40 grams sodium metabisulfite
(this is essentially Sigma Aldrich's ratios, I think)

Let this sit for 24 to 72 hours, take 100 ml aliquot, add 0.75 - 1.0
gram ground activated charcoal, stir for 10 minutes, filter through
filter paper then through GF/C glass fiber filter.  Last summer I got
nice, clear (slightly
yellow) and very active Schiff's reagent.

But now I cannot seem to get the filtrate to be clear.  Even after 10
minutes exposure to ground activated charcoal and filtering, the
filtrate remains bright orange to dark red and it does not seem to stain
mucus trails very well.

All the reagents are the same as those used last summer (i.e., less than
7 months old; although the HCl is a bottle several years old from a
different lab).

Anyone have any troubleshooting suggestions?  I don't know the chemistry
very well, but the sodium metabisulfite is used for "decoloring" the
initial solution, right?  So is the metabisulfite not working for some
reason now??

Any help/suggestions would be greatly appreciated.

Cheers -

Paul

Paul E. Brunkow, PhD
Department of Biological Sciences
Southern Illinois University Edwardsville
Edwardsville, ILUSA
-
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RE: [Histonet] re: Certified Histotechs

2009-02-12 Thread Thomas Jasper
Thank you Jennifer, well stated.
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
tjas...@copc.net
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, February 12, 2009 9:16 AM
To: christopher.conl...@kp.org
Cc: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] re: Certified Histotechs

I would like too clarify the role of NAACLS in training histotechs for
certification. There are a number of different NAACLS accredited
programs in the US.  All of these programs meet the standards set by
NAACLS or they do not remain accredited.  Providing on the job training
(OJT) is NOT one of the requirements.  All of the programs have a
practical component to them.  The hospital-based programs will provide
the practical instruction as well as the didactic portion.  College
based programs will provide the didactic portion and some also provide
some practical portions and the student will be placed in a clinical
affiliate work site for further practical training.  Other college based
programs will place the students into internships for the practical
training after they complete the didactic portion, or a variation of
this.

One of the challenges that NAACLS accredited programs have is to get
clinical affiliate sites to place their students.  There are affiliation
agreements, forms, and certain standards that these sites must meet. 
Another challenge is to get comparable training between sites.  Not
everyone out there is willing to be a part of the training of these
students.  There are some employees that refuse to help train the
students or even let them observe them working.

The ASCP does not "frown" upon OJT people.  Route 2 (AS or equivalent
{with sciences} plus 1 year of histology work experience ) allows for
the work experience component for certification eligibility.  The ASCP,
upon much research and feedback from the "professional world" has
established a minimum education requirement.,  Applicants that applied
for certification under the old route 3 (high school and 2 years of
histology work
experience) had an HTexam pass success rate around 30% on the computer
portion. Many of those applicants did pass the practical portion.  It
was determined that in many cases a high school education does not
provide the foundation for the theory portion of the exam. 

The quality of OJT techs is not being called into question.  The
histology community has for many years demanded respect and higher wages
to rival nursing and medical technologists.  We are the only clinical
profession that does not require some form of certification or license.
In order to command the respect that the profession deserves we have to
set standards. 
 Certification is a way to set the minimum standard.  Is it perfect, no.

There will always be people that can test well and perform badly, and
visa versa.  That folks is life.

For those that are working in the lab now and are not certified there
are a couple of NAACLS accredited on-line programs to qualify for route
1. You can take college credit courses to work toward your degree to
qualify for route 2.

Encouraging high school students into our profession is great, but
encouraging them to forgo college to do it is a disservice.  The world
of histology has changed a great deal over the years.  There is more of
a demand for higher complexity testing that did not exist when I first
trained.  Someone mentioned that automation is taking over.  You still
need people to trouble shoot and QC.  What happens when there are
problems? 

Jennifer MacDonald
Director, Histotechnician Training Program Mt. San Antonio College 1100
N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdon...@mtsac.edu





christopher.conl...@kp.org
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/12/2009 06:44 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] re: Certified Histotechs







I find it smartly ironic that all these "Lab Managers", MBA's," Med
Techs", are so offended and defensive in regards to BOR(ASCP), for
histology? Why?
There are journeymen electricians. Many technical fields have
registration/certification. I worked for 10 years as a Phlebotomist, a
Deiner, a lab assistant, and I didn't get paid squat until I went to
I.U.P.U.I., graduated on the Deans list and then sat for the exam
(clinical and practical), passed the exam etc. Part of the reason
pathologists and clinical lab scientists and MBA managers are so
condescending about histology, is because, they want it to always be an
easy access career, and the medical field is like the animal kingdom or
a caste system, it has its own little system of who is who and how dare
you ask me that I am a (you fill in the blank). Also, they ca

RE: [Histonet] uncertified techs

2009-02-11 Thread Thomas Jasper
Steve,

You never needed to pay the $45 a year to the ASCP.  Once you were certified, 
you were certified.  No one can take that away from you. And $45 for a sticker 
wasn't insurance of anything.  That money went to supporting the ASCP and 
getting you a copy of their magazine.  I realize it's different today for new 
certificants with the CE requirement, which is actually not a bad idea.

To everyone else interested in this discussion,

I fall on the side of ASCP certification being the unifying factor.  And I've 
still not been convinced that state licensure (or whatever kind of licensure) 
is necessary.  We have a national standard with the ASCP-BOR, I see no need to 
re-invent the wheel here.  And I realize I'm probably treading ground somewhere 
here between the advocates for licensure and those who feel uncertified techs 
are good enough and if you have more training, education, etc., that's nice but 
not required.

Seems to me that whatever the field of endeavor, you will always find people 
doing exceptional work and people without a clue.  To paraphrase George Carlin, 
"Somewhere out there is the world's worst doctor, that's bad enough, but what's 
worse is that someone probably has an appointment to see him today!"  So, this 
may or may not have anything to do with degrees, certifications, licenses or 
special training.  I think the general public would like to believe it does, or 
why would people bother to frame all their diplomas, certificates etc., and put 
them on the walls of their offices?  It does seem logical that someone with 
more training, education and all would be a better tech.  The reality is 
sometimes that's true and sometimes it's not.  I like the idea of a well 
educated and technically skill person working in my lab, but everyone brings 
something different to the table.  Also, from what's been posted already it's 
obvious to me that life circumstances have dictated how things shook out for 
most people.  Everyone wants respect no matter which path brought them to the 
lab.  I think we can agree on 2 things here which are both less than optimal - 
1)Having a technically skilled person that's good at embedding, cutting and 
staining, but doesn't have a clue about chemistry, biology or much in the way 
of science -that's a problem! 2)Having a certified, bona fide, glorified (and 
possibly funk-defied)degree holding, so called, well educated person that can't 
walk and chew gum, let alone get any lab work done -that's a problem!  I don't 
have the answer, but I do know that having the state hit you up every year for 
$$??, just to keep a license seems wrong!

In the last year, I've hired 3 well educated techs, that will eventually take 
their registry exam.  I appreciate the education they've got.  The level of 
technical expertise I get from them is good to very good as well.  So, I'm 
lucky in that regard.  I do believe it was a mistake to eliminate the practical 
portion of the exam.  I can elaborate on that with anyone who disagrees if they 
wish, but this post is probably long enough for now.  Also, I'm sure this 
discussion can seem confusing and odd to our UK, etc., colleagues, but their 
social medicine makes for a completely different beast.

Thanks for allowing me to ramble.

Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/617-2831
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Coakley
Sent: Wednesday, February 11, 2009 9:47 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] uncertified techs

Thanks for all the input.  So why am I wasting my cash paying the $45 for an 
ascp sticker?
15 years ago I suppose I should have taken the extra time to become at least an 
MLT.
Oh well.
 
Thanks again ya all.  :)
 
Steve


  
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RE: [Histonet] tissue swelling out of block

2008-10-01 Thread Thomas Jasper
Hi Gudrun,

Is your friend soaking blocks in ice water before cutting?  This is
common in animal histology and over soaking before  cutting will cause
swelling.  Different tissue types will swell more quickly than others,
but over soaking can eventually affect all tissue.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
[EMAIL PROTECTED]

 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Gudrun
Lang
Sent: Wednesday, October 01, 2008 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue swelling out of block

Hi all,

a friend recently got a job in a veterinary histo and has some troubles
in cutting. He tells about paraffinblocks, where the tissue is swelling
out while warming like a small hill.  I referred to the possibilty of
taking up humidity that causes swelling. 

Do you know other causes for this problem?

 

Thanks in advance

Gudrun Lang




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RE: [Histonet] Humidity levels in the lab

2008-09-19 Thread Thomas Jasper
Julie,

I can't offer you any references, but here's something to consider.  A
cool mist humidifier in your lab.  We are in Bend, OR., on the dry side
of the Cascades.  Running the humidifier reduces static and makes
sectioning a bit easier.  No hard science with this, just a little more
atmospheric moisture. 
By the way, the humidifier I bought is a big blue and white penguin.
The mist streams out the beak.  Just looking at it makes you smile.  I
bought it at Target for ~ $40.00 - it works for us.
Have a good weekend.
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
[EMAIL PROTECTED]




-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
Randolph-Habecker, Julie
Sent: Thursday, September 18, 2008 3:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Humidity levels in the lab

Folks,

We just moved to a new lab space in a new building. We are currently
running at 45% or lower humidity. I am working with our facilities on
raising the level but I am looking for some references for what level we
should aim for in regard to paraffin cutting. Does anyone have some
ideas or places to look for information?

Thanks!!

Julie

Julie Randolph-Habecker, Ph.D.
Staff Scientist - Director
Experimental Histopathology Shared Resource Fred Hutchinson Cancer
Research Center 1100 Fairview Ave, N. DE-360 (Please note new location)
Seattle WA 98109-1024
206-667-6119
[EMAIL PROTECTED]

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