[Histonet] Looking for suggestions, ideas
Hi Folks, Here is our situation. In the not too distant future we will be receiving additional specimens from 3 dermatologists. What they need is to have these specimens processed, embedded, cut and H/E stained. Once we've got the slides coverslipped they are to be sent back the dermatologists. We are not doing the interpretation. What I'm looking for is an idea about getting these into our LIS (PowerPath) and designating them for return to this group. We should be able to capture the TC and need to account for the workload. I'm thinking we could prefix them differently at the time of accessioning and then create something in the LIS attached to that designation which only generates a TC. I'm wondering if anyone else does anything like this? I'm also interested in having this differing designation for efficient workflow. I don't want any of these making their way to our pathologists. I've thought of identification by color of block and a new prefix. Just not sure if/what others are doing, how difficult to implement for an LIS standpoint (separation of TC and PC) and any other considerations. Thanks, I know I've got all the superior minds out there. Your thoughts are greatly appreciated. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital 600 Mary Street Evansville, IN 47747 thomas.jas...@deaconess.com 812-450-2485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blade Rationing Follow-up
You could start with cost comparison of blades (since this whole thing started as a blade issue). Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. You can do the same for paraffin and slides as well as reagent alcohols and xylene. Again, these are things the manager should be getting after. Does your lab adhere to Lean principles? There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. It's a bit difficult to say as much depends on the nature and scope of your service. All situations are unique - do you send a lot of work out? Could you take it on in-house? Or are you trying to do things in-house that could be sent out and have a cost positive effect? Lots of questions for the manager... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Blade Rationing
-Original Message- From: Thomas Jasper Sent: Tuesday, June 18, 2013 8:52 AM To: 'Teresa Moore' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Teresa, I concur with the all the responses. It seems your lab manager is not grasping the technical reality you must work under. If you are being asked to save money on blades why not try some different brands or negotiate some better pricing? That is something the lab manager can work on. Also, I would think you are doing your best optimize the use of each blade. You should be able to get 3 good cutting areas per blade before they're spent. Another consideration is having some blades for facing in only. I'm guessing the manager is being pressured to cut cost. I would look in other areas and at other items. Blades are of too critical importance to mess around with much. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS AP Supervisor Deaconess Hospital Evansville, IN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blade Rationing
Teresa, I concur with the all the responses. It seems your lab manager is not grasping the technical reality you must work under. If you are being asked to save money on blades why not try some different brands or negotiate some better pricing? That is something the lab manager can work on. Also, I would think you are doing your best optimize the use of each blade. You should be able to get 3 good cutting areas per blade before they're spent. Another consideration is having some blades for facing in only. I'm guessing the manager is being pressured to cut cost. I would look in other areas and at other items. Blades are of too critical importance to mess around with much. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS AP Supervisor Deaconess Hospital Evansville, IN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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[Histonet] FW: Automated Microtome question
See below. Thanks, tj From: Thomas Jasper Sent: Tuesday, February 12, 2013 8:56 AM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: Automated Microtome question Hi Everyone, Question - Does anyone know if there is a regulation in place (OSHA, i.e.) about having automated microtome(s) available to histologists if requested? In other words; I was under the impression that after 2005 (I think...) if someone wanted an automated microtome the institution they work at is mandated to provide it to them. This is not to say that folks must use an automated microtome; just that it's there or would be obtained for them. I thought I'd heard something about this a few years ago. With the repetitive motion issues today I was thinking this was a regulation of some sort. Thanks in advance for the input. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital Evansville, IN This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] certification of histotechnologists
Janet and Richard, To answer your question...to my knowledge this has not been given consideration in the U.S.(I could be wrong). Perhaps you could explain this concept a little bit. I personally have a hard time understanding how an AP discipline (histology) is taught, mastered well enough and certified alongside clinical lab disciplines (microbiology, hematology, blood banking and general lab). To me this seems a daunting task. Am I to understand that in Canada and the UK someone certified in your medical laboratory programs is able to bounce around and work in the clinical labs and then be expected to show up in histology and work at a competent level there as well? I worked in a lab were an MLT came and helped us out now and then because she knew a little about histology. She did not cut sections too well and one day severely filleted her finger. Also, in a previous supervisory position I was asked by the general lab for help in flow cytometry from my tech specialist. He spent time down there helping out and worked in my lab as well (IHC specialist). His mind was about fried after 6 months or so and I felt it was an unfair and unrealistic expectation for him to perform at a high level in both areas. Maybe I'm not understanding this correctly. I believe the standard view in the US is that Histology and Cytology are close relatives in the world of anatomic path. The other medical lab disciplines, microbiology, hematology, BB, etc., have people floating around that understand the instrumentation and objectives of those areas. Seems it's fairly common for histologists here to assist and do testing for cytology (a bit of heme as well). Again maybe I just don't get the concept. Especially as our discipline develops and we find ourselves moving into the world of molecular pathology; not to mention the other areas of histology outside of human clinical applications. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, May 25, 2012 5:36 AM To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification of histotechnologists Same in UK, I have never understood why the separation in the Land of the Free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unregistered techs
Holy buckets, that's a shot below the belt! Must say, I'm quite surprised to see a comment like that from someone with 19 years experience. By the by, I understand the registered HT thing, what is a "Florida licensed HTL"? Is that something new? But I digress. I can see your point about the scam/bleed money thing, but that's another discussion. You think monkeys can be trained to do histology...well, you're entitled to your opinion. However, the validity of an opinion depends on its basis. In my opinion monkeys cannot be trained to do our job. I'm quite certain that everything I did in the lab today (before returning to my office, reading this post and writing my response) would be challenging for a lot of folks...pathologists included...let alone a smart monkey. I'm a bit confused seeing the name Scott Lyons in the post below, so I don't want to direct my response to the wrong person. If this is indeed you, Jay, I've read many of your posts in the past. In consideration of that, I'm thinking maybe you're exaggerating to make a point, maybe having/had a bad day or both. I agree there is no substitute for experience. And I agree that many people with advanced degrees can be all thumbs in a lab, or maybe have a hard time transitioning book learning into hands on action. Come to think of it monkeys are pretty dexterous...so maybe we're taking this all wrong. I'm not responding to "light someone up" or get into a war or words with. I'll just say that I hold those of us doing this work in high regard, monkey or not. And that includes you too Jay...I've not met you personally, but honestly you're no monkey. Regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Thursday, May 24, 2012 2:02 PM To: Kim Tournear Cc: histonet Subject: Re: [Histonet] Unregistered techs Scott Lyons sln...@yahoo.com Give me a break, HTs and HTLs do not make diagnoses or treat patients. I am a registered HT and a Florida licensed HTL with 19 years experience, I've done it all in the lab. I believe the certification and licensure of techs is a scam to bleed more money from people. Honestly, you can train a monkey to do our job. And I don't want to hear from everyone saying it's an art form, we are just as much needed as pathologists, blah, blah, blah... I work where they are hiring people from a masters degree program for histology with certification, THEY KNOW NOTHING. Experience it where it's at, whether certified or not, get off your high horse. > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pro-Par
Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested
Ray, Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. Kind regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: Saturday, January 28, 2012 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is l
RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin
Matthew, I am in agreement with you. I appreciate Peggy's comments and understand the wherefore and the why regarding this. Unfortunately, something has been lost. We also serve as a clinical internship site for students coming out of Clover Park in the Seattle area. This is a 9 week stint and a challenge considering all we'd like to expose the student to in such a short time. As you mention in your post: speed, accuracy and quality are paramount and we do our best to prepare folks for the real world. I too, am glad to have taken the practical. Again, it's unfortunate that the practical isn't practical anymore. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8b7976b131854abc8db236fab5026...@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sox 10
General question - Is anyone out there running Sox 10 on the Ventana Benchmark XT? Any dilutions or protocols would be appreciated. Thanks, Tom Jasper Central Oregon Regional Path ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
Hi Stella, I would be more than happy let you take this response to your lab management. First of all, just a bit of background - I have been a histologist for over 26 years. I have an Associate Degree in Histotechnology and a Bachelor's Degree in Applied Science. I am a registered HT (1986) ASCP certified #12664. I have worked in; university and VA clinical settings, pharmaceutical research, major medical and now independent clinical service. I have been in supervision for over 10 years and function as a working supervisor. I have been responsible for Cytology, Autopsy and Transcription as well as Histology. I served for a number of years as a safety officer. I have significant experience with immunohistochemistry from manual kits, using concentrated antibodies in multiple species application to running the latest in automated IHC. Having said that, the notion that a single histotech should be able to cut 100 blocks an hour is sheer lunacy! Expecting anyone to even attempt such an unrealistic goal is dangerous, irresponsible and ridiculous. I seriously question "their own research". I'm sure you couldn't sell it in a deli as it sounds like nothing more than bad baloney. According to my calculations, that would be 1 block every 36 seconds...let that sink in. You mention you are dealing with med techs. These med techs apparently have no concept about the realities of Histology. I am going to assume this is the case and you (Stella) obviously know better. I will lay out the basic problems and hope you are able to drive home the point. ~ Volume - 100 blocks per hour equals 1 block every 36 seconds...really? Can you make change for a dollar in 36 seconds, find your car keys and start your car? Now do this over and over and over again, hour after hour. Even 50 blocks an hour is insane. ~ Variety - Histologists cut blocks from every part of the human body (or animal or plant). The specimens can be big or small, thick or thin, hard or soft. They can be dry and brittle, full of sutures and staples, under-fixed and poorly processed. When sectioning you are subject to humidity, air currents, quality of the knife edge and specimen orientation (and you just gave me a whole 36 seconds). ~ Quality - This is the number one consideration in my lab and any lab worth its salt. Quality is not achieved in one block every 36 seconds. I just mentioned a list of variables and out of that a histologist has to produce a microscopic work of art, one slide at a time, every time. Any pathologist worth his or her salt will tell you that. If you aren't giving a good picture to that doctor, he or she is not going to be happy. You will want to figure in some additional time beyond 36 seconds for all the rework you're going to get. ~ Patient Care - Every histologist knows that a specimen/block/slide is a patient. That patient could be your mom, dad, sister, brother or some other loved one and must be treated as such (regardless of who it is). Trying to force histology work through at an impossible rate is practicing bad medicine. Is that how you would want your biopsy handled? If there is nothing more important than the patient, I think the patient is worth more than 36 seconds. ~ Safety - Safety is easy to practice and easy to ignore. What are we dealing with here...extremely sharp blades for one. The occasional histologist may be known to skirt a safety rule now and then. Don't get your fingers too close to the blade. With automated microtomes there are new and exciting technical features to consider from a safety perspective. Regardless of the situation, speed factors into safety. Existing stress factors combined with new ones for unrealistic speed is an accident waiting to happen. And there are other mental health considerations from undue stress. ~ Special Testing - Not only are quality sections required for standard hematoxylin and eosin staining. Quality sections are required for straight chemical, special staining, immunohistochemistry and other special procedure applications labs may run. For example, some pre-treatments or other protocol steps involved in IHC may be a bit harsh. To rush and produce less than desirable sections for any of these various procedures, due to unrealistic quotas is a bad idea. Once again all of the above apply to special testing. Well Stella, I don't want to write a book and I'm sure I've left out some valuable information. I used to have unionized techs working for me at my previous position. I don't know if that's the case for you. I can guarantee the union steward would've had a field day with this one. Also, this is the type of thing that OSHA loves to get wind of, along with any state agency that regulates labor. Please contact me if you'd like to speak about this further. Not to sound extreme, but there are other jobs and nice, reasonable people to work for. Kind regards,
RE: [Histonet] How many tissues an histo tech is suppose to cut per
Well Joanne, someone on the Histonet probably has a documented average. It is a difficult thing due to the amount of variables that exist and the differences from lab to lab. Again, in my humble opinion (and not knowing anything about your workplace) to me, working in a mid-sized clinical service and dealing with the variety of specimens common to a lab such as ours...if someone is cutting 25-30 blocks per hour, the sections are high quality and the errors are negligible (a subjective statement) I would consider that more than acceptable. Others may be of a different opinion, however I would be surprised if someone would think that one block per minute is reasonable and realistic (except for your employers). I'm sorry to hear that it took you 10 months to secure employment at your current "Roman galley" ship of a service. There are jobs available and not enough of us to go around. I'd seriously consider breaking the leg iron, abandoning ship and taking your chances in the wider world. There are a lot of nice, sane people out there and I'm sure you could find a place that would appreciate and treat you fairly. tj -Original Message- From: Joanne [mailto:joanne0...@comcast.net] Sent: Saturday, June 25, 2011 6:31 PM To: Thomas Jasper Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Tom, Thank you for your response. As it took me almost 10 months to secure a position, I'm not likely to be able to jump ship. Is there a documented average for a histotech's performance to be judged against? Again, thank you. Joanne ----- Original Message - From: "Thomas Jasper" To: "Joanne" Cc: Sent: Saturday, June 25, 2011 9:01 PM Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Joanne, In my humble opinion and without knowing anything about where you work, this expectation is ludicrous. I have a hard time believing that anyone, regardless of experience could attain the goal you've mentioned (including answering phones and running instruments). There are a multitude of reasons why this is absurd. Suffice it to say, I would be suspect of any work coming out of such an operation, not to mention those in charge and the poor souls trying to meet this goal. I seriously doubt you'll be able to have much say about things since you are so new. My advice...get out and find a job working for realistic people. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
Joanne, In my humble opinion and without knowing anything about where you work, this expectation is ludicrous. I have a hard time believing that anyone, regardless of experience could attain the goal you've mentioned (including answering phones and running instruments). There are a multitude of reasons why this is absurd. Suffice it to say, I would be suspect of any work coming out of such an operation, not to mention those in charge and the poor souls trying to meet this goal. I seriously doubt you'll be able to have much say about things since you are so new. My advice...get out and find a job working for realistic people. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC pos. & neg. control question
Pete, Can't argue with that. I think for the sake of expediency most clinical services run a "known" positive and a patient slide for negative. In the case of H. Pylori, for instance, we may cut a box of control slides and it's possible to go through the area where the organisms were. This also happens with controls that demonstrate positivity by other means epithelium, tumor, etc. We may have to re-run tests in these situations. I believe we are similar to many clinical labs in our reliance on known positives. tj -Original Message- From: pete.peder...@healthonecares.com [mailto:pete.peder...@healthonecares.com] Sent: Thursday, May 19, 2011 2:54 PM To: Thomas Jasper; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Thomas, Agreed, however, how can you say with certainty that the control is still good, or the antibody is still performing optimally? Hypothetically speaking, if you had a known positive control and ran it like a patient specimen (positive and negative) and had staining in the negatively stained control that you had been only running as a positively stained control prior, how would you proceed? What good is a positive control without if it is not treated identically as patient tissue. If you had none specific staining in a patient negative but is was also there in your known positive control which you stained negatively as well, then you could mark up to nonspecific staining to reagent or IHC user error. If the negatively stained positive control stains truly negative and the patient negative has nonspecific staining then you would know patient tissue is compromised or has been mistreated somewhere along the way because your positively stained and negatively stained positive controls demonstrate the staining was done correctly, correct? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -Original Message- From: Thomas Jasper [mailto:tjas...@copc.net] Sent: Thursday, May 19, 2011 1:39 PM To: Pedersen Pete; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pete.peder...@healthonecares.com Sent: Thursday, May 19, 2011 12:31 PM To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject
RE: [Histonet] IHC pos. & neg. control question
Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pete.peder...@healthonecares.com Sent: Thursday, May 19, 2011 12:31 PM To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Procedure for making Gram Control
Some sort of small, snack sausage of questionable quality. I'm not familiar with anything like that in Europe, but maybe you could determine that somehow. Good Luck! Tom Jasper Bend, Oregon -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Thursday, March 17, 2011 1:47 AM To: Walter Benton; Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Procedure for making Gram Control What would be the equivalent of a Slim Jim in Europe? The Netherlands to be more precise? Van: histonet-boun...@lists.utsouthwestern.edu namens Walter Benton Verzonden: wo 16-3-2011 15:07 Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] RE: Procedure for making Gram Control Making you own...not sure how to do that, but Slim Jim's work in a pinch. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [randi.ha...@horizonnb.ca] Sent: Wednesday, March 16, 2011 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedure for making Gram Control Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi --- Horizon Health Network Disclaimer ---This e-mail communication (including any or all attachments) is intendedonly for the use of the person or entity to which it is addressed and maycontain confidential and/or privileged material. If you are not the intendedrecipient of this e-mail, any use, review, retransmission, distribution,dissemination, copying, printing, or other use of, or taking of any action inreliance upon this e-mail, is strictly prohibited. If you have received thise-mail in error, please contact the sender and delete the original and anycopy of this e-mail and any printout thereof, immediately. Yourco-operation is appreciated.Le présent courriel (y compris toute pièce jointe) s'adresse uniquement àson destinataire, qu'il soit une personne ou un organisme, et pourraitcomporter des renseignements privilégiés ou confidentiels. Si vous n'êtespas le destinataire du courriel, il est interdit d'utiliser, de revoir, deretransmettre, de distribuer, de disséminer, de copier ou d'imprimer cecourriel, d'agir en vous y fiant ou de vous en servir de toute autre façon.Si vous avez reçu le présent courriel par erreur, prière de communiqueravec l'expéditeur et d'éliminer l'original du courriel, ainsi que toute copieélectronique ou imprimée de celui-ci, immédiatement. Nous sommesreconnaissants de votre collaboration. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] California Histology Society
Hello Histofolks, Am wondering if someone from the California Society would be kind enough to contact me regarding the state convention this year? I'd like to send one of my staff. I was under the impression that it commences sometime in May. I've checked the website and haven't found any information on this. My contact information is below. Thank you kindly, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High Complexity Testing
Completely agree with you Glen. However, do take exception to the initial comment on this thread about "a machine does all the work". The "machine does all the work" comment is very telling about what this pathologist (or group of pathologists) knows and does not know about running IHC. If the "machine does all the work" what do you need the IHC staff for? To me, that's like saying when a pathologist using image analysis, that the "machine does all the work". Granted you're going to have people with various skill sets and knowledge of histochemical processes, but comments (and attitudes) like that are degrading in nature. If the "machine does all the work" why doesn't this pathologist go to the nearest McDonald's and recruit from they're application files? Just trying to make a point here and tip my hat to everyone working diligently and intelligently even though you may have a "machine doing all the work". Tom Jasper -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 10:40 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA regulations > govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties when > necessary. > > It is "high complexity testing" because "testing personnel" in anatomic > pathology are pathologists (and the non-physician people performing gross > examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report results > of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If the > test is done properly [the responsibility of the technologist] then the > reading to the test is a visual determination that requires experience on > the part of the pathologist, but if the test is not done properly, will the > pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Child
RE: [Histonet] Stupid, stupid static!!
Hi Sarah, One of our staff uses the static spray now and then. It works pretty well but the "fragrance" isn't the greatest. We also have a couple of humdifiers in the lab. These seem to help as our air in central Oregon is a bit dry on this side of the Cascades. Don't know if dry air is an issue for you in Austin, but we love our humidifiers here. Actually one is this cool blue penguin that shoots mist out of his beak...makes me smile every day. Later, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjas...@copc.net 541/617-2831 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, January 05, 2011 11:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Locking up formalin
Hi Victoria, I've never heard of this, of course it could exist somewhere. I wonder what would prompt this. There are obvious safety issues to consider. Perhaps an unauthorized party, somewhere, got into some formalin and caused problems? Locked up or not, proper spill containment is mandated by OSHA (I believe) so, again perhaps an unauthorized personnel issue? I've worked in the Upper Midwest and Pacific Northwest and have not heard of this. I regularly attend the NSH and have not heard anything at the meetings either. Thomas Jasper Histology Supervisor Central Oregon Regional Path -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Spoon, Victoria Sent: Thursday, October 14, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Locking up formalin Is anyone aware of regulations stating that formalin has to be locked up- put in locked cabinets when not under direct supervision? Applying to either clinics where specimens are collected into formalin containers or in the pathology lab? Thank you Victoria Spoon Anatomic Pathology Manager Bassett Medical Center Cooperstown NY 13326 victoria.sp...@bassett.org Tel(607) 547-6357 Fax(607) 547-3203 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cutting, Processing, etc
Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the "enlightened" administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histot...@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my water
RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions?
Hi Tanisha, I think the question might be...who's requirements are you referring to. I see by the header you reference NY state. I see by your signature that you are in Indiana. I am not aware of any CAP reg referencing this and, to my knowledge most labs have their own job descriptions written with their own qualifications listed. You say "...right now I'm being told..." so again, by who (whom). You also reference a BA, I'm assuming this means BS or BAS works as well. I personally know very good supervisors that are associate degreed and hold HTs. I also know supervisors that have bachelor's degrees and I wouldn't necessarily say that they are any better because of it, however I think that's a "truism" across a lot of occupations. Just trying to understand your question. Have a good day. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha Sent: Friday, July 30, 2010 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 <http://www.covance.com/> - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
c Specificity >Accuracy on tests of known positive and negative controls >Controls of known concentration >Determine what could "Interfere" to confound the result > > >Diagnostic Specificity >Ability of a test to determine true diagnostic negative verses falsepositives (Higher % FP = less specific) >Requires comparison to a previously validated antibody > > >IHC Specificity >Ability of an antibody to bind exclusively to its particular antigenin the absence of staining of other molecules >Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-Original Message- >From: histonet-boun...@lists.utsouthwestern.edu >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >jmye...@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjas...@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >-- > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histo...@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Se
RE: [Histonet] New CAP question ANP.22760
Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpe...@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in &qu
[Histonet] FW: test
test _ From: Thomas Jasper Sent: Friday, June 18, 2010 11:17 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: test Thanks, checking on connection. tj ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Are Histotechs considered "exempt" employees?
-Original Message- From: Thomas Jasper Sent: Tuesday, May 11, 2010 3:45 AM To: 'Anthony Sandoval' Cc: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? Anthony, Don't know where you live in Cali or where you work. But if you are an HTL and have any decent skill/experience, I would think you are being totally ripped off. The cost of living in most parts of Cali alone makes me wonder about this salary. And when you ask about being exempt...I'm assuming you are exempt? If this is the case again a total rip off. I think you need to look for work elsewhere and check into wages. New students that are registry eligible start out much higher than that to my knowledge. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor CORPS Bend, Oregon -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered "exempt" employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] studying for ASCP certification exam
Jennifer, I think all the suggestions you've gotten so far are good. I'd add - The Theory and Practice of Histological Technique - by Bancroft and Stevens. I also believe there are NSH study materials available, maybe the BOR guide you mentioned is the same thing, not sure. Good luck! Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/617-2831 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, April 13, 2010 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] studying for ASCP certification exam I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying "Histotechnology: A Self-Instructional Text", by Carson and just recieved the "BOR study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
I'm inclined to agree with you Andrew. Seems to me that CAP has become, unintentionally (I'd like to believe) something of an unsavory, bullying sort of entity. I'm not certain about all the factors involved, but I think a few things have definitely contributed to all of this CAP negativity. First of all - CAP was (is?) considered the accrediting "gold standard". That's pretty heady stuff...possible ego inflation potential...high horse attitude...elitism? Not saying that was the plan, just an unfortunate and unintended consequence. I'm sensing a power trip here, it's partly human nature (I guess) and it can definitely suck! Secondly - as I recall, CAP got egg on their face a few years back in the DC/Baltimore area I believe. Someone can correct me if I'm wrong or provide more specific details. Anyway, the gist being - someplace passed their CAP inspection and someone (an employee I believe) contacted another regulatory agency because there was NO way this place should've passed CAP. When this all came to light CAP had to respond, and as can often be the case, the response was overly compensatory. I'm sure a lot of folks out there know what I mean. Now CAP issues confusing and sometimes unnecessary regulation changes and additions. And of course we're all aware of the "super secret surprise" inspections. I'm not even sure of half the other hidden agendas and possible ulterior motives. Control issues, tarnished pride, bruised egos and all conveniently cloaked in a drive for the best possible patient care...who could argue with that standard? During my previous employment, we "sweated" the details and worked diligently to achieve our 1st CAP accreditation circa 2000. I have to admit, I did/do like the regulation format. Having a question asked, determining if it applies to your service, and then answering yes or no. By taking it from there and doing things on the up and up, most any lab, that's honest and conscientious should have the realistic expectation of passing CAP. That was then, it seems to longer be that way. As I mentioned, confusing language and reg. additions/changes, along with CAP inspectors and their agendas have all been to the detriment of the accreditation. I was trained to look at CAP as "peer review". In my experience, many times this was not the case. Many CAP inspecting "teams" wants to make the "inspectees" (if that's a word) something of a clone, carbon copy or version of the inspecting team's service. This is another huge problem and causes a lot of strife, hard feelings and red tape at and after the summations. The regulations, ideally, should be interpreted in the most objective way possible. Again, maybe it's human nature, but it seems that people can't help being overly subjective re: interpretations of any number of CAP regs. I used to work with a pathologist that regularly attended the CAP committee meetings. At times I would bring issues to him I thought relevant to CAP. I don't recall the specifics but I do know they were of a practical nature from a technical viewpoint. I basically got the brush-off and was led to believe that CAP wasn't interested in the "technical" viewpoint and he wasn't going to bother with it. This may be a stretch in logic on my part...however, I can't help but think if CAP would listen to technical folks as well as MDs, they'd be in a better position right now. I'm not inclined to throw the baby out with the bath water. I think CAP accrediting was established with good intentions. Somehow things have gotten out of hand, and some have gone horribly wrong. I think things like QIP are good, although I've heard complaints about that as well. I'm feeling lucky these days because CAP isn't in my life. But my attitudes and mind-set have most certainly been shaped by my CAP experiences. Please remember this is my opinion only, I am not perfect and am only interested in practical application of sensible regulations for optimal patient care. Regards, Tom Jasper Histology Supv. CORPS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Thursday, April 01, 2010 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential "perception" that you are better covered from a legal standpoint, CAP certs are worthless.
[Histonet] California Histology Society
Hi There, Anyone out in histoland know why there's no access to www.californiahistology.org ? I've tried a few times this morning with no luck. I want to send one of my techs to the symposium and need to access the event site. Thanks in advance for any help or explanation. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Contamination..processor?
Hi Brandi, Don't know if I can solve your problem...but here's a few questions. 1)You've determined that the floater (tonsil cells in this case) are in the block. Did you determine this because you consistently see the same floater in the same spot, level after level, slide after slide? 2)Do you use a forceps warmer at your embedding station? If so, have the wells of that warmer been cleaned out lately? It can be a source of floaters. 3)Do you have 2 processors? If so, are you running separate programs (on separate machines of course) for large and small specimens? If you can do this and you are not, you might want to consider it. 4)While it's possible you could be picking something up from your processor, I would not be initially suspect of it. Are you running clean runs after processing runs? If you are this should basically take care of any residual tissue floaters that may have gotten out of a (tonsil) block, or any other block for that matter. You embedded the GI biopsies first, so I would not suspect the embedding center work surfaces to be a source of your tonsil floater. Some machines have little grooves to allow waste paraffin to drain off, sometimes things can be trapped there. Also, you say that the pathologist grossed the tonsils after the GI's. I believe you and him/her, but was anything else grossed before the GI's? Some type of lymphatic tissue? I tend to look to the grossing bench 1st for the source of floaters because once it's done there, it shows up everywhere else. Also, when techs cut and embed, they have no choice but to cut and embed whatever they're given. There is no way to determine if what you might be looking at is a floater. And more often than not, when cutting and embedding you've inherited a floater as opposed to introducing one. Good luck, hope this helps. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tigger...@aol.com Sent: Wednesday, March 24, 2010 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] GI, Uro, or Derm Path Lab Set Up
For all. tj -Original Message- From: Thomas Jasper Sent: Friday, February 05, 2010 1:34 PM To: 'Blazek, Linda' Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear Linda, I have received private responses similar to yours. Seeing that you've posted for everyone I am compelled to respond. Let me start out by saying my issue, complaint, concern - whatever you want to call it obviously is not aimed at an operation such as yours. As a matter of fact my service is a stand-alone Histo/Cyto practice. We are full service, accredited and located 2 blocks from our main med center. Our situation is like a path department in a hospital, minus the politics, etc. I'm saying this because I am not against, private, above board, accredited operations, which are run ethically and free of ulterior motives. I apologize if anyone took offense, but I think it should be clear if your not what I've described, then your not it. Secondly, it was not my intention to vent in an uneducated, juvenile and unprofessional manner. Quite the opposite as a matter of fact. Here's what I know about so called "pod" labs, which by the way does not lump everyone into one group. I interviewed a candidate 2 years ago that came from a "pod" lab. I was appalled as she described the working conditions, facilities, lack of equipment and support she received with this service. Improper ventilation and plumbing, inadequate space, under-qualified assistance, unreal expectations from those in charge. This was an unethical practice at best and I'm at a loss to understand how it was allowed to legally operate. Through professional contacts I've been made aware of more and more "fly-by-night" ventures which are based on a lucrative financial reward for a few at the top. This is at the expense of facility and technical support. Along with this comes patient care risk. Nine months ago I lost an excellent tech to a "pod" lab. Within 2 weeks he was calling and e-mailing back, regretful of ever getting involved with this operation. He was lied to, overworked, underpaid and totally mis-lead by a poorly conceived and financially unbalanced venture. Fortunately, he will be rejoining us next week and he basically cannot get away from these people quickly enough. Now in our area plans for a "pod" lab are in the works. This facility does not have the space, ventilation, plumbing or staff. The group behind this venture has a history of upsetting behavior in the medical community. Let's just say they're controversial at times. As I mentioned previously they've advertised for a tech but I have no idea how or what they think they're going to pull off. I'm sorry but there are a ton of red flags here. The only reason I can think of that these operations are allowed to exist is that they skirt regulations somehow. And this has been alluded to by others posting on this list. My point is that I personally cannot abide subpar operations, that exist only to line the pockets of unscrupulous parties. Which in turn potentially leaves patients and well meaning staff in it's wake. If this is not you, great, more power to you and carry on. If this is what you're involved in consider yourself put on notice and remember karma can be a real bitch. Hopefully clear and not misunderstood, Thomas Jasper -Original Message- From: Blazek, Linda [mailto:lbla...@digestivespecialists.com] Sent: Friday, February 05, 2010 12:33 PM To: Thomas Jasper; Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear all that are blatantly lumping all private or office specific GI, GU or Derm labs into one lump. It is very uneducated and juvenile to vent in such an unprofessional manner. You are not very well informed and/or ticked off at losing revenue from your hospital facility. Our facility complies with and exceeds all standards set by CAP, CLIA, OSHA and COLA. We are inspected at the required intervals by both COLA and CLIA. We do the required air quality validations. All of the staff is certified and participates in annual continuing education. Our main goal is patient care. Our pathologist interact with our clinicians on a regular basis. Our Quality Improvement, Quality Assessment and Quality Management is excellent. We have received the Laboratory Excellence Award from COLA and high praise by CLIA. All of our equipment is state of the art and regular maintenance is performed. There isn't a member of our team that would not either themselves or refer a member of their family to our facility. I don't think there is an employee here that wants to leave here and go back to the hospital facility and have to deal with the ever increasing drive to put out more and more work in less and less time. Our goal here is quality not quantity. Th
RE: [Histonet] GI, Uro, or Derm Path Lab Set Up
I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tja...@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/
RE: [Histonet] Staffing for IHC
I would totally agree Angela. Without knowing all the details, I'd bet these people are plenty busy all day everyday. I'd worry about burning them out. And what happens when one or the other is sick or needs a vacation? Tom J Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, December 17, 2009 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing for IHC I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] VENTANA AUTOSTAINERS PLS
One more time! -Original Message- From: Thomas Jasper Sent: Friday, December 11, 2009 3:24 PM To: 'Jimmy A' Subject: RE: [Histonet] VENTANA AUTOSTAINERS PLS Jimmy, What the...!!? I don't think anyone is going to be able to help you. This is a vague request and I'm beginning to wonder about the sincerity and credibility of it. My suggestion - Either get real or please get off this list and leave folks be. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jimmy A Sent: Friday, December 11, 2009 3:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA AUTOSTAINERS PLS Hi, I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer works. I am an histotech working here in the US. I am interested in learning the operation of the ventana immunostainers. I will appreciate it, if one of the histology/ihc labs could grant me this great favour. Hoping to hear from you guys asap. Jimmy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bachelor's Degrees
One more reason to consider "carefully" before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Hi Jeri, He should be fine. Passing the HTL demonstrates considerable knowledge which is applicable to the clinical (human) lab world. Also, I'm of the opinion that animal tissue - in general - is more difficult to section than human tissue. Not actually knowing the person - which makes a difference - and with the information you've provided, again, he should be fine. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of j...@opssearchgroup.com Sent: Thursday, December 03, 2009 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Histonetters, I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] a basic question about immunohistochemistry
Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syi...@ucalgary.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Floors
My thanks to everyone for their input about cleaning lab floors. Much appreciated. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Floaters in Waterbath
One more time. tj -Original Message- From: Thomas Jasper Sent: Friday, October 23, 2009 10:41 AM To: 'Stella Mireles' Subject: RE: [Histonet] Floaters in Waterbath Others may mention this to you...and it can get a little political. Do not discount the grossing bench. Whether it's the work of a PA, Pathologist, or other qualified lab staff, the grossing bench should be kept as clean as possible between cases/specimens. I mention the political side because sometimes it gets a bit touchy...histologists may not be in the best position to broach the subject with certain higher level personnel. Especially when the grossing may very well be done under the supervision of said higher level party. >From a patient care standpoint, etc., this definitely should not be an ego-bruiser, as we are all human and make mistakes. But I'm sure most of you know what I'm talking about and probably have experienced something similar at sometime in your careers. One tip I learned from a pathologist, was to keep a clean sponge handy while grossing. This helped a lot, especially with keeping forceps etc., clean in between cases/specimens. Lastly, there are pathologists and PAs out there that keep their egos in check and we are thankful to them. Tom J. Thomas Jasper HT (ASCP) BAS Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 7:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Inspection question
The reason for that being? Pray tell... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 22, 2009 9:08 AM To: histonet; Patti Loykasek Subject: Re: [Histonet] Inspection question Yes! René J. --- On Wed, 10/21/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Inspection question To: "histonet" Date: Wednesday, October 21, 2009, 6:15 PM Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Inspection question
Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Floor Cleaning
Hi Folks, Got a floor cleaning question for you. We are transitioning to a new floor cleaning crew in our histo lab. I've been asked by the building manager to elicit some opinions about products to use and/or techniques to best get paraffin up off of linoleum. We were fine with our previous cleaners, however, the thinking around here is maybe it could be done better? Is a machine required or not? Could we do it at a lesser cost, and in a more bio-friendly way? Is there an easier and simpler method? Anyway this certainly is not the hot issue of the day for Histo-net. However if anyone would care to share anything on this it would be appreciated. Thanks, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] MUM 1 antibody
Hi Martha, We run MUM 1. We get it from Cell Marque and run it on our Ventana Benchmark XT. We use a tonsil control and our hemepath likes it just fine. I realize you're running the Bond. Don't know the particulars about protocols for the Bond. We incubate for 32 minutes at 37 degrees (if that helps). You could start there anyway. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, September 29, 2009 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM 1 antibody I have been asked by my hematopathologist to work up MUM 1 antibody. Does anyone have any suggestions as far as vendor? Any additional advice for the Bond stainer would also be appreciated. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org <mailto:sfe...@cmc-nh.org> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] OSCAR Antibody
Hi Maria, We run OSCAR on Ventana Ultraview - CC1 Mild, 37 degrees C, 16 minute incubation. We get the antibody from Covance - product #SIG 3465-16. This comes as a 6ml predilute. Our docs like it quite a bit, and order it on a regular basis. Hope this helps. Regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Wednesday, July 08, 2009 1:58 PM To: Histonet Subject: [Histonet] OSCAR Antibody Can anyone tell me where I can get a really good OSCAR antibody that works with Ventana Ultraview detection kits? I need a pre-dilute if possible. Thanks, Maria Katleba HT(ASCP) MS Pathology Dept. Mgr Queen of the Valley Medical Center Napa CA 94558 707-257-4076 Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologist Position in Bend, Oregon
Fellow Histonetters, A position has opened for a Histologist here in beautiful Bend, Oregon... Central Oregon Regional Pathology Services - Bend, Oregon seeks a full-time Histologist, HT, HTL or registry eligible preferred. Responsibilities include - Embedding, Microtomy, Routine Staining, Automated Special Staining and Immunohistochemistry. Excellent benefits, including health insurance, retirement plan and competitive salary. Potential sign-on bonus as well. Bend is located in central Oregon at the base of the Cascade Mountains. World class outdoor activities abound, including biking, hiking, rock climbing, fishing and camping. Phenomenal alpine and nordic skiing available October through May. Bend is uniquely situated with the Cascades to the west and the high desert to the east. Although in the Pacific Northwest, Bend enjoys warm, clear days and cool evenings most of the year. Winters are mild with a clean environment and accessible wilderness. In about 30 minutes you can reach the scenic beauty of canyons and rock formations in the high desert. Crater Lake national park is approximately 100 miles south and the Pacific coast can be reached in just over 3 hours. The lush Willamette Valley lies between the Cascades and the coast and is stunningly beautiful as well. Many wonderful restaurants of varied cuisine are located all over town, outstanding wine is produced locally and 4 microbreweries produce top notch beer. Bend has a community symphony orchestra, a diverse musical scene and many art and cultural events. The Univ. of Oregon and Oregon State Univ. are in close proximity, and Portland is only a few hours away. We are a progressive, friendly lab and a great place to live. Contact us at - Central Oregon Regional Pathology Services 1348 NE Cushing Dr. Bend, OR 97701 Attn. Pam Sylvester or fax resume to (541)693-2648 or, you may contact me as well, Tom Jasper at (541)693-2677 Thank you, Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjas...@copc.net 541/693-2677 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disposal of Formaldehyde
You are correct Nanette. Waste water treatment is under the jurisdiction of local regulations from place to place. What's allowed in one place may not be in another. Seems it depends on what various waste treatment facilities have the ability to handle. It also seems, historically what has happened to water in a certain regions and what folks will tolerate. I'm not trying to excuse pollution, I'm just stating what I understand exists. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 10:24 AM To: 'rjbu...@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. René J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako
OK, There've been plenty of shots taken at Dako...and from what I can tell rightly so. I agree with the statements about the "good old days" and all, and I would still like to believe Dako is a decent antibody company. So here's my question - How 'bout it Dako? Are you going to take all of this lying down? Do you have any response(s) at all? You (collectively as a company) probably owe a lot of these folks some sort of explanation. I find it hard to believe that Dako has opted to adopt a business model, which (from all appearances) has taken a path of self-destruction. And I don't care who the parent company is now (Danaher?) these anecdotes exemplify irresponsible handling of business. It certainly isn't necessary to have attended Harvard (insert your favorite) business school to draw this conclusion. I also believe that competition is good for the marketplace. By having Dako, a one-time leader in the field, basically "tank" isn't good for anyone. I'd like to see something salvaged here, but it's not up to me. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question of the day - embedding
Tracy, I used to work at the same (nameless) place with Mr. Saby and I concur. In my opinion your colleagues are potentially causing harm to the tissue specimens (especially small, delicate ones) while you are not. Hot paraffin, specimens of various tissue types (particularly animal) and the resultant heat transfer is less than optimal. Consider...why is it so important to get your specimens off the processor(s) shortly after the run(s) have been completed? Answer...you don't want to "cook" them in liquid paraffin on the final station. So why cook them in a holding tank full of liquid paraffin while you embed? Also, the tissues (ideally) have been properly fixed, processed and infiltrated. No harm will befall them without liquid paraffin, until they are embedded. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Tuesday, February 17, 2009 3:04 PM To: Tracy Bergeron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question of the day - embedding Tracy- Where I used to work (at a place that shall remain nameless), we always kept our tissue being embedded in hot paraffin in the holding chamber. Most of my work has been with animal tissues. Where I work now, we don't. And I do bellieve you are right. If the tissues remain in hot paraffin, the heat transfer rate is very high, and the tissues continue to "cook" even when the chamber temperature has been reduced (as close as feasible) to the melting point of the paraffin. I have seen little effect on the tissues of longer-than-I-would-like time in the holding chamber without paraffin. Without the paraffin, the tissues do not get that direct heat from the melted paraffin and survive delay much better. In short, I agree with you. Not keeping the tissue in hot paraffin does not only not damage those tissues, it allows more flexiblity in your embedding times. Joe Saby, BA HT From: Tracy Bergeron To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 17, 2009 4:14:46 PM Subject: [Histonet] question of the day - embedding Hi all question/dilemma of the day. I have been of the view that the longer tissue sat in melted paraffin the harder it got, especially animal tissue. So with that said, for the past nearly 10 years I have not used melted paraffin in the holding chamber of the embedding center. I just keep the chamber warm, and work that way. Thus keeping the tissue from continuing to cook and harden in the wax. Everyone else I am currently working with has never seen the method I use, and firmly believe that this causes harm to the tissues if they are not in paraffin. Thoughts ideas etc. I am dying to know if I am the only one that worries about length of time that animal tissue sits in paraffin. Thanks. Sincerely, Tracy E. Bergeron, B.S., HT, HTL (ASCP) Associate Scientist III, Pathology Comparative Pathology Laboratory Biogen Idec 14 Cambridge Center Cambridge, MA 02142 Direct: 617-914-1115 Fax: 617-679-3208 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem with Schiff's reagent
Hi Paul, Some people may think this is taking the "easy" way out, but...you might consider purchasing some commercially prepared Schiff's. It will (should) be consistent product (consistently produced) and the manufacturer has the responsibility of QC'ing the product before it is shipped and sold. There are any number of reliable companies handling Schiff's (I would not favor one over another). You could type "Schiff's" into your search engine and I'm certain you'd be able to contact a supplier. This may be an answer for you. Good Luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pbru...@siue.edu Sent: Saturday, February 14, 2009 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with Schiff's reagent Good Morning! I'm a newcomer to the list, and I'll start by pointing out that I'm not a histologist by any means. I am a freshwater ecologist, and we're trying to study mucus secretion behavior in freshwater snails. Last Spring and last summer, we developed a process whereby we could visualize snail mucus trails on glass slides using a periodic acid-Schiff's reagent staining technique. But now, in following the same protocol as used last year for making our own Schiff's reagent, I cannot get the final solution to filter out clear. Recipe I'm using: 900 ml boiling water 10 grams basic fuchsin 25 ml concentrated HCl acid (12 M) 40 grams sodium metabisulfite (this is essentially Sigma Aldrich's ratios, I think) Let this sit for 24 to 72 hours, take 100 ml aliquot, add 0.75 - 1.0 gram ground activated charcoal, stir for 10 minutes, filter through filter paper then through GF/C glass fiber filter. Last summer I got nice, clear (slightly yellow) and very active Schiff's reagent. But now I cannot seem to get the filtrate to be clear. Even after 10 minutes exposure to ground activated charcoal and filtering, the filtrate remains bright orange to dark red and it does not seem to stain mucus trails very well. All the reagents are the same as those used last summer (i.e., less than 7 months old; although the HCl is a bottle several years old from a different lab). Anyone have any troubleshooting suggestions? I don't know the chemistry very well, but the sodium metabisulfite is used for "decoloring" the initial solution, right? So is the metabisulfite not working for some reason now?? Any help/suggestions would be greatly appreciated. Cheers - Paul Paul E. Brunkow, PhD Department of Biological Sciences Southern Illinois University Edwardsville Edwardsville, ILUSA - SIUE Web Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] re: Certified Histotechs
Thank you Jennifer, well stated. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, February 12, 2009 9:16 AM To: christopher.conl...@kp.org Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] re: Certified Histotechs I would like too clarify the role of NAACLS in training histotechs for certification. There are a number of different NAACLS accredited programs in the US. All of these programs meet the standards set by NAACLS or they do not remain accredited. Providing on the job training (OJT) is NOT one of the requirements. All of the programs have a practical component to them. The hospital-based programs will provide the practical instruction as well as the didactic portion. College based programs will provide the didactic portion and some also provide some practical portions and the student will be placed in a clinical affiliate work site for further practical training. Other college based programs will place the students into internships for the practical training after they complete the didactic portion, or a variation of this. One of the challenges that NAACLS accredited programs have is to get clinical affiliate sites to place their students. There are affiliation agreements, forms, and certain standards that these sites must meet. Another challenge is to get comparable training between sites. Not everyone out there is willing to be a part of the training of these students. There are some employees that refuse to help train the students or even let them observe them working. The ASCP does not "frown" upon OJT people. Route 2 (AS or equivalent {with sciences} plus 1 year of histology work experience ) allows for the work experience component for certification eligibility. The ASCP, upon much research and feedback from the "professional world" has established a minimum education requirement., Applicants that applied for certification under the old route 3 (high school and 2 years of histology work experience) had an HTexam pass success rate around 30% on the computer portion. Many of those applicants did pass the practical portion. It was determined that in many cases a high school education does not provide the foundation for the theory portion of the exam. The quality of OJT techs is not being called into question. The histology community has for many years demanded respect and higher wages to rival nursing and medical technologists. We are the only clinical profession that does not require some form of certification or license. In order to command the respect that the profession deserves we have to set standards. Certification is a way to set the minimum standard. Is it perfect, no. There will always be people that can test well and perform badly, and visa versa. That folks is life. For those that are working in the lab now and are not certified there are a couple of NAACLS accredited on-line programs to qualify for route 1. You can take college credit courses to work toward your degree to qualify for route 2. Encouraging high school students into our profession is great, but encouraging them to forgo college to do it is a disservice. The world of histology has changed a great deal over the years. There is more of a demand for higher complexity testing that did not exist when I first trained. Someone mentioned that automation is taking over. You still need people to trouble shoot and QC. What happens when there are problems? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu christopher.conl...@kp.org Sent by: histonet-boun...@lists.utsouthwestern.edu 02/12/2009 06:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] re: Certified Histotechs I find it smartly ironic that all these "Lab Managers", MBA's," Med Techs", are so offended and defensive in regards to BOR(ASCP), for histology? Why? There are journeymen electricians. Many technical fields have registration/certification. I worked for 10 years as a Phlebotomist, a Deiner, a lab assistant, and I didn't get paid squat until I went to I.U.P.U.I., graduated on the Deans list and then sat for the exam (clinical and practical), passed the exam etc. Part of the reason pathologists and clinical lab scientists and MBA managers are so condescending about histology, is because, they want it to always be an easy access career, and the medical field is like the animal kingdom or a caste system, it has its own little system of who is who and how dare you ask me that I am a (you fill in the blank). Also, they ca
RE: [Histonet] uncertified techs
Steve, You never needed to pay the $45 a year to the ASCP. Once you were certified, you were certified. No one can take that away from you. And $45 for a sticker wasn't insurance of anything. That money went to supporting the ASCP and getting you a copy of their magazine. I realize it's different today for new certificants with the CE requirement, which is actually not a bad idea. To everyone else interested in this discussion, I fall on the side of ASCP certification being the unifying factor. And I've still not been convinced that state licensure (or whatever kind of licensure) is necessary. We have a national standard with the ASCP-BOR, I see no need to re-invent the wheel here. And I realize I'm probably treading ground somewhere here between the advocates for licensure and those who feel uncertified techs are good enough and if you have more training, education, etc., that's nice but not required. Seems to me that whatever the field of endeavor, you will always find people doing exceptional work and people without a clue. To paraphrase George Carlin, "Somewhere out there is the world's worst doctor, that's bad enough, but what's worse is that someone probably has an appointment to see him today!" So, this may or may not have anything to do with degrees, certifications, licenses or special training. I think the general public would like to believe it does, or why would people bother to frame all their diplomas, certificates etc., and put them on the walls of their offices? It does seem logical that someone with more training, education and all would be a better tech. The reality is sometimes that's true and sometimes it's not. I like the idea of a well educated and technically skill person working in my lab, but everyone brings something different to the table. Also, from what's been posted already it's obvious to me that life circumstances have dictated how things shook out for most people. Everyone wants respect no matter which path brought them to the lab. I think we can agree on 2 things here which are both less than optimal - 1)Having a technically skilled person that's good at embedding, cutting and staining, but doesn't have a clue about chemistry, biology or much in the way of science -that's a problem! 2)Having a certified, bona fide, glorified (and possibly funk-defied)degree holding, so called, well educated person that can't walk and chew gum, let alone get any lab work done -that's a problem! I don't have the answer, but I do know that having the state hit you up every year for $$??, just to keep a license seems wrong! In the last year, I've hired 3 well educated techs, that will eventually take their registry exam. I appreciate the education they've got. The level of technical expertise I get from them is good to very good as well. So, I'm lucky in that regard. I do believe it was a mistake to eliminate the practical portion of the exam. I can elaborate on that with anyone who disagrees if they wish, but this post is probably long enough for now. Also, I'm sure this discussion can seem confusing and odd to our UK, etc., colleagues, but their social medicine makes for a completely different beast. Thanks for allowing me to ramble. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/617-2831 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Wednesday, February 11, 2009 9:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] uncertified techs Thanks for all the input. So why am I wasting my cash paying the $45 for an ascp sticker? 15 years ago I suppose I should have taken the extra time to become at least an MLT. Oh well. Thanks again ya all. :) Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue swelling out of block
Hi Gudrun, Is your friend soaking blocks in ice water before cutting? This is common in animal histology and over soaking before cutting will cause swelling. Different tissue types will swell more quickly than others, but over soaking can eventually affect all tissue. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels in the lab
Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet