[Histonet] Question about 72 hour upper fixation time for HER2
Hi Histonet, Does anyone know if the 72 hour upper fixation time for HER2 was calculated at room temperature? My lab is currently running our formalin at 40°c and we go 72 hours on the long weekends, so I'm worried it might be overfixed. Thanks, Tony -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [External] Tissue processor advice
I went through 2 ASP300, The first model not the S and they are terrible! I had a sister lab that got a new/used ASP300S and the hard drive died within a couple months. I would really recommend against them. The only Leica certified repair people in the Phoenix area would not work on my ASP300 because they break so often and they didn't want to be responsible for it. On Wed, Sep 16, 2020 at 11:52 AM Daniel Pesino via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Colleen - > > I've used both and I enjoyed both machines. The Leica instrument feels a > bit more modern and the reagent management system is useful for reagent > changing schedules. The changing of reagents and wax is pretty easy with > the drainage hose. I've rarely had any issues with them, but have had > plenty of issues with the Peloris instruments (made by Leica). > > The VIP is a workhorse. Very rarely have we had problems with the > instrument. It's very reliable, but not quite as "flashy" as the Leica > instrument. > > I'm not sure about parts for the VIP, but if you don't have a service plan > for the ASP300S, be prepared to pay out the nose for parts. I was quoted > over $1,000 for a wax drain hose that was literally a piece of plastic > piping with a simple connector on it. > > Best, > > Dan > > Daniel Pesino, HTL(ASCP)QIHCCM > Senior Histotechnologist > Trinity Health Of New England > daniel.pes...@trinityhealthofne.org > W 860-714-4675 > > 114 Woodland Street > Hartford, CT 06105 > TrinityHealthOfNE.org | Facebook | Twitter | Instagram > > > > > > -Original Message- > From: Colleen Forster via Histonet > Sent: Wednesday, September 16, 2020 1:17 PM > To: histonet-request > Subject: [External] [Histonet] Tissue processor advice > > Warning: This email originated from the Internet! > DO NOT CLICK links if the sender is unknown, and NEVER provide your > password. > > HEllo Histoneters, > > I am looking at replacing my VIP2000, porr girl finally quit on me. > > The two processors I am looking at: > > Leica ASP300S > Sakura VIP5 > > Any of you out there who have used either of these or both, can you give > me pros , cons, yes, noI am just looking for experiences those who have > used them can share. > > Thank you in advance. > > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 612-626-1930 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing Schedule- ASP-6025
; > > > This has led us to question our processing schedule. I am not concerned > > > with our fixation because we fix everything for at least 24 hours in > 10% > > > formalin (commercially prepared) prior to processing. > > > > > > Does anything jump out at you as being a potential red flag in the > > > following overnight protocol? > > > > > > - Formalin 15 mins; RT > > > - Processing water 1 min; RT > > > - ETOH 70% 30 mins; 35C > > > - ETOH 80% 30 mins; 35C > > > - ETOH 95% 30 mins; 35C > > > - ETOH 100% 30 mins; 35C > > > - ETOH 100% 40 mins; 35C > > > - ETOH 100% 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Paraffin 60 mins; 57C; vacuum > > > - Paraffin 60 mins; 57C; vacuum > > > - Paraffin 60 mins; 57C; vacuum > > > > > > Our formalin is changed after every 1100 cassettes and the alcohol, > > > xylenes and paraffins are managed similarly by the instrument. Our > > specimen > > > mix is a little of everything (skins, GIs, breasts, needle cores, gall > > > bladders, gyne, etc). > > > > > > The one unknown (so far) in this story, is how the tonsil from the > other > > > laboratory was handled (ie the fixative used and for how long-I am > > assuming > > > 10% formalin). > > > > > > Obviously, many of you will have schedules that differ from this one, > in > > > any number of ways, but what I am looking for from you is your opinion: > > *is > > > there anything about this schedule that is particularly concerning?* > > Thank > > > you, Greg > > > > > > > > > -- > > > *Greg Dobbin* > > > 1205 Pleasant Grove Rd > > > < > https://maps.google.com/?q=1205+Pleasant+Grove+Rd&entry=gmail&source=g> > > > RR#2 York, > > > PE C0A 1P0 > > > > > > > > > *Everything in moderation...even moderation itself**!* > > > ___ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > This message is intended for the addressee named and may contain > > > confidential information. If you are not the intended recipient, please > > > delete it and notify the sender. > > > > > > Views expressed in this message are those of the individual sender, and > > > are not necessarily the views of NSW Health or any of its entities. > > > > > -- > > *Greg Dobbin* > > 1205 Pleasant Grove Rd > > RR#2 York, > > PE C0A 1P0 > > > > > > *Everything in moderation...even moderation itself**!* > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 10% NBF Neutralization - Aldehyde test
Hi Brad, I used the Neutrasafe Formzero Test Kit from statlab at my last job. It worked well. Happy Holidays, Tony On Thu, Dec 20, 2018 at 8:40 AM Ringer, Bradley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Does anyone currently neutralize formalin to dump down the sanitary system > drain? We are currently using Formalex Green to neutralize our formalin for > dumping. We have run into the issue of not being able to purchase aldehyde > testing kits to verify the formalin has actually been neutralized before > dumping. We have contacted the company, that in the past has sold us these > aldehyde testing kits, and we were told they no longer sell them because > histology labs are only checking the pH of the "neutralized formalin". > After going back and forth a few times, the sales rep now tells us that we > also need to be checking the aldehyde levels before dumping which is > exactly what we have been trying to tell him. So, if anyone is > neutralizing formalin to dump down the drain, are you checking the aldehyde > levels and if so what product/test are you using? > > Thank you and Happy Holidays, > > Brad > > Brad Ringer, HTL (ASCP) > Springfield Clinic Histology Manager > 1025 S. 6th Street, Springfield, IL 62703 > (217)528-7541 ext. 14078 > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Molecular Biology, MB(ASCP) study materials.
Hi Histonetters, I am interested in taking the the Molecular Biologist certification through ASCP. Can anyone recommend some pertinent studying materials for this exam please? Thanks, Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Cassette Labeler
I also have a Primera slide printer. It worked well at first but now it is 2 years old and prints very slowly. I can hand write up slides faster than they print. I will be in the market for a new slide printer soon and will not be going back to Primera. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Embedding
If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue falling off positive slides
Increase the time and/or temperature. I bake mine at 85 Celsius for 20 minutes on non charged slides. Section thickness is also a factor as well as the pH of your blueing reagent. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome
Of the three microtomes that you listed I would highly recommend the Thermo Shandon Finesse. It's of superior quality than the Microms I have used. Cutting is excellent and the fly wheel action is light yet fluid. You do have to oil it manually once a month but it's easy and you can clean it out in the process and keep it in good operating condition. I haven't used the Titan 5000 but I have used slides from Tanner that were of poor quality and I ended up returning them. I'm cutting on a used Leica 2125 right now. It is functional but it does skip when facing and block orientation is terrible. Getting used lab equipment is always a gamble so make sure your supplier is pleasant to deal with and offers a warranty. Not sure what your budget is but a new Finesse isn't too expensive for a new microtome and I'm sure you will be very happy with it. Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Employee Evaluation
Hello Histonet, I am a relatively new supervisor and have been tasked with conducting my first employee evaluation. Does anyone have any documentation or checklists they would like to share with me to help me get started please? Thanks, Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Best Study material for HTL
This study guide is pretty handy: http://www.amazon.com/Practice-Questions-Histotechnology-Examinations-Registry/dp/089189473X/ref=sr_1_1?ie=UTF8&qid=1393865047&sr=8-1&keywords=histotechnology+bor I used that and Carson to study for my HTL. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Equipment recommendations, please
The Thermo Shandon Finesse is my favorite microtome. Wish I had one at my lab... I've never really had a preference for embedding consoles. As long as it's ambidextrous it's fine with me, I'm a lefty. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] p16
I have had excellent results with P16 (JC8) from Santa Cruz Biotech. http://www.scbt.com/datasheet-56330-p16-jc8-antibody.html Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: wax on the floors
Goo-gone with a green scrubbie on the tough spots. Then alcohol to clean up the goo-gone. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Microtomes
Thermo Shandon Finesse is my favorite. Manual version requires oiling which is easy and gives you a good reason to keep it clean inside. I got one for about 6,000. The high end automatic version is the Finesse ME+ and is the best microtome I have ever seen. Someone that has never cut before could sit down and get perfect sections on their first block. The quote i got for that was about 12,000 which was too much for my lab. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cell Block Preparation
We spin the sample down, pour off supernatant. Resuspend in 5 drops thromboplastin and 4 drops human serum we receive from our phlebotomy lab. Let firm for 5 minutes and then fix in NBF. The agar suggestion probably works similarly if you can't get serum. On Sep 6, 2013 9:39 AM, "Dessoye, Michael J" < mjdess...@commonwealthhealth.net> wrote: > We have had mixed results in the past with cell blocks, but currently we > are filtering the fluid through a biopsy bag and processing that. We > seem to be retaining more specimen with that method (we have used > sponges and lens paper in the past). > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -Original Message- > From: Ann Specian [mailto:thisis...@aol.com] > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We > currently spin, pour off the supernatant, retrieve the sediment and > process in lens paper. > > Does anyone have a more current technique which renders better > cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, > a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Positive tissue controls for MSI antibody panel
Ok, thanks anyway. I tried Cell Marque, they just have normal colon to use for control tissue and they are $20 a slide. I am looking for tissue that has microsatilite instability for MSH-2 and MSH-6. On Mon, Aug 19, 2013 at 10:55 AM, Sebree Linda A wrote: > Question everyone: > > What are you all using for your positive tissue control when running the > MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2? We're contemplating > using normal colon that all four antibodies should stain but do we also > need to show that colon having the mutation does not stain for each > antibody as well to show there are no false positive results? > > The former would be the easy way out but I'm willing to construct a > multi-tissue block containing normal colon and colon with the mutation(s). > > Thanks for all your responses, > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Positive tissue controls for MSI antibody panel
Do you have positive tissue for MSH-2 and MSH-6? I am trying to validate my MMR antibodies but have been unable to find tissue that is positive for these two antibodies. Is there anybody that can sell me some control slides please? Thanks, Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com On Mon, Aug 19, 2013 at 1:03 PM, joelle weaver wrote: > normal colon, CRC, colon AC, another primary > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: lseb...@uwhealth.org > > To: Histonet@lists.utsouthwestern.edu > > Date: Mon, 19 Aug 2013 17:55:40 + > > CC: > > Subject: [Histonet] Positive tissue controls for MSI antibody panel > > > > Question everyone: > > > > What are you all using for your positive tissue control when running the > MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2? We're contemplating > using normal colon that all four antibodies should stain but do we also > need to show that colon having the mutation does not stain for each > antibody as well to show there are no false positive results? > > > > The former would be the easy way out but I'm willing to construct a > multi-tissue block containing normal colon and colon with the mutation(s). > > > > Thanks for all your responses, > > > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics > > IHC/ISH Laboratory > > 600 Highland Ave. > > Madison, WI 53792 > > (608)265-6596 > > FAX: (608)262-7174 > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopropyl Alcohol in the histology lab
I recently switched to isopropanol in my lab. One thing I did first, that you may want to consider, was to check with the manufacturers of my processors and recyclers that isopropanaol would be compatible. 1. Can't help you with this one 2. I did have to adjust the staining times for eosin and the dehydration after. 3. I have not had any IHC staining issues since switching. 4. I still use reagent alcohol on my Artisan special stainer. DAKO said it would not be compatible. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com On Mon, Aug 12, 2013 at 2:18 PM, Teri Johnson wrote: > Dear colleagues, > > I am entertaining the notion of switching over to isopropyl alcohol (IPA) > for our tissue processing and have a few questions. I know the xylene-free > processing and microwave processing protocols use this universally, but at > this time I am only interested in getting rid of the > ethanol/methanol/isopropyl alcohol blend. > > 1 - Are there any known issues with holding tissues in 70% IPA rather than > 70% ethanol/reagent alcohol? > 2 - Is there any impact on the stain line if you use it for H&Es and > hydration/dehydration of histochemically stained slides? > 3 - Have any of you who use IPA found any impact on immunoreactivity when > compared to using other dehydrants? > 4 - Is there anything else I have overlooked? > > We have pure ethanol available to us if needed for particular stain > applications. > > Best wishes, and thanks in advance. > > Teri Johnson > Manager, Histology > GNF - San Diego, CA > 858-332-4752 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing Fumes
I have the same processor. If you remove the back panel there is a drip jar for waste just inside. It might be getting full. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com On Fri, Aug 2, 2013 at 4:58 AM, MaryK Mendell wrote: > I run a very small Derm lab and starting having a new problem processing > and cleaning cycle. Nothing has changed processing wise but it seems we > have more fumes than before. I have the fume hood running plus a Aller > Air 5000 Voc D portable air purifer, all filters have been changed. There > seems to be more fumes while the processor is running and durining the > clean cycle. I'm at a loss here as what it could be. The processor is a > Tissue Tek VIP 2000 and I can't see any leaks any where and no error codes > present. I have called our HVAC guy to have him look at venting to see if > an issue, but with the hood check showing no blockage not sure it that is > the issue. Any suggestions? > > > > > Kate Mendell > Histopathology/Lab Manager > > Anyone who stops learning is old, whether at twenty or eighty. ~Henry Ford > > HOWARD S. GOLDBERG, M.D., INC > 990 Paradise Road > Swampscott, MA 01907 > TEL: 781.595.0151 > FAX: 781.592.6780 > kmend...@goldbergmd.net<mailto:kmend...@goldbergmd.net> > www.cosmesticdermcenter.com<http://www.cosmesticdermcenter.com/> > PRIVACY NOTICE: This e-mail message may contain confidential patient or > other information belonging to the sender that is legally privileged. This > information is intended only for the use of the individual or authorized > entity named above. The authorized recipient of this patient or other > confidential information is prohibited from disclosing the information to > any other party. If you have received this message in error, please notify > the sender immediately and delete. Please keep any information you may > have viewed confidential. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Losing tissue on IHC slides
How are you drying your slides? If in an oven, what's the temp and duration? Try going longer and tap off excess water before drying. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sections
We cut the way your Pathologist is requesting at my lab, it's simpler and faster then the way you are currently cutting. I was used to cutting the way you described but this way is much easier. Check with your other Pathologists that they will be ok with the switch. I have certain Pathologist that will order more deepers because they want to see more of the block. It will speed up cutting in the first place but may take more time to cut deepers later in the day. -- Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome advise...
The Thermo Shandon Finesse ME+ is my all time favorite =) Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I recently was having salt percipitation problems with my VIP proccesor and we were doing a hot water flush every week. We were starting with 90% alcohol and heat on the first station and also using 10% NBF. We switched to 50% in the first station and 70% in the second station. This has not only got rid of the salt problem but our small tissue biopsies look much better. I'm not sure what your technicians concerns are about but from my experience lowering the concentration of the first alcohols is more gentle for the smaller tissues but it might not processes big fatty specimens as well. There will be also more reagent carryover from the water introduced in the first station but is worth it in my opinion. Good Luck! Tony Auge HTL QIHC (ASCP) Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MSH2 and MSH6 validation
Hello, I am looking to incorporate the MSI IHC panel into my lab and need to validate the antibodies. I have positive tissue for MLH-1 and PMS2 and have validated the performance of those antibodies but I can't find any positive tissue for MSH2 and MSH6. Would anyone be willing to help me out with where I might be able to procure some positive tissue? Thank you, -- Tony Auge HTL (ASCP) Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
