Re: [Histonet] ST4020 Stainer (Ingles Claire)
We like using Richard Allen products. Eosin Y Alcoholic, hematoxylin 2 and their clarifier On Sat, Feb 16, 2019 at 10:04 AM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. ST4020 Stainer (Ingles Claire) > > > -- > > Message: 1 > Date: Fri, 15 Feb 2019 21:24:01 + > From: Ingles Claire > To: Histonet > Subject: [Histonet] ST4020 Stainer > Message-ID: > < > dm5pr17mb124139962667a811d3577ab7c9...@dm5pr17mb1241.namprd17.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > Netters! > A question on behalf of a co-worker. We are in the process of validating > the ST4020 stainer from Leica. We are a Mohs clinic that currently uses > Carazzi's double strength Hematoxylin. We are looking for a better staining > protocol. We are planning on moving to a Harris formula when switching so > any Heme formula is possible right now. > Claire > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 183, Issue 13 > * > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidity issues-desperate for help
Hello Histonetters! I am currently working in a Mohs lab in Minnesota and during the winter time we have no issues with humidity but comes summer, my slides get washed out by the end of the day. I have tried everything I can think of but NOTHING works. I have tried changing solutions throughout the day, keeping the stainer lid open, adding molecular sieve in my alcohols and xylene substitutes, and a dehumidifier. We have the mini Thermo linear stainer under an airfiltronix filter which adds to the humidity issue. If anybody out there has other solutions I haven't tried yet please send them my way! One more thing that I have heard a long time ago at a conference is that if you use xylene substitutes then you do not have to use a hood/any type of ventilation system. Does that sound correct to anyone? Is that statement true? I have worked in labs that coverslip straight from alcohol and a ventilation system is not required but I notice the staining is not optimal in the long run. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MITF for Mohs frozen sections
Hello all, Mohs surgeon would like to start doing MITF (microphthalmia transcription factor) for melanoma with a chromogen yielding red results manually. I have never done them manually and do not know where to begin. We have a bond max but it keeps breaking down and you can't stain manually since the AEC and DAB are mixed by the stainer itself. Please help with any information and or procedure/protocols you have. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening- Dana Point, CA
Prominent, upscale, three physician dermatology practice seeking a full time (Mon-Fri) histotechnician with Mohs experience for Dermatology clinic located in Dana Point, CA. The technician will assist in Mohs tissue processing once a week and paraffin encompassed tasks on all other days. *Responsibilities include:* · Mohs frozen section preparation Mapping, inking, embedding, sectioning, staining and manual coverslipping · Paraffin section preparation Grossing, processing, embedding, sectioning, staining and manual coverslipping · Maintain Mohs and biopsy logbooks Record keeping for blocks and slides sent out for consultation or special stains. Filing slides and blocks as necessary. · Maintain equipment and comply with CLIA regulations Perform maintenance on equipment as needed and troubleshoot when needed. Record on logs all maintenance and daily usage. *Experience/Qualifications:* · At least one (1) year of experience in a technical role in a histology lab and Mohs surgery · Excellent organization skills · Ability to perform tasks and meet deadlines unsupervised *Preferred but not required:* · HT certification · Prior experience in dermatology *Generous benefits package includes: Medical and vision insurance and 401K, excellent work environment* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
