RE: [Histonet] What is a great manual mirotome
We use the Leica RM2255, RM2235, RM2165 and RM2265 and we love all of them! We are a research lab and are cutting paraffin and plastic. What are the specific issues that you are having with them? Nikki Nikki M Wahlberg Histology Lab Supervisor Preclinical Sciences Boston Scientific 763-694-5739 wahlb...@bsci.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 12, 2011 1:36 PM To: 'lsc...@sfcn.org'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What is a great manual mirotome I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MMA Formulation for EXAKT Grinding
Hello Everyone, I was wondering if you could help me with my MMA formulation. I have been using a formulation that I found in a published paper. My current embedding formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide. The samples are embedded after three days of infiltration, one change per day, with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have noticed that there is a pressure build up in the vials. I have had a few vials burst almost immediately once placed in the heated waterbath. I am filling the glass vials full with the embedding solution, capping them and then placing them in a water bath in a 37 degree oven. They are completely polymerized by the next morning. I am also getting bubbles in the plastic when polymerized. I have two questions: Is there any way to get rid of the bubbles in the plastic and of more concern what do you think is causing the pressure build up? I would really appreciate any help that you can provide. Thank you, Nikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Biopsy Processing Schedule
Oh Sorry, We have a Leica ASP300. Nikki -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Thursday, January 21, 2010 9:10 AM To: Wahlberg, Nikki; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biopsy Processing Schedule You will need to tell us what type of a tissue processor you will be using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, January 21, 2010 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Processing Schedule Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly appreciated. Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biopsy Processing Schedule
Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly appreciated. Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Steiner and Steiner
We already use the kit from Sigma but it is the 1% Uranyl Nitrate waste that is the issue. I will check with Newcommer to see if their kit will work. Thanks you! Nikki -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, September 24, 2009 1:44 PM To: Wahlberg, Nikki Subject: RE: [Histonet] Steiner and Steiner Nikki, What if you but the Sigma kit that has the 1% solution in it already? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, September 24, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Steiner and Steiner
Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo > - time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Thomas Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would > say that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or > close to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. > Truth is there isn't an abundance of HTLs out there. Unlike the > Medical Lab world, with the basic differences between MTs a
RE: [Histonet] MMA Dehydration
We are mainly processing vessels that can range in size of 10mm-200mm in length. The different types of vessels are either peripheral, cardiac or pulmonary trunks. Nikki From: Jack Ratliff [mailto:ratliffj...@hotmail.com] Sent: Tuesday, June 02, 2009 1:27 PM To: Wahlberg, Nikki Subject: RE: [Histonet] MMA Dehydration What is the tissue you are wanting to process??? Jack Ratliff > Date: Tue, 2 Jun 2009 13:19:49 -0500 > From: nikki.wahlb...@bsci.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] MMA Dehydration > > Hello Everyone, > > I am wondering if anyone would be willing to share their MMA dehydration > schedules? We are currently trying to improve our EXAKT MMA specimens. > We currently process with the following schedule: > 10% Formalin 4 hours > 70% Ethyl Alcohol 1 hour > 95% Ethyl Alcohol 2 hours > 95% Ethyl Alcohol 2 hours > 100% Ethyl Alcohol 3 hours > 100% Ethyl Alcohol 3 hours > > Thank you, > Nikki > > Nikki M Wahlberg > Histotechnologist II > Histo-Pathology Services > Boston Scientific > 763-694-5739 > wahlb...@bsci.com > > CONFIDENTIALITY NOTICE: This e-mail transmission may contain > confidential or legally privileged information that is intended only for > the individual or entity named in the e-mail address. Any unauthorized > distribution or copying of this transmittal or its attachments, if any, > is prohibited. If you have received this e-mail transmission in error, > please reply to the sender, so that Boston Scientific Corporation can > arrange for proper delivery, then please delete the message from your > inbox. > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MMA Dehydration
Hello Everyone, I am wondering if anyone would be willing to share their MMA dehydration schedules? We are currently trying to improve our EXAKT MMA specimens. We currently process with the following schedule: 10% Formalin 4 hours 70% Ethyl Alcohol 1 hour 95% Ethyl Alcohol 2 hours 95% Ethyl Alcohol 2 hours 100% Ethyl Alcohol3 hours 100% Ethyl Alcohol3 hours Thank you, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Turnbull's Blue
The study directors would like to use both the Prussian Blue and Turnbull's Blue to demonstrate two different types of iron in the tissue, ferric and ferrous. We are having trouble finding a control that contains both types of iron, thus we can not prove to the pathologist that are stain is indeed working. We in fact are not sure if the stain is working. Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com -Original Message- From: Lee & Peggy Wenk [mailto:lpw...@sbcglobal.net] Sent: Thursday, May 07, 2009 9:00 PM To: Wahlberg, Nikki; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Turnbull's Blue Turnball blue (i.e., Schmorl stain) demonstrates argentaffinic components, such as melanin or argentaffin cells in the GI tract. Prussian blue (i.e., the iron stain) demonstrates iron (hemosiderin). What are you trying to demonstrate? Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, May 07, 2009 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turnbull's Blue Is anyone performing the Turnbull's Blue Iron stain? If so, what type of control do you use. We are having a difficult time finding a good control in order to get the stain approved in our lab. Also, if anyone has some tips for the procedure they would be greatly appreciated. Thank you Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Turnbull's Blue
Is anyone performing the Turnbull's Blue Iron stain? If so, what type of control do you use. We are having a difficult time finding a good control in order to get the stain approved in our lab. Also, if anyone has some tips for the procedure they would be greatly appreciated. Thank you Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlb...@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
