[Histonet] Processing Canine Nasal Passages
Good Afternoon, I am looking for information on collection (ie. Upper jaw to eye socket?), decalcification(approximate timing, tips?), trimming (any tips/suggestions) and processing (without mega cassettes if possible) canine FFPE tissues. If someone can please give me some suggestions or ideas I would appreciate it. Or direction to an appropriate article? Thanks in advance. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Density in Rodent Femurs/Archimedes Principle
Hi All, I am not having much luck finding exact information to perform bone density on rat femurs/tibias using Archimedes principle. Can anyone provide an exact procedure, or point me in the right direction? Including exact type of equipment used, etc. I have found many references with outlined instructions, but not exact enough for what I need. Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alizarin Red for rodent feti
Hi All, Going in a different direction in this area, are there any reference labs out there that can process/examine these specimens? Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alizarin Red for rodent Feti
Good Day All, Can someone pass along methodology for processing rodent Feti for Alizarin Red stain and exam? I would appreciate any input/ direction given as this is a foreign topic to me. Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin sections or formalin fixed tissue for EM - looking for reference lab
Hi All, Can anyone recommend a reference lab that can use paraffin sections or formalin fixed tissue for EM? Thanks in advance for suggestions. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Slide Printers
We have had our slide printer for going on 6 years and have had minimal problems. It does require good maintenance habits though, as do all slide printers. We also purchased the PM/service contract on this and the cassette printer when the warranty ran out and I think they are worth the cost since service calls always seem to cost a lot even if it is something minor. Regards, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cresyl Violet as a counterstain
Hi All, Looking for suggestions or protocol for staining 20 micron rat brain sections with a Prussian Blue w/cresyl violet counterstain and a modified Wright Giemsa w/ Cresyl Violet counterstain. Any suggestions or tips to do this would be greatly appreciated. Both for dealing with these very thick sections and then also for the staining. Thanks in advance. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FDA Reagent Label Requirements for Richard allan Flex alcohols
Hi All, I currently got inspected by my QA dept. and they complained that our Flex alcohols did not have storage requirements on their labels, is anyone using the new Flex alcohols in the larger bottles and if so, do they have the storage requirements on the labels. If not, are there any GLP labs out there that add a label to their alcohols when they are received stating storage requirements? Looking for ideas to have a simple fix for this. Thanks, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GGT IHC in NHP
Hi All, Does anyone out there have experience staining for GGT in NHP tissue, specifically Rhesus monkey? If so, could you please forward any information you may have. I was told by a business manager that this is "just a standard special stain" , like an iron, PAS or Trichrome, and should be able to accommodate within our standard histology offerings. Thanks in advance for all help. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Muscle injection site in rats
Hi All, Is there a way to isolate the point of injection for histology on an intramuscular injection into a rat thigh? Any suggestions or protocols would be appreciated. Thanks, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cyclin D3
Hello All, Is anyone out there currently doing Cyclin D3? We are having trouble getting any staining at all. We are doing manual heat retrieval and staining. Any suggestions or protocols would be appreciated. Thanks in advance, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab to do Possible EM as well as preferred fixative
Hi All, We are going to be collecting rat ileum for possible EM after completion of our study. I am looking for a lab to possibly process these specimens as well as what most would consider the preferred choice of fixative and storage while we are holding the specimens until the decision is made to process them. Thanks in advance for replies. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Human tumors from mouse xenograft IHC HELP!
Can anybody out there provide protocols and/or vendors for the following antibodies for human tumors from mouse xenograft: ORC1L, MCM4, MCM6, MCM7, Cyclin D3, and FEN1? I am trying to show my boss that this is not as simple as he seems to think it is for a lab that is not basically set up to do even basic IHC. Thanks in advance... Carol Carol Wilson, HT(ASCP) Lead Technician/Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LFB on GMA sections
Hi all, Can anyone tell me if LFB stain works well for staining nerves in GMA sections? My pathologist is looking at sections of these stained elsewhere and she does not feel the myelin is staining as it should. Any insight on this particular combination? Thanks in advance. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Medical Equipment Source
I have dealt with Luigi and Wendy Mascio for several years now, and have always been pleased with my purchases as well as their customer service. I would not hesitate in recommending Medical Equipment Source. Carol Wilson, HT(ASCP) Lead Technician Histology - Associate Scientist III Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
