Re: [Histonet] gelatin
Thank you for your e-mail. Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan wrote: > You need to explain "treated tissue". > > Gelatin is collagen that has been boiled until the protein has lost all > its fibrous nature and changed into a water-soluble protein. Gelatin is > made permanently insoluble by adequate formaldehyde fixation. It is stained > by anionic dyes (such as eosin in the H&E method), but it does not show as > fibres when you look at the section or smear through a microscope. > > If this doesn't answer your question, please explain your problem and > involve your boss in future email exchanges. > > *John Kiernan* > London, Canada > = = = > > On 23/02/15, *Yak-Nam Wang * wrote: > > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gelatin
Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraformaldehyde vapour
Hello, In a protocol to stain mucin in oesophageal tissue, it calls for "post-fix in paraformaldehyde vapour at 37 deg C". The protocol is a modified Alcian blue/PAS staining technique. Can someone share insight into how to fix in paraformaldehyde vapour? That is, how much paraformaldehye powder is needed per container area/# of slides. Do I just place powder at the bottom of a dish with the slides and place in at 37 deg C? Thank you for your help Yak-Nam Wang University of Washington APL Seattle, WA 98105 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
Hi Louise, If your lab is like other labs and are a bit strapped for money but need basic point counting, you may also be able do this using ImageJ. ImageJ is a free piece of software developed at and provided by NIH. It has grid and point overlays and counting tools. The learning curve isn't very steep, and it's free! http://rsbweb.nih.gov/ij/ There is also a pc version. Good luck Yak-Nam Wang On 9/28/10 1:47 PM, "Wagner, Richard [BUL/LAK]" wrote: > Louise: > > Versatile image analysis software like OmniMet can solve this problem. > Powerful digital image acquisition, object and field measurement > options, and counting tools are standard features of the software. > http://www.buehler.com/productinfo/biomedical/ia.htm > > Regards, > Rick Wagner > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack > Ratliff > Sent: Tuesday, September 28, 2010 8:36 AM > To: louise renton > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] histomorphometry > > At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva > demonstrated this very technique in his workshop. My best advice would > be to contact him directly (avillanuev...@cox.net) and see what he can > do for you. Another option would be to contact an image analysis vendor > like BIOQUANT (nathan...@bioquant.com). Hope this information helps! > > Regards, > > Jack > > On Sep 28, 2010, at 3:25 AM, louise renton > wrote: > >> Hi all, >> >> I desperately need some advice from an experienced > histomorphometristI >> am trying to translate the old cumbersome visual measurement of using > an >> eypiece grid and doing point counting to an easier computer system - > but I >> am not sure how to do it - any help out there? >> -- >> Louise Renton >> Bone Research Unit >> University of the Witwatersrand >> Johannesburg >> South Africa >> +27 11 717 2298 (tel & fax) >> 073 5574456 (emergencies only) >> "There are nights when the wolves are silent and only the moon howls". >> George Carlin >> No trees were killed in the sending of this message. >> However, many electrons were terribly inconvenienced. >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formalin substitutes - tissue structure
Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. >From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] fume hood regulations
Hi, At the University of Washington we have been told to lower the sash all the way when the hood is not in use to save energy and money. I believe there have been several Universities that have done some cost analysis of this and have show large savings in money (one study stated $1500 per year per fume hood in savings and a reduction in 10,600 lbs of CO2 emissions). If you want more information just do a search for "shut the sash". So, if it is not feasible to shut off the hood you can save energy and money just by closing the sash. Have a great weekend. Yak-Nam On 5/21/09 10:10 AM, "Montina Van Meter" wrote: > Fellow Histonetters, >Does anyone have knowledge of guidelines or regulations on whether a > fume hood must remain "on" 24/7? In a cost cutting measure a facility > is considering turning off the fume hoods from 7pm to 5am. I have > expressed my concern with the idea because of the huge health and safety > issue for those who work after hours. This facility could be setting > themselves up for a huge liability law suit. I would appreciate > written guidelines to strengthen my opposition to this "cost cutting " > measure. > > Thank you, > > Tina > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Thanks Rene, I have heard of the sliding microtomes and a colleague thought that as long as I could fix and decalcify, it might be possible with one of these. I fear I may have to pre-cut the foot in to manageable portions before processing. Yes, if I find out how to do it I will definitely let you know! Yak-Nam On Fri, Apr 10, 2009 at 1:34 PM, Rene J Buesa wrote: > As far as I know such a large thin section that also involves decalcifying > hard bones has never been published. In the past E. Leitz (now Leica) > manufactured sliding microtomes for large specimens but were used mostly to > prepare thin brain sections embedded in celloidin but not for what you would > like to do. > If you are able to do it, for sure we all would like to know. > René J. > > --- On *Fri, 4/10/09, Yak-Nam Wang * wrote: > > From: Yak-Nam Wang > Subject: Re: [Histonet] Whole human feet > To: "Woodward, Denise" > Cc: "histonet@lists.utsouthwestern.edu" > > Date: Friday, April 10, 2009, 4:26 PM > > > Denise, > > Thanks, someone else pointed out these images and they are indeed in the > correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I > think these are 1 mm sections from a frozen body, unstained. We were > actually hoping that we could process feet (procured from a company), > section and stain the sections with the basic H&E, modified Hart's, > Picro > sirius red and such like. > > Any help would be greatly appreciated. > Thanks > Yak-Nam > > On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise > wrote: > > > I believe there is a computerized image file of an entire male and a > > female human body somewhere on the WWW. Maybe NIH?? These were whole > mount > > histologic sections. Don't know if the plane is correct for your needs > with > > regard to the sections of feet. News about it came out about 4-5 years > ago. > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > > Sent: Wednesday, April 08, 2009 12:07 AM > > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Whole human feet > > > > No offense, dude, but GROSS. > > > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > > > > > > > From: Yak-Nam Wang > > To: histonet@lists.utsouthwestern.edu > > Sent: Monday, April 6, 2009 8:04:54 PM > > Subject: [Histonet] Whole human feet > > > > Hello all, > > > > If possible, we would like to obtain histological sections of adult human > > feet (plane of the anterior-posterior surface). Does anyone know of > > labs/groups that have done this or something similar? > > > > Thank you for your help > > Yak-Nam Wang > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing > listhisto...@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic H&E, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise wrote: > I believe there is a computerized image file of an entire male and a > female human body somewhere on the WWW. Maybe NIH?? These were whole mount > histologic sections. Don't know if the plane is correct for your needs with > regard to the sections of feet. News about it came out about 4-5 years ago. > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > Sent: Wednesday, April 08, 2009 12:07 AM > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Whole human feet > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > From: Yak-Nam Wang > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Correction, we would like the cut to be dorsal to plantar surface (e.g. through the metatarsals), not anterior to posterior (that would be a rather large section!) Cheers! Yak-Nam On 4/6/09 5:04 PM, "Yak-Nam Wang" wrote: > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole human feet
Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
