[Histonet] ANP.21460

2013-09-12 Thread anita dudley
would someone please share how they document the positive tissue controls, we 

check them all before going out of the lab and marked we have checked. what

else are people doing?  thanks so much for your input

 

Anita Dudley

Providence Hospital

Mobile Alabama
  
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[Histonet] Cryostat decontamination

2013-08-16 Thread anita dudley
Just curious how often everyone is taking their cryostats down,  I know cap 
says every week if used daily.  Thanks ahead for the info.  Everyone have a 
great weekend

 

Anita Dudley

Providence Hospital

Mobile Alabama
  
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[Histonet] Hardware from surgery

2013-08-02 Thread anita dudley
What do others do with hardware from surgery?  Do you hold on to it for a 
longer period of time because of a possible legal case?   Thanks so much, 
everyone have a great weekend!!

 

Anita Dudley

Providence Hospital

Mobile, Al. 36695
  
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[Histonet] CAP 23045

2013-07-24 Thread anita dudley
Can someone please tell me are you adding this to your instrument procedure or 
should there be a seperate procedure for each instrument?  Thanks so much,  
Anita Dudley
  
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[Histonet] cap anp.23120

2013-05-07 Thread anita dudley
Wondering what people are doing about the new cap anp.23120?  validating tissue 
processing schedules.  Thanks a lot
 
Anita Dudley
Providence Hospital
Mobile, Alabama   
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RE: [Histonet] CJD processing

2012-08-23 Thread anita dudley

Jennifer,  We have in our procedure to send any sucpected specimen to the 
National Prion Disease Pathology Surveillance 
center  at Case Western University in Cleveland Ohio.  the phone number is 
216-368-0587.  Hope this may help.
 
Anita Dudley
 

> To: histonet@lists.utsouthwestern.edu
> From: jmacdon...@mtsac.edu
> Date: Wed, 22 Aug 2012 22:31:21 -0700
> Subject: [Histonet] CJD processing
> 
> Does anyone know of a lab in Southern California that is equipped to 
> handle CJD (suspected) cases?
> Thank you,
> Jennifer
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[Histonet] negative controls

2012-08-01 Thread anita dudley

just wondering if we can start leaving the negative controls off of our immuno 
stains now as long
as our procedure says we can stain without them?   we use ventanas multimer 
detection systems.
 
thanks so much,
anita dudley
providence hospital
mobile,  alabama
  
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[Histonet] ventana uv alkaline phosphatase red

2012-03-13 Thread anita dudley

for those of you that use the ultraview alk phos red on their machines,  do you 
run the slides through
alcohols?   we are having a lot of trouble with the staining,  sometimes it is 
good others no staining
at all on our mart 1s  or pin4 prostate bxs.
I was told that we should not put them in alc.  air dry after water or oven 
dry?  just wondering is
that how others are handleing their slides stained with the red kit?  we have 
been running them down
through the alc. with the dab ones.
 
thanks so much
anita dudley
providence hosp
mobile alabama
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[Histonet] new billing

2012-02-14 Thread anita dudley

can someone explain the new billing for the antibodies on blocks?  not sure
what they mean.  can you just bill one antibody per block even tho
you may do three or four?   thanks so much
 
anita dudley
providence hosp
mobile alabama
  
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[Histonet] cryostats decontamination

2012-02-08 Thread anita dudley

Is everyone taking their crostats down weekly??  just seems overkill to me
 
thanks,
anita dudley  
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RE: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus

2011-10-31 Thread anita dudley

allison,  we do all of our alcian blue on the machine.  we love it!!
anita dudley
providence hosp
mobile, ala
 

> From: allison_sc...@hchd.tmc.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 21 Oct 2011 14:00:44 +
> Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus
> 
> Hello to all in histo land. For those of you that have the ventana special 
> stains machine, can the alcian blue 2.5 be done on the machine? I am not sure 
> if they have kits for this stain. Also is anyone using the cryoembedding 
> apparatus from pathology innovations? Thanks in advance.
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital.
> 
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[Histonet] er-pr validation

2011-10-19 Thread anita dudley

Is there anyone our there that would like to exchange slides or blocks to do 
er-pr validation?
we would be interested.
thanks
anita dudley
2600 tahoe drive
mobile, alabama
251-633-1422  
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[Histonet] cryostats

2011-10-18 Thread anita dudley

what are people using to disinfect the cryostat, and is everyone taking their 
machine
down weekly as the CAP recomends?
thanks for the info
anita dudley
providence hosp
mobile alabama
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[Histonet] new antibody lot

2011-09-22 Thread anita dudley

how are others handling new lot numbers of antibodies when you receive them in 
the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
  
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[Histonet] er pr validation

2011-07-19 Thread anita dudley

I know this has been talked about before here but how many slides are 
people doing for er pr validation,  and are you doing it with another hosp?
thanks so much for your help.
 
anita dudley
providence hosp
mobile al 
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[Histonet] cassette and slide labelling

2011-05-27 Thread anita dudley

I have another question.  what cassette and slide labeling systems are people 
using out there.  we have 50 to 60 case load 
a day.  thanks,  just wondered what people use.   thanks again,  anita, 
providence hosp.  mobile, alabama 
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[Histonet] hollande fixative

2011-05-26 Thread anita dudley

this has come up before but I am going to ask for those of you that use 
hollande for gastric bxs, do
you wash before you put them on the machine?  and what do you wash them with?
 
thanks so much!!!
 
anita dudley 
providence hosp.
mobile, ala   
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RE: [Histonet] ventana validation

2011-05-12 Thread anita dudley

thanks linda I think that sounds good,  I am going to do that. 
 
anita
 
> Subject: RE: [Histonet] ventana validation
> Date: Thu, 12 May 2011 13:44:52 -0500
> From: lseb...@uwhealth.org
> To: azdud...@hotmail.com; histonet@lists.utsouthwestern.edu
> 
> We run a control with the new kit and compare it with a control that has
> been run with the current kit (kept in a detection kit QC file). We
> keep it as simple as possible with the least amount of cost we can
> manage. 
> 
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
> dudley
> Sent: Thursday, May 12, 2011 12:55 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] ventana validation
> 
> 
> would someone send me a copy of their validation that they do for the
> dab or red ultra view kits? I
> am not sure how to do this on the machine, do you do a control one one
> kit and the same on the new?
> just not sure what to do. thanks so much in advance
> 
> anita dudley
> providence hosp
> mobile alabama
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[Histonet] ventana validation

2011-05-12 Thread anita dudley

would someone send me a copy of their validation that they do for the dab or 
red ultra view kits?  I
am not sure how to do this on the machine,  do you do a control one one kit and 
the same on the new?
just not sure what to do.   thanks so much in advance
 
anita dudley
providence hosp
mobile alabama
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[Histonet] ANP 11630

2011-05-05 Thread anita dudley

just wondering if anyone out there would send their procedure for how they 
handle this. 
do you have a list with each type the tech can put on and if it is indirect or 
direct.  thanks 
so much.
and also is everyone taking their cryostats down every week and if not have you 
been
cited?  
thanks
anita dudley
providence hosp
mobile, al
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RE: [Histonet] Her/2

2011-05-05 Thread anita dudley

marcia,  was just getting ready to stain our her 2's ,  will let you know how 
they look.
 
anita dudley
 providence hosp
mobile,  al
> Date: Wed, 4 May 2011 12:54:29 -0400
> From: fu...@mercyhealth.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Her/2
> 
> CAP validation Her/2 slides not staining as clear as before. Is anyone else 
> having any issues ? We use the Ventana system and our daily IHC slides are 
> staining
> and controls are working well. Is there a coating on these slides from CAP ? 
> Thanks for any help ?
> Marcia Funk HT,QIHC
> 
> 
> 
> Marcia Funk 
> Histology Laboratory
> Mercy Medical Center North Iowa
> Mason City, IA, 50401
> 641-428-7907
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[Histonet] cap new ANP.22760

2011-04-29 Thread anita dudley

hi everyone,  just wondering how others were handling this checklist statement? 
 thanks so much for your input.  seems 
like a lot of extra work when running control with each show if each is working.
 
anita dudley
providence hosp
mobile alabama
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[Histonet] billing

2011-01-21 Thread anita dudley

for those of you that are doing her2 immunos,  are you charging more for these 
than for other antibodies?  I pay way more for these, and it seems we should be 
able to charge more?  thanks,  everyone have a great weekend!!
 
anita dudley
providence hospital
mobile, alabama 
  
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[Histonet] hollande solution

2010-08-30 Thread anita dudley

for those of you that use hollande sol for gI bx's do you wash the bxs before 
putting them in formalin? we started doing that thinking it would help. our 
pathologist are complaining the stain is not crisp on the gI's.  we change and 
rotate our stainer often.  do you run them on a short run?  I am thinking that 
may help.  is anyone using zinc fixative for them?
 
any input would be helpful,  thanks so much.
anita dudley
providence hosp
mobile alabama
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[Histonet] new er pr cap guidelines

2010-04-20 Thread anita dudley

has anyone seen the new er pr guidelines for er and pr?  my pathologist just 
came through all in a tizzy about it.  just wondering if anyone else has seen 
it.  the fixation times were also suppose to change,  to 72 hrs fixation for 
breast.

 

thanks for anybodies input,  anita, providence hosp. mobile alabama
  
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[Histonet] her2 validation

2010-03-30 Thread anita dudley

sorry!!!  I didn't put a subject in the previous post.  wondering about how 
many slides people are using for her2 validation.  thanks

 

anita dudley 

providence hospital

mobile alabama
  
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[Histonet] (no subject)

2010-03-30 Thread anita dudley

just wondering, how many slides are people doing for her2 validation.  cap says 
25 to 100, I have got 25 but my pathologist thinks we should do more?  thanks, 
anita  
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[Histonet] number of slides

2010-03-23 Thread anita dudley

just wondering what others were doing with colon bxs. embs, eccs.  do you use 
one slide or cut 2 to 3 slides per block?  lungs and livers too.  thanks, we 
are thinking of going to one slide.  

 

anita dudley

providence hosp.

mobile alabama
  
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[Histonet] negative controls

2010-03-03 Thread anita dudley

we have a ventana benchmark and use rabbit and mouse negative controls, is 
there  anyone out there that uses a universal control and is that ok with your 
cap inspection?  thanks so much

 

antia dudley

providence hosp

mobile alabama
  
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[Histonet] cryostat decontamination

2010-02-22 Thread anita dudley

thank you all for posting about the cryostat decontamination,  I have not 
revised my procedure and we are due for an inspection .  I think this is just 
over kill, our pathologist says we have to do it if cap says. (they also made 
me throw away all of the dry drys that they said were outdated?  now I think 
they have changed that, I thought that was crazy also.) the way we do it here 
is if the path thinks it should be down they will tell us.  they try to just do 
smears if it is a know infectious case.

I have another question for those who use ventana's benchmark, or I guess 
anybody that is doing imunos. are you using a universal negative control?  or 
positive and negative with most every case?  thanks for your input.

everyone have a good day.

 

anita dudley

providence hosp

mobile alabama
  
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[Histonet] cleaning cryostat

2010-02-04 Thread anita dudley

we are coming up for our cap inspection and I need to write the procedure over 
for cleaning the cryostat once a week??  I think it is overkill to take it 
down every week but that is what it says.  what is everyone else doing and what 
do you use to disinfect it?  we now every week wipe it with 70% alc. but we do 
not take it all down.  I know we have had things on about this before but just 
wanted to update.  thanks so much

 

anita dudley 

providence hosp

mobile alabama
  
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[Histonet] cap question amp.12087

2009-12-30 Thread anita dudley

just wondering if everyone is defrosting their cryostats every week?  seems 
like overkill, we clean ours out daily and every week clean with 100 alc.  what 
are others doing?  this is a revised question.  thanks a lot  

anita dudley

providence hospital

mobile
  
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[Histonet] her 2 validation

2009-12-02 Thread anita dudley

good morning!!  just wanted to make sure about the number of slides that are 
required for validation for her 2.  is it 25?  thanks so much, 

 

anita dudley

providence hospital

mobile alabama
  
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RE: [Histonet] Sakura Products

2009-08-26 Thread anita dudley

emily,  we have a sakura processor, embedding center, an accu-cut SRM microtome 
and a coverslipper.  they are all great. you can't go wrong with sakura.  anita
 
> Date: Tue, 25 Aug 2009 08:54:22 -0400
> From: em...@pathlabsolutions.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sakura Products
> 
> Hi All:
> 
> I'm looking to purchase equipment for a new lab and would like the opinion
> of anyone who has worked with various Sakura products before... good or bad.
> I would be looking to buy the following products:
> 
> Tissue-Tek TEC 5 Embedding Station
> Accu-Cut SRM 200 Microtome
> Cyto-Tek Cytocentrifuge
> 
> Also if anyone has experience with prostate and GI bx processed in the
> Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that
> too.
> 
> Thanks!
> 
> -- 
> Emily Butte, M.S., HTL (ASCP)
> Path Lab Solutions Inc.
> (701) 371-7515
> em...@pathlabsolutions.com
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[Histonet] richard allan dehydrant

2009-05-20 Thread anita dudley

just wondering if anyone had richard allans 95 and 100 dehydrant on the 
processor and if they had any problems.  I have switched from flex, our 
cytology dept uses the dehydrant and we share the same room.  anyway our little 
bxs looked a little fried today,  the oven was up a little also so it might 
have been that.  would appreciate any input on the dehydrant.  thanks

 

anita dudley

providence hosp

mobile, al 36695

 

 

 

 

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[Histonet] ventana

2009-04-27 Thread anita dudley

just wondering for those who have the benchmark, do you print out the reports 
for every run and have the dr.s sign?  we were told the every line on the form 
needs to be signed.  it is a lot of extra writing we feel and are trying to 
come up with a qa sheet for immuno, special stains and h&e all on one.  does 
anyone have any suggestions?  thanks

 

anita

providence hosp

mobile alabama

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[Histonet] tissues and static

2009-03-27 Thread anita dudley

has anyone had any trouble when embedding little specimens they float to the 
sides of the molds like they are charged.  it happens sometimes with our little 
core bxs.  thanks,  everyone have a good weekend!  

 

anita dudley

providence hosp

mobile alabama

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[Histonet] wondering if anyone else has had this problem? wh...

2009-03-23 Thread anita dudley

wondering if anyone else has had this problem?  when embedding small core bxs 
they are like they are charged and go off of the forceps to the edge of the 
embedding mold.  its like they are running away from the forceps.  what would 
be causing this?  thanks so much.

anita dudley
providence hosp
mobile, alabama

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[Histonet] trichromes on ventana nexeES

2008-11-07 Thread anita dudley

we are doing trichromes for our microbiology dept on their stool samples.  is 
anyone doing this?  we are having trouble with the controls that they purchase 
staining on the slide.  just wondering if anyone else is doing this and what 
they have done to keep the spec on the slide.  the pt slides seem to be fine.
thanks a lot,   everyone have a good weekend!!!   
 
anita dudley
providence hosp
mobile alabama
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[Histonet] IHC QA

2008-10-29 Thread anita dudley

anyone out there that has a ventana benchmark XT,  how do you handle the 
reports for the pathologist to sign off on when you have more than one path?  
do you just print one for each dr. or do then pass it around and sigh off on 
their case?
we had a joint com. person tell us that we could not have an empty space?  we 
now send one to each dr., they sigh their cases and we file all the sheets.  
there are enpty spaces where one signs and the other has signed in another 
place. what are others doing?  
thanks for the help.  we could have them pass the report around but I know that 
won't work very well.
thanks again,  everyone have a good halloween!!!!  
anita dudley
providence hosp
mobile alabama
 
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RE: [Histonet] out dated reagents

2008-10-09 Thread anita dudley

to all!!!  thanks so much for your responses to my question about outdated 
reagents,  that helped me, cause I think it is such a waste for throw 
everything out, I am going to show them to my director. amy,  our cytolotist go 
to the FNA's and they label everything at the bedside. the path only goes when 
the cytotech has all the slides ready for them to look at.  that is all written 
in the cyto dept procedure for FNA.  hope this helps, anything else just ask.   
thanks again all,  have a good weekend,  anita> Subject: RE: [Histonet] out 
dated reagents> Date: Tue, 7 Oct 2008 15:38:48 -0400> From: [EMAIL PROTECTED]> 
To: [EMAIL PROTECTED]> > Anita,> Our inspection is any day now and I have cone 
across a checklist> question that has stumbled me. Do your pathologist do FNA 
procedures?> And if so do you have anything that you could share with me 
regarding> this question from the CAP checklist below: If the pathologist> 
performs FNA procedures, is there a documented procedure to prevent> errors in 
the identification of the patient, the site and the procedure?> > > Any info or 
help would be greatly appreciated. > > Thanks amy> > > > -Original 
Message-> From: [EMAIL PROTECTED]> [mailto:[EMAIL PROTECTED] On Behalf Of 
anita> dudley> Sent: Monday, October 06, 2008 12:19 PM> To: 
Histonet@lists.utsouthwestern.edu> Subject: [Histonet] out dated reagents> > 
hello all, we were inspected the beginning of june by cap and one of> our 
deficiency was anp.21382. it had to do with outdated reagents. my> director 
want all of my dry chemicals gone I have gone through> them and just have 
what I need for the special stains that we do in> house. does anyone know do 
they put expiration dates on dry chems. we> have things like light green, 
biebrich scarlet, periodic acid. I really> hate to get rid of all of this but 
he thinks I should.> > what are others doing about this? I can see after you 
have made up> things to not keep it long but dry I know many yrs ago the 
biological> stain commission thought it would be meaningless and arbitrary to 
put> expiration dates on stains. it would me more important to maintain a> 
record of the use of the powdered stain. which is what we do when we do> the 
stain with our controls.> > anyway just wondering if there were any thoughts 
out there about this. > > anita dudley> providence hosp> mobile alabama> 
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[Histonet] (no subject)

2008-10-09 Thread anita dudley

boy sorry guys,  that last message sure doesn't look right. don't know what I 
did.  amy if you can't make it out let me know.   anita
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[Histonet] out dated reagents

2008-10-06 Thread anita dudley
hello all,  we were inspected the beginning of june by cap and one of our 
deficiency was anp.21382.  it had to do with outdated reagents.  my director 
want all of my dry chemicals gone   I have gone through them and just have 
what I need for the special stains that we do in house.  does anyone know do 
they put expiration dates on dry chems.  we have things like light green, 
biebrich scarlet, periodic acid.  I really hate to get rid of all of this but 
he thinks I should.
 
what are others doing about this? I can see after you have made up things to 
not keep it long but dry I know many yrs ago the biological stain commission 
thought it would be meaningless and arbitrary to put expiration dates on 
stains.  it would me more important to maintain a record of the use of the 
powdered stain.  which is what we do when we do the stain with our controls.
 
anyway just wondering if there were any thoughts out there about this.  
 
anita dudley
providence hosp
mobile alabama
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[Histonet] job opening

2008-09-17 Thread anita dudley
sorry I failed to put anything in the subject line on the previous note.  anita 
  job is in fairhope ala. mohs lab in need of a histologist.  251-633-1422
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[Histonet] (no subject)

2008-09-16 Thread anita dudley
job in fairhope alabama, beautiful area along the bay. dematologist office 
looking for someone to do the mohs surgery.  contact anita, providence hosp.  
mobile alabama,  251-633-1422. 
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