Re: [Histonet] Question for Labs performing Electron Microscopy
It's the same for me here. But then again, we're pure research. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 > We used to do TEM at our lab. The work volume was not high and one of > histotechnologists was trained to do ALL tasks (fixation â printing the > photos of the areas selected by the pathologist). > When not doing TEM tasks, he took care of any of any of all HTL tasks for > which he was also trained. > René J. > > > On Wed, 3/19/14, Gauch, Vicki wrote: > > Subject: [Histonet] Question for Labs performing Electron Microscopy > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 19, 2014, 10:25 AM > > Hi everyone, > I have been asked to post the following question regarding > EM... > If you are providing EM services in your laboratory, who is > performing the EM, what is their job title and what are > their job duties ( do they process, embed, cut,stain,etc. or > just certain portions ). > Any information would be greatly appreciated... Please > feel free to e-mail me directly or through the Histonet ... > > Thanks, > Vicki Gauch > AMCH > Albany, NY > > > - > CONFIDENTIALITY NOTICE: This email and any attachments may > contain confidential information that is protected by law > and is for the sole use of the individuals or entities to > which it is addressed. If you are not the intended > recipient, please notify the sender by replying to this > email and destroying all copies of the communication and > attachments. Further use, disclosure, copying, distribution > of, or reliance upon the contents of this email and > attachments is strictly prohibited. To contact Albany > Medical Center, or for a copy of our privacy practices, > please visit us on the Internet at www.amc.edu. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Equipment recommendations, please
Which microtomes and embedding centers are you using out there? I am looking at the Leica RM2265 (since we're a research lab) and the Thermo Scientific Finesse (We have one and I like it!), but I am also open to suggestions. Thank you so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing thin brain slices in. Histogel
We are trying to process thin slices of mouse cerebellum embedded in Histogel into paraffin, and we have encountered two problems: 1) the Histogel button with the slices inside it has been curling up in the processor ( before anyone asks, we're using our own cassettes, not the histoscreen ones that come in that starter kit), and pressing it flat with weights while embedding puts stress on the tissuehas anyone else seen this? 2) The Histogel, once embedded in paraffin, has occasionally chipped out of the block on sectioning. This, to me, implies an infiltration problem, but this stuff is supposed to be compatible with paraffin processing. Why would it not infiltrate? We ran the buttons on the biopsy program that came with our VIP 5. If you want details, I'll send them once the processor is done. The Histogel is still within its expiration date, and the curly batches were done with a fresh tube of Histogel from the fridge. Any and all ideas/suggestions/solutions are most welcome. Happy Friday, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing Drosophila brains into paraffin
I was given a protocol for processing Drosophila brains into paraffin manually, and it involves methyl benzoate. It is as follows: Fixation: 3.5-4 hours in Carnoy's fixative, in hood (room temperature) Processing: 2x30 min 99% ethanol (Would 95% be okay?) 60 min in absolute ethanol Overnight in methyl benzoat (that's how it's written in the protocol), can be up to 3 days (*Here is my question-I looked on Sigma's website for this, and found a bunch of similar chemicals, but no straight methyl benzoat. What do I order?) 1 hour methylbenzoat-paraffin 1:1 mix at 60 degrees C 6x20 min 60 degrees C paraffin (we use Paraplast X-tra) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing thin slices of mouse cerebellum
To all, We have these very thin slices of mouse cerebellum that need to be processed into paraffin. The problem is that they need to be kept flat. We have tried sponges, but some of the slices are so small that they shrink (processed with our biopsy program, of course; if you want details, let me know) and get lost in the holes of the sponges. We have biopsy cassettes, but the slices will still tumble and not stay flat. We have processed other things in lens paper, but I had to scrape the tissue with the paraffin into the mold. I have not tried Histogel, though-maybe embed the slices flat in that first and then process it? Is there anything else? Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin)
I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > We have both and love them both. IF you are using tape coverslips then > perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica > and Sakura and find fewer problems with the Leica. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact > HistoCare > Sent: Wednesday, March 20, 2013 6:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a > simple interface while the Sakura has a LCD screen with detailed > information about what stage the staining process a rack is along with > multiple menus. The difference between the performance changes > drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The > Leica's coverslipper is its Achilles heel and requires a LOT more > attention and alerts frequently, very frequently. It takes a separate rack > for staining the slides at the beginning of the process and eventually > transfers them to a different rack one the cover slip is complete. This > one uses glass and frequently drops glass, creates bubbles, drops and > breaks slides. You will have to frequently purge the system and clean the > cover medium needle dropper. Once done, it only holds. Two racks of 30 > slides and will alert until you remove it. You can't leave this one alone > for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced > not even remotely as soon as the glass in the Leica. Finished slides > remain in a carousel at the top and can hold about 10 racks of 20 before > it alerts. For high volume, the Sakura pair wins hands down. You won't > lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for either > a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any > recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to meet > our workload. The incorporated oven seems excellent on both stainers. Any > pros/cons would be greatly appreciated! Also, if you are currently using > the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Has anybody ever used...
Leica's CoolClamp, or Thermo's Cool Cut? These sound like a possible good solution for the inevitable days when the aging AC plant here can't keep up with the summer heat. Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome upgrade, planning stages...
We are starting to look into upgrading to "higher-end", but used, microtomes. What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could these samples have been saved?
I don't know about your samples now, but for future reference, you might find this interesting: http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > Dear all, > > During an overnight tissue processing cycle, a malfunction occurred such > that the sample basket was suspended in mid-air for several hours at > probably the worst spot in which to do so---after the final absolute > ethanol of the dehydration series. I continued the process manually in the > morning, and carried it through blocking and attempted sectioning. > However, the samples (mouse skin and fat) had been converted to uncuttable > rocks. > > In hindsight, should I have attempted to rehydrate and reprocess these > samples in an attempt to glean even minimal information from them? Or is > there no way to unmummify a mummy? > > Regards, > > Kevin Johnson > University of Miami > Diabetes Research Institute > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking at cryostats
What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Number of blocks
Granted, I am in research, and my boss IS a pathologist and does know how to embed...if I got that reaction from a pathologist, I'd make him come in and embed, cut, etc. rank be damned. You need to know what is going on with the slides you're diagnosing. If a pathology resident/pathologist over at UMDNJ wanted to learn, I'd teach him/her, no questions asked. In fact, we have taught the Rutgers veterinarians here-invited them to sit in on our Toxicological Pathology class, both lecture and lab sections, slide reading, etc. and they made the time to do it. It's a necessity, the way I see it. Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > As I asked before, do your pathologists have any input into any of this? > > About embedding: I heard of a recently trained pathologist who, asked > about an embedding problem, replied, "What's embedding?" > > We spend thousands of dollars on a bronchoscopy or an EGD to get a > tiny bit of tissue that contains a life-changing diagnosis. The > specimen comes to the pathology lab and is grossed by a prosector who > isn't allowed an embedding sheet. The embedder has no idea how many > bits of tissue to look for. Then the microtomist is expected to cut 50 > blocks an hour. Then the pathologist has to make a diagnosis on a > venetian-blind section. > > Good Management I'm sure. Bad medicine. > > Bob Richmond > Samurai Pathologist > Maryville TN > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rene's papers
Rene & Richard, I hope you don't mind, but I had 5 minutes to do this. :o) How much formalin is enough to fix tissues? Buesa RJ, Peshkov MV. Ann Diagn Pathol. 2012 Jun;16(3):202-9. Epub 2012 Apr 5. PMID:22483550 [PubMed - indexed for MEDLINE] Related citations 2. - [Complete elimination of xylene in practice of a histology laboratory]. Buesa RJ, Peshkov MV. Arkh Patol. 2011 Jan-Feb;73(1):54-60. Russian. PMID:21506345 [PubMed - indexed for MEDLINE] Related citations 3. - Current status of cytology laboratories in anatomic pathology departments. Buesa RJ. Ann Diagn Pathol. 2010 Oct;14(5):347-54. PMID: 20850698 [PubMed - indexed for MEDLINE] Related citations 4. - Coverslipping without xylene. Buesa RJ. Biotech Histochem. 2010 Aug;85(4):269-70. No abstract available. Erratum in: Biotech Histochem. 2011 Jun;86(3):208. PMID: 20648958 [PubMed - indexed for MEDLINE] Related citations 5. -- Staffing benchmarks for histology laboratories. Buesa RJ. Ann Diagn Pathol. 2010 Jun;14(3):182-93. Epub 2010 Apr 2. PMID: 20471564 [PubMed - indexed for MEDLINE] Related citations 6. --- Productivity standards for histology laboratories. Buesa RJ. Ann Diagn Pathol. 2010 Apr;14(2):107-24. PMID: 20227016 [PubMed - indexed for MEDLINE] Related citations 7. --- Adapting lean to histology laboratories. Buesa RJ. Ann Diagn Pathol. 2009 Oct;13(5):322-33. Epub 2009 Aug 14. PMID: 19751909 [PubMed - indexed for MEDLINE] Related citations 8. Histology without xylene. Buesa RJ, Peshkov MV. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. PMID:19608083 [PubMed - indexed for MEDLINE] Related citations 9. - Histology aging workforce and what to do about it. Buesa RJ. Ann Diagn Pathol. 2009 Jun;13(3):176-84. Epub 2009 Mar 9. PMID: 19433297 [PubMed - indexed for MEDLINE] Related citations 10. -- Histology without formalin? Buesa RJ. Ann Diagn Pathol. 2008 Dec;12(6):387-96. Epub 2008 Sep 9. PMID: 18995201 [PubMed - indexed for MEDLINE] Related citations 11. -- Salaries in histology. Buesa RJ. Ann Diagn Pathol. 2008 Apr;12(2):122-7. Epub 2008 Jan 11. PMID: 18325473 [PubMed - indexed for MEDLINE] Related citations 12. Histology safety: now and then. Buesa RJ. Ann Diagn Pathol. 2007 Oct;11(5):334-9. PMID: 17870019 [PubMed - indexed for MEDLINE] Related citations 13. Microwave-assisted tissue processing: real impact on the histology workflow. Buesa RJ. Ann Diagn Pathol. 2007 Jun;11(3):206-11. Review. PMID: 17498595 [PubMed - indexed for MEDLINE] Related citations 14. Histology: a unique area of the medical laboratory. Buesa RJ. Ann Diagn Pathol. 2007 Apr;11(2):137-41. PMID: 17349575 [PubMed - indexed for MEDLINE] Enjoy! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
I second that! I have an older model of the Leica stainer, but it's still great. Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 FAX (732) 445-6905 > Leica Autostainer XL with CV5030 coverslipper and transfer station. > > This has been a real timesaver for us. It automatically moves the > slides from the stainer to the coverslipper. It has been relatively > trouble free. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe > Hardin > Sent: Wednesday, July 18, 2012 3:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] automated H+E and cover slipper > > Hi All, > I will be trying out new H+E autostainers and cover slippers soon. Does > anyone have a favorite, and why? Thanks for your responses. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: respirators and routine histology
Under normal circumstances, I don't wear a respirator for routine histology, as our airflow is very good. I attempted it when I was pregnant with my daughter...but it didn't last long as the mask pressed on a certain spot under my chin and made me want to throw up all the time, even after the morning sickness had passed. After that, I passed fume-intensive tasks that could not be done under the hood (like changing the processor)to coworkers and stayed out of the room while they were being done. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica CV5030 and Autostainer XL
To all, We got these at auction, and I am currently figuring out a) where to put them and b) how they go together, and of course there isn't a whole lot of information in the user manuals about setting these things up. I know, we should go to Leica for help, but my boss wants me to try it first. Would anybody in NJ happen to have these machines, and if so, may I come take a look at them? Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Routine H & E stain,
Amita, We are a neurotoxicology lab that also provides histology services for other researchers inside & outside Rutgers, and we use Gill's #3 hematoxylin for our routine H&Es on mouse and rat tissues. No fading that I have noticed in all the years that I have been here. We get ours from Thermo Fisher, or whatever they call themselves nowadays. :o) Good luck, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Hello Histonetters, > > I am from toxicopathology lab where we perform H&E on rat tissues and > store these slides for 10 long years. > > We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ > Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. > The staining result is good (Purplish pink look stained slide) on all > tissue, but our observation is after 5-6 months time this get faded and > become towards pinkish type though we can observe the slide. > We want to retain its fresh stained color. > > Please suggest me how to keep stable stain for longer period or do you > suggest to switch to any other type of haematoxylin - prepared or > commercially available? > > Looking forward for your feed back. > Amita > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
I use forceps, the thinner the tips, the better. I've had people in here who use brushes, too. I can't use my fingers, the ribbons always stick. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Same here. One tech keeps her index fingernail extra long for this > purpose. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila > Haas > Sent: Tuesday, March 08, 2011 8:26 AM > To: Bartlett, Jeanine (CDC/OID/NCEZID); > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] microtome safety > > > Every tech in my facility uses something different. Some use fingers, > some use > forceps (usually curved), one uses a teasing needle and one uses a paint > brush. > It's up to the individual's technique but we do try to discourage using > one's > fingers too close to the blade. > Hope this helps. >  > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. >  > > > > > > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tue, March 8, 2011 8:20:16 AM > Subject: [Histonet] microtome safety > > Morning all! > > I need some quick responses to this question: do you use your fingers or > an > instrument of some sort to pull your paraffin ribbons off the block when > sectioning? For those that do not use their fingers, what do you use? > If > forceps, are these the typical lab forceps or a special type? > > Thanks so much! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LKB Bromma 7800 knifemaker
Yes, I know this is ancient. But does anyone know of a service company that can repair this machine? Ours works, sort of, but it's very frustrating. Thanks and have a good weekend, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chatter or waviness of sections under the 'scope
To all, I am having a problem with either chatter or waviness of my sections when I look at them under the microscope. I've had chatter before, but in these sections, the chattered areas of tissue are literally standing up, giving the section a 3D look. The waviness is the same thing, except the tissue is in one piece-a 3D appearance under the microscope, no matter what tissue I have cut. (It kind of looks to me like the way bacon curls up in the pan when it's cooked-the fat in the bacon curls up and the meat mostly remains flat.) So far I have seen it in mouse kidney, liver and trachea. Have my Ultrastick slides lost their charge, is it static electricity, bad infiltration, or something else? If you need to see a picture,let me know & I'll go take one & post it. Thank you so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!!
This looks similar to a scam documented on Snopes.com, a website that clears up rumors, scams and urban legends: http://www.snopes.com/fraud/distress/family.asp Check out the second story in this category...very similar, no? Kathleen > Sorry Histonetters, but this needs to be cleared up! OK, Jerry > Santiago, are you alive and well in FL or the UK in trouble, or is > this another ridiculous scam I know how well connected you are, > so one of your colleagues should know... > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3...@yahoo.com > > On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: > >> Hi, >> My wife got scammed with something almost the same a month ago, >> less detailed message in hers though! They got into her gmail and >> deleted all her contacts and tried to delete all her most recent >> emails (they forgot to clear out her trash though, so she got most >> back). >> Don't fall for it! >> -Eric >> >> Research Tech 1 >> Program in Memrane Biology >> Mass Genreal Hospital >> >> On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: >> >>> I am rethinking this email since I read it over again. The scam >>> email I received asked for money ($1,800) to be sent to England >>> via Western Union. I am not sure about this since this supposed >>> Jerry is asking for just a response email. I would still be very >>> cautious! >>> >>> The Idaho news said the lady received a "yahoo request" (same as >>> mine) asking for email address, password, country of origin, >>> mothers maiden name, ect. Once the perpetrator had the >>> information, they immediately extracted all her information and >>> cleaned her bank accounts out. This was a business computer >>> account and it shut down her business! >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3...@yahoo.com >>> >>> On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: >>> Thanks, I deleted the message, now I am going to run a anti virus program. Valantou ** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 10/5. Please enquire at i...@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. * From: Akemi Allison [mailto:akemiat3...@yahoo.com] Sent: Tuesday, August 17, 2010 4:14 PM To: Valantou Grover Cc: histonet@lists.utsouthwestern.edu Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! The email you received is a Nigerian Scam. Someone was able to hack into Jerry's computer and get all his contact list! You happened to be one of his contacts. The same sort of Scam happened to me last October. Fortunately for me, I didn't have any bank account information in my computer. I have a MAC and so I went to the Apple Genius Bar and they checked my computer for any viruses. Fortunately, it is very difficult to get into a MAC. This has been on the news in Idaho and various other states. DO NOT RESPOND to any emails that want any personal information!!! You need to clean-up your computer from any viruses. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: I am just curious, can someone check on Jerry to see if he is dead or alive, or if someone stole his phone/laptop to get this message to me. I remember the email address from Florida Society for Histotechnology, I am wondering if this is real or if someone is assuming his identity overseas, foul play, etc. Valantou Grover, Ph.D. HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 ** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 >>>
[Histonet] Sectioning problem
I am working on an old microtome, a Reichert Histostat with a Tissue-Tek II microtome chuck adapter, and the last time I had this machine cleaned and rehabbed by my repairman, he said that the springs were shot on the chuck adapter, resulting in the windowblind chatter that I was getting in my sections. Unfortunately he did not have a replacement, Sakura doesn't make or sell them anymore, and the one source for replacement adapters I did find online sold me a part that had the same problem-shot springs (I assume this, as I was getting the same chatter with the "new" part). I have Googled, searched on eBay, checked online used scientific/medical equipment suppliers to no avail...but I will keep looking in case something comes up. So I guess my question is...would one of the newer cassette holders fit in my microtomes? In addition to the Reichert mentioned above, I also have an American Optical 860. Or is it possible to figure out how to replace the springs in the thing? (I have half a mind to wander over to the mechanical engineering dept. here to ask that question, but I figured I'd start here on Histonet.) I would really hate to have to replace the microtomes just because I can't get a cassette holder for them. Thanks for all your help, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
To all, OK, looks like most of you are saying the same thing-work fast! :o) I've printed out all of your replies and discussed them with the graduate student who needs this done for her thesis, and she agrees with me-it's just going to take practice. I'll try the "cool slowly/work fast" suggestions first and see how that goes, then see about the trick with the cucumber (which sounds really cool!), slowly edging the mold onto the cold plate as I go, and using sponges to help flip the samples on end (and not necessarily in that order). I don't want to try too many things at once, lest I drive myself nuts. The first batch of samples should be coming sometime next week. Wish me luck, and thanks for all your help! Kathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding multiple GI pieces on end in a paraffin block
Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific
Just saw this email now. Here it is: http://www.fishersci.com/wps/portal/PRODUCTDETAIL?prodcutdetail=%27prod%27&productId=5001566&catalogId=29103&matchedCatNo=22050112&pos=7&catCode=HC_SC&endecaSearchQuery=%23store%3DHealthCare%23N%3D0%23rpp%3D15&fromCat=yes&keepSessionSearchOutPut=true&fromSearch=Y&searchKey=richards||hematoxylin||Allan||Richard&highlightProductsItemsFlag=Y Hope this helps, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific, > after > all they own RA? I am helping put together a supply list for > laboratory, > and am having trouble finding this on Thermo Scientific website. I did > not > see it on the Fisher Heathcare site either. Personally, and I hate to > complain, but I am finding Thermo's website a nightmare to navigate. I > may > have to go to VWR and Cardinal who seem to have it, then why not Thermo? > > > > Impatiently, > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] unsubscribe
Sorry about that, I hit the Send key prematurely. Now: Click on this link that is at the bottom of EVERY Histonet email: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Scroll down to the bottom of the page and follow the instructions to unsubscribe yourself. Really, the webmaster should change that line to "If you want to unsubscribe, click here.", with the link attached to the word "here". Thank you! Kathy Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Please could you unsubscribe me? > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] unsubscribe
Sara and everybody else: PLEASE PAY ATTENTION. > Please could you unsubscribe me? > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
