[Histonet] Amyloid A controls

[Dooley, Elaine via Histonet]

Hello Histonetters,

I am looking for a good "Amyloid A control".  We have an anti-amyloid A 
antibody which is specific only for amyloid A.  We have tried our commercial 
congo red controls and they do not work.  We have only had a few positive 
cases.  I am willing to trade control blocks.  We have parvovirus, h,pylori,  
C4d on heart, and many other controls just ask and maybe we could make a trade.

Elaine Dooley

Shands Labs at Rocky Point
4800 SW 35th drive
Room 1130 Histology
Gainesville FL 32608

352-265-0111   ext 72117


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Re: [Histonet] Amyloid by Congo Red

[Bryan Llewellyn]

This is a well known phenomenon of amyloid staining and it is fairly 
common. It is due to the fact that amyloid is a very variable material 
and different samples may react differently to the dyes. I suggest you 
restain using Highman's congo red method 
(http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm) is 
more reliable than Bennhold's. I always found it the more reliable. If 
it is still unstained, try a sirius red method (using CI 35780, sirius 
red F3B, NOT sirius red 4B). You might also want to consider thioflavine 
T and the other methods using dyes other than direct cotton dyes.


Read the article, which includes a list of methods, at: 
http://stainsfile.info/StainsFile/stain/amyloid/amyloid.htm


Bryan Llewellyn


Jeffrey Robinson wrote:

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  
We are cutting our slides and controls at 9 and staining in Congo Red for 1 
hour.  The control stains fine but the patient tissue is staining negative even 
on cases that the pathologist assures us should be positive for amyloid.  We 
are using the Leica APEX charged slides with control and patient tissue on the 
same slide.  Does anyone have any thoughts on why the patient tissue is not 
staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


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RE: [Histonet] Amyloid by Congo Red

[Lewis, Sarah]

We do all of our Congo Red staining on Muscle and Nerve frozen sections.  
Sometimes when there is very little amyloid detected using the 
bright-field/polarizing microscope our pathologist will confirm under a 
fluorescent microscope with the Rhodamine Red filter.  This shows the amyloid 
inclusions very well. 


Sarah E. Lewis HT, ASCP
The Research Institute at
Nationwide Children's Hospital
Center for Gene Therapy
Neuromuscular Division
Rm WA3110
(614)722-2204




-Original Message-
From: Jeffrey Robinson [mailto:jrobin...@pathology-associates.com] 
Sent: Friday, January 23, 2015 12:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid by Congo Red

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  
We are cutting our slides and controls at 9 and staining in Congo Red for 1 
hour.  The control stains fine but the patient tissue is staining negative even 
on cases that the pathologist assures us should be positive for amyloid.  We 
are using the Leica APEX charged slides with control and patient tissue on the 
same slide.  Does anyone have any thoughts on why the patient tissue is not 
staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


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attachments. Thank you.


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Re: [Histonet] Amyloid by Congo Red

[Michael Ann Jones]

What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 1/23/15, 10:43 AM, "Jeffrey Robinson"
 wrote:

>Greetings to all Histotechs-  Here's an amyloid question for the
>braintrust.  We are cutting our slides and controls at 9 and staining in
>Congo Red for 1 hour.  The control stains fine but the patient tissue is
>staining negative even on cases that the pathologist assures us should be
>positive for amyloid.  We are using the Leica APEX charged slides with
>control and patient tissue on the same slide.  Does anyone have any
>thoughts on why the patient tissue is not staining?  Thanks!
>
>Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab,
>Clovis, CA.
>
>
>This email and attachments may contain PHI that is privileged and
>confidential and is not intended for any unauthorized person. If you, the
>reader, are not the intended recipient you are hereby notified that any
>dissemination, distribution or copying of this communication is strictly
>prohibited. Do not read the email but instead reply to the sender and
>destroy the message and any attachments. Thank you.
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[Histonet] Amyloid by Congo Red

[Jeffrey Robinson]

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  
We are cutting our slides and controls at 9 and staining in Congo Red for 1 
hour.  The control stains fine but the patient tissue is staining negative even 
on cases that the pathologist assures us should be positive for amyloid.  We 
are using the Leica APEX charged slides with control and patient tissue on the 
same slide.  Does anyone have any thoughts on why the patient tissue is not 
staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


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[Histonet] Amyloid Red

[Webb, Dorothy L]

Does anyone out there use the Amyloid Red stain from Anatech in place of the 
Congo Red dye?  Due to the pricing, would like some critiques before I order 
it!!!

Thanks,

Dorothy Webb



  
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Re: [Histonet] Amyloid Precursor Protein (APP) by IHC

[Jan Shivers]

Carole,
This may or may not be of help - Millipore's xAPP (MAB348) works on horses;
HIER using high pH retrieval solution.  I don't have data on reactivity on
pigs yet.

Good luck,
Jan Shivers
UMN Vet Diag Lab
St. Paul, MN


On Mon, Sep 16, 2013 at 3:52 PM, Johnson, Carole wrote:

> Hello All,
>
> Does anyone out there in Histoland have experience with this antibody? I
> have been asked to look into running IHC on pig brain on the Bond Max
> platform. I am looking into the antibodies from LS Bio and
> Invitrogen/LifeSciences, and would appreciate any insight you could
> provide. Thanks in advance for your help! If you prefer, you may reply
> privately at the email below.
>
> Carole Johnson, HT(ASCP)cm
> Histopathology Section Supervisor
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
>
> cjohn...@nmda.nmsu.edu
>
>
>
>
> Confidentiality Notice: New Mexico has a very broad public records law.
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> Your e-mail communications may therefore be subject to public disclosure.
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[Histonet] Amyloid Precursor Protein (APP) by IHC

[Johnson, Carole]

Hello All,

Does anyone out there in Histoland have experience with this antibody? I have 
been asked to look into running IHC on pig brain on the Bond Max platform. I am 
looking into the antibodies from LS Bio and Invitrogen/LifeSciences, and would 
appreciate any insight you could provide. Thanks in advance for your help! If 
you prefer, you may reply privately at the email below.

Carole Johnson, HT(ASCP)cm
Histopathology Section Supervisor
New Mexico Department of Agriculture
Veterinary Diagnostic Services

cjohn...@nmda.nmsu.edu




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Re: [Histonet] amyloid stain

[Rene J Buesa]

Check/change the solution
René J.

From: Gale Limron 
To: "histonet@lists.utsouthwestern.edu"  
Sent: Thursday, May 30, 2013 11:26 AM
Subject: [Histonet] amyloid stain



Hello,
It's been awhile since we ran an amyloid stain.Today, using Richard 
Allan Chromaview kit, our staining tech noticed a lot of precipitant in the 
working Congo Red solution. Any thoughts or advice?
Thanks,
Gale

Gale Limron, HT, CT (ASCP)
Union Hospital
Dover, Oh



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[Histonet] amyloid stain

[Gale Limron]

Hello,
 It's been awhile since we ran an amyloid stain.Today, using 
Richard Allan Chromaview kit, our staining tech noticed a lot of precipitant in 
the working Congo Red solution. Any thoughts or advice?
Thanks,
Gale

Gale Limron, HT, CT (ASCP)
Union Hospital
Dover, Oh



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[Histonet] Amyloid

[Cesar Francisco Romero]

I think this is a great article to read about amyloid. This is the link to the 
paper 
http://propath.com/newsletters-immunohistochemistry-173/181-september-2004-immunohistochemistry-in-amyloidosis
 It belongs to a great Pathologist Dr. Rodney T. Miller

Dr. César RomeroBuenos AiresArgentina 
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RE: [Histonet] Amyloid control tissue

[Britton, Josette C]

American Master Tech 800-860-4073

Josie Britton HT QIHC (ASCP)
Cheshire Medical Center
Keene, NH

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Moe,
Barbi A
Sent: Monday, February 04, 2013 2:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid control tissue

Does anyone know where I could purchase (or trade for) a block of
amyloid positive tissue?



Alternatively, for those who purchase ready-cut amyloid control slides,
could you share what vendor you have been happy with?



Thanks in advance!



Barb Moe

Gundersen Lutheran Medical Center

La Crosse WI

ba...@gundluth.org<mailto:ba...@gundluth.org>
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[Histonet] Amyloid control tissue

[Moe, Barbi A]

Does anyone know where I could purchase (or trade for) a block of amyloid 
positive tissue?



Alternatively, for those who purchase ready-cut amyloid control slides, could 
you share what vendor you have been happy with?



Thanks in advance!



Barb Moe

Gundersen Lutheran Medical Center

La Crosse WI

ba...@gundluth.org
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[Histonet] Amyloid stain

[Roberta Horner]

Does anyone have a procedure to do an amyloid stain on a smear?  Someone at the 
university wants to see if there is amyloid in milk.
Roberta Horner HT/HTl
Animal Diagnostic Lab
Penn State University
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Re: [Histonet] amyloid ? for Bob Richmond

[Richard Cartun]

Yes, insulinomas can show amyloid; however, they are very rare tumors.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Carrie Disbrow"  4/6/2010 5:05 PM >>>


Hi,
I was also told or read somewhere  that insulinomas are good amyloid controls. 
I've never had a chance to test any blocks from these cases. What has your 
experience been? Thanks for  your help.
Carrie
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[Histonet] amyloid ? for Bob Richmond

[Carrie Disbrow]

Hi,
I was also told or read somewhere  that insulinomas are good amyloid controls. 
I've never had a chance to test any blocks from these cases. What has your 
experience been? Thanks for  your help.
Carrie
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Re: [Histonet] Amyloid Stain

[Richard Cartun]

I find IHC for amyloid A (AA) helpful only in kidney; however, it is not 
requested often.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Drew Meyer <41dm...@gmail.com> 4/5/2010 2:37 PM >>>
Hey Guys,

I was hoping to get some help with the best way to approach a request for an
amyloid stain.  The only thing I've ever done in the past is the traditional
Congo Red... however we were wondering how the Amyloid A Immuno stain is.
Our pathologists were wondering if the Amyloid A is more/less reliable and
if there are any limitations associated with this antibody.  I just don't
know that much about it and was hoping you guys could enlighten me!  Thanks
for the help!

Drew
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RE: [Histonet] Amyloid Stain

[Sebree Linda A]

Drew,

At one time we were going to bring this stain in-house (Amyloid A) but
finding positive control material was problematic.  One of our
pathologists remembered a case from more than 20 years ago (and had
documented it!) but it was still just a tiny renal biopsy.  We found
that ProPath (214-237-1894, www.propath.com ) does this antibody so we
gave them some of our renal biopsy and since then have sent our cases to
them for staining.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew
Meyer
Sent: Monday, April 05, 2010 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid Stain

Hey Guys,

I was hoping to get some help with the best way to approach a request
for an amyloid stain.  The only thing I've ever done in the past is the
traditional Congo Red... however we were wondering how the Amyloid A
Immuno stain is.
Our pathologists were wondering if the Amyloid A is more/less reliable
and if there are any limitations associated with this antibody.  I just
don't know that much about it and was hoping you guys could enlighten
me!  Thanks for the help!

Drew
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[Histonet] Amyloid Stain

[Drew Meyer]

Hey Guys,

I was hoping to get some help with the best way to approach a request for an
amyloid stain.  The only thing I've ever done in the past is the traditional
Congo Red... however we were wondering how the Amyloid A Immuno stain is.
Our pathologists were wondering if the Amyloid A is more/less reliable and
if there are any limitations associated with this antibody.  I just don't
know that much about it and was hoping you guys could enlighten me!  Thanks
for the help!

Drew
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Re: [Histonet] Amyloid staining on frozen muscle bx's

[Bryan Llewellyn]

The method at the address below should work on cryostat sections.

http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm

Bryan Llewellyn


- Original Message - 
From: "Tony Henwood" 
To: "Sharon Allen" ; 


Sent: Wednesday, August 19, 2009 3:26 PM
Subject: RE: [Histonet] Amyloid staining on frozen muscle bx's


Sharon,

For successful amyloid staining with Congo red, formalin fixation might
be required.
I have not been able to find any references for this except that
formalin is used in the demonstration of amyloid in fat aspirations
(reference: Duston et al (1987) Am J Med 82:412-414)

Have you tried one of the metachromatic dyes (eg Crystal Violet)?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Thursday, 20 August 2009 6:57 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Amyloid staining on frozen muscle bx's


Hi,
What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca

*
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RE: [Histonet] Amyloid staining on frozen muscle bx's

[Tony Henwood]

Sharon,

For successful amyloid staining with Congo red, formalin fixation might
be required.
I have not been able to find any references for this except that
formalin is used in the demonstration of amyloid in fat aspirations
(reference: Duston et al (1987) Am J Med 82:412-414)

Have you tried one of the metachromatic dyes (eg Crystal Violet)?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Thursday, 20 August 2009 6:57 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Amyloid staining on frozen muscle bx's


Hi,
What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca

*
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solely for the use of the individual or entity to whom they are addressed. If 
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[Histonet] Amyloid staining on frozen muscle bx's

[Sharon Allen]

Hi,
What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success.
Thanks
Sharon
sal...@hsc.mb.ca
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[Histonet] Amyloid A- IHC stain

[Cazares, Ruth]

Hello fellow Histonetters,

I was wondering if anyone is running Biocare's Amyloid A or Amyloid P 
antibodies on Ventana's Benchmark. If so, are you using the concentrated or 
predilute antibody?

Also, are you running these alone or with a Congo Red stain (as in a panel)? 
Any information is greatly appreciated.

Thank you,

Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

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[Histonet] Amyloid control tissue

[Cheri Miller]

Does anyone have any Amyloid tissue they are willing to share?? Thanks,
Cheri

 

Cheryl Miller HT (ASCP)

Histology Supervisor

Physicians Laboratory,P.C.

Omaha, Ne. 

402 738 5052

 



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[Histonet] Amyloid Controls

[Beth Delescavage]

Hi Everyone~
We are having a hard time finding Amyloid controls.  Can anyone
recommend a company that has good controls?  

Thanks~
Beth 

Beth Delescavage, BS, HTL (ASCP) QIHC


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RE: [Histonet] Amyloid

[Mark A Burton]

Dorothy,

We've done several comparisons like this for amyloid staining. In our
experience, 8um works fine. We did see stronger staining (birefringence) in
some thicker sections with Congo Red but I don't think there would be a
significant improvement in staining (sensitivity?) from 8um to 10um. I
wouldn't cut sections any thinner than 8um but you can still get amyloid
staining at 4um. It would be relatively easy to take your controls and cut
them different thicknesses just to prove the point. Good luck!


Mark A Burton HTL ASCP
Lab Manager & Sr. Histotechnologist
Molecular Aging & Development Lab
Boston University Medical Campus

 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, September 16, 2008 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid

We have been cutting our tissue for amyloid staining @ 8 microns.  One
of my pathologists heard that 10 microns is now standard  and to use a
negative and weakly positive control.  Does anyone have any new
information in this area?  Thanks ahead of time!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care

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Re: [Histonet] Amyloid

[Maxim_71]

Dorothy:
We does Highman's method (Bancroft&Stevens, 1977 p.164)
Both 8 and 10 microns give good results.
Maxim Peshkov
Russia,
Taganrog.
 mailto:[EMAIL PROTECTED]


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Re: [Histonet] Amyloid

[Rene J Buesa]

No, but you should ask your pathologist where that "standard" comes from. S/he 
should know since s/he brought the issue up.
René J.

--- On Tue, 9/16/08, Webb, Dorothy L <[EMAIL PROTECTED]> wrote:

From: Webb, Dorothy L <[EMAIL PROTECTED]>
Subject: [Histonet] Amyloid
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 16, 2008, 11:16 AM

We have been cutting our tissue for amyloid staining @ 8 microns.  One
of my pathologists heard that 10 microns is now standard  and to use a
negative and weakly positive control.  Does anyone have any new
information in this area?  Thanks ahead of time!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care

This e-mail and any files transmitted with it are confidential and are intended
solely for the use of the individual or entity to whom they are addressed. If
you are not the intended recipient or the individual responsible for delivering
the e-mail to the intended recipient, please be advised that you have received
this e-mail in error and that any use, dissemination, forwarding, printing, or
copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the
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Re: [Histonet] Amyloid

[Rene J Buesa]

No, but you should ask your pathologist where that "standard" comes from. S/he 
should know since s/he brought the issue up.
René J.

--- On Tue, 9/16/08, Webb, Dorothy L <[EMAIL PROTECTED]> wrote:

From: Webb, Dorothy L <[EMAIL PROTECTED]>
Subject: [Histonet] Amyloid
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 16, 2008, 11:16 AM

We have been cutting our tissue for amyloid staining @ 8 microns.  One
of my pathologists heard that 10 microns is now standard  and to use a
negative and weakly positive control.  Does anyone have any new
information in this area?  Thanks ahead of time!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care

This e-mail and any files transmitted with it are confidential and are intended
solely for the use of the individual or entity to whom they are addressed. If
you are not the intended recipient or the individual responsible for delivering
the e-mail to the intended recipient, please be advised that you have received
this e-mail in error and that any use, dissemination, forwarding, printing, or
copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the
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[Histonet] Amyloid

[Webb, Dorothy L]

We have been cutting our tissue for amyloid staining @ 8 microns.  One
of my pathologists heard that 10 microns is now standard  and to use a
negative and weakly positive control.  Does anyone have any new
information in this area?  Thanks ahead of time!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care

This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient or the individual responsible for delivering 
the e-mail to the intended recipient, please be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, or 
copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the 
HealthPartners Support Center by telephone at (952) 967-6600. You will be 
reimbursed for reasonable costs incurred in notifying us.
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