Re: [Histonet] Anti-human abs work on porcine tissue
This is great FYI, Jan. Our lab works a lot with pig tissues, too. And we, too, have found in our lab that a number of human Abs appear to work on pig tissues. Still, I can definitely appreciate the difficulties in finding a pig-specific antibody, dealing the unknowns of cross-reactivity and degree of homology. Jan, I will certainly keep you in mind when we are seaching for a porcine-reacting antibody! Thanks for sharing that. Regards, Merced --On Thursday, March 18, 2010 7:48 AM -0500 Jan Shivers wrote: I work on all species, porcine being one of them. I've never done a % survey of the number of Abs made against human epitopes that also work on porcine tissue, but I can give you generalizations. All of the rabbit polyclonal antibodies that I've tried have cross-reacted with porcine tissue. The polyclonals that I use the most are the Abs against hormones, neuroendocrine markers, vasculature, astrocytes, etc. Most of the mouse monoclonal antibodies that I've tried have cross-reacted with porcine tissue. These would be the intermediate filaments, melanoma antigens, cell proliferating antigens, neuroendocrine markers, etc. You may have more difficulty with leukocyte markers (CD markers). They seem to have less homology with human markers than the above items, and not all cross-react, in my experience. You only learn this by trial and error, unfortunately, since data about species cross-reactivity is rare. I have 2 polyclonal and 2 monoclonal CD markers that cross-react with porcine. That is not to say that there aren't more, but that's all that I currently have worked up on porcine tissue. I have not tried Abs like ER, PR, CEA, TTF, etc. I've done species cross-reactivity studies with every antibody I have in stock (>80), so if you send me a list of the antibodies you're interested in (directly to me), I can let you know which ones cross-react with porcine tissue, and which vendor works for me. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "RICKY MATHIS" To: "histonet" Sent: Wednesday, March 17, 2010 8:15 PM Subject: [Histonet] Anti-human abs work on porcine tissue I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same "we did not try it on pig tissue" response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anti-human abs work on porcine tissue
I work on all species, porcine being one of them. I've never done a % survey of the number of Abs made against human epitopes that also work on porcine tissue, but I can give you generalizations. All of the rabbit polyclonal antibodies that I've tried have cross-reacted with porcine tissue. The polyclonals that I use the most are the Abs against hormones, neuroendocrine markers, vasculature, astrocytes, etc. Most of the mouse monoclonal antibodies that I've tried have cross-reacted with porcine tissue. These would be the intermediate filaments, melanoma antigens, cell proliferating antigens, neuroendocrine markers, etc. You may have more difficulty with leukocyte markers (CD markers). They seem to have less homology with human markers than the above items, and not all cross-react, in my experience. You only learn this by trial and error, unfortunately, since data about species cross-reactivity is rare. I have 2 polyclonal and 2 monoclonal CD markers that cross-react with porcine. That is not to say that there aren't more, but that's all that I currently have worked up on porcine tissue. I have not tried Abs like ER, PR, CEA, TTF, etc. I've done species cross-reactivity studies with every antibody I have in stock (>80), so if you send me a list of the antibodies you're interested in (directly to me), I can let you know which ones cross-react with porcine tissue, and which vendor works for me. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "RICKY MATHIS" To: "histonet" Sent: Wednesday, March 17, 2010 8:15 PM Subject: [Histonet] Anti-human abs work on porcine tissue I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same "we did not try it on pig tissue" response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anti-human abs work on porcine tissue
I work on porcine tissue regularly and I can't confirm the 10%. Maybe I accidentially used only the highly conserved proteins, I didn't check on that. But I usually try anti-human antibodies and most of the time they work really well! Alexandra Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.me...@trauma.lbg.ac.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anti-human abs work on porcine tissue
I routine use anti-human antibodies to stain mouse tissue, so it can be done. It completely depends on the antigen they used to immunize. If the human and pig proteins are highly homologous or identical, then it has a good chance of working. If they're not, you're in uncharted territories. If you ask nicely, most companies will tell you what antigen or antigen fragment they used, but most don't actively advertise this. Adam On Wed, Mar 17, 2010 at 8:15 PM, RICKY MATHIS wrote: > I work with some porcine tissues and it is sometimes difficult to find > antibodies specific to pig. A doctor I work with asked some one he knew in > Europe about using antibodies that are listed as working in human being used > on porcine tissue. This person stated that there would be about a 10% > chance that the anti-human antibodies would work on porcine tissues. The > antibody companies mostly give the same "we did not try it on pig tissue" > response. I understand that it is likely expensive to continue to test > antibodies on a wide variety of animal tissues, so that is fine. But what > do you guys think about the 10% chance? I would have said more than that. > Thank you in advance for your time, > Cathy > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anti-human abs work on porcine tissue
I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same "we did not try it on pig tissue" response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
