Re: [Histonet] Beta-Amyloid antibody for IHC
If you use clone 6E10 it will demonstrate beta Amyloid only, after Formic acid AR After Citric acid HIER, APP will also be demonstrated Imho Check out Biosource ( Invitrogen) 44-344 and 44-348? Something about x-reactivity between Abeta 1-42 and Abeta 1-43? Or not. I'd be interested to know if this is valid Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Beta-Amyloid antibody for IHC
You are welcomeit should be the original one by SIgnet...then COvancenow BioLEgends C On Wed, Dec 27, 2017 at 3:34 PM, Cartun, Richard < richard.car...@hhchealth.org> wrote: > No, I would like to use “6E10” if it’s still available. Thank you! > > > > *Richard* > > > > *From:* Colleen Forster [mailto:cfors...@umn.edu] > *Sent:* Wednesday, December 27, 2017 4:23 PM > *To:* Cartun, Richard > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC > > > > CAUTION: This email is from outside HHC. USE CARE when opening attachments > or links. > > Do you knot want to use 6E10 anymore? I believe you can still get it from > Biolegends? > > https://www.biolegend.com/en-us/products/purified-anti- > beta-amyloid--1-16-antibody-11228 > > Respectfully, > > Colleen Forster HT(ASCP)QIHC > > *University of Minnesota* > > > > On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > We are looking for a good antibody to detect Beta-Amyloid in > formalin-fixed, paraffin-embedded human brain tissue. Any > recommendations? We have been using the clone "6E10" which we purchased > from Signet Laboratories back in 2001. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > <https://maps.google.com/?q=80+Seymour+Street+%0D+Hartford,+CT%C2%A0+06102&entry=gmail&source=g> > Hartford, CT 06102 > <https://maps.google.com/?q=80+Seymour+Street+%0D+Hartford,+CT%C2%A0+06102&entry=gmail&source=g> > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > *Reminder: This e-mail and any attachments are subject to the current HHC > email retention policies. Please save or store appropriately in accordance > with policy. * > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Beta-Amyloid antibody for IHC
No, I would like to use “6E10” if it’s still available. Thank you! Richard From: Colleen Forster [mailto:cfors...@umn.edu] Sent: Wednesday, December 27, 2017 4:23 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Beta-Amyloid antibody for IHC CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC University of Minnesota On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Beta-Amyloid antibody for IHC
We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Beta-Amyloid antibody for IHC
Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC *University of Minnesota* On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet < histonet@lists.utsouthwestern.edu> wrote: > We are looking for a good antibody to detect Beta-Amyloid in > formalin-fixed, paraffin-embedded human brain tissue. Any > recommendations? We have been using the clone "6E10" which we purchased > from Signet Laboratories back in 2001. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
