Re: [Histonet] CD68 and CD204 for dogs/pigs
Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I need > them to work on dogs and pigs, but of course, there's not many vendors who > list species cross-reactivities on their data sheets beyond the usual human > and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have success > using it on dog tissue. Am looking for a different clone that will > cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and porcine, > > not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > l...@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and > pigs > > (on FFPE tissue)? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN 55108 > > 612-624-7297 > > shive...@umn.edu > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual human and mouse. In my earlier trials years ago with CD68 (clone KP1), I didn't have success using it on dog tissue. Am looking for a different clone that will cross-react with dogs. Jan On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala wrote: > Jan > > MAC387 is a marker that we have used successfully in canine and porcine, > not entirely specific to macs but may work for your situation. > > LIz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > Sent: Tuesday, February 24, 2015 3:59 PM > To: histonet > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs > (on FFPE tissue)? I don't seem to find much information on species > cross-reactivity online. > > Thanks much in advance. > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hi, MAC387 is a great marker but it detects less macrophages than CD68. In our experience (sheep and cattle), MAC387 is good for labelling macrophages that have recently reach the lesion, while those resident or long standing macrophages would be negative to MAC387 (but mostly positive to CD68, which is a more general marker for macs). No idea about CD204 though. Just in case this helps Regards Julio On 25/02/2015 18:08, Elizabeth Chlipala wrote: Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 and CD204 for dogs/pigs
I just received Cd204 from Biorbyt but have not validated a protocol. They are located in the UK but have a distributor in San Francisco so shipping is not outrageous. References to its application: The class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma. J Comp Pathol 2013 Feb 16;148(2-3):188-96. Epub 2012 Aug 16. Y Kato, M Murakami, Y Hoshino, T Mori, K Maruo, A Hirata, T L D R Nakagawa, T Yanai, H Sakaiand Immunocytochemical detection of the class A macrophage scavenger receptor CD204 using air-dried cytologic smears of canine histiocytic sarcoma Yuki Kato1, Risa Funato1, Akihiro Hirata, Mami Murakami, Takashi Mori, Koji Maruo, Tokuma Yanai and Hiroki Sakai Article first published online: 28 AUG 2014 If you or anyone has had success with this antibody, please let me know. Thanks, Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 and CD204 for dogs/pigs
Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68 and CD204 for dogs/pigs
Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
We use MAC387 not exactly the same as CD68 but stains macrophages, monocytes and I think granulocytes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia [reuel.corne...@tsrh.org] Sent: Monday, August 18, 2014 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 Does anybody out there knows a CD68 antibody that works with pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa Cruz, Dako and Novus that they say works with pig tissue but it did not. We tried using heat antigen retrieval and enzyme digestion for paraffin, it did not work. We also use pig frozen tissue and still do not work. Any suggestion. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68
Does anybody out there knows a CD68 antibody that works with pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa Cruz, Dako and Novus that they say works with pig tissue but it did not. We tried using heat antigen retrieval and enzyme digestion for paraffin, it did not work. We also use pig frozen tissue and still do not work. Any suggestion. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 or F4/80
We had success with CD68 on mouse tissue from Boster cat# PA1518. For F4/80 we use the rat anti-mouse antibody from eBioscience cat# 14-4801. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mesru T Sent: Wednesday, February 12, 2014 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 or F4/80 Hi Anna, Your subject line is F4/80 and you are asking for CD68. I thought they are different antibodies, but I may be wrong. We have never been able to find CD68 ab that works well of FFPE mouse tissues. Recently we had success with F4/80 Rat anti-mouse Clone: BM8 from BioLegend. It only worked with citric buffer pH6 100C 10min. We also use Iba-1 as macrophage marker on mouse FFPE tissues. If anyone has good CD68 on FFPE mouse tissues please could you share your experiences? It will be of great help. Hope this helps. Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68 or F4/80
Hi Anna, Your subject line is F4/80 and you are asking for CD68. I thought they are different antibodies, but I may be wrong. We have never been able to find CD68 ab that works well of FFPE mouse tissues. Recently we had success with F4/80 Rat anti-mouse Clone: BM8 from BioLegend. It only worked with citric buffer pH6 100C 10min. We also use Iba-1 as macrophage marker on mouse FFPE tissues. If anyone has good CD68 on FFPE mouse tissues please could you share your experiences? It will be of great help. Hope this helps. Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68
Good Morning! CD68 (for the BOND)is backordered until May and I am looking for recommendations - Cellmarque or Biocare? Is there something better? I appreciate your input. Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
I use both KP-1 and PGM-1. KP-1 is ordered about ten times as much as the PGM-1. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68??`
Another vote for KP-1. It also stains mast cells. Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68
I use the ED1 from abdserotec and it works perfectly can dilute up to 1/500 to 1/1000 2010/4/30 Cynthia Pyse > Happy Friday Everyone > > What clone is everyone using for the CD68 antibody for FFPE human tissue? > Thanks for the info in advance. Everyone have a great weekend. > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cp...@x-celllab.com > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
We are currently using KP-1 and HAM 56 Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA >>> Drew Sally A 04/30/10 12:11 PM >>> We also use KP1 Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
We also use KP1 Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68
Hi Cindy, My lab uses KP-1. Mark On Fri, Apr 30, 2010 at 8:48 AM, Cynthia Pyse wrote: > Happy Friday Everyone > > What clone is everyone using for the CD68 antibody for FFPE human tissue? > Thanks for the info in advance. Everyone have a great weekend. > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cp...@x-celllab.com > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68
Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 on FFPE human tissue
Yes, we too. Pretreatment with trypsin. Van: histonet-boun...@lists.utsouthwestern.edu namens Richard . Verzonden: wo 18-11-2009 15:09 Aan: Kim Merriam CC: Histonet Onderwerp: Re: [Histonet] CD68 on FFPE human tissue PG-M1 from dako 2009/11/18 Kim Merriam > What antibodies are people using for this marker? > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 on FFPE human tissue
PG-M1 from dako 2009/11/18 Kim Merriam > What antibodies are people using for this marker? > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68 on FFPE human tissue
What antibodies are people using for this marker? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 counts
Well, you're going to have dendritic cells staining with CD68. Dendritic cells be really large, full of cytoplasm, and multinucleated. I guess that brings you back to "hopefully macrophages". Maybe someone can recommend a more specific marker or better criteria for what a macrophage is... Mark On Wed, Jan 21, 2009 at 8:48 AM, Edwards, R.E. wrote: > > I am attempting to accurately assess the number of CD 68 positive cells > (hopefully macrophages) in biopsies, they have been GMA processed, ABC > staining, with AEC visualisation; no problem with a nucleated cell with > positive cell membrane, but they are in the minority, so the question > how does one judge the cut-off point that makes a positive cell into a > negative cell if you catch my drift??. I am quite experienced at counting > cells but feel that obtaining reliable, consistent results from this > material is quite challenging. > All ideas/thoughts gratefully received > > Cheers >Richard Edwards > University of Leicester >U.K. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68 counts
I am attempting to accurately assess the number of CD 68 positive cells (hopefully macrophages) in biopsies, they have been GMA processed, ABC staining, with AEC visualisation; no problem with a nucleated cell with positive cell membrane, but they are in the minority, so the question how does one judge the cut-off point that makes a positive cell into a negative cell if you catch my drift??. I am quite experienced at counting cells but feel that obtaining reliable, consistent results from this material is quite challenging. All ideas/thoughts gratefully received Cheers Richard Edwards University of Leicester U.K. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
