RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs
Patsy How do you order that rat anti-mouse CD31. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, April 10, 2010 10:40 AM To: 'Jan Shivers'; 'Jean-Martin Lapointe'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs Yea you can use F8 and SMA for vessel lining cells but those will not pick up all the developing endothelial cells like cd31 or cd34. There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig, I will try it and see, it works great on mouse tissue ffpe. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Friday, April 09, 2010 10:24 AM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Québec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs
Yea you can use F8 and SMA for vessel lining cells but those will not pick up all the developing endothelial cells like cd31 or cd34. There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig, I will try it and see, it works great on mouse tissue ffpe. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Friday, April 09, 2010 10:24 AM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Québec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cd31 in pigs
Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Québec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cd31 in pigs
Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Québec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cd31 in pigs.
Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet