[Histonet] Cryostat cleaning
The only method acceptable to CAP is bringing the cryostat to room temp and disinfecting weekly UV light will not be an acceptable alternative by CAP. We clean out all tissue debris, wipe down with abs alcohol, bring cryostat to room temp, use Cavicide to disinfect, wipe down again with abs alcohol. We use 70 % alcohol for daily cleaning. Cindy Baranowski Emory Healthare Atlanta, GA Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat cleaning
We wipe out the excess tisue( if there is any), wipe down the chamber with abs. Alcohol then spray the chamber with "CaviCide", let it sit for @ 3-5 minutes and then wipe the chamber out again. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, August 16, 2013 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat cleaning
Ann, There are many tuberculocidal cleaners. Just search for it. If you are in a hospital the central supply will most likely have some in stock. We use PD-128 by Spartan Chemical http://www.spartanchemical.com/products/product/101604 Here are some references for cryostat cleaning: Cryostat decontamination references: College of American Pathologists, Commentary on certification questionnaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 NCCLS site: http://www.nccls.org/ Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken UCSF Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, August 16, 2013 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat cleaning
ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Cleaning
Ann, Cryostat decontamination references: College of American Pathologists, Commentary on certification questionaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 http://www.cap.org/HTML/checklist_html/cklst08p.html NCCLS site: http://www.nccls.org/ Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, August 06, 2013 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Cleaning Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Cleaning
Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
