RE: [Histonet] Decal Solution for Bone Marrows
I use 5% formic acid, you can make it or purchase Immunocal from Decal Chemicals, it is slower than the rapid decal solutions but much better for IHC and FE (although we used to have to do Fe stains on the smears or clot because besides the decal, iron can be washed out in tissue processing). Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. B ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decal Solution for Bone Marrows
I neglected to say that the iron stain does not work well on decaled specimens. We perform iron stains on bone marrow clots and smears . In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time, trathbo...@somerset-healthcare.com writes: Decalcifier II from Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decal Solution for Bone Marrows
We use the IEC from Biocare. The bone biopsies are fixed for a minimum of 2 hours and decaled in the IEC for two hours. Sections great and immunohistochemistry stains are beautiful. In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time, trathbo...@somerset-healthcare.com writes: Decalcifier II from Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Decal Solution for Bone Marrows
Decalcifier II from Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decal Solution for Bone Marrows
I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
