Re: [Histonet] Delays in tissue embedding after mouse tissue

[Hobbs, Carl via Histonet]

Hi Jamie
I do this often, for he same reason
Ms and rat soft organs ( hollow and solid) leg bones, whole ankles, ears, cube 
of tissue containing cochlea, chick ribs.
Once  the delay was 6 months. I allowed the cassettes with tissue in to 
solidify ( as do you) and then leave them in a labelled box to await embedding.
Sometimes the researcher cannot embed at scheduled time and I would rather fix 
then process than have fixed tissues sitting in 70% alcohol until they can be 
processed and embedded on schedule ( sure, it doesn't adversely affect, in my 
xp, to leave in 70% alc at 4C provided the tissues have been optimally fixed ( 
meaning in 10% Formalin)

Obviously I strongly discourage any delay, telling them that it is deleterious
Gotta keep them Researchers in order
;-)
Best wishes
Carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6813
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[Histonet] Delays in tissue embedding after mouse tissue processing

[Erickson, Jamie E via Histonet]

Hello All,
I have a question about effects on tissues if samples are processed but NOT 
embedding immediately after processing.
We deal with mostly mouse tissues and sometime samples will process over the 
weekend and come off on a day that no one can embed straight away.
Samples are taken out of the processor and let sit on the bench and the 
paraffin coated cassette tissues harden. This delay can be hours or a few days 
if it's a weekend/ holiday.  The samples are then placed in molten paraffin to 
melt and are  embedded.
We have done this with minimal problems but are wondering if some tissues like 
skin mouse ears  could be more difficult due to this delay. Have other lab done 
this delay or have seen any problems

Thank you for any though on this sticky situation...Hehe

Jamie

Jamie ericKson, MS
Scientist II
AbbVie Bioresearch Center, Inc.
Pharmacology/Pathology
100 Research Drive
Worcester, MA 01605
OFFICE+1 508-688-3134
EMAIL  jamie.erick...@abbvie.com
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