Re: [Histonet] FISH for breast core (Her2)
Hello Taganrog, If you are having issues with high autoflorescence due to prolonged formalin fixation (7 days), it might help to do a few minutes extra protease digestion (5+ minutes) during tissue pretreatment. Alternately or in conjunction, I would suggest re-applying HER2 probe to the same slide that had already been pretreated and hybridized. By doing this a few times (even up to four tries), you will likely get FISH signals that you can see and enumerate. Good luck, Mark Tarango On Tue, Jun 11, 2019 at 11:33 AM Пешков Максим via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Dear colleagues! > Can anyone help? We are very appreciatated any suggestions, tips and hacks > for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more > than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FISH for breast core (Her2)
Maxim, this is a link to the cIQc assessment page http://cpqa.ca/main/?page_id=24 If you look at the HER2 ISH assessments you will see numerous protocols used by laboratories. To my knowledge the protocols work on both biopsy and resection tissue. John www.ciqc.ca ‐‐‐ Original Message ‐‐‐ On Tuesday, June 11, 2019 11:18 AM, Пешков Максим via Histonet wrote: > > > Dear colleagues! > Can anyone help? We are very appreciatated any suggestions, tips and hacks > for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than > 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FISH for breast core (Her2)
Dear colleagues! Can anyone help? We are very appreciatated any suggestions, tips and hacks for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. Sincerely, Maxim Peshkov, Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
