Re: [Histonet] FoxP3 and Geminin Immunohistochemistry

[Hobbs, Carl]

​I have no xp re Geminin but, I have posted images of FoxP3 in FFPWS on the 
Histonet Images website.
The two abs work well for me.
I used a homemade std Citric acid HIER solution.
Looks like you have choices, given Katy Milne's response!

Good luck.

carl





  
  ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] FoxP3 and Geminin Immunohistochemistry

[Troutman, Kenneth A]

Hello Steven,

I have used both of these antibodies for various projects and we performed all 
of these on the Bondmax from Leica with a 60 minute antibody incubation.

We have used two FoxP3 antibodies from eBiosciences; cat# 14-4777 on human 
tissue and cat# 13-5773 for mouse tissue.  (both were 1:100 dilutions and ER2 
for 20 mins (EDTA pH9).

I have only used Geminin on human tissue.  It was from Proteintech Group cat# 
10802-1-AP (1:1500 dilution using ER1 for 15 min-ER1=citrate pH6)

All of these were on FFPE tissues for brightfield only.  We did not use them 
for IF.

If you need any other specifics, let me know.

Ashley Troutman BS, MBA, HT(ASCP)QIHC
Supervisor-Translational Pathology Shared Resource
Vanderbilt University Medical Center
S-1310 Medical Center North
1161 21st Avenue South
Nashville, TN  37232


Message: 2

Date: Tue, 2 Dec 2014 19:37:51 +

From: "Swartwood, Steven J" 
mailto:steven.swartw...@cshs.org>>

Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry

To: 
"histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>"


mailto:histonet@lists.utsouthwestern.edu>>

Message-ID:


<959202ac61aef942968646ec66e2be3e3bf09...@espwmsgmbx07.csmc.edu<mailto:959202ac61aef942968646ec66e2be3e3bf09...@espwmsgmbx07.csmc.edu>>

Content-Type: text/plain; charset="us-ascii"



Hello all,



I've done some research on Foxp3 and Geminin and I've seen a few antibodies 
that look sufficient for IHC in FFPE tissues. My lab is currently looking into 
purchasing these antibodies for a few different projects.



Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going 
to use them in bright field, but we may also turn to IF for other studies.



I'd be grateful for any information from what clone/company do you buy from and 
 retrieval methods to primary antibody incubations/dilutions.



Thank you all for any information,



Steven Swartwood HT(ASCP)

Cedars Sinai Medical Center

steven.swartw...@cshs.org<mailto:steven.swartw...@cshs.org>

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FoxP3 and Geminin Immunohistochemistry

[Swartwood, Steven J]

Hello all,

I've done some research on Foxp3 and Geminin and I've seen a few antibodies 
that look sufficient for IHC in FFPE tissues. My lab is currently looking into 
purchasing these antibodies for a few different projects.

Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going 
to use them in bright field, but we may also turn to IF for other studies.

I'd be grateful for any information from what clone/company do you buy from and 
 retrieval methods to primary antibody incubations/dilutions.

Thank you all for any information,

Steven Swartwood HT(ASCP)
Cedars Sinai Medical Center
steven.swartw...@cshs.org

IMPORTANT WARNING: This message is intended for the use of the person or entity 
to which it is addressed and may contain information that is privileged and 
confidential, the disclosure of which is governed by applicable law. If the 
reader of this message is not the intended recipient, or the employee or agent 
responsible for delivering it to the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of this information is 
strictly prohibited. Thank you for your cooperation.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet