RE: [Histonet] GFP antibody
another option is the antibody from NeoMarkers/Labvision MS-1288. I have used for several years mostly in mouse model but also works in other species and cell lines. have reference if you like + feel free if you have any questions -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anh2...@med.cornell.edu Sent: Thursday, March 05, 2009 7:08 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GFP antibody The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen and fixed frozen so it might work in paraffin. Worth a shot Andrea --Original Message-- From: Michele Wich Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mar 5, 2009 6:53 PM Subject: [Histonet] GFP antibody Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GFP Antibody
Hi, IĀ use Invitrogen's rabbit (A11122) GFP at 1:1000. Paula Pierce, BS, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N Broadway Ave Moore, OK 73160 405-759-3953 www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GFP antibody
The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen and fixed frozen so it might work in paraffin. Worth a shot Andrea --Original Message-- From: Michele Wich Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mar 5, 2009 6:53 PM Subject: [Histonet] GFP antibody Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GFP antibody
I am all ears to hear if anyone has gotten really good GFP signal after the FFPE process. I have not been able too. I have looked at a few articles but the images don't convince me. it seems to me that the processing squelches the GFP activity.. Michele Wich wrote: Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GFP antibody
Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet