Re: [Histonet] H. Pylori Testing

2015-04-29 Thread Paula Pierce 
Love, Love, Love the Sequenza. It is all I use.  Paula Pierce,BS, HTL(ASCP)HT 
President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 
405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com
  From: Amos Brooks 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Wednesday, April 29, 2015 4:48 PM
 Subject: [Histonet] H. Pylori Testing
   
Hi,
    If you are looking at the possibility of doing IHC manually, I would
really recommend the Shandon Sequenza. It is a box that holds clips that
attach to your slides. The slides are held vertically and you drop reagents
into the top. The reagents replace the previous reagent which drips into a
catch tray below. It makes it **really** easy to reproducibly do IHC
manually.

Amos Brooks


On Tue, Apr 28, 2015 at 12:24 PM,  wrote:

> Message: 8
> Date: Mon, 27 Apr 2015 16:49:25 -0400
> From: Garreyf 
> Subject: Re: [Histonet] H. Pylori Testing
> To: Michael Ann Jones 
> Cc: "histonet@lists.utsouthwestern.edu"
>        ,    "Vickroy, James"
>        
> Message-ID: 
> Content-Type: text/plain;      charset=us-ascii
>
> Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris
> in house as well. I'm trying to create more revenue to support a 2nd
> histotech.
>
> Garrey
>
> Sent from my iPhone
>
> > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones 
> wrote:
> >
> > We recently switched to IHC stain for HP, however, before that we used
> > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
> > Cell Marque by hand (with great results) and now we are automated.
> >
> > Michael Ann
> > Michael Ann Jones, HT (ASCP)
> > Histology Manager
> > Metropath
> > 7444 W. Alaska Dr. #250
> > Lakewood, CO 80226
> > 303.634.2511
> > mjo...@metropath.com
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] H. Pylori Testing

2015-04-29 Thread Amos Brooks 
Hi,
 If you are looking at the possibility of doing IHC manually, I would
really recommend the Shandon Sequenza. It is a box that holds clips that
attach to your slides. The slides are held vertically and you drop reagents
into the top. The reagents replace the previous reagent which drips into a
catch tray below. It makes it **really** easy to reproducibly do IHC
manually.

Amos Brooks


On Tue, Apr 28, 2015 at 12:24 PM,  wrote:

> Message: 8
> Date: Mon, 27 Apr 2015 16:49:25 -0400
> From: Garreyf 
> Subject: Re: [Histonet] H. Pylori Testing
> To: Michael Ann Jones 
> Cc: "histonet@lists.utsouthwestern.edu"
> ,"Vickroy, James"
> 
> Message-ID: 
> Content-Type: text/plain;   charset=us-ascii
>
> Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris
> in house as well. I'm trying to create more revenue to support a 2nd
> histotech.
>
> Garrey
>
> Sent from my iPhone
>
> > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones 
> wrote:
> >
> > We recently switched to IHC stain for HP, however, before that we used
> > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
> > Cell Marque by hand (with great results) and now we are automated.
> >
> > Michael Ann
> > Michael Ann Jones, HT (ASCP)
> > Histology Manager
> > Metropath
> > 7444 W. Alaska Dr. #250
> > Lakewood, CO 80226
> > 303.634.2511
> > mjo...@metropath.com
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re[2]: [Histonet] H. Pylori Testing

2015-04-28 Thread Brendal Finlay 
We do HP and Mart-1 testing in house by hand using concentrates.  It's a pretty 
basic set up using a pressure cooker, stain tray, pap pen, and the reagents. At 
this point in time it's less expensive than automation.  We start them around 
10 am and finish around 12 or 1 pm.  

  
Brendal C. Finlay, HT (ASCP)
Senior Histologist
Medical Center Clinic
8333 North Davis Highway
Pensacola, FL 32514
Phone 850.474.8581
Fax 850.474.8584 

-Original Message- 
From: Garreyf  
To: "Michael Ann Jones"  
Cc: histonet@lists.utsouthwestern.edu, "Vickroy, James" 
 
Date: 04/27/2015 15:49 
Subject: Re: [Histonet] H. Pylori Testing 

Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris in 
house as well. I'm trying to create more revenue to support a 2nd histotech.

Garrey

Sent from my iPhone

> On Apr 27, 2015, at 4:11 PM, Michael Ann Jones  wrote:
> 
> We recently switched to IHC stain for HP, however, before that we used
> Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
> Cell Marque by hand (with great results) and now we are automated.
> 
> Michael Ann
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
> 
> 
> 
> 
> 
> 
> On 4/27/15, 2:04 PM, "Vickroy, James" 
> wrote:
> 
>> 
>> We are a small lab processing mostly GI specimens.  Currently we are
>> sending our H. Pylori testing to a local hospital for staining however I
>> can predict that this year we may have around 1000 H. Pylori stains done.
>> I am looking for a small platform or manual test kit to perform the H.
>> Pylori's inhouse.   Can someone please give me their thoughts,
>> suggestions, or similar experiences  how they have or would approach this
>> endeavor?
>> 
>> Jim Vickroy
>> Histology Manager
>> Springfield Clinic, Main Campus, East Building
>> 1025 South 6th Street
>> Springfield, Illinois  62703
>> Office:  217-528-7541, Ext. 15121
>> Email:  
>> jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>
>> 
>> 
>> 
>> This electronic message contains information from Springfield Clinic, LLP
>> that may be confidential, privileged, and/or sensitive. This information
>> is intended for the use of the individual(s) or entity(ies) named above.
>> If you are not the intended recipient, be aware that disclosure, copying,
>> distribution, or action taken on the contents of this information is
>> strictly prohibited. If you have received this electronic message in
>> error, please notify the sender immediately, by electronic mail, so that
>> arrangements may be made for the retrieval of this electronic message.
>> Thank you.___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Michael Ann Jones 
If you do it by IHC it is the normal 2 hour or so process step-by-step.
Giemsa is quick 15 minutes or around there? Again, not as sensitive but
works.

Michael Ann




On 4/27/15, 2:49 PM, "Garreyf"  wrote:

>Is it a pain in the neck to do it  by hand? I'd like to bring my h
>pyloris in house as well. I'm trying to create more revenue to support a
>2nd histotech.
>
>Garrey
>
>Sent from my iPhone
>
>> On Apr 27, 2015, at 4:11 PM, Michael Ann Jones 
>>wrote:
>> 
>> We recently switched to IHC stain for HP, however, before that we used
>> Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
>> Cell Marque by hand (with great results) and now we are automated.
>> 
>> Michael Ann
>> Michael Ann Jones, HT (ASCP)
>> Histology Manager
>> Metropath
>> 7444 W. Alaska Dr. #250
>> Lakewood, CO 80226
>> 303.634.2511
>> mjo...@metropath.com
>> 
>> 
>> 
>> 
>> 
>> 
>> On 4/27/15, 2:04 PM, "Vickroy, James" 
>> wrote:
>> 
>>> 
>>> We are a small lab processing mostly GI specimens.  Currently we are
>>> sending our H. Pylori testing to a local hospital for staining however
>>>I
>>> can predict that this year we may have around 1000 H. Pylori stains
>>>done.
>>> I am looking for a small platform or manual test kit to perform the H.
>>> Pylori's inhouse.   Can someone please give me their thoughts,
>>> suggestions, or similar experiences  how they have or would approach
>>>this
>>> endeavor?
>>> 
>>> Jim Vickroy
>>> Histology Manager
>>> Springfield Clinic, Main Campus, East Building
>>> 1025 South 6th Street
>>> Springfield, Illinois  62703
>>> Office:  217-528-7541, Ext. 15121
>>> Email:  
>>> jvick...@springfieldclinic.com
>>> 
>>> 
>>> 
>>> This electronic message contains information from Springfield Clinic,
>>>LLP
>>> that may be confidential, privileged, and/or sensitive. This
>>>information
>>> is intended for the use of the individual(s) or entity(ies) named
>>>above.
>>> If you are not the intended recipient, be aware that disclosure,
>>>copying,
>>> distribution, or action taken on the contents of this information is
>>> strictly prohibited. If you have received this electronic message in
>>> error, please notify the sender immediately, by electronic mail, so
>>>that
>>> arrangements may be made for the retrieval of this electronic message.
>>> Thank you.___
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Garreyf 
Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris in 
house as well. I'm trying to create more revenue to support a 2nd histotech.

Garrey

Sent from my iPhone

> On Apr 27, 2015, at 4:11 PM, Michael Ann Jones  wrote:
> 
> We recently switched to IHC stain for HP, however, before that we used
> Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
> Cell Marque by hand (with great results) and now we are automated.
> 
> Michael Ann
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
> 
> 
> 
> 
> 
> 
> On 4/27/15, 2:04 PM, "Vickroy, James" 
> wrote:
> 
>> 
>> We are a small lab processing mostly GI specimens.  Currently we are
>> sending our H. Pylori testing to a local hospital for staining however I
>> can predict that this year we may have around 1000 H. Pylori stains done.
>> I am looking for a small platform or manual test kit to perform the H.
>> Pylori's inhouse.   Can someone please give me their thoughts,
>> suggestions, or similar experiences  how they have or would approach this
>> endeavor?
>> 
>> Jim Vickroy
>> Histology Manager
>> Springfield Clinic, Main Campus, East Building
>> 1025 South 6th Street
>> Springfield, Illinois  62703
>> Office:  217-528-7541, Ext. 15121
>> Email:  
>> jvick...@springfieldclinic.com
>> 
>> 
>> 
>> This electronic message contains information from Springfield Clinic, LLP
>> that may be confidential, privileged, and/or sensitive. This information
>> is intended for the use of the individual(s) or entity(ies) named above.
>> If you are not the intended recipient, be aware that disclosure, copying,
>> distribution, or action taken on the contents of this information is
>> strictly prohibited. If you have received this electronic message in
>> error, please notify the sender immediately, by electronic mail, so that
>> arrangements may be made for the retrieval of this electronic message.
>> Thank you.___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Michael Ann Jones 
We recently switched to IHC stain for HP, however, before that we used
Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
Cell Marque by hand (with great results) and now we are automated.

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 4/27/15, 2:04 PM, "Vickroy, James" 
wrote:

>
>We are a small lab processing mostly GI specimens.  Currently we are
>sending our H. Pylori testing to a local hospital for staining however I
>can predict that this year we may have around 1000 H. Pylori stains done.
> I am looking for a small platform or manual test kit to perform the H.
>Pylori's inhouse.   Can someone please give me their thoughts,
>suggestions, or similar experiences  how they have or would approach this
>endeavor?
>
>Jim Vickroy
>Histology Manager
>Springfield Clinic, Main Campus, East Building
>1025 South 6th Street
>Springfield, Illinois  62703
>Office:  217-528-7541, Ext. 15121
>Email:  
>jvick...@springfieldclinic.com
>
>
>
>This electronic message contains information from Springfield Clinic, LLP
>that may be confidential, privileged, and/or sensitive. This information
>is intended for the use of the individual(s) or entity(ies) named above.
>If you are not the intended recipient, be aware that disclosure, copying,
>distribution, or action taken on the contents of this information is
>strictly prohibited. If you have received this electronic message in
>error, please notify the sender immediately, by electronic mail, so that
>arrangements may be made for the retrieval of this electronic message.
>Thank you.___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] H. Pylori Testing

2015-04-27 Thread Vickroy, James 

We are a small lab processing mostly GI specimens.  Currently we are sending 
our H. Pylori testing to a local hospital for staining however I can predict 
that this year we may have around 1000 H. Pylori stains done.  I am looking for 
a small platform or manual test kit to perform the H. Pylori's inhouse.   Can 
someone please give me their thoughts, suggestions, or similar experiences  how 
they have or would approach this endeavor?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank 
you.___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet