Re: [Histonet] H. pylori RTU antibody
Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal. Which one do you use? Mark On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > Use the RTU from Biocare. It is clean and easy. You can use it with the > Dako Flex Kit. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [ > 41dm...@gmail.com] > Sent: Wednesday, June 30, 2010 10:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H. pylori RTU antibody > > I was just wondering if a lot of people use the RTU antibodies available > out > there for H. pylori. Currently, we're testing the RTU from Dako and we're > having issues with a lot of background staining. After tweaking the > protocol a lot, I've managed to get a lot of it removed, but there are > still > some issues. I was wondering if most people are making their own dilutions > to get a better stain or if the RTUs are commonplace. I appreciate the > feedback! > > Thanks, > Drew > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H. pylori RTU antibody
Use the RTU from Biocare. It is clean and easy. You can use it with the Dako Flex Kit. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [41dm...@gmail.com] Sent: Wednesday, June 30, 2010 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori RTU antibody I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori RTU antibody
I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
