Re: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
thanks Amos which one did you used ? you have catalog number ? On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks wrote: > Hi, > I am currently working on HMGB1. It is a weird one! If you go by the > data sheets you will get great nuclear staining on all the nuclei. This is > not what you are after if you have necrotic tissue. What you want to see is > the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I > am cutting the dilution to 1: 50 and 1:100 since we were just starting to > see blushes of cytoplasmic staining and no nuclear label at higher > dilutions. Usually you get staining that fades out at higher dilutions. This > one was really odd. > > Good luck, > Amos > > > Message: 13 > Date: Thu, 30 Sep 2010 04:55:02 +0200 > From: Fabrice GANKAM > Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > > Hey guys > Just wanted to know if any of you had some luck with an antibody aigainst > rat HMGB1 and its receptors RAGE, TLR2, TLR4. > W > Thanks > Dr Fabrice GANKAM > UTSW > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
Hi, I am currently working on HMGB1. It is a weird one! If you go by the data sheets you will get great nuclear staining on all the nuclei. This is not what you are after if you have necrotic tissue. What you want to see is the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I am cutting the dilution to 1: 50 and 1:100 since we were just starting to see blushes of cytoplasmic staining and no nuclear label at higher dilutions. Usually you get staining that fades out at higher dilutions. This one was really odd. Good luck, Amos Message: 13 Date: Thu, 30 Sep 2010 04:55:02 +0200 From: Fabrice GANKAM Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
