Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response. Thanks in advance. Sincerely Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong P.R.ChinaCell: +86-1312 8296 614
On Wednesday, 28 September 2016, 1:12, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. GMS (Martin, Gary) 2. Re: Unprocessing tissue (Caroline Miller) 3. Water for H&E Stainers? (P Sicurello) 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Sep 2016 16:30:49 -0700 From: "Martin, Gary" <gmar...@marshallmedical.org> To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] GMS Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary ------------------------------ Message: 2 Date: Mon, 26 Sep 2016 16:49:14 -0700 From: Caroline Miller <mi...@3scan.com> To: "Armstrong, Karoleigh T" <karoleigh.armstr...@armc.net> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Unprocessing tissue Message-ID: <CAFTHRQMn5=hqzoyf-frj-mf2agzeup_gzwyjxnhgux90hmk...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Caroline, > > This may sound a little crazy, > > but I was working on some mummified tissue back in the 80's, (Mommy is > old), and to deal with the dried out tissue we would give them, > > 1. A quick .5 percent Sodium Hydroxide soak, 5minutes > > 2. Wash is running water, 1 minute > > 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes > > 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. > > 5. Wash with running water 2-3 minutes. > > Proscess your tissue as you usually would. > > They came out pretty darn good. > > KK Armstrong H.T. (ASCP) > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello <pat...@gmail.com> To: HistoNet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Water for H&E Stainers? Message-ID: <capsjddyceta1fldsufgwzctayssqk6rxdrvtssqjmdwqaju...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 10:54:18 -0600 From: Elizabeth Chlipala <l...@premierlab.com> To: P Sicurello <pat...@gmail.com>, "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: <14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 22 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet