RE: [Histonet] How dark is dark enough?

[Morken, Timothy]

If you are using an automated stainer the plexiglas cover that they all have 
will block UV light. For instance we run all our IF in a Dako stainer with the 
slightly tinted Plexiglas and have not had any problems.  If staining manually 
in  a tray, covering with something to block light is ok. On the other hand, I 
can't really say I've had a problem even with no light blocking. The 
incubations are usually so short it may not make a difference. Consider that 
the focused UV light in the fluorescence scope is thousands of times stronger 
than any overhead tube and it still takes a while for the signal to dim. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, February 24, 2015 7:21 AM
To: HistoNet
Subject: [Histonet] How dark is dark enough?

Good Morning Netters,

While running immunofluorescence stains, how dark is dark enough?

I have worked in labs where we did them in full room light, almost complete 
darkness (developing negative/film darkness), and somewhere in between.

I feel that dimmed lights are good enough.

What does the histology collective think?

Thanks in advance!

Paula  :-)
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Re: [Histonet] How dark is dark enough?

[Rene J Buesa]

As long as your microscope has a good Hg source light, it is not really 
necessary to darken the room so much. I used to have a very small room were the 
microscope was located but later on we moved the microscope to another area 
were we did not dim the light at all and the vision was OK.Everything depends 
on the circumstances and how comfortable you are with the image.There are no 
rules on this subject.René J.  

 On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello  
wrote:
   

 Good Morning Netters,

While running immunofluorescence stains, how dark is dark enough?

I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.

I feel that dimmed lights are good enough.

What does the histology collective think?

Thanks in advance!

Paula  :-)
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[Histonet] How dark is dark enough?

[Paula Sicurello]

Good Morning Netters,

While running immunofluorescence stains, how dark is dark enough?

I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.

I feel that dimmed lights are good enough.

What does the histology collective think?

Thanks in advance!

Paula  :-)
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