Re: [Histonet] Ki67 antibody to stain Mouse tissue
Eva, et al, Yes, the LabVision/ThermoSci rabbit polyclonal does stain mouse tissue. I've done validation trials on various species with the antibody and mouse was one of many that did react. Jan Shivers UMN Vet Diag Lab On Wed, Aug 8, 2012 at 10:51 AM, Eva Permaul wrote: > I just found a nice publication with the Rm-9106 on mouse intestine. Have > provided it to my manager. Thank you everyone. > Eva > > On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote: > > > Just to clarify. Are you using it on mouse tissues? I am only asking > > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has > > only been verified on Human tissues. > > Secondly had anyone used it on mouse gut. Does it stain in the bottom of > > the crypts or at the top? I am asking because I have also tried a > different > > antibody from Leica but got staining at the top of the crypts not at the > > bottom. > > > > Thank you to everyone for all of your valuable suggestions and advice, > > Eva Permaul > > Georgetown University > > > > > > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < > > joost.bruijnt...@tno.triskelion.nl> wrote: > > > >> Hi Eva > >> > >> I've used an antibody from Labvision /Neomarkers. It is a rabbit > >> monoclonal; catalog number: RM-9106. > >> > >> Joost > >> > >> -Oorspronkelijk bericht- > >> Van: histonet-boun...@lists.utsouthwestern.edu [mailto: > >> histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul > >> Verzonden: maandag 6 augustus 2012 20:44 > >> Aan: histonet > >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue > >> > >> Hello, > >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako > >> M2749 but just found out it has been discontinued. Could anyone suggest > a > >> good alternative? > >> Thank you all for any help, > >> Eva Permaul > >> Georgetown University > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
FYI: That is what the data sheet says because they didn't test it but many of us have and it works beautifully! It should stain mostly in the bottom of the crypts where the new cells emerge from but you will have occasional cells towards the top of the crypt. It should look much like it does in human gut and yes, I have done it on most major organs of the mouse. Colleen Forster U of MN On 8/8/2012 10:38 AM, Eva Permaul wrote: Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < joost.bruijnt...@tno.triskelion.nl> wrote: Hi Eva I've used an antibody from Labvision /Neomarkers. It is a rabbit monoclonal; catalog number: RM-9106. Joost -Oorspronkelijk bericht- Van: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul Verzonden: maandag 6 augustus 2012 20:44 Aan: histonet Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
I just found a nice publication with the Rm-9106 on mouse intestine. Have provided it to my manager. Thank you everyone. Eva On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote: > Just to clarify. Are you using it on mouse tissues? I am only asking > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has > only been verified on Human tissues. > Secondly had anyone used it on mouse gut. Does it stain in the bottom of > the crypts or at the top? I am asking because I have also tried a different > antibody from Leica but got staining at the top of the crypts not at the > bottom. > > Thank you to everyone for all of your valuable suggestions and advice, > Eva Permaul > Georgetown University > > > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < > joost.bruijnt...@tno.triskelion.nl> wrote: > >> Hi Eva >> >> I've used an antibody from Labvision /Neomarkers. It is a rabbit >> monoclonal; catalog number: RM-9106. >> >> Joost >> >> -Oorspronkelijk bericht- >> Van: histonet-boun...@lists.utsouthwestern.edu [mailto: >> histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul >> Verzonden: maandag 6 augustus 2012 20:44 >> Aan: histonet >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue >> >> Hello, >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako >> M2749 but just found out it has been discontinued. Could anyone suggest a >> good alternative? >> Thank you all for any help, >> Eva Permaul >> Georgetown University >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < joost.bruijnt...@tno.triskelion.nl> wrote: > Hi Eva > > I've used an antibody from Labvision /Neomarkers. It is a rabbit > monoclonal; catalog number: RM-9106. > > Joost > > -Oorspronkelijk bericht- > Van: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul > Verzonden: maandag 6 augustus 2012 20:44 > Aan: histonet > Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue > > Hello, > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > M2749 but just found out it has been discontinued. Could anyone suggest a > good alternative? > Thank you all for any help, > Eva Permaul > Georgetown University > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
Just an FYI: Lab Vision antibodies HAVE to be ordered through Thermo Fisher. Lab Vision is a part of that company now. When you order antibodies from them you should use the Kalamazoo location as that is where the antibodies come from (I believe that is where Lab Vision was out of.) If you do this it will save you a LOT of headache. Colleen Forster U of MN On 8/6/2012 3:57 PM, Colleen Forster wrote: Thanks Jan, that would be the same one I have and it works great! Colleen Forster U of MN On 8/6/2012 2:59 PM, Jan Shivers wrote: We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag Lab On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: We are staining straight mouse tissue. On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: Are you staining human xenografts in mice or straight out mouse tissue? I get all mine from abcam. I am currently staining xenografts so I am using a human specific one, but I think it might be good for mouse too? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Monday, August 06, 2012 1:44 PM To: histonet Subject: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
Thanks Jan, that would be the same one I have and it works great! Colleen Forster U of MN On 8/6/2012 2:59 PM, Jan Shivers wrote: We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag Lab On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: We are staining straight mouse tissue. On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: Are you staining human xenografts in mice or straight out mouse tissue? I get all mine from abcam. I am currently staining xenografts so I am using a human specific one, but I think it might be good for mouse too? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Monday, August 06, 2012 1:44 PM To: histonet Subject: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ki67 antibody to stain Mouse tissue
Cell Marque's Ki-67 (SP6) works on mouse tissue. Please click on the link below for more information. http://www.cellmarque.com/cmc/P_antibodydetail.php?id=108&referrer=K Best Regards, Ms. Adrianna Eaton International Product Specialist 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8923 (707) 761-3270 (cell) (916) 746-8989 (fax) www.cellmarque.com From Passion to Product to Patient.® Learn with Cell Marque on: -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Monday, August 06, 2012 11:44 AM To: histonet Subject: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag Lab On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: > We are staining straight mouse tissue. > > On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: > > > Are you staining human xenografts in mice or straight out mouse tissue? > I > > get all mine from abcam. I am currently staining xenografts so I am > using > > a human specific one, but I think it might be good for mouse too? > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul > > Sent: Monday, August 06, 2012 1:44 PM > > To: histonet > > Subject: [Histonet] Ki67 antibody to stain Mouse tissue > > > > Hello, > > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > > M2749 but just found out it has been discontinued. Could anyone suggest a > > good alternative? > > Thank you all for any help, > > Eva Permaul > > Georgetown University > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
We are staining straight mouse tissue. On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: > Are you staining human xenografts in mice or straight out mouse tissue? I > get all mine from abcam. I am currently staining xenografts so I am using > a human specific one, but I think it might be good for mouse too? > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul > Sent: Monday, August 06, 2012 1:44 PM > To: histonet > Subject: [Histonet] Ki67 antibody to stain Mouse tissue > > Hello, > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > M2749 but just found out it has been discontinued. Could anyone suggest a > good alternative? > Thank you all for any help, > Eva Permaul > Georgetown University > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ki67 antibody to stain Mouse tissue
Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
