Re: [Histonet] Leica Bond III Opinions
Forwarding to Histonet: Hi Roger, The Bond IHC unit is used in two of the laboratories where I work. My hands-on experience is limited since I'm the new kid on the block but here's what I've learned, from my mistakes. The unit does not know if the covertiles are in place or not and the staining is awful without them. I don't think we have come up with an efficient way to effectively clean the covertiles. Rinsing or soaking in alcohol just doesn't do a very good job. I have spent way too much time soaking, wiping, and then rinsing. No broken slide trays yet so they are holding up to daily use. I know the pathologists are happy with the stain results. The techs are happy with the ease of use. Shorter run time was one of the things used to justify purchasing the Bond. The "open" system allows for use of antibodies not from Leica. I was not involved in the validation process so I don't know the specifics of choosing CellMarque or some of the other suppliers and for some reason OSCAR and one or two other protocols are still run on an older Ventana unit. The Bond requires a fair amount of daily and weekly maintenance but I think that's the norm for the more sophisticated stainers. These are my observations from one unit, in one lab. The other laboratory where I cut paraffin blocks a couple nights a week, has six Bonds of various generations. The only thing I can say about them is they must get the job done or there would not have been such a big investment. Take care, Dorothy In a message dated 2/6/2013 2:16:19 P.M. Eastern Standard Time, rhe...@lumc.edu writes: Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond III Opinions
The bond is great. A lot less waste to deal with and the maintenance is s easy. Less than 10 minutes. No tubing to unclog and disinfect. And the operating cost is much cheaper. And to top it off, it won't even give you the option to start a run until everything is right. No going back and forth to fix this, that, and whatever. It's truly a walk away system once you click start. Sent from the iPhone of Kim Tournear 🇺🇸 😃 On Feb 6, 2013, at 8:56 PM, Patsy Ruegg wrote: > Love the bond it does not break down very often for me and service is great i > can run all the antibodies i want for me even research animal because i have > a more open research unit cleaning the covertiles is a little tedious but we > make it work and replace them with new ones often beats disposing of all the > waste from liquid cs generated by ventana in my experience > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond III Opinions
Love the bond it does not break down very often for me and service is great i can run all the antibodies i want for me even research animal because i have a more open research unit cleaning the covertiles is a little tedious but we make it work and replace them with new ones often beats disposing of all the waste from liquid cs generated by ventana in my experience ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond III Opinions
Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond III
I want to poll Leica Bond III users to see if they are able to successfully run EBER Insitu on their unit? We continue to get inconsistent staining and inconsistent background staining. I have been told it's a pH problem, it's a tissue processing problem but we are able to do the stain with great results by hand. I welcome any and all suggestions __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond III
Hi Histonetters I am testing the Leica Bond III immunostainer. How is everyone handling processing the Hercept test from Dako on the Bond III? I cannot use either the approved pretreatment or visualization system, not to mention the approved buffer. I currently run my immunos on the Dako so it has not been a problem. I would appreciate any input anyone has. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
