Re: [Histonet] Mouse bladder
Exactly...and then it wont shrivel up C On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic wrote: > I agree with Colleen, processing is the issue. You need to process on a > longer program in order to dehydrate and clear the agar properly. > dusko > > > On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Be sure you are processing them on an overnight process. Even if the > samples are small it takes that time to process the agar properly. > > I use Histogel and learned this lesson the hard way. My samples always > "shriveled" up too until someone enlightened me about the processing time. > I would think agar might be the same. > > > Just a thought, > > Colleen Forster HT(ASCP)QIHC > U of MN > > On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > I would try histogel instead of agar. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > l...@premierlab.com<mailto:l...@premierlab.com> > > www.premierlab.com<http://www.premierlab.com/> > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g> > > Longmont, CO 80504 > <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g> > > > > From: Heather Marlatt via Histonet [mailto:histonet@lists. > > utsouthwestern.edu] > > Sent: Tuesday, December 19, 2017 9:12 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Mouse bladder > > > > Does anyone have a good protocol for mouse bladder embedded in agar that > > they are willing to share? We have mouse bladders in 2% agar and have > tried > > a few different protocol variations from our usual mouse but the agar > keeps > > shriveling up. > > > > Thanks > > Heather > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists. > utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mouse bladder
I agree with Colleen, processing is the issue. You need to process on a longer program in order to dehydrate and clear the agar properly.dusko On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet wrote: Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly. I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com<mailto:l...@premierlab.com> > www.premierlab.com<http://www.premierlab.com/> > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > From: Heather Marlatt via Histonet [mailto:histonet@lists. > utsouthwestern.edu] > Sent: Tuesday, December 19, 2017 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mouse bladder > > Does anyone have a good protocol for mouse bladder embedded in agar that > they are willing to share? We have mouse bladders in 2% agar and have tried > a few different protocol variations from our usual mouse but the agar keeps > shriveling up. > > Thanks > Heather > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mouse bladder
Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly. I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com<mailto:l...@premierlab.com> > www.premierlab.com<http://www.premierlab.com/> > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > From: Heather Marlatt via Histonet [mailto:histonet@lists. > utsouthwestern.edu] > Sent: Tuesday, December 19, 2017 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mouse bladder > > Does anyone have a good protocol for mouse bladder embedded in agar that > they are willing to share? We have mouse bladders in 2% agar and have tried > a few different protocol variations from our usual mouse but the agar keeps > shriveling up. > > Thanks > Heather > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mouse bladder
I would try histogel instead of agar. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com<mailto:l...@premierlab.com> www.premierlab.com<http://www.premierlab.com/> Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Heather Marlatt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, December 19, 2017 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse bladder Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse bladder
Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet