Re: [Histonet] Not DAB IHC waste
Mehndi: I’ve used products like the ones referring to in many labs, over many years, and although it’s difficult to find explicit references stating that Fast Red (and Fast Blue)-type chromogens are truly hazardous/carcinogenic, like DAB, these are all very complex chemicals that we, as responsible scientists and citizens, should probably refrain from putting into the public sewer system/water supply. I would, therefore, recommend that your lab treat such waste-reagents as hazardous and dispose of them accordingly. Respectfully, Joe Myers, M.S., CT(ASCP)QIHC Message: 6 Date: Fri, 22 Feb 2019 15:34:36 + From: Histology To: "histonet@lists.utsouthwestern.edu" Hi all, Does anyone use only red or magenta detection for IHCs instead of DAB? We do not use DAB and are trying to figure out if our waste is hazardous or not. Any insight would be helpful. Thanks, Mehndi Helgren ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Not DAB IHC waste
Hello, It depends upon which 'red' chromogen you are using. There are several with different chemical compositions, so you will need to look at the Safety Data Sheets which pertain to the chemical you are using. In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered hazardous. It causes eye and skin irritation, may cause serious eye damage, and may cause cancer. We ship our waste off for proper disposal; it does not go down the water drain. Best regards, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Fri, Feb 22, 2019 at 9:34 AM Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Does anyone use only red or magenta detection for IHCs instead of DAB? We > do not use DAB and are trying to figure out if our waste is hazardous or > not. Any insight would be helpful. > > Thanks, > > Mehndi Helgren > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Not DAB IHC waste
Hi all, Does anyone use only red or magenta detection for IHCs instead of DAB? We do not use DAB and are trying to figure out if our waste is hazardous or not. Any insight would be helpful. Thanks, Mehndi Helgren ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
