Re: [Histonet] Permanent mountant for Oil Red O

[B kB via Histonet]

Hi Christopher,

You van try Imsol-mount
Vendors are for example Sigmaaldrich or VWR .

After a staining, airdry the solide. Cover with mislopen-mount and let this
dry for a while ( no problem with higher temps). After that, You can cover
it with glass or tape.

Goed luck,
Bert klein brink
Gelre Hospital, Apeldoorn, Netherlands



Op ma 16 sep. 2019 om 06:04 schreef Hagon, Christopher (Health) via
Histonet 

> UNCLASSIFIED
>
> Hello Histonetters,
>
> We do Oil Red O stains on frozen section post mortem tissue, and need to
> find a permanent aqueous mounting medium. We used to use the Thermo-Fisher
> Perm-mount, but can't seem to get it anymore. As they are looking for fat
> deposits, we can't use any solvent based solutions, and the only aqueous
> ones we've found aren't permanent. We have to send the slides off site
> after staining, so the chances of the coverslip moving in transport is
> fairly high.
>
> Does anyone else have this issue and what did you end up using?
>
> Thanks in advance,
>
> Chris Hagon | Senior Scientist, Anatomical Pathology
> ACT Pathology | health.act.gov.au
> Phone (02) 5124 2874
>
>
>
>
>
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> should not copy or use it for any purpose, nor disclose its contents to any
> other person.
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Re: [Histonet] Permanent mountant for Oil Red O

[John Kiernan via Histonet]

Fructose syrup gives a hard set, but it's a bit acidic (I don't know why) and 
is therefore incompatible with simple basic dyes, but it's OK for Oil red O and 
the Sudans.
   Fructose (also called laevulose or levulose)  15 g
   Distilled water  5 ml
Leave at ~60C (in paraffin oven) for 2 or 3 days for all the fructose to 
dissolve. Don't let the water evaporate!  The transparent syrup keeps well for 
2-3 months at room temp.
With long storage fructose crystallizes under the coverslip; this can be 
retarded by sealing the adges with a resinous mounting medium such as DPX. (You 
can buy fructose powder for cooking - about $3 a pound on the the internet. Cf  
$25-80 from chemical supply houses.)

Another good one is polyvinylpyrrolidone:
 PVP (m.w. 10,000)   25g
 Water (or a phosphate buffer pH 7.4) 25ml
  When dissolved (several hours, magnetic stirring), add 1ml glycerol and a 
small crystal of thymol.
Keeps for up to 3 years. Discard if it becomes cloudy. Use the buffered variety 
if the preparation has been stained or counterstained with a basic dye like 
toluidine blue or neutral red.
This is less viscous than fructose syrup, and also has a lower refractive 
index, but with evaporation at the edges of the coverslip it gradually (weeks) 
becomes harder, and its refractive index increases almost to that of a resinous 
mountant.  Probably you won't want to wait before shipping the slides 
elsewhere.  PVP costs more than fructose (about $150 per pound for PVP10).

This short article about aqueous mounting media is rather old (1997) but 
probably still OK:
http://publish.uwo.ca/~jkiernan/aqmount.htm

John Kiernan
London, Canada
= = =


From: Hagon, Christopher (Health) via Histonet 

Sent: 15 September 2019 22:43
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Permanent mountant for Oil Red O

UNCLASSIFIED

Hello Histonetters,

We do Oil Red O stains on frozen section post mortem tissue, and need to find a 
permanent aqueous mounting medium. We used to use the Thermo-Fisher  
Perm-mount, but can't seem to get it anymore. As they are looking for fat 
deposits, we can't use any solvent based solutions, and the only aqueous ones 
we've found aren't permanent. We have to send the slides off site after 
staining, so the chances of the coverslip moving in transport is fairly high.

Does anyone else have this issue and what did you end up using?

Thanks in advance,

Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>
Phone (02) 5124 2874





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person.
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[Histonet] Permanent mountant for Oil Red O

[Hagon, Christopher (Health) via Histonet]

UNCLASSIFIED

Hello Histonetters,

We do Oil Red O stains on frozen section post mortem tissue, and need to find a 
permanent aqueous mounting medium. We used to use the Thermo-Fisher  
Perm-mount, but can't seem to get it anymore. As they are looking for fat 
deposits, we can't use any solvent based solutions, and the only aqueous ones 
we've found aren't permanent. We have to send the slides off site after 
staining, so the chances of the coverslip moving in transport is fairly high.

Does anyone else have this issue and what did you end up using?

Thanks in advance,

Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au
Phone (02) 5124 2874





---
This email, and any attachments, may be confidential and also privileged. If 
you are not the intended recipient, please notify the sender and delete all 
copies of this transmission along with any attachments immediately. You should 
not copy or use it for any purpose, nor disclose its contents to any other 
person.
---
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