Feulgen demonstrates the deoxyribose which is in the backbone of each helical strand. It shouldn't matter if single or double stranded. Hydrolysis is the key step and can be overdone. Textbooks list using hot 1N HCl Many years ago Jules Elias and I co-authored a paper where we demonstrated that the use of 5N HCl at room temperature provided more consistent results
Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 Learn more about Histotechnology Professionals Day at www.nsh.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, January 07, 2011 4:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Feulgen Stain for DNA Hello Everyone I have tried to find this answer on the web and in references but I need some help. With respects to the Feulgen stain, how sensitive is it? Can it detect smaller fragments of DNA? Does it have to be double stranded or will single stranded stain as well? Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet