We use the platinum series from Mercedes Medical - they are so
hydrophilic, that if you place your section wrong on the slide, you cannot
float it off and start over. We also take our sections at a backwards
angle from the surface of the water: rather than lift from underneath - we
put the slide in the water bath next to the section face down at a severe
angle, attach the section to the top of the slide (or wherever) and then
pull slide out at angle. This encourages the water to stay in the bath and
not under sections. Does that make sense?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 11/11/14, 10:48 AM, "Richard Bowker" wrote:
>Judith,
>
>When you pick up your sections are you getting a lot of water trapped
>between the tissue and the slides. I have had that problem when getting a
>new lot number of slides before and had to change them out. The tissue
>wasn't actuall falling off rather then being pushed off after the water
>begins to heat up and expand forcing the tissue off. In this instance
>shaking the slide after picking up the tissue had no benifit either as
>this also caused the water to force the tissue off the slides.
>
>Rick Bowker
>Histology supervisor
>Olympia Multi-Specialty Clinic
>richa...@omsc.net
>
>Message: 5
>Date: Sat, 8 Nov 2014 13:31:27 +0300
>From: "Jamal"
>Subject: RE: [Histonet] Tissue falling off positive slides
>To: "'Pardue, Judith'" ,
>
>Message-ID:
><00c001cffb3f$297ea1f0$7c7be5d0$@rowa...@alborglaboratories.com>
>Content-Type: text/plain; charset="windows-1256"
>
>Do you use Autostainer?
>What brand ?
>
>
>Best Regards,
>
>
>Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
>Medical Laboratories |? Mobile +966 503629832|
>j.rowa...@alborglaboratories.com
>Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
>Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
>www.alborglaboratories.com
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue,
>Judith
>Sent: Thursday, November 06, 2014 6:38 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Tissue falling off positive slides
>
>WE are having trouble with tissue coming off our h&e slides. Our heater is
>set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue
>positive slides.
>
>Judith Pardue
>CHI Memorial
>Chatt. Tn. 37343
>judith_par...@memorial.org
>
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