Re: [Histonet] RE: Three shades of Eosin
Ok, I can't help myselfbut all this talk is reminding me of " 50 shades of grey" (can't put this on Histonet) I'll take my "HistoPorn" any day of the week over 50 shades! All the pretty pictures under the scope. That is my passion~ I get my "3 shades of eosin" with Richard Allan eosin Y. Wanda K Simons, HT(ASCP) > ---Original Message--- > From: Michael Ann Jones > To: Terri Braud , histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] RE: Three shades of Eosin > Sent: Sep 11 '14 15:03 > > Terri - agreed. We do the same. > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > mjo...@metropath.com > > > > > On 9/11/14, 12:42 PM, "Terri Braud" wrote: > > >Eosin is differentiated by water content immediately following the Eosin > >stain. This can be accomplished by a water rinse, or rinse in a diluted > >alcohol. I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of > >Absolute, all timed to give the desired differentiation for the 3 > >desired shades. > > > >Terri L. Braud, HT(ASCP) > >Anatomic Pathology Supervisor > >Holy Redeemer Hospital Laboratory > >1648 Huntingdon Pike > >Meadowbrook, PA 19046 > >Ph: 215-938-3676 > >Fax: 215-938-3874 > > > >Sent: Thursday, September 11, 2014 12:09 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: Histonet Digest, Vol 130, Issue 11 > > > > 8. Eosin shades (Manahil) > >Date: Thu, 11 Sep 2014 20:15:21 +0400 > >From: Manahil > >Subject: [Histonet] Eosin shades > >Hi every one > >Any one know how to get 3 shades of eosin stain. I am using alcoholic > >eosin. > >Thanks > >Manahil Elbireir > >HTL/ ASCP > >-- > >--- > > > > > > > >CONFIDENTIALITY NOTICE: > > > >This E-Mail is intended only for the use of the individual or entity to > >which > >it was sent. It may contain information that is privileged and/or > >confidential, > >and the use or disclosure of such information may also be restricted > >under applicable > >federal and state law. If you received this communication in error, > >please do not > >distribute any part of it or retain any copies, and delete the original > >E-Mail. > >Please notify the sender of any error by E-Mail. > > > >Thank you for your cooperation. > > > > > >___ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Three shades of Eosin
Terri - agreed. We do the same. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 9/11/14, 12:42 PM, "Terri Braud" wrote: >Eosin is differentiated by water content immediately following the Eosin >stain. This can be accomplished by a water rinse, or rinse in a diluted >alcohol. I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of >Absolute, all timed to give the desired differentiation for the 3 >desired shades. > >Terri L. Braud, HT(ASCP) >Anatomic Pathology Supervisor >Holy Redeemer Hospital Laboratory >1648 Huntingdon Pike >Meadowbrook, PA 19046 >Ph: 215-938-3676 >Fax: 215-938-3874 > >Sent: Thursday, September 11, 2014 12:09 PM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 130, Issue 11 > > 8. Eosin shades (Manahil) >Date: Thu, 11 Sep 2014 20:15:21 +0400 >From: Manahil >Subject: [Histonet] Eosin shades >Hi every one >Any one know how to get 3 shades of eosin stain. I am using alcoholic >eosin. >Thanks >Manahil Elbireir >HTL/ ASCP >-- >--- > > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to >which >it was sent. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted >under applicable >federal and state law. If you received this communication in error, >please do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail. > >Thank you for your cooperation. > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Three shades of Eosin
Eosin is differentiated by water content immediately following the Eosin stain. This can be accomplished by a water rinse, or rinse in a diluted alcohol. I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of Absolute, all timed to give the desired differentiation for the 3 desired shades. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Sent: Thursday, September 11, 2014 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 130, Issue 11 8. Eosin shades (Manahil) Date: Thu, 11 Sep 2014 20:15:21 +0400 From: Manahil Subject: [Histonet] Eosin shades Hi every one Any one know how to get 3 shades of eosin stain. I am using alcoholic eosin. Thanks Manahil Elbireir HTL/ ASCP - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
