Re: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue
I use the anti CD68 clone FA-11 ( obtained from Abcam). It works very well on frozen sections but, I have been unable to get any positivity on Pwax sections ( using +/- Citric acid pH6 HIER) Carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue
Has anyone tried these Abs? If so, please send me your beautiful photos and protocol. Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology WIMR I Rm. 4012 Highland Av. Madison, WI 53705 (608)262-1836 From: Gayle Callis Sent: Wednesday, May 13, 2015 12:34 PM To: Histonet Subject: [Histonet] RE. murine CD4, CD8 and CD68 for FFPE tissue You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ** Thankfully and at long last after years of frustration by many eBioscience has both the CD8a and CD4 which is now available for FFPE murine tissues. Read the data sheets carefully as the CD4 indicated this antibody was to be used on FFPE and not frozen sections. Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as: CD8 alpha, Ly-2, Ly-35, Lyt-2 Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as: L3T4, Ly-4 Serotec has a rat anti mouse CD68 where the data sheet says can be used on FFPE murine tissue. This clone must be available from other companies as well. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue
You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ** Thankfully and at long last after years of frustration by many eBioscience has both the CD8a and CD4 which is now available for FFPE murine tissues. Read the data sheets carefully as the CD4 indicated this antibody was to be used on FFPE and not frozen sections. Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as: CD8 alpha, Ly-2, Ly-35, Lyt-2 Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as: L3T4, Ly-4 Serotec has a rat anti mouse CD68 where the data sheet says can be used on FFPE murine tissue. This clone must be available from other companies as well. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
