[Histonet] Re: [Sectioning] Brain Tissue
We keep our water bath at 40-41 C. Sometimes we add some 70% ethanol to the water bath and this helps to get the wrinkles out (especially for cerebellum and mice brain). Most of our tissue is bovine brain but we deal with multiple other species including mice. We use McCormick Paraplast Plus embedding Medium from Fisher. Nancy Herman Canadian Food Inspection Agency - Lethbridge Laboratory >>> "Sherwood, Margaret " 2010/07/27 9:14 am >>> To All: We have been experiencing problems sectioning mouse brain. The sectioning is fine, but we have problems in the water bath. At 45 degrees C (peel-a-way paraffin-Polysciences), the sections break apart but we don't get wrinkles. With paraplast extra, we use 36 degrees C, but the sections are wrinkled and disintegrate. I would appreciate hearing from listers as to what you do: what temperature is your water bath, what embedding media do you use, etc? Any help would be appreciated! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: [Sectioning] Brain Tissue
My water bath is usually around the same temperature as yours is.= I
just take literally a hand full of ice and put it in the bath, onc= e
all the ice melts, I cut the brain. I'll try to go look at what tem p
it gets to, my guess would be around room temperature? Your
section= s are "blowing up" because the bath is just too hot.
<= br>
Sarah Goebel, B= .A., HT (ASCP)
Histotechnician
<= /em>
XBiotech USA I= nc.
8201 East Riverside Dr. Bldg 4 Suite 100
Aust= in, Texas 78744
(512)386-5107
Original Message --------
Subject: [Histonet] Re: [Sectioning] Brain Tissue
From: "Sherwood, Margaret " <[1]msherw...@partners.org>
Date: Tue, July 27, 2010 8:14 am
To: <[2]histo...@lis= ts.utsouthwestern.edu>
To All:
We have been experiencing problems sectioning mouse brain. The
sectioning = is
fine, but we have problems in the water bath. At 45 degrees C
(peel-a-way<= br> paraffin-Polysciences), the sections break apart but
we don't get wrinkles. With paraplast extra, we use 36 degrees C,
but the sections are
wrinkled an= d
disintegrate.
I would appreciate hearing from listers as to what you do: what
temperatur= e is
your water bath, what embedding media do you use, etc? Any help would
be appreciated!
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
[3]msherw...@partners.org
The information in this e-mail is intended only for the person to whom
it i= s
addressed. If you believe this e-mail was sent to you in error and the
e-ma= il
contains patient information, please contact the Partners Compliance
HelpLi= ne at
[4]http://www.partners.org/= complianceline . If the e-mail was sent
to you in error
but does not contain patient information, please contact the sender
and pro= perly
dispose of the e-mail.
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References
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[Histonet] Re: [Sectioning] Brain Tissue
To All: We have been experiencing problems sectioning mouse brain. The sectioning is fine, but we have problems in the water bath. At 45 degrees C (peel-a-way paraffin-Polysciences), the sections break apart but we don't get wrinkles. With paraplast extra, we use 36 degrees C, but the sections are wrinkled and disintegrate. I would appreciate hearing from listers as to what you do: what temperature is your water bath, what embedding media do you use, etc? Any help would be appreciated! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
