Re: [Histonet] Smad4 (B-8) / DPC4
Our SMAD-4 is run on the Biocare Intellipath using the Santa Cruz B-8 clone. We do Diva retrieval, 1:200 dilution for 30 minutes followed by PeroxAbolish to eliminate non-specific background staining. Our pathology group has been happy with this. On Tue, Mar 6, 2012 at 5:34 AM, Angela Bitting wrote: > I've tried to work it up on the XT also with inconsistent, weak results. > If you get a reply on this one, let me know. > > Angie > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > > >>> "Giroux, Stacy" 3/6/2012 7:35 AM >>> > Hi, > > I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are > currently trying to work up this antibody for validation; however, we are > having significant problems getting it to work consistently. We are using > the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If > anyone has protocol information to share regardless of platform used it > would be greatly appreciated. > > Thanks, > Stacy > > > Stacy Giroux, HTL(ASCP) > Operations Coordinator, Histology > St. John Hospital & Medical Center > Phone: 313-343-3130 > Fax: 313-343-4965 > > Histotechnology Professionals Day - March 10, 2012 > > > CONFIDENTIALITY NOTICE: > This email message and any accompanying data or files is confidential and > may contain privileged information intended only for the named > recipient(s). If you are not the intended recipient(s), you are hereby > notified that the dissemination, distribution, and or copying of this > message is strictly prohibited. If you receive this message in error, or > are not the named recipient(s), please notify the sender at the email > address above, delete this email from your computer, and destroy any copies > in any form immediately. Receipt by anyone other than the named > recipient(s) is not a waiver of any attorney-client, work product, or other > applicable privilege.___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It > is intended solely for the addressee. Access to this message by anyone else > is unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Smad4 (B-8) / DPC4
I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know. Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Giroux, Stacy" 3/6/2012 7:35 AM >>> Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Smad4 (B-8) / DPC4
Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
