Allyse,
I used to have those problems, too. I tried JB-4 and T-7100 and L.R. White.
Sometimes I had great results, sometimes not. The results were never
consistent. Then one glorious day, someone from another lab suggested I try
Immuno-bed. It is amazing. I have not had those issues ever again. It sets
up so quickly. If I make and use the embedding solution in the morning, I can
trim and glue my samples to the chuck later that same day. Unbelievable! I
get it from Poly-Sciences. Please try it!
Nancy Thomas
Stowers Institute for Medical Research
Kansas City, MO
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Allyse
Mazzarelli
Sent: Wednesday, August 15, 2012 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Specimen Blocks too Soft to Cut
This question pertains to anyone that uses the Technovit 7100 kit. My lab has
seen problems with humidity recently... (lab is too big to use a dehumidifier).
I embed with enough hardener and mix my embedding solution well, but when I
take my samples out of the mold they are still soft. I have tried to store them
in an air tight container in dessicant beads over night, but that only
partially works. Any other suggestions? Has anyone else that uses this kit ran
into a similar problem? Thanks in advance to anyone that can send me some
advice!!
Sincerely,
Allyse Mazzarelli
NEUROTECH USA INC.
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