RE: [Histonet] Thyroid Smears
Fawn: Fixation of exceptionally 'fluid' cytologic specimens, like many thyroid aspirates, through a submersion technique will nearly always result in a significant loss of material from the slides. And less important than the loss of what appears to be blood is the loss of thyroid epithelial cells/tissue fragments. If the amount of blood in the specimen appears, grossly, to be 'excessive', then, as Debbie suggested, adding PlasmaLyte to the fluid prior to smear preparation is a very practical option. Assuming that the smears will be Pap-stained, then the best way to prepare them is to express one or two drops of the aspirate onto a slide, spread the material using another slide, spray-fix both smears with (a commercially-prepared solution containing primarily) ethanol, and then air dry them thoroughly before staining. And if some of the smears will be immuno-stained in a protocol designed/validated for histologic material, these slides may be spray-fixed with 10% formalin instead of ethanol. Good Luck, Joe Myers, M.S., CT(ASCP)QIHC -- Message: 9 Date: Wed, 12 Jan 2011 09:33:06 -0500 From: Fawn Bomar Subject: [Histonet] Thyroid Smears To: "histonet@lists.utsouthwestern.edu" Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thyroid Smears
I agree with Sharon, that's what we use and never really have a problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 12, 2011 11:02 AM To: 'IRENA SREBOTNIK KIRBIS'; fawn.bo...@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears Try using plus slides and make the smears very thin. Then it won't matter what kind of fixative you are using everything will stick. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, January 12, 2011 10:55 AM To: fawn.bo...@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: fawn.bo...@halifaxregional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of > thyroid smears. As of right now, we go up to the room and collect the thyroid > sample. The Pathologist makes the smears in the room and immediately puts > them into 95% Isopropanol to fix. We then complete the stain later on in the > day. The problem that we are encountering is that all of the blood and cells > are coming off of the slides before we make it through the entire stain. Does > anyone have any suggestions or are willing to share the procedure that they > use? We had a couple of suggestions that we recommended to the Doctor but > they were dismissed. I don't want to tell what are suggestions were so that > the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > - > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thyroid Smears
Try using plus slides and make the smears very thin. Then it won't matter what kind of fixative you are using everything will stick. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, January 12, 2011 10:55 AM To: fawn.bo...@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: fawn.bo...@halifaxregional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of > thyroid smears. As of right now, we go up to the room and collect the thyroid > sample. The Pathologist makes the smears in the room and immediately puts > them into 95% Isopropanol to fix. We then complete the stain later on in the > day. The problem that we are encountering is that all of the blood and cells > are coming off of the slides before we make it through the entire stain. Does > anyone have any suggestions or are willing to share the procedure that they > use? We had a couple of suggestions that we recommended to the Doctor but > they were dismissed. I don't want to tell what are suggestions were so that > the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > - > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thyroid Smears
I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: fawn.bo...@halifaxregional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of > thyroid smears. As of right now, we go up to the room and collect the thyroid > sample. The Pathologist makes the smears in the room and immediately puts > them into 95% Isopropanol to fix. We then complete the stain later on in the > day. The problem that we are encountering is that all of the blood and cells > are coming off of the slides before we make it through the entire stain. Does > anyone have any suggestions or are willing to share the procedure that they > use? We had a couple of suggestions that we recommended to the Doctor but > they were dismissed. I don't want to tell what are suggestions were so that > the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > - > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thyroid Smears
Our procedure is as such: The FNA of the thyroid is place in 5mls of plasmalyte ( an electrolyte solution). We lyse the red blood cells and make cytospins. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Fawn Bomar Sent by: histonet-boun...@lists.utsouthwestern.edu 01/12/2011 09:37 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Thyroid Smears Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thyroid Smears
We always used 95% alcohol to fix, but you're right the blood usually comes off. Air dry then diff quik (or giemsa) stain works better for a hema-pathologic stain. You could try Pen Fix (or an alcoholic formalin) and this might help as opposed to the 95%. Why does the pathologist need to see blood cells in a thyroid asp.? Just my 2 cents =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, January 12, 2011 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thyroid Smears Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thyroid Smears
Tell the pathologist to stop fixing the smears. Let the smears air dry and later you fix/stain them. Besides fixing with 2-propanol is not the way to fix. If they also dismiss this procedure, then the will end without stained smears. René J. --- On Wed, 1/12/11, Fawn Bomar wrote: From: Fawn Bomar Subject: [Histonet] Thyroid Smears To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 12, 2011, 9:33 AM Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thyroid Smears
Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
