Re: [Histonet] Whole human feet
Thanks Rene, I have heard of the sliding microtomes and a colleague thought that as long as I could fix and decalcify, it might be possible with one of these. I fear I may have to pre-cut the foot in to manageable portions before processing. Yes, if I find out how to do it I will definitely let you know! Yak-Nam On Fri, Apr 10, 2009 at 1:34 PM, Rene J Buesa wrote: > As far as I know such a large thin section that also involves decalcifying > hard bones has never been published. In the past E. Leitz (now Leica) > manufactured sliding microtomes for large specimens but were used mostly to > prepare thin brain sections embedded in celloidin but not for what you would > like to do. > If you are able to do it, for sure we all would like to know. > René J. > > --- On *Fri, 4/10/09, Yak-Nam Wang * wrote: > > From: Yak-Nam Wang > Subject: Re: [Histonet] Whole human feet > To: "Woodward, Denise" > Cc: "histonet@lists.utsouthwestern.edu" > > Date: Friday, April 10, 2009, 4:26 PM > > > Denise, > > Thanks, someone else pointed out these images and they are indeed in the > correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I > think these are 1 mm sections from a frozen body, unstained. We were > actually hoping that we could process feet (procured from a company), > section and stain the sections with the basic H&E, modified Hart's, > Picro > sirius red and such like. > > Any help would be greatly appreciated. > Thanks > Yak-Nam > > On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise > wrote: > > > I believe there is a computerized image file of an entire male and a > > female human body somewhere on the WWW. Maybe NIH?? These were whole > mount > > histologic sections. Don't know if the plane is correct for your needs > with > > regard to the sections of feet. News about it came out about 4-5 years > ago. > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > > Sent: Wednesday, April 08, 2009 12:07 AM > > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Whole human feet > > > > No offense, dude, but GROSS. > > > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > > > > > > > From: Yak-Nam Wang > > To: histonet@lists.utsouthwestern.edu > > Sent: Monday, April 6, 2009 8:04:54 PM > > Subject: [Histonet] Whole human feet > > > > Hello all, > > > > If possible, we would like to obtain histological sections of adult human > > feet (plane of the anterior-posterior surface). Does anyone know of > > labs/groups that have done this or something similar? > > > > Thank you for your help > > Yak-Nam Wang > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing > listhisto...@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Whole human feet
Virtual autopsy web site? They have the whole body ct scanned and sliced. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Friday, April 10, 2009 3:11 PM To: 'Bernie Taupin'; Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Whole human feet I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
As far as I know such a large thin section that also involves decalcifying hard bones has never been published. In the past E. Leitz (now Leica) manufactured sliding microtomes for large specimens but were used mostly to prepare thin brain sections embedded in celloidin but not for what you would like to do. If you are able to do it, for sure we all would like to know. René J. --- On Fri, 4/10/09, Yak-Nam Wang wrote: From: Yak-Nam Wang Subject: Re: [Histonet] Whole human feet To: "Woodward, Denise" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, April 10, 2009, 4:26 PM Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic H&E, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise wrote: > I believe there is a computerized image file of an entire male and a > female human body somewhere on the WWW. Maybe NIH?? These were whole mount > histologic sections. Don't know if the plane is correct for your needs with > regard to the sections of feet. News about it came out about 4-5 years ago. > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > Sent: Wednesday, April 08, 2009 12:07 AM > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Whole human feet > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > From: Yak-Nam Wang > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic H&E, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise wrote: > I believe there is a computerized image file of an entire male and a > female human body somewhere on the WWW. Maybe NIH?? These were whole mount > histologic sections. Don't know if the plane is correct for your needs with > regard to the sections of feet. News about it came out about 4-5 years ago. > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > Sent: Wednesday, April 08, 2009 12:07 AM > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Whole human feet > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > From: Yak-Nam Wang > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Whole human feet
I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Correction, we would like the cut to be dorsal to plantar surface (e.g. through the metatarsals), not anterior to posterior (that would be a rather large section!) Cheers! Yak-Nam On 4/6/09 5:04 PM, "Yak-Nam Wang" wrote: > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole human feet
Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
